##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933340.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	843000
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.569370106761564	31.0	28.0	33.0	25.0	34.0
2	28.918517200474497	31.0	28.0	33.0	16.0	34.0
3	28.9415931198102	31.0	28.0	34.0	16.0	34.0
4	33.25896559905101	35.0	32.0	37.0	28.0	37.0
5	34.298086595492286	35.0	33.0	37.0	30.0	37.0
6	33.83962040332147	35.0	33.0	37.0	28.0	37.0
7	33.60300711743772	35.0	32.0	37.0	29.0	37.0
8	33.690479240806646	35.0	33.0	37.0	28.0	37.0
9	34.74277580071174	37.0	34.0	39.0	28.0	39.0
10	34.65198220640569	37.0	33.0	39.0	27.0	39.0
11	34.922162514827995	37.0	34.0	39.0	27.0	39.0
12	35.068979833926456	37.0	34.0	39.0	27.0	39.0
13	35.29776037959668	37.0	34.0	39.0	30.0	39.0
14	35.97872597864769	38.0	34.0	40.0	27.0	41.0
15	36.35887425860024	38.0	34.0	40.0	30.0	41.0
16	36.29094661921708	38.0	34.0	40.0	30.0	41.0
17	35.57695729537367	38.0	33.0	40.0	27.0	41.0
18	35.321161328588374	37.0	33.0	39.0	27.0	40.0
19	34.623894424673786	37.0	33.0	38.0	27.0	40.0
20	34.20189916963226	35.0	33.0	38.0	27.0	40.0
21	34.43981257413998	35.0	33.0	38.0	27.0	40.0
22	35.023462633451956	35.0	33.0	39.0	30.0	40.0
23	35.38070106761566	36.0	34.0	39.0	30.0	40.0
24	34.81508185053381	35.0	33.0	39.0	28.0	40.0
25	33.700625148279954	35.0	33.0	38.0	24.0	40.0
26	33.82915065243179	35.0	33.0	38.0	25.0	40.0
27	34.614640569395014	35.0	33.0	39.0	27.0	40.0
28	34.37177461447212	35.0	33.0	39.0	25.0	40.0
29	34.495725978647684	35.0	33.0	39.0	25.0	40.0
30	33.68793712930012	35.0	33.0	39.0	22.0	40.0
31	33.70840925266904	35.0	33.0	39.0	23.0	40.0
32	33.094365361803085	35.0	33.0	39.0	18.0	40.0
33	32.51082799525504	35.0	31.0	39.0	15.0	40.0
34	31.9847034400949	36.0	31.0	40.0	10.0	41.0
35	31.527360616844604	36.0	30.0	40.0	8.0	41.0
36	31.051835112692764	36.0	27.0	40.0	8.0	41.0
37	30.940837485172004	36.0	26.0	40.0	7.0	41.0
38	30.64782206405694	35.0	25.0	40.0	8.0	41.0
39	30.237876631079477	35.0	23.0	39.0	7.0	40.0
40	29.935285883748517	35.0	23.0	39.0	7.0	40.0
41	29.6819478054567	35.0	22.0	39.0	7.0	40.0
42	29.773848161328587	35.0	22.0	39.0	7.0	40.0
43	29.672166073546858	35.0	22.0	39.0	7.0	40.0
44	29.773328588374852	35.0	22.0	39.0	7.0	40.0
45	29.869469750889678	35.0	23.0	39.0	7.0	40.0
46	29.600724792408066	35.0	22.0	39.0	7.0	40.0
47	29.477198102016608	35.0	22.0	39.0	7.0	40.0
48	29.364727164887306	35.0	22.0	39.0	7.0	40.0
49	29.53272953736655	35.0	22.0	39.0	7.0	40.0
50	29.538188612099646	35.0	22.0	39.0	7.0	40.0
51	28.254542111506524	34.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	30.0
10	25.0
11	51.0
12	54.0
13	93.0
14	132.0
15	207.0
16	380.0
17	737.0
18	1533.0
19	2776.0
20	4453.0
21	6870.0
22	9883.0
23	14438.0
24	21142.0
25	30800.0
26	40120.0
27	42728.0
28	39874.0
29	37246.0
30	37345.0
31	40297.0
32	46127.0
33	54956.0
34	64046.0
35	75977.0
36	86737.0
37	94025.0
38	77799.0
39	12105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.203795966785293	2.5812574139976276	34.355397390272834	37.859549228944246
2	36.822419928825624	14.056227758007116	35.1361803084223	13.985172004744959
3	12.71043890865955	14.240688018979833	58.744365361803084	14.304507710557532
4	11.099525504151838	2.8123368920521945	69.32882562277581	16.75931198102017
5	19.513879003558717	3.412811387900356	60.02609727164887	17.047212336892052
6	14.069039145907473	12.246856465005932	62.179240806642945	11.504863582443653
7	56.0926453143535	1.0207591933570581	40.51874258600237	2.36785290628707
8	55.6858837485172	8.56120996441281	33.00118623962041	2.751720047449585
9	52.73831553973902	2.8220640569395017	32.099051008303675	12.340569395017793
10	29.526215895610914	22.268446026097273	37.50296559905101	10.702372479240807
11	21.667141162514827	17.31838671411625	47.37342823250297	13.641043890865955
12	16.952431791221827	16.64733096085409	49.19988137603796	17.20035587188612
13	16.62835112692764	16.909489916963228	53.89845788849348	12.563701067615659
