##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933336.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644218
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.287682120027693	31.0	28.0	31.0	25.0	34.0
2	28.30958619597714	31.0	26.0	33.0	16.0	34.0
3	28.295618874356197	31.0	25.0	33.0	16.0	34.0
4	33.000288722140645	35.0	32.0	37.0	28.0	37.0
5	34.16723686702328	35.0	33.0	37.0	30.0	37.0
6	33.51828884011313	35.0	33.0	37.0	28.0	37.0
7	33.60606968448569	35.0	33.0	37.0	29.0	37.0
8	33.46802945586742	35.0	33.0	37.0	28.0	37.0
9	34.7039852969026	37.0	34.0	39.0	28.0	39.0
10	34.54164894492237	37.0	32.0	39.0	27.0	39.0
11	35.03106401870174	37.0	34.0	39.0	28.0	39.0
12	35.14947890310423	37.0	34.0	39.0	30.0	39.0
13	35.38282848352576	37.0	34.0	39.0	30.0	39.0
14	36.14616325529557	38.0	34.0	40.0	30.0	41.0
15	36.417225225001474	38.0	34.0	40.0	30.0	41.0
16	36.374918117780005	38.0	34.0	40.0	30.0	41.0
17	35.59717517983043	38.0	33.0	40.0	27.0	41.0
18	35.38690008661664	37.0	34.0	39.0	27.0	40.0
19	34.7568012691356	37.0	33.0	38.0	27.0	40.0
20	33.80653288172637	35.0	32.0	38.0	27.0	40.0
21	34.237782862323	35.0	33.0	38.0	27.0	40.0
22	34.752357121347124	35.0	33.0	39.0	29.0	40.0
23	35.0010307069967	35.0	33.0	39.0	29.0	40.0
24	34.490139983670126	35.0	33.0	39.0	27.0	40.0
25	33.48805528563312	35.0	32.0	38.0	24.0	40.0
26	33.51125240213717	35.0	32.0	38.0	24.0	40.0
27	34.27742472268704	35.0	33.0	38.0	26.0	40.0
28	33.90711995007901	35.0	33.0	39.0	24.0	40.0
29	33.93171255692948	35.0	33.0	39.0	24.0	40.0
30	33.16504351011614	35.0	32.0	39.0	21.0	40.0
31	33.165714090571825	35.0	33.0	39.0	21.0	40.0
32	32.37649832820567	35.0	31.0	39.0	16.0	40.0
33	31.56085672862292	35.0	30.0	39.0	10.0	40.0
34	30.839970320605758	35.0	25.0	39.0	8.0	40.0
35	30.33380160132129	35.0	23.0	40.0	7.0	41.0
36	29.91603463423872	35.0	21.0	40.0	7.0	40.0
37	29.734776116159438	36.0	20.0	40.0	7.0	40.0
38	29.58274838641578	35.0	20.0	40.0	7.0	40.0
39	29.148980624571184	35.0	18.0	39.0	7.0	40.0
40	29.03029098845422	35.0	18.0	39.0	7.0	40.0
41	28.5376875529712	35.0	18.0	39.0	7.0	40.0
42	28.803599092232755	35.0	18.0	39.0	7.0	40.0
43	28.837519286949448	35.0	18.0	39.0	7.0	40.0
44	28.93862326107001	35.0	18.0	39.0	7.0	40.0
45	29.023648826949884	35.0	18.0	39.0	7.0	40.0
46	28.768084716664234	35.0	18.0	39.0	7.0	40.0
47	28.625609653874932	35.0	18.0	39.0	7.0	40.0
48	28.488829868150223	35.0	18.0	39.0	7.0	40.0
49	28.659627641574748	35.0	18.0	39.0	7.0	40.0
50	28.62184540015957	35.0	17.0	39.0	7.0	40.0
51	27.562629420475677	34.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	26.0
10	24.0
11	29.0
12	63.0
13	67.0
14	114.0
15	219.0
16	313.0
17	739.0
18	1483.0
19	3027.0
20	4714.0
21	7051.0
22	10338.0
23	14963.0
24	21440.0
25	30566.0
26	36599.0
27	34443.0
28	28403.0
29	24677.0
30	25126.0
31	28302.0
32	34126.0
33	40909.0
34	48044.0
35	57702.0
36	64630.0
37	67086.0
38	51441.0
39	7544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.679887864046023	2.3327507148201385	30.929436929734965	38.05792449139888
2	38.47936568056155	19.07149443200904	30.1104595028391	12.33868038459031
