##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933332.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	428249
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.843735770544708	31.0	28.0	33.0	25.0	34.0
2	29.43397883007316	31.0	30.0	33.0	16.0	34.0
3	29.386637213396877	31.0	28.0	34.0	16.0	34.0
4	33.58837732253899	35.0	32.0	37.0	28.0	37.0
5	34.45354922019666	35.0	35.0	37.0	32.0	37.0
6	34.13486546378392	35.0	35.0	37.0	29.0	37.0
7	33.87886486600085	35.0	33.0	37.0	29.0	37.0
8	34.01028373679799	35.0	35.0	37.0	29.0	37.0
9	35.060341063259926	37.0	34.0	39.0	29.0	39.0
10	34.93153982846428	37.0	34.0	39.0	28.0	39.0
11	35.054804564634125	37.0	34.0	39.0	27.0	39.0
12	35.152014365474294	37.0	34.0	39.0	27.0	39.0
13	35.30149048801048	37.0	34.0	39.0	29.0	39.0
14	36.134512865178905	38.0	34.0	40.0	27.0	41.0
15	36.50352715359522	38.0	34.0	41.0	30.0	41.0
16	36.429710285371364	38.0	34.0	41.0	30.0	41.0
17	35.76006248701106	38.0	33.0	40.0	27.0	41.0
18	35.52468540498635	38.0	34.0	39.0	28.0	40.0
19	34.721356033522554	37.0	33.0	38.0	27.0	40.0
20	34.173959542228936	35.0	33.0	38.0	27.0	40.0
21	34.339143815864134	35.0	33.0	38.0	27.0	40.0
22	34.85631023073025	35.0	33.0	39.0	30.0	40.0
23	35.26609752737309	35.0	34.0	39.0	30.0	40.0
24	34.70191640844462	35.0	33.0	39.0	29.0	40.0
25	33.63213457591261	35.0	33.0	38.0	24.0	40.0
26	33.67157424769235	35.0	33.0	38.0	24.0	40.0
27	34.45705419043594	35.0	33.0	38.0	27.0	40.0
28	34.29114370377981	35.0	33.0	39.0	25.0	40.0
29	34.25561764300676	35.0	33.0	39.0	25.0	40.0
30	33.515947497834205	35.0	33.0	39.0	22.0	40.0
31	33.34618644760408	35.0	33.0	39.0	21.0	40.0
32	32.655037139608034	35.0	32.0	39.0	18.0	40.0
33	32.01623121128129	35.0	31.0	39.0	14.0	40.0
34	31.24397254868079	35.0	29.0	39.0	10.0	40.0
35	30.748176878404852	35.0	25.0	40.0	8.0	41.0
36	30.01127615009025	35.0	23.0	39.0	7.0	41.0
37	29.86837797636422	35.0	22.0	39.0	7.0	41.0
38	29.637909253728555	35.0	21.0	39.0	7.0	40.0
39	29.258352033513212	35.0	20.0	39.0	7.0	40.0
40	29.106199897723055	35.0	20.0	39.0	7.0	40.0
41	28.70081424591768	35.0	18.0	39.0	7.0	40.0
42	28.796711726121952	35.0	18.0	39.0	7.0	40.0
43	28.644596951773384	35.0	18.0	39.0	7.0	40.0
44	28.68297649264797	35.0	18.0	39.0	7.0	40.0
45	28.746509624073845	35.0	18.0	39.0	7.0	40.0
46	28.597126905141636	35.0	18.0	39.0	7.0	40.0
47	28.464605871817565	35.0	18.0	39.0	7.0	40.0
48	28.370814642882994	34.0	18.0	38.0	7.0	40.0
49	28.52055229551032	35.0	18.0	39.0	7.0	40.0
50	28.493215395716042	35.0	18.0	39.0	7.0	40.0
51	27.47943369394908	33.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	11.0
10	20.0
11	24.0
12	27.0
13	30.0
14	73.0
15	104.0
16	199.0
17	407.0
18	857.0
19	1528.0
20	2387.0
21	3681.0
22	5524.0
23	8002.0
24	11933.0
25	17385.0
26	23238.0
27	24439.0
28	22370.0
29	19928.0
30	19323.0
31	20758.0
32	23228.0
33	26729.0
34	30148.0
35	35838.0
36	41014.0
37	45321.0
38	37537.0
39	6181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.421654224528254	2.402574203325635	32.770420946692234	41.405350625453885
2	41.428000999418565	12.294015864602136	31.694177919855036	14.583805216124265
3	11.966402723649091	12.467746568001328	61.29237896644242	14.273471741907162