14	15.045077105575327	21.746737841043892	47.235112692763934	15.973072360616843
15	12.627164887307234	16.799051008303678	54.870937129300124	15.702846975088969
16	14.980427046263344	19.192052194543297	46.63214709371293	19.195373665480428
17	15.37876631079478	18.702016607354686	50.379359430604985	15.539857651245551
18	15.946500593119811	17.26334519572954	48.948635824436536	17.841518386714117
19	15.23962040332147	19.04258600237248	46.74246737841044	18.97532621589561
20	17.66405693950178	19.345195729537366	50.42218268090154	12.568564650059312
21	17.14650059311981	22.584223013048636	47.42325029655991	12.846026097271649
22	16.158956109134046	15.4570581257414	49.81126927639383	18.572716488730723
23	15.527639383155398	21.909134045077106	48.75421115065243	13.809015421115065
24	16.75634638196916	18.173309608540926	47.5785290628707	17.491814946619215
25	14.267852906287072	25.373428232502963	45.12882562277581	15.229893238434164
26	15.38612099644128	17.511862396204034	48.79857651245552	18.30344009489917
27	17.213997627520758	19.15409252669039	45.704982206405695	17.926927639383155
28	12.557532621589562	20.06773428232503	49.970581257414	17.40415183867141
29	16.769276393831554	17.45314353499407	48.56465005931198	17.212930011862397
30	14.741162514827996	20.09632265717675	47.866310794780546	17.29620403321471
31	18.695610913404508	17.619691577698696	43.8141162514828	19.870581257413996
32	20.397627520759194	21.72550415183867	43.119810201660734	14.757058125741398
33	16.626097271648874	22.218861209964412	39.90782918149466	21.24721233689205
34	20.6938315539739	21.83831553973903	42.445432977461444	15.022419928825622
35	16.203084223013047	26.22787663107948	38.0570581257414	19.511981020166075
36	17.050889679715304	26.315539739027283	37.52775800711744	19.10581257413998
37	19.901897983392644	23.602372479240806	37.15836298932385	19.337366548042706
38	18.40011862396204	27.166073546856467	34.309371293001185	20.12443653618031
39	24.593950177935945	23.27425860023725	34.801660735468566	17.330130486358243
40	19.722419928825623	27.45290628706999	36.18659549228944	16.63807829181495
41	20.179952550415184	27.851957295373666	33.491340450771055	18.476749703440095
42	19.493950177935943	25.923013048635823	37.28161328588374	17.301423487544483
43	20.813048635824437	25.202372479240807	34.14033214709371	19.844246737841043
44	21.395136417556344	23.5202846975089	34.0202846975089	21.064294187425862
45	18.851008303677343	22.759786476868328	34.754092526690386	23.635112692763936
46	22.716251482799528	25.050533807829183	33.207591933570576	19.025622775800713
47	16.487069988137605	24.440688018979834	40.2373665480427	18.834875444839856
48	19.708422301304864	25.21850533807829	33.631198102016604	21.441874258600237
49	18.457651245551602	22.994780545670228	38.63013048635825	19.91743772241993
50	18.555397390272834	23.40094899169632	36.92692763938316	21.116725978647686
51	18.367497034400948	21.898695136417558	35.13760379596679	24.596204033214708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	1181.5
2	2293.0
3	26688.0
4	51083.0
5	38485.5
6	25888.0
7	24555.0
8	23222.0
9	22376.0
10	21530.0
11	20782.5
12	20035.0
13	18682.0
14	17329.0
15	16409.5
16	15490.0
17	14193.0
18	12896.0
19	12116.0
20	11336.0
21	10616.5
22	9897.0
23	9695.0
24	9493.0
25	9481.0
26	10014.0
27	10559.0
28	11576.0
29	12593.0
30	14419.0
31	16245.0
32	17466.0
33	18687.0
34	20739.5
35	22792.0
36	24413.0
37	26034.0
38	27096.5
39	28159.0
40	28973.0
41	29787.0
42	31252.0
43	32717.0
44	34649.5
45	36582.0
46	73417.5
47	110253.0
48	80264.0
49	50275.0
50	46528.5
51	42782.0
52	39650.5
53	36519.0
54	34914.5
55	33310.0
56	30710.0
57	28110.0
58	25818.5
59	23527.0
60	20766.0
61	18005.0
62	15289.5
63	12574.0
64	10586.0
65	8598.0
66	6955.5
67	5313.0
68	4318.0
69	3323.0
70	2748.0
71	2173.0
72	1775.0
73	1377.0
74	1198.0
75	919.5
76	820.0
77	605.5
78	391.0
79	310.0
80	229.0
81	185.5
82	142.0
83	95.5
84	49.0
85	32.5
86	16.0