3	10.109621277269495	19.22315116932467	57.76817785283863	12.8990497005672
4	9.190367235935662	2.5098646731385963	73.71386704500651	14.58590104591924
5	21.06243538677901	3.015594100133806	59.859550649003914	16.062419864083278
6	11.962565466969256	15.132455162693374	63.49993325240835	9.405046117929023
7	49.92921650745555	0.9090090621497692	46.93535418134855	2.2264202490461305
8	48.427085241331355	11.148089621836087	37.123302981288944	3.30152215554362
9	45.7093716723221	2.1609144730510477	35.48317495009453	16.646538904532314
10	27.99223244305499	13.46919831485615	44.995948576413575	13.542620665675283
11	21.491793150765734	16.315439804538215	47.42742363609834	14.765343408597712
12	14.369669894352533	13.904299476264246	52.46981611814634	19.25621451123688
13	15.34045928552136	15.566159281485458	58.33491147406623	10.758469958926947
14	14.39761074667271	22.45854664104387	48.64843888249009	14.49540372979333
15	11.312319742695795	16.446917037400382	58.027872552458945	14.212890667444873
16	12.049958243948478	18.531770301357614	48.19548662098855	21.22278483370536
17	12.82826620802275	19.38707083626971	52.21772753943541	15.566935416272132
18	13.043100316973447	18.274714460011985	50.80190246158909	17.88028276142548
19	13.177216408110299	20.31377577155559	47.4892039651174	19.019803855216715
20	15.420711622463203	19.195831224833828	53.37587586810676	12.007581284596208
21	15.174676895088309	22.842112452616973	49.58880379002139	12.39440686227333
22	13.653142259297319	14.840007575075518	52.11745092499744	19.389399240629725
23	14.545076356140314	21.731463572889922	50.696348130601756	13.02711194036801
24	16.336395443778347	18.096358686034854	47.929738070032194	17.637507800154605
25	12.31136044009947	26.881738790285276	46.17396595562372	14.632934813991538
26	13.32499247149257	17.243386555482772	50.634102120710686	18.797518852313967
27	17.554771831895412	19.22020185713532	46.525244560071286	16.699781750897987
28	12.200062711690764	19.06683762328901	50.71699331592722	18.016106349093008
29	15.703535138726332	16.138325846219757	47.66274770341717	20.495391311636745
30	12.84549640028686	20.691442958749988	51.208441862847664	15.254618778115484
31	14.90147745017991	17.83355944726785	44.4740755458556	22.790887556696646
32	16.29215576093807	24.565442132942575	42.93717344128851	16.205228664830848
33	13.932706009456428	19.768929151312133	40.370340474808216	25.928024364423223
34	17.668397964664134	24.42014970087765	37.86730578779233	20.044146546665882
35	18.38383901101801	22.862757637942437	39.40467357323142	19.34872977780813
36	16.02190562821902	25.17641543701045	34.298948492591016	24.50273044217951
37	15.779751574777482	26.429096982698404	41.25948048641919	16.53167095610492
38	18.195238257856815	22.93943975486559	33.56301748786901	25.302304499408585
39	19.74160920682129	28.639994536011105	33.86369210422559	17.754704152942015
40	20.052839256276602	22.708462042352124	36.99865573454949	20.24004296682179
41	17.229105675408015	31.171901437091172	33.46149905777237	18.137493829728445
42	20.022414772639074	23.709210236286474	38.837474271131825	17.430900719942628