4	10.25197957263181	2.6599011322851895	70.7539305404099	16.3341887546731
5	18.202027325224343	3.1220154629666386	62.50008756587873	16.175869645930288
6	13.350644134603934	10.710124250144192	64.64066466004591	11.298566955205967
7	52.201406191257895	0.9917127652370467	44.53670644881832	2.270174594686736
8	52.36836513336867	7.400134034171709	37.73949267832499	2.4920081541346275
9	49.44483232885541	2.5109223839401844	37.07936270721006	10.964882579994349
10	28.392360519230635	19.748557498091063	41.986554551207355	9.872527431470942
11	22.14109081398906	15.231559209712106	50.3597206298205	12.267629346478333
12	17.639737629276425	15.28970295318845	51.99498422646638	15.075575191068747
13	16.831562945856266	15.465768746687091	56.405268897300395	11.29739941015624
14	14.696823576937716	19.233903640171956	51.74699765790463	14.322275124985698
15	12.316199220546926	15.149130529201468	58.0778939355375	14.456776314714103
16	15.186958988812583	17.373770866948902	50.05615891689181	17.383111227346706
17	15.47394156203517	17.239736695240385	53.5053205027916	13.781001239932845
18	16.436932719048965	15.40902605727042	52.52248107993247	15.631560143748146
19	15.028873389079717	17.813468332675615	50.197665376918565	16.9599929013261
20	17.12741886145677	18.62911530441402	53.17046858253026	11.072997251598952
21	16.334655772692987	21.307930666504767	51.06538485787474	11.292028702927501
22	15.370730579639416	14.657594063266933	53.72832160728922	16.243353749804438
23	14.501610044623572	19.93162856188806	53.206428970061815	12.360332423426557
24	16.102547816807512	16.872427022596668	51.44460349002566	15.580421670570157
25	14.504178643732967	23.19561750290135	48.45195201856864	13.848251834797045
26	14.926129424703852	16.409845673895326	51.41891749893169	17.245107402469124
27	16.83670014407506	17.11761148303907	49.76777528960955	16.277913083276317
28	11.915264250471104	19.788954556811575	51.9889129922078	16.306868200509516
29	16.233779880396686	19.92065363842064	48.62731728503744	15.218249196145234
30	17.51434329093588	19.448264911301603	50.59719929293471	12.440192504827799
31	17.78824935960154	19.612888763312935	47.222527081207424	15.3763347958781
32	18.641024263921224	22.84115082580461	45.76379629608008	12.75402861419408
33	19.50851023586745	19.08796050895624	42.72467653164397	18.678852723532337
34	17.486322209742465	23.767948086276906	43.257544092338804	15.488185611641825
35	17.987432545084754	25.523702332054484	40.44072490537047	16.048140217490293
36	19.691814808674394	28.09206793244117	36.94112537332253	15.274991885561903
37	19.28130596919082	26.427615709552153	38.415968280136084	15.875110041120937
38	21.279209058281516	26.618626079687285	36.06243096889894	16.039733893132265
39	20.271851189378143	24.922883649465614	35.6091899805954	19.196075180560843
40	23.156621498240508	24.601808760791037	36.87457530548816	15.366994435480294
41	20.503725636253677	25.177408470305828	34.78443615746913	19.53442973597136
42	18.7577787688938	25.81325350438647	38.60370952413199	16.825258202587747
43	22.629358153784363	22.742376514597815	35.66406459793251	18.96420073368531
44	21.990243993564494	23.16292624150903	35.05904275316463	19.78778701176185
