##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933334.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417731
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.581953458086662	31.0	28.0	33.0	25.0	34.0
2	29.003363408509305	31.0	28.0	33.0	16.0	34.0
3	29.050302706765837	31.0	28.0	34.0	16.0	34.0
4	33.231388142129745	35.0	32.0	37.0	28.0	37.0
5	34.27928738829534	35.0	33.0	37.0	30.0	37.0
6	33.844739796663404	35.0	33.0	37.0	28.0	37.0
7	33.50175352080645	35.0	32.0	37.0	28.0	37.0
8	33.68858906808448	35.0	33.0	37.0	28.0	37.0
9	34.74965468208009	37.0	34.0	39.0	28.0	39.0
10	34.41370403441449	37.0	32.0	39.0	27.0	39.0
11	34.60063294320987	37.0	33.0	39.0	27.0	39.0
12	34.70289731908812	37.0	33.0	39.0	27.0	39.0
13	34.97580021592843	37.0	34.0	39.0	27.0	39.0
14	35.7373908089177	38.0	34.0	40.0	27.0	41.0
15	36.09680871182651	38.0	34.0	40.0	27.0	41.0
16	35.914837538990405	38.0	34.0	40.0	27.0	41.0
17	35.28540615850871	37.0	33.0	40.0	27.0	41.0
18	35.045696871910394	37.0	33.0	39.0	27.0	40.0
19	34.33671190311468	37.0	32.0	38.0	27.0	40.0
20	33.96859462189782	35.0	32.0	38.0	27.0	40.0
21	34.268704022445064	35.0	33.0	38.0	27.0	40.0
22	34.85393470917887	35.0	33.0	39.0	29.0	40.0
23	35.24041308880595	35.0	34.0	39.0	30.0	40.0
24	34.712046747787454	35.0	33.0	39.0	28.0	40.0
25	33.662775805482475	35.0	33.0	38.0	24.0	40.0
26	33.72658002398673	35.0	32.0	38.0	24.0	40.0
27	34.44029291577594	35.0	33.0	39.0	27.0	40.0
28	34.25319164725625	35.0	33.0	39.0	25.0	40.0
29	34.378710222607374	35.0	33.0	39.0	25.0	40.0
30	33.58954446761193	35.0	33.0	39.0	22.0	40.0
31	33.48368926414367	35.0	33.0	39.0	22.0	40.0
32	33.00997053127491	35.0	32.0	39.0	19.0	40.0
33	32.566048485748006	35.0	32.0	39.0	15.0	40.0
34	32.08958157283036	35.0	31.0	40.0	10.0	41.0
35	31.773165027254382	36.0	30.0	40.0	10.0	41.0
36	31.147142539098127	35.0	29.0	40.0	8.0	41.0
37	31.128048911859548	35.0	29.0	40.0	8.0	41.0
38	30.906882658936013	35.0	27.0	39.0	8.0	40.0
39	30.539933593628437	35.0	25.0	39.0	7.0	40.0
40	30.4549889761593	35.0	25.0	39.0	7.0	40.0
41	29.8932016058181	35.0	23.0	39.0	7.0	40.0
42	30.127464803904907	35.0	23.0	39.0	7.0	40.0
43	29.994130193832873	35.0	23.0	39.0	7.0	40.0
44	30.06075201505275	35.0	23.0	39.0	7.0	40.0
45	30.172591931171016	35.0	23.0	39.0	7.0	40.0
46	29.967263621804463	35.0	23.0	39.0	7.0	40.0
47	29.924245507276215	35.0	23.0	39.0	7.0	40.0
48	29.764542253268253	35.0	23.0	38.0	7.0	40.0
49	29.95626132606869	35.0	24.0	39.0	7.0	40.0
50	29.908397988178997	35.0	24.0	39.0	7.0	40.0
51	28.647490849374357	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	13.0
10	23.0
11	27.0
12	35.0
13	43.0
14	94.0
15	104.0
16	200.0
17	366.0
18	720.0
19	1435.0
20	2274.0
21	3354.0
22	4952.0
23	7078.0
24	10303.0
25	15007.0
26	18881.0
27	19758.0
28	18740.0
29	17664.0
30	18358.0
31	20822.0
32	24566.0
33	29335.0
34	33413.0
35	39434.0
36	44241.0
37	46350.0
38	35146.0
39	4989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.794111521529405	2.643806660267014	35.31315607412426	36.24892574407932
