##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933335.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357138
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.468395970185195	31.0	27.0	31.0	16.0	33.0
2	28.604491821088768	31.0	28.0	33.0	16.0	34.0
3	28.760025536347296	31.0	30.0	33.0	16.0	34.0
4	32.0720057792786	35.0	30.0	37.0	19.0	37.0
5	33.66970470798403	35.0	33.0	37.0	28.0	37.0
6	32.66827388852489	35.0	33.0	37.0	17.0	37.0
7	32.219366183380096	35.0	32.0	35.0	25.0	37.0
8	32.99359911294793	35.0	32.0	36.0	27.0	37.0
9	33.00216442943624	35.0	31.0	37.0	25.0	39.0
10	31.733817179913647	35.0	28.0	39.0	15.0	39.0
11	32.672762349567954	35.0	30.0	39.0	17.0	39.0
12	33.421248928985435	35.0	31.0	39.0	25.0	39.0
13	33.76500960413062	35.0	32.0	39.0	26.0	39.0
14	33.73488679446041	36.0	32.0	40.0	21.0	41.0
15	34.38231439947583	37.0	32.0	40.0	25.0	41.0
16	34.559456008601714	37.0	32.0	40.0	25.0	41.0
17	32.57100056560769	36.0	29.0	39.0	18.0	41.0
18	33.18867496597954	36.0	30.0	39.0	23.0	39.0
19	33.02034507669304	36.0	31.0	37.0	23.0	39.0
20	32.42467617559599	34.0	30.0	36.0	24.0	39.0
21	32.7375748310177	35.0	31.0	37.0	24.0	39.0
22	33.04862546130627	35.0	31.0	37.0	25.0	39.0
23	33.96982678964434	35.0	33.0	38.0	27.0	40.0
24	32.22010539343335	35.0	31.0	37.0	21.0	39.0
25	31.28394346163108	34.0	28.0	37.0	18.0	39.0
26	30.63035857287659	33.0	25.0	37.0	19.0	39.0
27	32.40213586904782	34.0	30.0	37.0	22.0	39.0
28	32.364721760215936	35.0	31.0	37.0	21.0	39.0
29	32.53151162855815	35.0	31.0	38.0	21.0	40.0
30	31.42851782784246	35.0	29.0	38.0	18.0	40.0
31	31.76691643006345	35.0	30.0	37.0	20.0	40.0
32	31.474878058341595	35.0	30.0	37.0	16.0	40.0
33	31.06909373967486	35.0	29.0	38.0	13.0	40.0
34	29.888443682834087	35.0	24.0	38.0	8.0	40.0
35	29.182192877823137	35.0	22.0	38.0	7.0	40.0
36	28.652798078053863	34.0	20.0	38.0	7.0	40.0
37	28.71453331765312	35.0	20.0	38.0	7.0	40.0
38	28.717814962283487	35.0	20.0	38.0	7.0	40.0
39	28.200213922909352	34.0	18.0	38.0	7.0	40.0
40	28.112975936472736	33.0	18.0	38.0	7.0	40.0
41	27.57936147931612	33.0	18.0	38.0	7.0	40.0
42	28.060979229317518	33.0	18.0	38.0	7.0	40.0
43	28.242654100095763	34.0	18.0	38.0	7.0	40.0
44	28.20502998840784	34.0	18.0	38.0	7.0	40.0
45	28.445746462151885	35.0	18.0	38.0	7.0	40.0
46	28.141099518953457	34.0	18.0	38.0	7.0	40.0
47	28.19059859214085	34.0	18.0	38.0	7.0	40.0
48	27.88434442708421	34.0	18.0	38.0	7.0	40.0
49	27.67144913170819	34.0	17.0	38.0	7.0	40.0
50	27.843287468709576	34.0	17.0	38.0	7.0	40.0
51	26.332431721071405	31.0	15.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	12.0
10	17.0
11	17.0
12	26.0
13	30.0
14	79.0
15	141.0
16	390.0
17	831.0
18	1528.0
19	2424.0
20	3661.0
21	5197.0
22	7189.0
23	9931.0
24	13512.0
25	18407.0
26	21516.0
27	20333.0
28	18316.0
29	17930.0
30	20025.0
31	23061.0
32	26822.0
33	30448.0
34	34314.0
35	35305.0
36	27584.0
37	15021.0
38	3013.0
39	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.366597785729887	2.364072151381259	34.46062866454984	39.80870139833902
2	41.346762315967496	13.12181845673101	32.8427666616266	12.688652565674893