45	18.866593967528235	23.128133399027202	35.43639331323599	22.56887932020857
46	21.55661776209635	24.52124815235996	34.53504853484772	19.387085550695975
47	17.21171561404697	23.640685675856805	41.455321553582145	17.692277156514084
48	18.433434754079986	25.58581572869989	34.97217740146504	21.008572115755086
49	18.27394810028745	23.19024679567261	39.233016305934164	19.302788798105773
50	19.05947240974293	22.397950724928723	37.86278543557603	20.679791429752317
51	17.664256075320665	22.230524764798048	35.950580153135206	24.154639006746077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	703.5
2	1361.0
3	18008.5
4	34656.0
5	25207.0
6	15758.0
7	14653.5
8	13549.0
9	13012.5
10	12476.0
11	12006.5
12	11537.0
13	10727.5
14	9918.0
15	9217.0
16	8516.0
17	7822.5
18	7129.0
19	6680.5
20	6232.0
21	5695.5
22	5159.0
23	5040.5
24	4922.0
25	4948.0
26	5300.0
27	5626.0
28	6066.5
29	6507.0
30	7093.5
31	7680.0
32	8209.5
33	8739.0
34	9358.0
35	9977.0
36	10423.0
37	10869.0
38	11626.0
39	12383.0
40	12826.5
41	13270.0
42	13854.0
43	14438.0
44	15407.5
45	16377.0
46	32873.0
47	49369.0
48	35934.0
49	22499.0
50	21773.0
51	21047.0
52	19908.0
53	18769.0
54	18034.5
55	17300.0
56	16286.0
57	15272.0
58	14275.0
59	13278.0
60	11544.0
61	9810.0
62	8412.5
63	7015.0
64	5784.0
65	4553.0
66	3611.5
67	2670.0
68	2136.0
69	1602.0
70	1317.0
71	1032.0
72	854.0
73	676.0
74	617.0
75	480.0
76	402.0
77	290.0
78	178.0
79	125.5
80	73.0
81	51.0
82	29.0
83	18.5
84	8.0
85	6.5
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	428249.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.58375334851176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74031249939799	43.525255494003886
2	9.260078039527118	10.108996313950373
3	4.544832581095109	7.442220618503266
4	2.5808324369519875	5.634860846897032
5	1.4540674060239398	3.9684228321260515
6	0.8224485072390819	2.693539587659384
7	0.4561002517028672	1.7426964548800403
8	0.27654058015628213	1.207569825448388
9	0.17510450668874147	0.8602075082979983
>10	0.5942303570308834	5.549808324586767
>50	0.05571681271530494	2.128627179345815
>100	0.034553062148463244	3.2866531738727645
>500	4.319132768557906E-4	0.12872194483426794
>1k	0.003023392937990534	2.337741401056046
>5k	0.0012957398305673716	5.606005725666772
>10k+	4.319132768557906E-4	3.7786727688711474
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16028	3.742682411400844	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	9020	2.1062512697052416	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	7780	1.8167000973732572	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC	6979	1.6296593804071928	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCT	1901	0.44390062790572776	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1784	0.41658007374214534	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTC	1440	0.3362529743210142	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAAGCAGT	1304	0.3044957489684739	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCT	1291	0.301460131839187	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTT	1123	0.26223061816840204	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTA	1073	0.2505551676711446	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGT	546	0.1274959194300512	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	498	0.11628748695268407	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	491	0.11465292388306804	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	441	0.1029774733858106	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3350900994514873E-4	0.0	0.0	0.04086407674040103	0.0
