##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933333.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	317597
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.85179016174586	31.0	28.0	31.0	16.0	33.0
2	29.416150026606044	31.0	30.0	33.0	16.0	34.0
3	29.633227014109075	31.0	30.0	33.0	16.0	34.0
4	32.25784563456204	35.0	32.0	37.0	19.0	37.0
5	33.59300308252282	35.0	33.0	37.0	28.0	37.0
6	33.096798773288164	35.0	33.0	37.0	26.0	37.0
7	31.6861053473427	35.0	30.0	35.0	23.0	37.0
8	33.131906157803755	35.0	33.0	35.0	28.0	37.0
9	32.69644864403631	35.0	31.0	37.0	25.0	39.0
10	30.724896645749173	35.0	27.0	39.0	15.0	39.0
11	31.510140209132956	35.0	27.0	39.0	15.0	39.0
12	32.53053712723987	35.0	30.0	39.0	19.0	39.0
13	32.752588972817755	35.0	31.0	39.0	21.0	39.0
14	33.037777434925395	36.0	31.0	39.0	17.0	41.0
15	33.73878216733785	36.0	31.0	39.0	23.0	41.0
16	33.91864217861	36.0	32.0	40.0	23.0	41.0
17	31.92934127211529	35.0	27.0	39.0	17.0	41.0
18	32.7368961293715	36.0	30.0	39.0	21.0	39.0
19	32.4931060431931	36.0	30.0	37.0	18.0	39.0
20	32.429475089500215	34.0	30.0	37.0	24.0	39.0
21	32.65901755998954	35.0	31.0	37.0	23.0	39.0
22	32.9961114242263	35.0	31.0	38.0	25.0	39.0
23	34.12712336703432	35.0	33.0	38.0	27.0	40.0
24	32.260137847649695	35.0	30.0	37.0	21.0	39.0
25	31.37023964332157	34.0	29.0	37.0	18.0	39.0
26	30.783502363057586	33.0	25.0	37.0	19.0	39.0
27	32.34624382472126	34.0	30.0	38.0	22.0	39.0
28	32.42429871818688	35.0	31.0	38.0	21.0	39.0
29	32.818439720778215	35.0	31.0	38.0	22.0	40.0
30	31.66873112781292	35.0	30.0	38.0	18.0	40.0
31	31.946246343636748	35.0	30.0	38.0	21.0	40.0
32	31.805590103181075	35.0	30.0	38.0	18.0	40.0
33	31.912971470133535	35.0	30.0	38.0	16.0	40.0
34	31.19745463590651	35.0	30.0	38.0	12.0	40.0
35	30.671105205653706	35.0	29.0	38.0	10.0	40.0
36	30.077459799683247	35.0	26.0	38.0	8.0	40.0
37	30.259848172369388	35.0	27.0	38.0	8.0	40.0
38	30.22658904208793	35.0	26.0	38.0	8.0	40.0
39	29.787601898002816	34.0	25.0	38.0	8.0	40.0
40	29.67634454985406	34.0	24.0	38.0	8.0	40.0
41	29.13504850486623	34.0	23.0	38.0	8.0	40.0
42	29.578859372097345	34.0	24.0	38.0	8.0	40.0
43	29.553317569120615	34.0	23.0	38.0	7.0	40.0
44	29.527719090545567	34.0	23.0	38.0	7.0	40.0
45	29.74464179447539	35.0	24.0	38.0	7.0	40.0
46	29.5769985232858	34.0	23.0	38.0	7.0	40.0
47	29.675963563887567	34.0	24.0	38.0	7.0	40.0
48	29.335623447324753	34.0	23.0	38.0	7.0	40.0
49	29.082847130168105	34.0	24.0	37.0	7.0	39.0
50	29.295364880650634	34.0	24.0	37.0	7.0	40.0
51	27.733715998576812	32.0	22.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	20.0
10	18.0
11	34.0
12	33.0
13	58.0
14	81.0
15	202.0
16	336.0
17	615.0
18	1065.0
19	1588.0
20	2577.0
21	3562.0
22	5056.0
23	6913.0
24	9271.0
25	12615.0
26	15700.0
27	16822.0
28	17023.0
29	17958.0
30	19751.0
31	22645.0
32	25576.0
33	29352.0
34	32659.0
35	34737.0
36	25545.0
37	13084.0
38	2642.0
39	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.082683400661843	2.7934772683621065	39.11560877464208	38.00823055633397
2	38.404330015711736	7.675765199293444	38.9698265411827	14.950078243812126
3	14.49950723715904	7.734644848660409	62.3806270210361	15.385220893144458
4	13.127642893352268	3.2859252448858145	65.41969854878982	18.1667333129721
5	16.769364949920814	3.7012314348057442	61.4772809566841	18.052122658589344
6	15.542023381832951	8.003853940685838	63.45085123600034	13.003271441480871
7	59.20144081965509	1.3476197823027296	36.592914920480986	2.858024477561186
8	61.150136808597054	5.238084742614067	30.731713460769466	2.880064988019408
9	57.083032900184826	2.7714367579038846	31.376555823890023	8.768974518021267
10	31.891674039742192	17.98946463600097	37.40558002751915	12.713281296737689
11	24.29210603374717	18.13587659833059	43.82220235077787	13.749815017144368
12	19.683435296932906	16.730321759966245	47.28004357723782	16.306199365863026
