##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933331.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413619
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.084135399969536	31.0	28.0	31.0	23.0	33.0
2	29.599455054047322	31.0	30.0	33.0	22.0	34.0
3	29.753449430514557	31.0	30.0	33.0	16.0	34.0
4	32.61841694893126	35.0	32.0	37.0	22.0	37.0
5	33.83501241480686	35.0	33.0	37.0	30.0	37.0
6	33.40445192314667	35.0	35.0	37.0	28.0	37.0
7	32.215507508117376	35.0	31.0	35.0	25.0	37.0
8	33.46588043586006	35.0	33.0	37.0	28.0	37.0
9	33.13351175840568	35.0	31.0	37.0	25.0	39.0
10	31.695734480282578	35.0	27.0	39.0	15.0	39.0
11	32.48843984439787	37.0	30.0	39.0	15.0	39.0
12	33.282898029345844	37.0	31.0	39.0	23.0	39.0
13	33.39123686291007	37.0	32.0	39.0	23.0	39.0
14	33.64596645705347	37.0	31.0	40.0	19.0	41.0
15	34.371494056124114	37.0	32.0	40.0	25.0	41.0
16	34.59113822140666	37.0	32.0	40.0	25.0	41.0
17	32.738421107347584	36.0	29.0	40.0	18.0	41.0
18	33.370696220434745	36.0	31.0	39.0	23.0	39.0
19	33.09227574168498	36.0	31.0	37.0	23.0	39.0
20	32.76828675665286	35.0	31.0	36.0	25.0	39.0
21	32.84043044444283	35.0	31.0	37.0	24.0	39.0
22	32.92587622909006	34.0	31.0	37.0	25.0	39.0
23	34.0953171880402	35.0	33.0	38.0	27.0	40.0
24	32.16798551323803	34.0	31.0	37.0	21.0	39.0
25	31.23061561485328	34.0	29.0	37.0	18.0	39.0
26	30.44692337634393	33.0	25.0	37.0	18.0	39.0
27	32.289865794366314	34.0	30.0	37.0	22.0	39.0
28	32.45474941915144	35.0	31.0	37.0	21.0	39.0
29	32.627550958732556	35.0	31.0	38.0	22.0	40.0
30	31.55226428186326	35.0	30.0	37.0	18.0	40.0
31	31.9663168278053	35.0	30.0	37.0	21.0	40.0
32	31.685304591907045	35.0	30.0	37.0	18.0	40.0
33	31.52667309770586	35.0	30.0	38.0	15.0	40.0
34	30.444894939545815	35.0	27.0	38.0	10.0	40.0
35	29.810103017511285	35.0	25.0	38.0	8.0	40.0
36	29.056568968059977	34.0	22.0	38.0	7.0	40.0
37	29.083559991199632	34.0	22.0	38.0	7.0	40.0
38	29.111958106373258	34.0	22.0	38.0	7.0	40.0
39	28.72581288577169	34.0	21.0	38.0	7.0	40.0
40	28.613939398335184	34.0	21.0	38.0	7.0	40.0
41	28.256716930315097	33.0	20.0	38.0	7.0	40.0
42	28.502711432501894	34.0	21.0	38.0	7.0	40.0
43	28.500595959083117	34.0	20.0	38.0	7.0	40.0
44	28.38618632122799	34.0	20.0	38.0	7.0	40.0
45	28.535613692794577	34.0	20.0	38.0	7.0	40.0
46	28.37352007523832	34.0	20.0	38.0	7.0	40.0
47	28.41709399229726	34.0	20.0	38.0	7.0	40.0
48	28.161051595792262	34.0	20.0	37.0	7.0	40.0
49	27.87813422497516	33.0	20.0	37.0	7.0	39.0
50	28.113839064453035	34.0	20.0	37.0	7.0	40.0
51	26.76284455017782	32.0	18.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	15.0
10	13.0
11	26.0
12	38.0
13	50.0
14	113.0
15	226.0
16	417.0
17	804.0
18	1339.0
19	2291.0
20	3516.0
21	4825.0
22	6822.0
23	9646.0
24	13503.0
25	18428.0
26	23242.0
27	25072.0
28	23491.0
29	23592.0
30	24868.0
31	27813.0
32	31083.0
33	35072.0
34	39556.0
35	43925.0
36	32472.0
37	17453.0
38	3834.0
39	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.126343325620923	2.465554048532587	35.84071331345997	43.56738931238652
2	44.21532859950824	7.105331234783701	34.70367657191763	13.975663593790422
3	12.61934292186771	7.115001970412384	65.94547155715767	14.320183550562232
4	11.108048711495362	2.8035462587550377	69.21248782091732	16.875917208832284
5	15.34455622203042	3.150242131043303	65.69910956701699	15.80609207990929
6	13.916430338064743	7.0415043796343975	67.12699368259194	11.915071599708911
7	53.69627604147779	1.0147019358395044	42.90276800630532	2.386254016377391
8	55.81005708151705	4.5121234759525075	37.35756819681881	2.320251245711633
9	51.96980796336725	2.5216443151789445	37.57443444329201	7.934113278161786
10	27.264946726335104	21.008464311359003	42.03143472616103	9.695154236144859
11	19.978772735295042	16.067927247055867	52.08053788631567	11.872762131333426
12	17.53570314709914	15.567466678271549	52.91149584521021	13.985334329419102