2	35.99014676909303	13.720791609911643	35.21117657056814	15.07788505042719
3	13.396180795775273	13.895305830785839	57.45180510902949	15.256708264409394
4	11.932798858595604	3.1965547206216445	67.38140094941481	17.489245471367937
5	20.194814366183024	3.621229930266128	57.99737151420412	18.186584189346732
6	14.74489563858081	12.563587571906323	60.76996919069928	11.921547598813591
7	57.13150328800113	1.2060392932293749	39.09142486432656	2.571032554442931
8	56.594315480536515	8.915306740462164	31.513581706887926	2.9767960721133937
9	53.41260284728689	2.7766672810971653	31.08938527425544	12.7213445973605
10	32.651155887401224	16.86827168680323	37.46956773617471	13.011004689620833
11	25.84270738824746	16.934821691471306	43.175871553703224	14.046599366578013
12	19.59538554715834	16.07278368136432	46.81290112536537	17.51892964611197
13	19.102724001809776	16.126167318202384	51.886979898547146	12.884128781440687
14	16.448384247278753	20.29009099157113	46.76382648163531	16.497698279514807
15	13.908472198615856	15.497772489951666	53.96463274212352	16.629122569308958
16	16.74522599471909	17.620669761162087	45.02083876944732	20.6132654746715
17	17.39612334253383	18.002494428232524	48.41225573395319	16.189126495280455
18	17.746109338306233	16.295893768956578	47.908582317328616	18.049414575408576
19	17.12537494224752	18.771889086517398	44.523150065472755	19.579585905762322
20	19.27125351003397	19.124268967349803	48.39478037301517	13.20969714960106
21	18.88440168433753	21.773342174748823	45.64085499998803	13.70140114092562
22	17.529941517387986	15.234684521857368	48.27341040047303	18.961963560281617
23	17.441367770167883	20.750195700103653	47.414723829450054	14.393712700278408
24	18.537288350637134	17.70397696125018	45.59369546430598	18.16503922380671
25	16.562093787628882	23.928796282775277	43.13230284561117	16.37680708398467
26	16.326296109218614	16.60494433020293	47.33596501097596	19.732794549602495
27	18.724729550835345	17.805717076300297	44.81975242440709	18.64980094845726
28	13.895305830785839	19.58006468277432	48.10583844627284	18.418791040166997
29	19.08836069144976	17.259432505607673	46.12561672463858	17.52659007830398
30	19.36150297679607	19.794556784150565	45.85798037493028	14.985959864123084
31	19.63991180927439	17.364524059741797	45.026105316579326	17.969458814404486
32	22.995420497880215	19.752185018588516	41.707223069391546	15.545171414139721
33	19.557083386198297	18.711323794499332	38.36823218769974	23.363360631602635
34	19.13935044322782	22.845084516112042	42.36027491376029	15.655290126899846
35	20.694178789699592	22.23751648788335	39.23888818402273	17.829416538394323
36	21.789141816144838	22.976030028894193	37.952653741283264	17.2821744136777
37	23.555110824908855	23.29920451199456	36.04376979443709	17.101914868659495
38	21.14829878558211	23.153895688852398	35.623164189394615	20.074641336170885
39	23.817480627485153	24.677364141038133	33.530190481434225	17.974964750042492
40	21.74341861149879	22.003633917521086	37.122215013968315	19.13073245701181
