##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933342.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	656361
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.667151156147302	31.0	28.0	33.0	25.0	34.0
2	29.147220812936784	31.0	28.0	33.0	16.0	34.0
3	29.04138880890242	31.0	28.0	34.0	16.0	34.0
4	33.444119927905525	35.0	32.0	37.0	28.0	37.0
5	34.41480526722337	35.0	35.0	37.0	32.0	37.0
6	34.017342590434225	35.0	33.0	37.0	28.0	37.0
7	33.9922176972733	35.0	33.0	37.0	30.0	37.0
8	33.875905180228564	35.0	35.0	37.0	28.0	37.0
9	35.11440807726236	37.0	34.0	39.0	29.0	39.0
10	35.12236558844904	37.0	34.0	39.0	28.0	39.0
11	35.537592879528184	37.0	34.0	39.0	30.0	39.0
12	35.65614349420517	37.0	35.0	39.0	30.0	39.0
13	35.865048349917195	37.0	35.0	39.0	30.0	39.0
14	36.62073767332306	38.0	35.0	41.0	30.0	41.0
15	36.9880416417185	39.0	36.0	41.0	31.0	41.0
16	36.95433153401863	39.0	35.0	41.0	31.0	41.0
17	36.142659298770035	38.0	34.0	40.0	29.0	41.0
18	35.819411878524164	38.0	34.0	39.0	29.0	40.0
19	35.04962513007324	37.0	34.0	38.0	27.0	40.0
20	34.09974084383441	35.0	33.0	37.0	27.0	40.0
21	34.2766145459587	35.0	33.0	38.0	29.0	40.0
22	34.61678862699033	35.0	33.0	38.0	29.0	40.0
23	34.95214828425211	35.0	33.0	39.0	30.0	40.0
24	34.29196585415648	35.0	33.0	38.0	27.0	40.0
25	33.21021663383412	35.0	33.0	38.0	23.0	40.0
26	33.191894399575844	35.0	32.0	38.0	23.0	40.0
27	34.102405535977915	35.0	33.0	38.0	25.0	40.0
28	33.86327645914367	35.0	33.0	38.0	24.0	40.0
29	33.68333737074567	35.0	33.0	39.0	23.0	40.0
30	32.82622520228959	35.0	32.0	38.0	21.0	40.0
31	32.67266184310159	35.0	32.0	38.0	20.0	40.0
32	31.67518789202893	35.0	31.0	38.0	15.0	40.0
33	30.608908816946773	35.0	25.0	39.0	9.0	40.0
34	29.557938390611262	35.0	21.0	39.0	7.0	40.0
35	28.81973487151126	35.0	18.0	39.0	7.0	40.0
36	28.00730695455702	35.0	15.0	39.0	7.0	40.0
37	27.705876796458046	35.0	15.0	39.0	7.0	40.0
38	27.53353718456764	34.0	15.0	39.0	7.0	40.0
39	27.117261689832272	34.0	12.0	38.0	7.0	40.0
40	27.014258921538605	33.0	12.0	39.0	7.0	40.0
41	26.766707650210783	33.0	11.0	38.0	7.0	40.0
42	26.662449475212572	33.0	10.0	38.0	7.0	40.0
43	26.60594855574905	33.0	10.0	38.0	7.0	40.0
44	26.565304763689493	33.0	10.0	38.0	7.0	40.0
45	26.68939958346093	33.0	10.0	39.0	7.0	40.0
46	26.45192660746144	33.0	10.0	38.0	7.0	40.0
47	26.324594240059966	33.0	10.0	38.0	7.0	40.0
48	26.164145950170713	33.0	10.0	38.0	7.0	40.0
49	26.268070467319053	33.0	10.0	38.0	7.0	40.0
50	26.231203560235908	33.0	10.0	38.0	7.0	40.0
51	25.273070764411656	31.0	10.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	18.0
10	32.0
11	24.0
12	68.0
13	84.0
14	117.0
15	208.0
16	418.0
17	844.0
18	1750.0
19	3150.0
20	5215.0
21	8020.0
22	11740.0
23	17082.0
24	25223.0
25	36935.0
26	45971.0
27	44871.0
28	36536.0
29	30110.0
30	27919.0
31	28527.0
32	30845.0
33	35470.0
34	41570.0
35	49295.0
36	56156.0
37	61493.0
38	49109.0
39	7555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.588881728195307	2.1066151096728785	29.01863456238259	47.28586859974923
2	47.43730965124375	12.786713409236686	27.960070753746795	11.81590618577277
3	9.44602132058425	12.95278055825986	65.02549663980645	12.57570148134944