3	10.84482748965386	13.048177455213391	62.880735177998424	13.22625987713433
4	9.843533872060659	2.6345558299592873	72.26954286578298	15.252367432197078
5	18.093846076306637	3.0685617324395613	63.30298092054052	15.534611270713281
6	12.626491720287397	10.903348285536683	65.8672557946788	10.602904199497114
7	51.496620354036814	0.943052825518427	45.193734634791035	2.366592185653725
8	52.71827696856677	7.830866499784397	36.39713500103601	3.0537215306128163
9	49.15998857584463	2.163029417200074	35.77944660047377	12.897535406481527
10	27.136009049723075	14.976563681266065	44.66928750231003	13.218139766700826
11	20.235035196478673	17.350715969737188	48.677542014571394	13.73670681921274
12	14.79596122507266	14.977123688882168	52.84091863649346	17.385996449551712
13	14.819761548757063	16.44658367353796	57.26049874278291	11.473156034922075
14	14.260593944077641	19.681747671768335	50.89125212102885	15.166406263125179
15	11.750079801085295	17.04131176183996	56.9480144929971	14.260593944077641
16	12.540530551215495	16.835508962921896	50.51856705251191	20.10539343335069
17	13.224579854286016	18.182887287267107	52.90559951615342	15.686933342293456
18	13.376901925866191	16.900749850197965	51.929506241284884	17.792841982650963
19	13.820147954012175	19.786189092171654	48.7013423382558	17.692320615560373
20	15.542731381146785	18.595332896527392	53.376565921296525	12.485369801029295
21	15.811535036876501	21.01260577143849	49.90031864433357	13.275540547351444
22	14.177152809278207	15.730053928733431	52.281471028005974	17.81132223398238
23	14.486277013367383	21.056846373110673	50.47236642418337	13.984510189338575
24	15.003164043030987	17.963084297946452	50.00532007235299	17.02843158666958
25	12.902015467410358	22.738829248077774	48.41657846546713	15.94257681904474
26	13.650185642524736	18.138646685594924	51.21129647363204	16.999871198248297
27	15.333288532724046	19.22730149130028	48.732422760949554	16.706987215026125
28	12.371128247344163	19.174380771578495	51.67470277595775	16.77978820511959
29	15.218486971422813	16.522464705519997	49.65895536179292	18.600092961264274
30	13.000296804036534	19.9026706763212	51.31685790926757	15.7801746103747
31	14.397795810023018	18.284808673397958	47.080400293443994	20.236995223135036
32	15.345608700278323	22.540866555785158	45.96906517928644	16.14445956465008
33	13.798027653176085	20.03651249656995	43.60359300886492	22.56186684138904
34	16.533384854034015	23.260196338670205	40.305988161439	19.900430645856783
35	16.39170292715981	22.91355162430209	41.02139789101132	19.67334755752678
36	15.984017382636404	23.38423802563715	36.9112219926191	23.720522599107348
37	15.715213726906684	25.965593132066594	40.85087557190778	17.46831756911894
38	17.719480984941395	23.072313783467454	36.4030150810051	22.805190150586046
39	18.426490600272164	27.265930816659107	36.167811882241594	18.13976670082713
40	19.648427218610173	22.72566906909934	37.87471509612531	19.75118861616518
41	17.253274644535168	27.921699735116395	36.287933515895816	18.537092104452622