2	2.3350900994514873E-4	0.0	0.0	0.5319335246550488	0.0
3	2.3350900994514873E-4	0.0	0.0	0.6166972952651378	0.0
4	2.3350900994514873E-4	0.0	0.0	1.1628748695268407	0.0
5	2.3350900994514873E-4	0.0	0.0	3.6348012488061854	0.0
6	2.3350900994514873E-4	0.0	0.0	4.029431475613486	0.0
7	2.3350900994514873E-4	0.0	0.0	4.473098594509269	0.0
8	2.3350900994514873E-4	0.0	0.0	4.980046655100187	0.0
9	2.3350900994514873E-4	0.0	0.0	5.121086097107057	0.0
10	2.3350900994514873E-4	0.0	0.0	7.365574700699827	0.0
11	2.3350900994514873E-4	0.0	0.0	7.564524377173093	0.0
12	2.3350900994514873E-4	0.0	0.0	9.575970988840604	0.0
13	2.3350900994514873E-4	0.0	0.0	9.737325714712702	0.0
14	2.3350900994514873E-4	0.0	0.0	9.858049872854345	0.0
15	2.3350900994514873E-4	0.0	0.0	10.22162340133894	0.0
16	2.3350900994514873E-4	0.0	0.0	10.43154800127963	0.0
17	2.3350900994514873E-4	0.0	0.0	10.545033380112972	0.0
18	2.3350900994514873E-4	0.0	0.0	10.630497677752896	0.0
19	2.3350900994514873E-4	0.0	0.0	11.096348152593468	0.0
20	2.3350900994514873E-4	0.0	0.0	11.206564405287578	0.0
21	2.3350900994514873E-4	0.0	0.0	11.291328175897666	0.0
22	2.3350900994514873E-4	0.0	0.0	11.455952027908998	0.0
23	2.3350900994514873E-4	0.0	0.0	11.548655104857222	0.0
24	2.3350900994514873E-4	0.0	0.0	11.632251330417585	0.0
25	2.3350900994514873E-4	0.0	0.0	11.71141088478899	0.0
26	2.3350900994514873E-4	0.0	0.0	11.816022921244416	0.0
27	2.3350900994514873E-4	0.0	0.0	11.932543917207045	0.0
28	2.3350900994514873E-4	0.0	0.0	12.018241723856915	0.0
29	2.3350900994514873E-4	0.0	0.0	12.116549017043823	0.0
30	2.3350900994514873E-4	0.0	0.0	12.239141247265026	0.0
31	4.6701801989029745E-4	0.0	0.0	12.337448540451934	0.0
32	4.6701801989029745E-4	0.0	0.0	12.443228121957086	0.0
33	4.6701801989029745E-4	0.0	0.0	12.553210865641251	0.0
34	4.6701801989029745E-4	0.0	0.0	12.694950834677956	0.0
35	4.6701801989029745E-4	0.0	0.0	12.846965200152248	0.0
36	4.6701801989029745E-4	0.0	0.0	12.977496736711586	0.0
37	4.6701801989029745E-4	0.0	0.0	13.112464944459882	0.0
38	4.6701801989029745E-4	0.0	0.0	13.302541278555234	0.0
39	4.6701801989029745E-4	0.0	0.0	13.599564739205462	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACG	30	2.1623E-6	45.000004	7
GTTCGGT	30	2.1623E-6	45.000004	9
TAGGGCG	30	2.1623E-6	45.000004	5
CTACCGG	20	7.028322E-4	45.000004	2
ATACGGT	20	7.028322E-4	45.000004	6
AAGTGCG	20	7.028322E-4	45.000004	12
ACCGGTC	25	3.8866925E-5	45.0	17
TACGGTG	25	3.8866925E-5	45.0	7
ACGGCCT	25	3.8866925E-5	45.0	8
CGTTTTT	12695	0.0	44.432846	1
TGATACC	920	0.0	42.798912	4
GATACCT	920	0.0	42.798912	5
TTCGTTG	90	0.0	42.5	1
TTTCGCG	85	0.0	42.35294	1
TACGGCT	840	0.0	42.05357	7
CGGCTGT	845	0.0	41.538464	9
ACGGCTG	855	0.0	41.31579	8
TACCTGT	960	0.0	41.250004	7
CGTTTGG	110	0.0	40.909092	2
ATACCTG	965	0.0	40.80311	6
>>END_MODULE