41	22.199214326923308	25.758203245629364	32.87594169453548	19.166640732911848
42	19.97457694066277	22.646631444637816	38.84054571003828	18.53824590466113
43	23.774869473417105	20.90914966808784	34.08724753489686	21.2287333235982
44	23.047367803682274	22.15061846020525	33.65850272065037	21.143511015462106
45	19.87834276125066	21.32353117197431	34.99237547608389	23.805750590691137
46	23.889536567791232	23.243666378602498	32.75217783693334	20.11461921667293
47	17.670941347422144	22.48217154101563	40.072199573409684	19.774687538152545
48	20.15746975924698	25.21143989792474	33.17828937761382	21.452800965214454
49	19.66648393344042	21.924635710540993	38.24111689101359	20.16776346500499
50	20.483516904419353	21.519829746894533	35.77182445162078	22.224828897065336
51	19.58581000691833	21.676868606830713	34.434600257103256	24.302721129147706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55.0
1	691.0
2	1327.0
3	13255.5
4	25184.0
5	18380.0
6	11576.0
7	11008.0
8	10440.0
9	10045.0
10	9650.0
11	9230.0
12	8810.0
13	8221.0
14	7632.0
15	7273.5
16	6915.0
17	6310.0
18	5705.0
19	5397.5
20	5090.0
21	4810.0
22	4530.0
23	4484.0
24	4438.0
25	4556.5
26	4919.5
27	5164.0
28	5382.0
29	5600.0
30	6171.0
31	6742.0
32	7278.0
33	7814.0
34	8693.0
35	9572.0
36	10041.0
37	10510.0
38	11061.0
39	11612.0
40	12129.5
41	12647.0
42	13332.0
43	14017.0
44	14964.0
45	15911.0
46	33461.5
47	51012.0
48	37335.5
49	23659.0
50	22395.5
51	21132.0
52	19678.5
53	18225.0
54	18286.0
55	18347.0
56	17889.0
57	17431.0
58	16433.0
59	15435.0
60	13932.0
61	12429.0
62	11169.0
63	9909.0
64	8722.0
65	7535.0
66	6567.0
67	5599.0
68	4769.5
69	3940.0
70	3326.0
71	2712.0
72	2284.0
73	1856.0
74	1592.5
75	1090.0
76	851.0
77	642.0
78	433.0
79	285.5
80	138.0
81	103.0
82	68.0
83	49.0
84	30.0
85	25.5
86	21.0
87	18.5
88	16.0
89	9.5
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	417731.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.37969387448662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91377440584321	39.75651797046678
2	7.694852685043825	7.753286453635587
3	4.25343828338703	6.428607558931813
4	2.826305404575479	5.695536043132789
5	1.9517133337922936	4.916336014360474
6	1.3199195544089353	3.989828585404257
7	0.9277795265348403	3.2718873970888978
8	0.5685547415902056	2.291489106576192
9	0.40497009779801396	1.8362042599846367
>10	1.0553564267678346	8.097577789048785
>50	0.050097665820063676	1.798767727195783
>100	0.027457374535983285	2.3930988340355346
>500	9.634166503853785E-4	0.3494637786239904
>1k	0.0028902499511561353	2.4768245309975323
>5k	0.0014451249755780677	5.982383060319436
>10k+	4.8170832519268925E-4	2.962190890197519