13	16.17793186483213	15.903524741368264	56.12290537910493	11.795638014694683
14	14.145385004073797	16.469262775646186	54.32028025791852	15.065071962361499
15	12.473314813874605	15.496145003010017	57.50050166941074	14.53003851370464
16	15.137360711185899	15.920932065499894	52.35325263104451	16.5884545922697
17	15.260904358842318	16.110720252212786	54.49411173084408	14.134263658100812
18	15.74758412935576	14.714024259040325	53.92353832875182	15.614853282852092
19	15.179186642779948	17.727909017719206	51.43018091528677	15.66272342421407
20	16.824178773218833	18.25979947729674	53.37013048240047	11.54589126708396
21	16.597641791116946	19.193992539027462	51.81701034043407	12.39135532942152
22	15.446098946131585	15.583423392058876	53.66412084551242	15.306356816297123
23	14.436232378106423	19.21696053614558	52.95332177680425	13.39348530894374
24	14.502476917162896	17.13775237597886	53.17623223304538	15.183538473812858
25	14.80565447912209	19.669792731958637	50.41427013749369	15.110282651425589
26	14.514565336698748	17.5690671850181	52.04669031161527	15.869677166667875
27	14.73820109811203	17.185138980559405	51.7848551444687	16.291804776859863
28	12.372739163336307	19.142737640195445	52.756522306760566	15.728000889707678
29	15.429900463953542	18.510996835251767	50.70318336440057	15.355919336394122
30	15.947768356869485	18.460225473201184	52.03726134437731	13.554744825552017
31	16.550013418145685	18.924662551768655	49.65705153776785	14.86827249231781
32	17.279670421329772	20.659834291945003	48.36890955202735	13.691585734697876
33	17.1836883702151	19.530775907296327	46.06703270401022	17.21850301847836
34	16.23112091078988	23.267306385828505	45.36856382322863	15.13300888015299
35	16.628104608347297	24.71960910886589	42.70959506212239	15.942691220664429
36	17.88046487226167	26.278048155428063	40.137421153283576	15.704065819026688
37	18.33305529968401	25.857371155580378	40.069484235492084	15.74008930924353
38	19.459454232034794	25.052765951273997	40.31004378425556	15.177736032435648
39	18.64396945014615	24.038547552215928	38.98031763531172	18.3371653623262
40	20.9630118539042	23.941598427538388	39.533725481663076	15.561664236894341
41	19.055459251146587	23.997446925794026	38.88965448879282	18.057439334266558
42	18.019965233705413	24.849680503071667	40.54335028129753	16.587003981925395
43	19.770368382496937	22.715590918212172	39.322419908176364	18.19162079111453
44	20.02398342435913	23.251591440431895	38.32488352807778	18.3995416071312
45	17.81881393262882	23.349507638672304	38.549002826272485	20.282675602426387
46	18.95149884313825	24.16934425159386	37.83167601101497	19.047480894252924
47	16.941919979498042	23.357727763956685	42.06141400660995	17.638938249935325
48	17.70880931485256	24.29337143603171	38.3210152338263	19.676804015289434
49	17.42545676093216	23.489975073678917	39.83206767580793	19.252500489580992
50	17.966534419356943	22.293705076410898	39.99574487632338	19.744015627908777
51	17.277736274204038	21.966834212161434	38.9558990278493	21.799530485785226
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55.0
1	388.0
2	721.0
3	23049.0
4	45377.0
5	30228.5
6	15080.0
7	14179.0
8	13278.0
9	12816.5
10	12355.0
11	11590.5
12	10826.0
13	9999.5
14	9173.0
15	8461.0
16	7749.0
17	7152.0
18	6555.0
19	6005.0
20	5455.0
21	5000.5
22	4546.0
23	4485.0
24	4424.0
25	4378.5
26	4756.5
27	5180.0
28	5630.0
29	6080.0
30	6680.5
31	7281.0
32	7838.0
33	8395.0
34	9319.5
35	10244.0
36	10746.5
37	11249.0
38	12151.0
39	13053.0
40	13463.5
41	13874.0
42	14619.5
43	15365.0
44	16163.0
45	16961.0
46	24002.0
47	31043.0
48	27379.0
49	23715.0
50	22898.0
51	22081.0
52	20571.0
53	19061.0
54	17794.5
55	16528.0
56	15177.0
57	13826.0
58	12818.5
59	11811.0
60	10257.0
61	8703.0
62	7554.0
63	6405.0
64	5384.5
65	4364.0
66	3606.0
67	2848.0
68	2359.5
69	1871.0
70	1548.5
71	1226.0
72	1044.5
73	863.0
74	802.5
75	608.5
76	475.0
77	373.5
78	272.0