43	20.111980727020978	24.967014271566455	33.41446528970007	21.506539711712495
44	19.508768770819813	23.58968547913905	34.676615679785414	22.224930070255724
45	17.493922864620362	22.456683917555857	34.75267688887923	25.29671632894455
46	23.519678121381272	25.948359095834018	31.945707819402745	18.58625496338196
47	16.313577081050205	22.29183288886681	41.31474128323021	20.079848746852775
48	19.223306396282002	25.125035314132795	32.09984818803573	23.551810101549474
49	18.044978563157194	22.01506322393972	39.071866976706644	20.86809123619644
50	18.92666768081612	21.857042181373387	36.40941420450841	22.80687593330208
51	18.433666864322326	21.899729594640323	33.47888447699381	26.187719064043534
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39.0
1	712.5
2	1386.0
3	17127.5
4	32869.0
5	25459.0
6	18049.0
7	17571.5
8	17094.0
9	17088.5
10	17083.0
11	16944.5
12	16806.0
13	16317.5
14	15829.0
15	15347.0
16	14865.0
17	14241.5
18	13618.0
19	13002.5
20	12387.0
21	11682.5
22	10978.0
23	10391.0
24	9804.0
25	9565.0
26	9149.0
27	8972.0
28	9005.0
29	9038.0
30	9761.0
31	10484.0
32	11339.5
33	12195.0
34	13284.0
35	14373.0
36	15537.5
37	16702.0
38	17730.5
39	18759.0
40	20488.0
41	22217.0
42	23227.0
43	24237.0
44	26405.0
45	28573.0
46	68625.0
47	108677.0
48	75738.0
49	42799.0
50	38627.5
51	34456.0
52	29971.0
53	25486.0
54	23619.0
55	21752.0
56	19320.0
57	16888.0
58	15278.5
59	13669.0
60	11323.5
61	8978.0
62	7573.0
63	6168.0
64	4990.5
65	3813.0
66	3046.0
67	2279.0
68	1832.0
69	1385.0
70	1128.0
71	871.0
72	709.0
73	547.0
74	452.0
75	280.5
76	204.0
77	153.0
78	102.0
79	82.5
80	63.0
81	46.5
82	30.0
83	17.5
84	5.0
85	4.0
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	644218.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.17475628670979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.77219869305023	42.35847095431964
2	6.6658979760107675	6.822514087088462
3	3.2947853656207124	5.0582951430797385
4	2.0769173792736675	4.251429628478478
5	1.4468264768723809	3.7020496171551525
6	1.0030615497474624	3.0798858209337494
7	0.7137894920535675	2.5569602307098984
8	0.4888483209204313	2.001335494741629
9	0.3487661041653767	1.6063218343555552
>10	1.115861818987033	8.526867099409795
>50	0.04130073749633176	1.4227065115818192
>100	0.027122869423111175	2.8531414477415677
>500	9.246432757878622E-4	0.3175064256898511
>1k	0.002157500976838345	3.1745910729556055
>5k	3.0821442526262076E-4	0.9168727533706431
>10k+	0.001232857701050483	11.351051878388388
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	22931	3.5595093586332576	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	17051	2.6467748495074646	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16829	2.6123144649792462	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	15155	2.352464538401597	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	5813	0.9023343029843934	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	4121	0.6396902911747265	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT	3258	0.5057294269952096	