13	18.269064254385274	16.929945811830716	50.91641293840937	13.884576995374642
14	16.428051902253486	17.44758294316382	48.85908871935188	17.26527643523081
15	14.598374669785924	16.025025425303134	52.47908512989732	16.897514775013615
16	17.082969927297803	15.96740523367664	47.22242338561132	19.727201453414235
17	17.61603541595166	16.84934051644065	48.68150517794563	16.853118889662056
18	18.003948400016373	15.59555033580292	48.41355554366068	17.98694572052003
19	17.72403391719695	18.043306454406054	45.74759837152114	18.485061256875852
20	19.45326939486204	18.51528824264713	47.86285764664024	14.16858471585059
21	19.60094081493213	19.294262855127727	45.997285868569286	15.107510461370858
22	18.415161352279778	16.217407595159905	47.70101732698986	17.666413725570457
23	17.951366039351758	19.594328661794663	46.56498644508607	15.889318853767511
24	17.412318126430666	17.58549356574527	47.03917228437297	17.963016023451104
25	17.33706552643759	19.683120432497788	44.879202259467185	18.100611781597433
26	16.623897580896546	17.743240647739118	46.994461534586286	18.638400236778054
27	16.9567092888157	17.793933821793026	46.546094578979016	18.703262310412253
28	15.07633888229423	19.114160398240536	48.037922272565545	17.77157844689969
29	18.34903982090511	17.37957222517845	46.220524753067565	18.050863200848873
30	18.2803993740495	18.584243553937853	46.500754100322105	16.634602971690537
31	18.79016489450467	18.406345148096488	45.50798653639676	17.29550342100209
32	20.394084326993013	19.477513956366085	43.60431616167659	16.52408555496431
33	19.19224677814967	18.73569334722935	41.44780964555711	20.624250229063875
34	18.51875175143342	21.87709581639625	42.74851462702733	16.855637805142994
35	19.256479122913632	22.680944719251126	40.21983834859901	17.842737809236233
36	20.745787901019217	22.47502337868431	39.0601926340614	17.718996086235073
37	22.092148225581475	23.14190625226309	37.41534082500779	17.35060469714764
38	20.513417947902532	22.870807973626956	38.05703454377717	18.558739534693338
39	21.602534028973825	23.94858893503402	36.137620947301144	18.311256088691014
40	21.470920695094726	22.496434160272294	37.708164749667034	18.324480394965946
41	20.744213578843627	24.05532797853884	36.53120149119796	18.669256951419566
42	19.83645941239999	22.597190779509884	38.73651199476066	18.82983781332947
43	21.399761332758178	21.313803341971113	37.202807331303504	20.083627993967198
44	21.612924555332704	22.366080284133666	36.04945890546825	19.97153625506538
45	19.369515455120798	21.833329659914924	37.17604385431852	21.621111030645757
46	21.54963680387409	22.794925644763648	35.6212432737085	20.034194277653754
47	18.117929325528895	22.41519913601199	40.11530335614001	19.351568182319102
48	19.679656923711498	23.809103990276988	36.28088426527958	20.230354820731932
49	19.288910159730726	22.25146962975091	38.57876491276681	19.880855297751555
50	19.80938107097989	21.959590298397025	37.21508704427309	21.01594158635
51	19.282927735463495	21.491386883377363	37.02648324763773	22.19920213352141
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	370.0
2	672.0
3	14045.0
4	27418.0
5	18161.0
6	8904.0
7	8339.0
8	7774.0
9	7550.0
10	7326.0
11	6976.5
12	6627.0
13	6225.0
14	5823.0
15	5423.0
16	5023.0
17	4585.0
18	4147.0
19	3792.5
20	3438.0
21	3229.0
22	3020.0
23	2978.5
24	2937.0
25	2970.5
26	3238.0
27	3472.0
28	3715.0
29	3958.0
30	4396.0
31	4834.0
32	5325.0
33	5816.0
34	6610.0
35	7404.0
36	7684.0
37	7964.0
38	8671.5
39	9379.0
40	9743.5
41	10108.0
42	10700.0
43	11292.0
44	11873.5
45	12455.0
46	18381.0
47	24307.0
48	21588.5
49	18870.0
50	17955.5
51	17041.0
52	16177.0
53	15313.0
54	14564.0
55	13815.0
56	13488.0
57	13161.0
58	12379.0
59	11597.0
60	10629.0
61	9661.0
62	8971.5
63	8282.0
64	7358.0
65	6434.0
66	5802.0
67	5170.0
68	4500.0
69	3830.0
70	3211.0
71	2592.0
72	2206.0
73	1820.0