79	182.5
80	93.0
81	67.0
82	41.0
83	31.0
84	21.0
85	15.5
86	10.0
87	6.5
88	3.0
89	4.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	413619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.700448062772864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.8568723781552	41.557925071576754
2	9.06181798430924	9.551237360727802
3	4.380568741297139	6.925738063084083
4	2.6194739621121275	5.52189805968304
5	1.6209844288150466	4.271330285066545
6	1.067765785854465	3.3763041204377444
7	0.7179277715514899	2.648458066623008
8	0.456182537229852	1.9232819288340615
9	0.3133309709628803	1.4861414305518719
>10	0.8071797189135066	6.599425247512067
>50	0.05901847282621228	2.1295680771027303
>100	0.03513004334889863	3.3095072690034653
>500	0.0018736023119412602	0.7166032372579293
>1k	0.0014052017339559453	2.538597207013622
>5k	0.0	0.0
>10k+	4.6840057798531505E-4	7.443984575525358
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30156	7.2907675904636875	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCG	4184	1.0115589467601827	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGC	3297	0.7971103841941498	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTC	2803	0.6776767991799216	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAAGCAGT	852	0.20598666889093584	No Hit
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	829	0.20042599590444346	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCT	716	0.17310616775341559	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	506	0.1223348057028328	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTC	494	0.11943358501422807	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	424	0.1025097976640338	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05705734020922636	0.0
2	0.0	0.0	0.0	0.3527400820561918	0.0
3	0.0	0.0	0.0	0.46637122569321043	0.0
4	0.0	0.0	0.0	0.8096823405114368	0.0
5	0.0	0.0	0.0	1.7666016309695638	0.0
6	0.0	0.0	0.0	2.1232100072772284	0.0
7	0.0	0.0	0.0	2.4648287433604357	0.0
8	0.0	0.0	0.0	2.875109702407288	0.0
9	0.0	0.0	0.0	3.1323512701302407	0.0
10	0.0	0.0	0.0	4.727539112081408	0.0
11	0.0	0.0	0.0	5.172634719391517	0.0
12	0.0	0.0	0.0	6.377124841943915	0.0
13	0.0	0.0	0.0	6.54370326314797	0.0
14	0.0	0.0	0.0	6.660719164255027	0.0
15	0.0	0.0	0.0	6.895476271641293	0.0
16	0.0	0.0	0.0	7.032558949177867	0.0
17	0.0	0.0	0.0	7.182213583031727	0.0
18	0.0	0.0	0.0	7.318087418614716	0.0
19	0.0	0.0	0.0	7.703949770199145	0.0
20	0.0	0.0	0.0	7.845626047159342	0.0
21	0.0	0.0	0.0	7.986818787338106	0.0
22	0.0	0.0	0.0	8.21190515909569	0.0
23	0.0	0.0	0.0	8.392264378570617	0.0
24	0.0	0.0	0.0	8.533698887140098	0.0
25	0.0	0.0	0.0	8.652407166982174	0.0
26	0.0	0.0	0.0	8.770873678433535	0.0
27	0.0	0.0	0.0	8.900945072639313	0.0
28	0.0	0.0	0.0	9.023521646732863	0.0
29	0.0	0.0	0.0	9.160120787488003	0.0
30	0.0	0.0	0.0	9.341688848916515	0.0
31	0.0	0.0	0.0	9.476112074155202	0.0
32	0.0	0.0	0.0	9.620689571804004	0.0
33	0.0	0.0	0.0	9.767201216578542	0.0
34	0.0	0.0	0.0	9.930878417093993	0.0
35	0.0	0.0	0.0	10.128161423919114	0.0
36	0.0	0.0	0.0	10.288212098573808	0.0
37	0.0	0.0	0.0	10.45503228816858	0.0
38	0.0	0.0	0.0	10.686404638084808	0.0
39	0.0	0.0	0.0	11.070574610934218	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	45	3.8380676E-10	45.000004	24
CCGGGTC	30	2.16212E-6	45.000004	5
TAATCGT	45	3.8380676E-10	45.000004	21
GGTTTCG	20	7.028044E-4	45.0	1
TTCGTAC	20	7.028044E-4	45.0	33
CGATTCC	20	7.028044E-4	45.0	10
TCGTACA	20	7.028044E-4	45.0	34
TTACGGG	25	3.8864608E-5	45.0	3
GGCGATA	20	7.028044E-4	45.0	8
ACCCCGA	20	7.028044E-4	45.0	39
GACTCAA	25	3.8864608E-5	45.0	9
CGTTTTT	13775	0.0	44.542652	1
GATACCT	605	0.0	42.39669	5
TGGGCGA	505	0.0	41.88119	6
CACGGGA	70	0.0	41.785713	4
TGATACC	615	0.0	41.707317	4
GCGATTC	65	0.0	41.53846	9
CACCGGT	60	3.6379788E-12	41.250004	16
GACACGA	280	0.0	40.98214	25
AGACACG	280	0.0	40.98214	24
>>END_MODULE