87	10.0
88	4.0
89	4.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	843000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.95446974859692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.40077415492311	35.39481560698612
2	5.513362723783165	4.736471446635718
3	2.4315199390775555	3.1333394899865126
4	1.6252981636002144	2.792552832032621
5	1.2498775215872173	2.684391309523471
6	1.0050594554007082	2.590307758353061
7	0.8526477982906927	2.5637523840519725
8	0.7580996820068935	2.6051015885748843
9	0.6147408201000816	2.3765279364194956
>10	3.4256541550383455	22.86017805710807
>50	0.07114641951081338	2.108076890768738
>100	0.045656887400197674	3.583522055418627
>500	0.0028010360368067714	0.7792933236930726
>1k	0.002240828829445417	2.84814444317777
>5k	0.0	0.0
>10k+	0.0011204144147227086	8.943524877269905
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23286	2.7622775800711743	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	18779	2.2276393831553976	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	16908	2.005693950177936	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	15360	1.8220640569395017	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	4927	0.5844602609727164	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	3682	0.436773428232503	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	3400	0.4033214709371293	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	3066	0.3637010676156584	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	3001	0.3559905100830368	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	2810	0.33333333333333337	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	1449	0.17188612099644127	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGT	1337	0.15860023724792408	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05219454329774614	0.0
2	0.0	0.0	0.0	0.4889679715302491	0.0
3	0.0	0.0	0.0	0.560023724792408	0.0
4	0.0	0.0	0.0	1.2830367734282324	0.0
5	0.0	0.0	0.0	4.032384341637011	0.0
6	0.0	0.0	0.0	4.304863582443653	0.0
7	0.0	0.0	0.0	4.771055753262159	0.0
8	0.0	0.0	0.0	5.35741399762752	0.0
9	0.0	0.0	0.0	5.450771055753262	0.0
10	0.0	0.0	0.0	7.948042704626334	0.0
11	0.0	0.0	0.0	8.11779359430605	0.0
12	0.0	0.0	0.0	10.4570581257414	0.0
13	0.0	0.0	0.0	10.659193357058125	0.0
14	0.0	0.0	0.0	10.797746144721234	0.0
15	0.0	0.0	0.0	11.280782918149466	0.0
16	0.0	0.0	0.0	11.529300118623961	0.0
17	0.0	0.0	0.0	11.645077105575327	0.0
18	0.0	0.0	0.0	11.746263345195729	0.0
19	0.0	0.0	0.0	12.343179122182681	0.0
20	0.0	0.0	0.0	12.487307236061685	0.0
21	0.0	0.0	0.0	12.607710557532622	0.0
22	0.0	0.0	0.0	12.836061684460262	0.0
23	0.0	0.0	0.0	12.96073546856465	0.0
24	0.0	0.0	0.0	13.069276393831554	0.0
25	0.0	0.0	0.0	13.155871886120996	0.0
26	0.0	0.0	0.0	13.272479240806643	0.0
27	0.0	0.0	0.0	13.419335705812575	0.0
28	0.0	0.0	0.0	13.520759193357058	0.0
29	0.0	0.0	0.0	13.631553973902728	0.0
30	0.0	0.0	0.0	13.779596678529062	0.0
31	0.0	0.0	0.0	13.904270462633452	0.0
32	0.0	0.0	0.0	14.041518386714117	0.0
33	0.0	0.0	0.0	14.175800711743772	0.0
34	0.0	0.0	0.0	14.344246737841043	0.0
35	0.0	0.0	0.0	14.499762752075918	0.0
36	0.0	0.0	0.0	14.655160142348754	0.0
37	0.0	0.0	0.0	14.81103202846975	0.0
38	0.0	0.0	0.0	14.980308422301304	0.0
39	0.0	0.0	0.0	15.224080664294187	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGAACG	20	7.032216E-4	45.0	17
GCGATCG	35	1.2114106E-7	45.0	9
CGTTTTT	20895	0.0	44.321606	1
TGATACC	1935	0.0	42.09302	4
GATACCT	1965	0.0	41.67939	5
CGGCTGT	2090	0.0	41.44737	9
TACGGCT	2055	0.0	41.16788	7
ACGGCTG	2055	0.0	41.16788	8
GAATCTG	2290	0.0	40.97162	1
CGATGAA	575	0.0	40.695652	19
CGCTTTT	655	0.0	40.53435	1
ATGATAC	6685	0.0	40.186985	3
ATACCTG	2040	0.0	40.14706	6
GCGAGAC	460	0.0	40.1087	21
ACCGGTG	90	0.0	40.0	17
CGACCAC	1530	0.0	39.852943	12
GAATGAT	7080	0.0	39.819916	1
CGAGACA	465	0.0	39.67742	22
GCGACCT	210	0.0	39.642857	11
AATGATA	6690	0.0	39.5852	2
>>END_MODULE