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3066	0.4759258511870206	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA	3045	0.47266608508299984	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	2763	0.42889208311472204	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	2395	0.3717685628156928	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCACAGATCGT	1479	0.22958066989745707	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATG	840	0.13039064416082755	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTAT	672	0.10431251532866204	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.058675789872372396	0.0
2	0.0	0.0	0.0	0.5932774309317653	0.0
3	0.0	0.0	0.0	0.6988317619191019	0.0
4	0.0	0.0	0.0	1.7546855257071363	0.0
5	0.0	0.0	0.0	5.916630705754884	0.0
6	0.0	0.0	0.0	6.433226019763496	0.0
7	0.0	0.0	0.0	7.0201391454445545	0.0
8	0.0	0.0	0.0	7.842997246273777	0.0
9	0.0	0.0	0.0	7.967334039098566	0.0
10	0.0	0.0	0.0	11.126202620851947	0.0
11	0.0	0.0	0.0	11.282826620802275	0.0
12	0.0	0.0	0.0	14.202800915218141	0.0
13	0.0	0.0	0.0	14.45721789828909	0.0
14	0.0	0.0	0.0	14.61834348000211	0.0
15	0.0	0.0	0.0	15.236457224107367	0.0
16	0.0	0.0	0.0	15.5672458701868	0.0
17	0.0	0.0	0.0	15.721851919691781	0.0
18	0.0	0.0	0.0	15.814988094092373	0.0
19	0.0	0.0	0.0	16.537569580483623	0.0
20	1.5522695733431852E-4	0.0	0.0	16.675876799468504	0.0
21	1.5522695733431852E-4	0.0	0.0	16.774756371290465	0.0
22	1.5522695733431852E-4	0.0	0.0	17.01939405604935	0.0
23	1.5522695733431852E-4	0.0	0.0	17.13193360011673	0.0
24	1.5522695733431852E-4	0.0	0.0	17.241989512866763	0.0
25	1.5522695733431852E-4	0.0	0.0	17.328761382016648	0.0
26	1.5522695733431852E-4	0.0	0.0	17.479797211502937	0.0
27	1.5522695733431852E-4	0.0	0.0	17.681747482994886	0.0
28	1.5522695733431852E-4	0.0	0.0	17.776591153926155	0.0
29	1.5522695733431852E-4	0.0	0.0	17.877954357065466	0.0
30	1.5522695733431852E-4	0.0	0.0	17.98769981590083	0.0
31	1.5522695733431852E-4	0.0	0.0	18.099618452138873	0.0
32	1.5522695733431852E-4	0.0	0.0	18.230164323257036	0.0
33	1.5522695733431852E-4	0.0	0.0	18.343790456025754	0.0
34	1.5522695733431852E-4	0.0	0.0	18.51469533605084	0.0
35	1.5522695733431852E-4	0.0	0.0	18.65098460459037	0.0
36	1.5522695733431852E-4	0.0	0.0	18.771285496524467	0.0
37	1.5522695733431852E-4	0.0	0.0	18.912852481613367	0.0
38	1.5522695733431852E-4	0.0	0.0	19.05348810495826	0.0
39	1.5522695733431852E-4	0.0	0.0	19.21771822581797	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	105	0.0	45.000004	17
TTCGTAG	20	7.0309744E-4	45.000004	1
TCGGCGA	20	7.0309744E-4	45.000004	30
GGACGTT	20	7.0309744E-4	45.000004	8
GCGATCG	35	1.2108103E-7	45.000004	9
CGGTGAA	85	0.0	45.0	19
ACCGGTC	65	0.0	44.999996	17
CGTTTTT	17505	0.0	44.408745	1
ACGGCTG	1940	0.0	42.680412	8
CCGGTGA	95	0.0	42.63158	18
CCGATGA	665	0.0	42.293236	18
TGATACC	1900	0.0	42.276318	4
TACGGCT	1970	0.0	42.144672	7
CGGCTGT	1955	0.0	42.122765	9
GATACCT	1905	0.0	42.047245	5
TGTTGCG	65	0.0	41.53846	1
CACCGGT	195	0.0	41.53846	16
CGATGAA	690	0.0	41.413044	19
CGCTTTT	525	0.0	41.142853	1
AATGATC	510	0.0	41.02941	2
>>END_MODULE