42	19.178860832507322	23.506879693563832	39.33241492084292	17.98184455308592
43	18.900537047303843	24.104687823754404	35.96900917852483	21.025765950416925
44	18.744574926218995	23.47187921755736	36.86754139856302	20.916004457660627
45	17.282675044380603	22.616187580151088	36.71913938029557	23.381997995172735
46	20.731761951962547	25.35126477720097	34.537349707956025	19.379623562880454
47	16.4997843970678	22.178821631974195	40.698833504135656	20.62256046682235
48	18.589452816558307	24.30293051985507	34.90919476504881	22.19842189853782
49	17.648640021504292	22.014459396647794	38.61504516461424	21.721855417233673
50	17.99584474348851	22.160621384450828	37.35250799410872	22.49102587795194
51	18.10532623243676	22.034619670827524	35.437281947034485	24.422772149701235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37.0
1	200.0
2	363.0
3	12254.0
4	24145.0
5	17281.5
6	10418.0
7	10111.5
8	9805.0
9	9900.5
10	9996.0
11	9788.5
12	9581.0
13	9286.5
14	8992.0
15	8637.5
16	8283.0
17	7857.5
18	7432.0
19	7061.0
20	6690.0
21	6232.0
22	5774.0
23	5452.0
24	5130.0
25	5000.0
26	4821.5
27	4773.0
28	4759.5
29	4746.0
30	5210.0
31	5674.0
32	6275.5
33	6877.0
34	7450.0
35	8023.0
36	8573.5
37	9124.0
38	9940.0
39	10756.0
40	11934.0
41	13112.0
42	13944.5
43	14777.0
44	15780.5
45	16784.0
46	29074.0
47	41364.0
48	33897.5
49	26431.0
50	24132.0
51	21833.0
52	18820.0
53	15807.0
54	14206.0
55	12605.0
56	11153.5
57	9702.0
58	8718.0
59	7734.0
60	6490.5
61	5247.0
62	4494.5
63	3742.0
64	3113.0
65	2484.0
66	1973.5
67	1463.0
68	1198.5
69	934.0
70	767.5
71	601.0
72	494.0
73	387.0
74	349.5
75	236.0
76	160.0
77	112.5
78	65.0
79	66.5
80	68.0
81	44.5
82	21.0
83	12.5
84	4.0
85	6.0
86	8.0
87	5.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	357138.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.94791402344277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.50065518962246	46.15739563426695
2	7.821294209318326	8.75170191949985
3	3.4541697807165104	5.797607817417045
4	1.9993938633088355	4.474476638536072
5	1.2140920667005288	3.3962959282152565
6	0.8282851408306727	2.78044955076538
7	0.560812956959221	2.196342056943104
8	0.39891749092467593	1.7854881187761025
9	0.2847828153751141	1.433970402296477
>10	0.8644403721505531	7.1791163753324945
>50	0.039391753742439306	1.5341339455395275
>100	0.027113804524016474	2.6575435978235897
>500	0.0025579060871713656	0.9038796641816798
>1k	0.0025579060871713656	2.880794433181953
>5k	0.0010231624348685463	3.463250138251528
>10k+	5.115812174342732E-4	4.607553778972983
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16098	4.507501302017707	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	6504	1.821144767568839	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	5596	1.5669013098578142	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	4728	1.3238580044688608	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	1922	0.5381673190755394	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT	1217	0.3407646343990278	