4	8.182387436182223	2.2274327694668026	75.87135737802825	13.718822416322723
5	16.231768797963316	2.719387654050134	67.21179350997393	13.837050038012618
6	10.966526042833136	10.809447849582774	69.39230088320299	8.831725224381096
7	45.02918363522513	0.7544628641860196	52.3303486953064	1.886004805282459
8	44.81801935215529	7.55331288726783	45.29321516665372	2.3354525939231614
9	42.19263484576323	2.1808120835942417	44.445053865174806	11.181499205467722
10	23.525163743732488	15.784911047426645	50.49766820393046	10.192257004910408
11	16.676341220761138	14.845946057124051	56.858344721883235	11.61936800023158
12	12.584690437122253	13.638074169549988	58.96526454192129	14.811970851406468
13	12.996201785298028	14.010734946165297	63.31835681888472	9.674706449651945
14	12.498000338228506	18.30197101899717	56.68222213080911	12.517806511965215
15	10.10739516820774	14.873522345172855	63.21780239837529	11.801280088244122
16	11.22141626330632	16.494276777566004	55.98001709425149	16.30428986487619
17	11.615102055119058	16.605343705674162	59.373424076080084	12.406130163126694
18	12.013358502409497	15.538705072361095	58.19708971130216	14.250846713927245
19	11.740033304842914	17.5053057692337	55.89545996791399	14.859200958009389
20	13.421272744724321	16.6707040790053	59.95328790101788	9.95473527525249
21	13.459209185189247	19.60156072649045	56.66470128481126	10.274528803509044
22	12.53228025431127	13.562658354167906	58.64013858227408	15.264922809246743
23	12.46372042214574	18.915505339287375	57.54729485755552	11.073479381011364
24	13.393391746310337	15.979316260411572	56.36166682663961	14.265625166638483
25	10.858506218376778	22.493414447232542	54.73146637292587	11.916612961464804
26	12.202431284003772	15.775007960558291	57.61478820344293	14.407772551995016
27	14.075333543583485	16.97236734053364	54.86858603725694	14.083713078625939
28	11.28753841255041	17.255747980151167	57.97556527581621	13.481148331482217
29	15.982515719245963	15.126279593089778	54.80109269136953	14.090111996294722
30	11.824742786362991	19.2863378537116	55.17466759908039	13.714251760845023
31	14.198588886298852	19.12651726717462	51.92858807881638	14.746305767710147
32	14.502689830748627	23.003499598544096	50.09590758744045	12.39790298326683
33	13.71135701237581	21.449019670577623	46.82408004131873	18.01554327572784
34	19.655037395579566	21.872110012630245	44.80308854426146	13.669764047528723
35	14.17146966379782	24.81789746800922	42.37393751304541	18.63669535514755
36	19.8601074713458	24.462300471844	39.87378896674238	15.803803090067813
37	15.898720368821426	25.742084005600574	41.55380956516307	16.80538606041492
38	17.21704976377329	27.977134534196885	39.13060038606803	15.675215315961797
39	21.072550014397564	25.026167002609846	38.53397749104532	15.367305491947267
40	17.44040246145033	27.773435655073957	39.87135128382094	14.914810599654762
41	21.541803976774975	25.765699058902037	37.539250503914765	15.153246460408221
42	16.99125938317481	26.670993553852224	40.48275263155489	15.854994431418076
43	21.2622322167222	23.57498388843944	37.58861358307395	17.57417031176441
44	19.16719610092617	24.408062026841936	36.78433057418098	19.64041129805092
