##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933341.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380859
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.91888336628516	31.0	28.0	31.0	16.0	33.0
2	29.249470276401503	31.0	30.0	33.0	16.0	34.0
3	29.337114260133013	31.0	30.0	33.0	16.0	34.0
4	32.621915196962654	35.0	32.0	37.0	19.0	37.0
5	33.94407116544443	35.0	33.0	37.0	30.0	37.0
6	33.26926762922761	35.0	33.0	37.0	26.0	37.0
7	32.79583782974802	35.0	32.0	37.0	26.0	37.0
8	33.42498930050228	35.0	33.0	37.0	28.0	37.0
9	33.604835385273816	35.0	32.0	39.0	26.0	39.0
10	32.74729755631349	37.0	30.0	39.0	17.0	39.0
11	33.71475532940012	37.0	31.0	39.0	23.0	39.0
12	34.23161852549106	37.0	32.0	39.0	27.0	39.0
13	34.47825048114919	37.0	32.0	39.0	27.0	39.0
14	34.51960436802071	37.0	32.0	40.0	24.0	41.0
15	35.13470601981311	38.0	32.0	40.0	27.0	41.0
16	35.282104400841256	38.0	32.0	40.0	27.0	41.0
17	33.40603215363166	36.0	30.0	40.0	19.0	41.0
18	33.86664093535928	37.0	32.0	39.0	25.0	39.0
19	33.54169653336274	37.0	32.0	37.0	25.0	39.0
20	32.83244980425827	35.0	32.0	36.0	25.0	39.0
21	32.96075713059164	35.0	32.0	37.0	25.0	39.0
22	32.828432569533604	34.0	30.0	37.0	25.0	39.0
23	33.89847423849771	35.0	33.0	38.0	27.0	40.0
24	32.03757558571545	35.0	31.0	36.0	21.0	39.0
25	31.069949771437724	34.0	27.0	36.0	18.0	39.0
26	29.97070831987691	33.0	25.0	36.0	18.0	39.0
27	32.18690118915399	34.0	30.0	37.0	22.0	39.0
28	32.3170989788872	35.0	31.0	37.0	21.0	39.0
29	32.196999414481475	35.0	30.0	38.0	21.0	40.0
30	31.16658133324931	34.0	27.0	37.0	18.0	40.0
31	31.62326215213504	35.0	30.0	37.0	20.0	39.0
32	31.21774987593834	35.0	30.0	37.0	16.0	39.0
33	30.575160361183535	35.0	27.0	38.0	12.0	40.0
34	28.999585148309478	34.0	21.0	37.0	8.0	40.0
35	28.183876447714248	34.0	18.0	37.0	7.0	40.0
36	27.359264189634484	33.0	15.0	37.0	7.0	40.0
37	27.1832384163168	33.0	15.0	37.0	7.0	40.0
38	27.199036913923525	33.0	15.0	38.0	7.0	40.0
39	26.81486849463975	33.0	15.0	37.0	7.0	40.0
40	26.764868363357568	33.0	15.0	37.0	7.0	40.0
41	26.47299394264019	33.0	15.0	37.0	7.0	40.0
42	26.547499205742806	33.0	14.0	37.0	7.0	40.0
43	26.63795000249436	33.0	13.0	37.0	7.0	40.0
44	26.47886488175414	33.0	12.0	37.0	7.0	40.0
45	26.641649534342104	33.0	10.0	38.0	7.0	40.0
46	26.354167815385747	33.0	10.0	38.0	7.0	40.0
47	26.322783497304776	33.0	10.0	38.0	7.0	40.0
48	26.059848920466628	32.0	10.0	37.0	7.0	40.0
49	25.776182261676894	32.0	10.0	37.0	7.0	39.0
50	25.94794136412688	32.0	10.0	37.0	7.0	40.0
51	24.70355170811245	30.0	10.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	13.0
11	15.0
12	15.0
13	43.0
14	83.0
15	175.0
16	438.0
17	768.0
18	1507.0
19	2407.0
20	3990.0
21	5913.0
22	8306.0
23	11811.0
24	16112.0
25	21773.0
26	26351.0
27	25676.0
28	22018.0
29	20185.0
30	20784.0
31	23035.0
32	26186.0
33	29789.0
34	33440.0
35	35987.0
36	26529.0
37	14371.0
38	3076.0
39	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.552272625827406	2.0695322941035923	31.17610454262601	48.20209053744299
2	50.025074896484	9.390876938709601	29.08582966399639	11.49821850081001
3	9.690725439073253	9.398753869542272	68.82153237812419	12.088988313260288
4	8.593468974082272	2.269081208531241	75.32420134485466	13.81324847253183