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1108	0.3102442193213828	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	1090	0.3052041507764505	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA	811	0.22708308833000126	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	636	0.17808242192093812	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	585	0.16380222771029687	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCTCACAGATCGTATGCCG	565	0.15820215154926107	No Hit
GAATCTTTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	561	0.1570821363170539	No Hit
GAATCTGTCTCTTATCCACATCTGACGCCTCACAGATCGTATGCCGTCTTC	410	0.11480156130123369	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCACAGATCGT	393	0.11004149656435326	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07784105863839748	0.0
2	0.0	0.0	0.0	0.496166747867771	0.0
3	0.0	0.0	0.0	0.6370086633178211	0.0
4	0.0	0.0	0.0	1.4532197637887876	0.0
5	0.0	0.0	0.0	3.463647105600636	0.0
6	0.0	0.0	0.0	3.9220133393814156	0.0
7	0.0	0.0	0.0	4.328578868672614	0.0
8	0.0	0.0	0.0	4.8849464352715195	0.0
9	0.0	0.0	0.0	5.064428876232717	0.0
10	0.0	0.0	0.0	7.4307410580783895	0.0
11	0.0	0.0	0.0	7.814066271301289	0.0
12	0.0	0.0	0.0	9.767092832462522	0.0
13	0.0	0.0	0.0	10.00173602360992	0.0
14	0.0	0.0	0.0	10.129697763889588	0.0
15	0.0	0.0	0.0	10.52898319417144	0.0
16	0.0	0.0	0.0	10.766146419591307	0.0
17	0.0	0.0	0.0	10.956549009066523	0.0
18	0.0	0.0	0.0	11.150591648046413	0.0
19	0.0	0.0	0.0	11.765480010528144	0.0
20	0.0	0.0	0.0	11.969602786597898	0.0
21	0.0	0.0	0.0	12.199205909200366	0.0
22	0.0	0.0	0.0	12.535490482670564	0.0
23	0.0	0.0	0.0	12.78189383375614	0.0
24	0.0	0.0	0.0	12.997216762147966	0.0
25	0.0	0.0	0.0	13.166339062211247	0.0
26	0.0	0.0	0.0	13.338541404163097	0.0
27	0.0	0.0	0.0	13.524743936517536	0.0
28	0.0	0.0	0.0	13.680986061410435	0.0
29	0.0	0.0	0.0	13.857948468099167	0.0
30	0.0	0.0	0.0	14.055911160391782	0.0
31	0.0	0.0	0.0	14.239033650857651	0.0
32	0.0	0.0	0.0	14.432516282221439	0.0
33	2.800038080517895E-4	0.0	0.0	14.63159898974626	0.0
34	2.800038080517895E-4	0.0	0.0	14.834601750583808	0.0
35	2.800038080517895E-4	0.0	0.0	15.046564633279013	0.0
36	2.800038080517895E-4	0.0	0.0	15.248167375076301	0.0
37	2.800038080517895E-4	0.0	0.0	15.448090094025279	0.0
38	2.800038080517895E-4	0.0	0.0	15.658372953872172	0.0
39	2.800038080517895E-4	0.0	0.0	15.888256080282693	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGGT	55	1.8189894E-12	45.0	4
TAGCGGG	35	1.2087912E-7	45.0	3
CTACGGC	45	3.8380676E-10	45.0	6
GGGCGCA	20	7.0267497E-4	45.0	7
CGAGGGA	20	7.0267497E-4	45.0	4
CGGTGAA	20	7.0267497E-4	45.0	19
ATACCGC	20	7.0267497E-4	45.0	28
CACCGGT	105	0.0	44.999996	16
CGTTTTT	10900	0.0	44.6078	1
GGCGACT	115	0.0	43.043476	10
ACCGGTG	100	0.0	42.750004	17
GATACCT	870	0.0	42.413795	5
CCGGTGT	75	0.0	42.0	18
ACGGCTG	730	0.0	41.917805	8
CGGCTGT	680	0.0	41.691177	9
TACGGCT	735	0.0	41.632652	7
CGGTGTC	65	0.0	41.538464	19
CCGATGA	185	0.0	41.351353	18
GAATGAT	2835	0.0	41.19048	1
CGATGAA	175	0.0	41.142857	19
>>END_MODULE