74	1551.0
75	1048.5
76	815.0
77	629.0
78	443.0
79	303.5
80	164.0
81	119.5
82	75.0
83	52.0
84	29.0
85	24.0
86	19.0
87	12.5
88	6.0
89	5.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	317597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.01213320079605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.33755879027004	39.451538504336504
2	8.712585839885584	8.888951787752259
3	4.491888825134479	6.87422493212782
4	2.8444270561987346	5.804011674830321
5	1.9960360614041284	5.091102871896987
6	1.3227090935183399	4.048452747867705
7	0.9294122630974354	3.318791151450578
8	0.6727880273648975	2.745628197427118
9	0.4617665687503398	2.1200127941490194
>10	1.1533987954769171	8.517610413494207
>50	0.05098961781161301	1.7754842039808045
>100	0.022032550906252533	2.0397677091130535
>500	0.001259002908928716	0.4386528165378512
>1k	0.002518005817857432	2.8438574987256295
>5k	0.0	0.0
>10k+	6.29501454464358E-4	6.0419126963101535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18815	5.924174346735013	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	3475	1.094153912033174	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	2308	0.7267071162510981	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	2057	0.6476761430366156	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGCATGT	1016	0.3199022660793395	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	697	0.21946051127686972	TruSeq Adapter, Index 3 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	669	0.21064430709358084	RNA PCR Primer, Index 24 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTC	469	0.14767142007008882	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTA	433	0.13633630040586026	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07619719329842536	0.0
2	0.0	0.0	0.0	0.39578459494264745	0.0
3	0.0	0.0	0.0	0.5129141648063427	0.0
4	0.0	0.0	0.0	0.8857136559854155	0.0
5	0.0	0.0	0.0	1.7600921923066024	0.0
6	0.0	0.0	0.0	2.053545845836075	0.0
7	0.0	0.0	0.0	2.3000846985330465	0.0
8	0.0	0.0	0.0	2.6653274432693004	0.0
9	0.0	0.0	0.0	2.849523137813015	0.0
10	0.0	0.0	0.0	4.337572458178132	0.0
11	0.0	0.0	0.0	4.756027292449236	0.0
12	0.0	0.0	0.0	5.900244649666086	0.0
13	0.0	0.0	0.0	6.048860663041528	0.0
14	0.0	0.0	0.0	6.155599706546346	0.0
15	0.0	0.0	0.0	6.421030425350366	0.0
16	0.0	0.0	0.0	6.597354509016143	0.0
17	0.0	0.0	0.0	6.736209724902943	0.0
18	0.0	0.0	0.0	6.877583856270682	0.0
19	0.0	0.0	0.0	7.3876642411609685	0.0
20	0.0	0.0	0.0	7.551708611857165	0.0
21	0.0	0.0	0.0	7.704102998454015	0.0
22	0.0	0.0	0.0	7.939621595921876	0.0
23	0.0	0.0	0.0	8.124761883770942	0.0
24	0.0	0.0	0.0	8.2736927615815	0.0
25	0.0	0.0	0.0	8.411603384162948	0.0
26	0.0	0.0	0.0	8.548884277874162	0.0
27	0.0	0.0	0.0	8.69403678246331	0.0
28	0.0	0.0	0.0	8.822501471991234	0.0
29	0.0	0.0	0.0	8.969543163191089	0.0
30	0.0	0.0	0.0	9.139569958154517	0.0
31	0.0	0.0	0.0	9.264886003331267	0.0
32	0.0	0.0	0.0	9.423892543065584	0.0
33	0.0	0.0	0.0	9.581324760624312	0.0
34	0.0	0.0	0.0	9.743794809144923	0.0
35	0.0	0.0	0.0	9.929249961429107	0.0
36	0.0	0.0	0.0	10.09360919656042	0.0
37	0.0	0.0	0.0	10.257968431691735	0.0
38	0.0	0.0	0.0	10.452554652594326	0.0
39	0.0	0.0	0.0	10.759547476833848	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGCT	20	7.025567E-4	45.000004	34
CGATGAA	75	0.0	45.000004	19
AGATGCG	20	7.025567E-4	45.000004	10
CGTTTTT	8530	0.0	44.261433	1
CGACCAC	370	0.0	42.567566	12
GCGATTC	80	0.0	42.187504	9
TACGGCT	270	0.0	40.833332	7
GAATGAT	1385	0.0	40.61372	1
TGGGACG	50	1.0768417E-9	40.5	6
TACGGCG	595	0.0	40.462185	7
GCGACCA	395	0.0	40.443035	11
ATACGGC	865	0.0	40.317917	6
GAATCTG	330	0.0	40.227272	1
CCGATGA	90	0.0	40.0	18
ACGGCGA	585	0.0	40.0	8
TGATACC	445	0.0	39.943817	4
ACCACCG	175	0.0	39.857143	14
AATGATA	1325	0.0	39.73585	2
CGGCGAC	590	0.0	39.66102	9
AATCTGT	335	0.0	39.626865	2
>>END_MODULE