5	14.494602989557814	2.706775998466624	69.36294009068973	13.435680921285831
6	11.138505326118064	8.127154668788187	70.84327795850957	9.89106204658417
7	45.25112968316359	0.724677636605673	51.80237305669553	2.221819623535219
8	46.65033516340693	5.640145040553065	45.135863928645506	2.5736558673944954
9	43.325745223297865	2.084235898324577	44.948392974827954	9.641625903549608
10	22.432448754000823	16.774974465615884	50.51370717247066	10.27886960791264
11	15.227682685718335	15.333233558876119	58.19712807101841	11.241955684387134
12	12.555302618554373	13.809310007115494	59.48316831163238	14.152219062697743
13	12.604402154078018	14.413995730703489	62.95952045245091	10.022081662767587
14	12.222371008693505	16.26586216946429	58.122297228108046	13.389469593734162
15	10.003964721852444	15.18462213049974	62.743692547635746	12.067720600012077
16	10.841282469365304	15.46845420483696	57.425713978138894	16.264549347658843
17	11.305233695409587	16.006973709430525	60.03376577683605	12.65402681832384
18	11.479313866811601	15.03075941490158	58.99532372872901	14.494602989557814
19	11.515547748641886	17.580259361075885	56.58288237904316	14.32131051123907
20	13.011114349404899	16.549169115079334	59.93267849781678	10.507038037698992
21	13.278404868993512	18.866299601689864	56.73648253028023	11.118812999036388
22	12.174059166253128	14.65240417057231	58.54345046329481	14.630086199879747
23	11.93696354818975	18.90489656276995	57.266599975318954	11.89153991372135
24	12.235236662386868	16.046620927954965	57.585090545319915	14.133051864338245
25	11.011161610989893	20.08722388075377	55.63975119401143	13.261863314244904
26	11.88628862649957	16.78573960442053	57.6764629429789	13.651508826100997
27	12.317419307407729	17.320058079236674	56.000514626147734	14.362007987207864
28	11.167912534560035	17.216607720967602	58.470720135273154	13.144759609199205
29	14.362007987207864	15.233984230384474	55.81540675157998	14.58860103082768
30	11.874998358972743	18.40497401925647	56.243649224516155	13.476378397254626
31	13.330917741211316	18.605835755489565	53.7143667341457	14.348879769153413
32	13.522064596084116	21.56231046135184	52.315948946985635	12.599675995578416
33	12.73200843356728	20.57323051312953	49.81371058580735	16.881050467495847
34	17.417994585922873	21.803344544831553	46.87377743469368	13.904883434551895
35	13.423340396314645	23.877865561795836	44.25574819027514	18.443045851614375
36	17.79372418664125	23.813799857690114	42.30778319535576	16.084692760312873
37	15.18435956613865	25.2980761909263	43.07945985259637	16.43810439033868
38	16.186305168054318	26.756358652414676	42.329838601687236	14.727497577843767
39	19.216823023743697	24.539265187379055	41.32395453435523	14.919957254522014
40	17.12129685789229	26.172152948991624	42.19855642114273	14.507993771973354
41	20.321956419567346	23.962148721705407	40.925381834222115	14.790513024505131
42	16.532102431608546	25.27602078459482	42.721847192793135	15.470029591003495
43	19.69127682423154	22.889310742295706	40.82219404031413	16.597218393158624
44	18.09383525136599	23.654948419231264	39.94050291577723	18.310713413625514
45	16.104910216116725	24.01466159392321	40.05996970007273	19.820458489887333