45	16.841189528323593	24.677883055208945	37.499028735710986	20.981898680756476
46	20.77484798761657	25.495878030535025	36.002748487493925	17.72652549435448
47	16.272599986897454	24.027478780731943	41.84541738464046	17.854503847730136
48	18.91108703899226	25.029366461444237	35.599311963995426	20.460234535568077
49	16.73149379685874	24.03235414657483	39.97388632170406	19.262265734862368
50	18.496376231982094	22.83423299068653	38.40432323066118	20.265067546670203
51	18.090349670379563	22.827072297104795	36.35027065898187	22.73230737353377
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55.0
1	987.0
2	1919.0
3	25915.5
4	49912.0
5	38308.0
6	26704.0
7	25837.0
8	24970.0
9	24682.0
10	24394.0
11	23624.5
12	22855.0
13	22270.5
14	21686.0
15	20577.0
16	19468.0
17	18386.5
18	17305.0
19	16204.0
20	15103.0
21	14064.0
22	13025.0
23	12107.5
24	11190.0
25	11026.0
26	10738.5
27	10615.0
28	10812.5
29	11010.0
30	11672.0
31	12334.0
32	13078.0
33	13822.0
34	14889.5
35	15957.0
36	16822.5
37	17688.0
38	18519.0
39	19350.0
40	20183.0
41	21016.0
42	21660.5
43	22305.0
44	23958.5
45	25612.0
46	51236.0
47	76860.0
48	55955.5
49	35051.0
50	31919.5
51	28788.0
52	25528.5
53	22269.0
54	20203.0
55	18137.0
56	16286.0
57	14435.0
58	12705.0
59	10975.0
60	9354.5
61	7734.0
62	6599.0
63	5464.0
64	4287.0
65	3110.0
66	2466.5
67	1823.0
68	1394.5
69	966.0
70	750.0
71	534.0
72	441.5
73	349.0
74	310.0
75	264.5
76	258.0
77	185.5
78	113.0
79	70.5
80	28.0
81	24.0
82	20.0
83	17.5
84	15.0
85	8.0
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	656361.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.840392963194724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.09769277664128	49.483412902755944
2	7.822764202674125	9.205890394875164
3	3.363286000590692	5.936912097671036
4	1.776908233322488	4.1821591483292515
5	0.9957700441473625	2.9295750349304286
6	0.6013025428647529	2.122852674715818
7	0.35606079678369873	1.4665530041078734
8	0.2324225896428301	1.094066920648598
9	0.16106905916850195	0.8529630062118043
>10	0.5175728279941592	5.121966868207814
>50	0.04018488535777085	1.6585236504118286
>100	0.0297472287507989	3.130342453806219
>500	0.0023484654276907766	0.8785471611076018
>1k	0.0018265842215372705	2.36265007254872
>5k	0.0	0.0
>10k+	0.0010437624123070118	9.573584609671844
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25400	3.8698216377877417	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC	14194	2.1625294616834334	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCG	12323	1.877472915057415	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC	10436	1.5899786855099556	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	3481	0.5303483905960287	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAAGAGT	2401	0.36580479339875466	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTC	2377	0.362148269016593	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	2156	0.32847777366418784	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT	1955	0.29785438196358405	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTT	1542	0.23493169155388577	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTA	1476	0.22487624950294122	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGT	881	0.13422491586185042	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	772	0.11761820095953293	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	730	0.11121928329075007	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5235518259006857E-4	0.0	0.0	0.042050030394858925	0.0