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12206	2.9219761042393313	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	9026	2.1607206551584635	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	8893	2.128881983860427	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	6732	1.611563422393837	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	2211	0.5292879867666034	TruSeq Adapter, Index 3 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGCATGT	1959	0.46896208325453465	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTC	1726	0.4131845613564711	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1625	0.38900632225044346	RNA PCR Primer, Index 24 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTA	1423	0.3406498440383883	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	1262	0.3021082945723444	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTT	772	0.18480792663221068	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGT	668	0.1599115220081823	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04668075867005322	0.0
2	0.0	0.0	0.0	0.4919433798305608	0.0
3	0.0	0.0	0.0	0.5778838534846588	0.0
4	0.0	0.0	0.0	1.2022090771333704	0.0
5	0.0	0.0	0.0	3.721054937268242	0.0
6	0.0	0.0	0.0	4.003054597336564	0.0
7	0.0	0.0	0.0	4.42102692881304	0.0
8	0.0	0.0	0.0	4.985265637455683	0.0
9	0.0	0.0	0.0	5.096820681251811	0.0
10	0.0	0.0	0.0	7.652292982804724	0.0
11	0.0	0.0	0.0	7.818907382980914	0.0
12	0.0	0.0	0.0	9.857779288585238	0.0
13	0.0	0.0	0.0	10.021999803701425	0.0
14	0.0	0.0	0.0	10.168505569373592	0.0
15	0.0	0.0	0.0	10.619513514678106	0.0
16	0.0	0.0	0.0	10.86775939540039	0.0
17	0.0	0.0	0.0	10.987932425412525	0.0
18	0.0	0.0	0.0	11.071718402512621	0.0
19	0.0	0.0	0.0	11.733867010109376	0.0
20	0.0	0.0	0.0	11.863854968867525	0.0
21	0.0	0.0	0.0	11.94788033447362	0.0
22	0.0	0.0	0.0	12.162611824355865	0.0
23	0.0	0.0	0.0	12.27177298309199	0.0
24	0.0	0.0	0.0	12.365373888938096	0.0
25	0.0	0.0	0.0	12.442217599364184	0.0
26	0.0	0.0	0.0	12.539888109812296	0.0
27	0.0	2.393885060002729E-4	0.0	12.679212220304453	0.0
28	0.0	2.393885060002729E-4	0.0	12.769222298560557	0.0
29	0.0	2.393885060002729E-4	0.0	12.860429319346661	0.0
30	0.0	2.393885060002729E-4	0.0	12.986587062008805	0.0
31	0.0	2.393885060002729E-4	0.0	13.081145521878913	0.0
32	0.0	2.393885060002729E-4	0.0	13.19317934268704	0.0
33	0.0	2.393885060002729E-4	0.0	13.300186004869163	0.0
34	0.0	2.393885060002729E-4	0.0	13.458661195841342	0.0
35	0.0	2.393885060002729E-4	0.0	13.592958147707495	0.0
36	0.0	2.393885060002729E-4	0.0	13.721988552441642	0.0
37	0.0	2.393885060002729E-4	0.0	13.871845757197814	0.0
38	0.0	2.393885060002729E-4	0.0	14.048275086120015	0.0
39	0.0	2.393885060002729E-4	0.0	14.32524758756233	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	20	7.028124E-4	45.000004	2
TACGGGT	20	7.028124E-4	45.000004	4
CCCACGA	25	3.8865277E-5	45.0	39
CTACGGG	25	3.8865277E-5	45.0	3
TGATGCG	25	3.8865277E-5	45.0	4
CGTTTTT	9590	0.0	43.92075	1
GATACCT	1060	0.0	43.726414	5
ATACCTG	1080	0.0	42.916668	6
TGATACC	1075	0.0	42.906975	4
TACGGCT	830	0.0	42.560238	7
ACGGCTG	855	0.0	41.57895	8
TACCTGT	1140	0.0	41.44737	7
CTACGAA	60	3.6379788E-12	41.249996	11
TACGAAT	60	3.6379788E-12	41.249996	12
ACCCGTC	60	3.6379788E-12	41.249996	17
GCTACGA	60	3.6379788E-12	41.249996	10
CACCCGT	60	3.6379788E-12	41.249996	16
TACGGTG	55	6.002665E-11	40.909092	7
AATGATC	260	0.0	40.673077	2
TCGTTGA	50	1.0786607E-9	40.5	24
>>END_MODULE