46	18.78569234283554	24.923134283291194	38.222281736810736	18.068891637062535
47	15.664589782570454	23.548347288629127	42.75493030229035	18.032132626510073
48	17.794774444085608	23.816162936939918	38.24249919261459	20.146563426359887
49	15.73206882337033	23.416277415001353	40.85527714981135	19.99637661181697
50	17.429547417810788	21.874236922325586	40.582735343000955	20.11348031686267
51	17.075873223423894	21.89944310099013	39.10712363368071	21.917560041905272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47.0
1	258.5
2	470.0
3	19635.5
4	38801.0
5	27133.5
6	15466.0
7	14963.5
8	14461.0
9	14399.5
10	14338.0
11	13947.5
12	13557.0
13	12877.5
14	12198.0
15	11368.5
16	10539.0
17	9939.5
18	9340.0
19	8678.5
20	8017.0
21	7428.5
22	6840.0
23	6393.5
24	5947.0
25	5813.5
26	5616.0
27	5552.0
28	5744.0
29	5936.0
30	6433.0
31	6930.0
32	7285.5
33	7641.0
34	8416.5
35	9192.0
36	9874.5
37	10557.0
38	11145.0
39	11733.0
40	12101.0
41	12469.0
42	13173.5
43	13878.0
44	14633.5
45	15389.0
46	24756.5
47	34124.0
48	28724.5
49	23325.0
50	21354.5
51	19384.0
52	16744.5
53	14105.0
54	12507.0
55	10909.0
56	9392.0
57	7875.0
58	6740.5
59	5606.0
60	4736.5
61	3867.0
62	3309.5
63	2752.0
64	2157.5
65	1563.0
66	1216.5
67	870.0
68	675.0
69	480.0
70	373.0
71	266.0
72	220.0
73	174.0
74	183.5
75	223.5
76	254.0
77	166.0
78	78.0
79	56.0
80	34.0
81	22.0
82	10.0
83	8.0
84	6.0
85	4.5
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	380859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.50474742272878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.74038161942677	51.27195386389065
2	8.282298640859596	10.022367746896395
3	3.0919224695272023	5.612279642082096
4	1.4914417919182057	3.609572356628522
5	0.787104012389053	2.381176473250802
6	0.4799342615319995	1.7422980764104492
7	0.2776035048954932	1.1757430963156246
8	0.17537790440969706	0.8488956647868954
9	0.12105459562015196	0.6591939959122077
>10	0.47506168056196924	5.139270684701066
>50	0.04044856347516811	1.7163232513911768
>100	0.0338536889954956	4.165781481210541
>500	8.793165972856E-4	0.4562142554454711
>1k	0.0017586331945712	2.7497881973993206
>5k	4.396582986428E-4	1.3542780026081012
>10k+	4.396582986428E-4	7.094863211070647
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26671	7.002854074605038	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCG	5091	1.3367151623041598	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC	4202	1.1032954452960282	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC	3946	1.0360789688572412	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	1155	0.3032618370578088	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAAGAGT	1034	0.27149154936603836	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	979	0.25705050950614267	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTC	736	0.1932473697615128	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTT	498	0.13075705182232794	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	497	0.13049448746123893	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT	476	0.12498063587836968	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTA	432	0.11342780399045316	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGCAAAGAGTCGTATGCCG	426	0.11185241782391908	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	422	0.11080216037956303	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC	409	0.10738882368540588	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	383	0.10056215029709158	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6256436108901195E-4	0.0	0.0	0.07955700140997062	0.0