2	1.5235518259006857E-4	0.0	0.0	0.43360284965133516	0.0
3	1.5235518259006857E-4	0.0	0.0	0.5093233753985993	0.0
4	1.5235518259006857E-4	0.0	0.0	1.1629271087099935	0.0
5	1.5235518259006857E-4	0.0	0.0	3.7514416609152583	0.0
6	1.5235518259006857E-4	0.0	0.0	4.124864213443517	0.0
7	1.5235518259006857E-4	0.0	0.0	4.566998953319896	0.0
8	1.5235518259006857E-4	0.0	0.0	5.091710202160092	0.0
9	1.5235518259006857E-4	0.0	0.0	5.212223151588836	0.0
10	1.5235518259006857E-4	0.0	0.0	7.583783923785844	0.0
11	1.5235518259006857E-4	0.0	0.0	7.739795630758073	0.0
12	1.5235518259006857E-4	0.0	0.0	9.765967204023395	0.0
13	1.5235518259006857E-4	0.0	0.0	9.92837782866441	0.0
14	1.5235518259006857E-4	0.0	0.0	10.047976646997613	0.0
15	1.5235518259006857E-4	0.0	0.0	10.376454420661801	0.0
16	1.5235518259006857E-4	0.0	0.0	10.586552217453505	0.0
17	1.5235518259006857E-4	0.0	0.0	10.70889342907333	0.0
18	1.5235518259006857E-4	0.0	0.0	10.785375730733545	0.0
19	1.5235518259006857E-4	0.0	0.0	11.32974079812786	0.0
20	1.5235518259006857E-4	0.0	0.0	11.446292512809261	0.0
21	1.5235518259006857E-4	0.0	0.0	11.52749782512977	0.0
22	1.5235518259006857E-4	0.0	0.0	11.701182733282447	0.0
23	1.5235518259006857E-4	0.0	0.0	11.790919935827997	0.0
24	1.5235518259006857E-4	0.0	0.0	11.864507489019001	0.0
25	1.5235518259006857E-4	0.0	0.0	11.93169612454122	0.0
26	1.5235518259006857E-4	0.0	0.0	12.018538578617559	0.0
27	1.5235518259006857E-4	0.0	0.0	12.138137396950764	0.0
28	1.5235518259006857E-4	0.0	0.0	12.21995213000163	0.0
29	1.5235518259006857E-4	0.0	0.0	12.302376283782857	0.0
30	1.5235518259006857E-4	0.0	0.0	12.39714120735388	0.0
31	1.5235518259006857E-4	0.0	0.0	12.48367895106504	0.0
32	1.5235518259006857E-4	0.0	0.0	12.584842792304844	0.0
33	1.5235518259006857E-4	0.0	0.0	12.680217136606228	0.0
34	1.5235518259006857E-4	0.0	0.0	12.812918500642176	0.0
35	1.5235518259006857E-4	0.0	0.0	12.926118401306598	0.0
36	1.5235518259006857E-4	0.0	0.0	13.031852898024106	0.0
37	1.5235518259006857E-4	0.0	0.0	13.1516040715399	0.0
38	1.5235518259006857E-4	0.0	0.0	13.278515938637426	0.0
39	1.5235518259006857E-4	0.0	0.0	13.414416761507768	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGG	30	2.1640735E-6	45.000004	28
ACCGGTG	35	1.2108649E-7	45.0	17
AGCGGAC	20	7.031073E-4	45.0	37
ACGTGTT	20	7.031073E-4	45.0	41
CGGTGAA	35	1.2108649E-7	45.0	19
CGTTTTT	24245	0.0	44.54527	1
CCGATGA	400	0.0	43.3125	18
CGGCTGT	1255	0.0	41.95219	9
CGATGAA	430	0.0	41.860466	19
ACACGAC	70	0.0	41.785713	26
AGACACG	70	0.0	41.785713	24
ACGGCTG	1265	0.0	41.620552	8
ATACCTG	1415	0.0	41.342754	6
GATACCT	1405	0.0	41.156586	5
TACGGCT	1285	0.0	41.14786	7
CGCTTTT	740	0.0	41.047295	1
CACCGAG	55	6.002665E-11	40.909092	16
TGATACC	1425	0.0	40.894737	4
TACCTGT	1450	0.0	40.655174	7
GATACCG	50	1.0804797E-9	40.5	27
>>END_MODULE