2	2.6256436108901195E-4	0.0	0.0	0.4655266122108182	0.0
3	2.6256436108901195E-4	0.0	0.0	0.5952334065887901	0.0
4	2.6256436108901195E-4	0.0	0.0	1.1689365355682813	0.0
5	2.6256436108901195E-4	0.0	0.0	2.6049010263640877	0.0
6	2.6256436108901195E-4	0.0	0.0	3.128454362375577	0.0
7	2.6256436108901195E-4	0.0	0.0	3.60868457880738	0.0
8	2.6256436108901195E-4	0.0	0.0	4.171360004621133	0.0
9	2.6256436108901195E-4	0.0	0.0	4.461493623624491	0.0
10	2.6256436108901195E-4	0.0	0.0	6.490328441759286	0.0
11	2.6256436108901195E-4	0.0	0.0	6.9453524795265436	0.0
12	2.6256436108901195E-4	0.0	0.0	8.594781795887718	0.0
13	2.6256436108901195E-4	0.0	0.0	8.810872265063974	0.0
14	2.6256436108901195E-4	0.0	0.0	8.952394455690952	0.0
15	2.6256436108901195E-4	0.0	0.0	9.216796767307587	0.0
16	5.251287221780239E-4	0.0	0.0	9.409519008346921	0.0
17	5.251287221780239E-4	0.0	0.0	9.592788932387052	0.0
18	5.251287221780239E-4	0.0	0.0	9.785773737787475	0.0
19	5.251287221780239E-4	0.0	0.0	10.28175781588462	0.0
20	5.251287221780239E-4	0.0	0.0	10.443234897954362	0.0
21	5.251287221780239E-4	0.0	0.0	10.635694574632607	0.0
22	5.251287221780239E-4	0.0	0.0	10.885655846389346	0.0
23	5.251287221780239E-4	0.0	0.0	11.100958622482336	0.0
24	5.251287221780239E-4	0.0	0.0	11.267949556134948	0.0
25	5.251287221780239E-4	0.0	0.0	11.402119944651433	0.0
26	5.251287221780239E-4	0.0	0.0	11.541804184750786	0.0
27	5.251287221780239E-4	0.0	0.0	11.695929464710037	0.0
28	5.251287221780239E-4	0.0	0.0	11.834038318642857	0.0
29	5.251287221780239E-4	0.0	0.0	11.978973845963992	0.0
30	5.251287221780239E-4	0.0	0.0	12.156204789699075	0.0
31	5.251287221780239E-4	0.0	0.0	12.323720852073865	0.0
32	5.251287221780239E-4	0.0	0.0	12.491236914448654	0.0
33	5.251287221780239E-4	0.0	0.0	12.661903749156512	0.0
34	5.251287221780239E-4	0.0	0.0	12.84044751469704	0.0
35	5.251287221780239E-4	0.0	0.0	13.023454874376082	0.0
36	5.251287221780239E-4	0.0	0.0	13.198060174500274	0.0
37	5.251287221780239E-4	0.0	0.0	13.378179326207336	0.0
38	5.251287221780239E-4	0.0	0.0	13.559348735358755	0.0
39	5.251287221780239E-4	0.0	0.0	13.771500739118677	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	25	3.8858772E-5	45.0	6
CGCGGGT	20	7.02734E-4	45.0	4
ACCGCAG	45	3.8380676E-10	45.0	35
GATCCGT	20	7.02734E-4	45.0	5
GATCCGG	40	6.7939254E-9	45.0	5
ATCCGTC	20	7.02734E-4	45.0	6
ATCCGGC	35	1.209064E-7	45.0	6
CCCGTTG	20	7.02734E-4	45.0	1
GGTATGC	40	6.7939254E-9	45.0	8
TCGTAGG	20	7.02734E-4	45.0	2
ACCCGTC	35	1.209064E-7	45.0	17
GGACCTA	25	3.8858772E-5	45.0	8
TATACGG	20	7.02734E-4	45.0	27
CCGCCTC	20	7.02734E-4	45.0	15
TGATCCG	60	0.0	44.999996	4
CCCCGCT	30	2.1616652E-6	44.999996	16
CGTTTTT	15750	0.0	44.42857	1
CGATGAA	115	0.0	43.043476	19
CGACCAC	440	0.0	42.954548	12
ACCACCG	260	0.0	42.403843	14
>>END_MODULE