##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933330.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	753365
Sequences flagged as poor quality	0
Sequence length	51
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.732741765279776	31.0	28.0	33.0	25.0	34.0
2	29.2675104365082	31.0	30.0	33.0	16.0	34.0
3	29.188080147073464	31.0	28.0	34.0	16.0	34.0
4	33.5243261898283	35.0	32.0	37.0	28.0	37.0
5	34.461042124335485	35.0	35.0	37.0	32.0	37.0
6	34.10820120393169	35.0	35.0	37.0	28.0	37.0
7	34.04358179634042	35.0	33.0	37.0	30.0	37.0
8	33.99687402520691	35.0	35.0	37.0	28.0	37.0
9	35.23830015994903	37.0	34.0	39.0	30.0	39.0
10	35.221029646983865	37.0	34.0	39.0	28.0	39.0
11	35.60155966895197	37.0	34.0	39.0	30.0	39.0
12	35.72324304951783	37.0	35.0	39.0	30.0	39.0
13	35.94870082894746	37.0	35.0	39.0	31.0	39.0
14	36.72150551193644	39.0	36.0	41.0	30.0	41.0
15	37.124534588147846	39.0	36.0	41.0	31.0	41.0
16	37.020457547138506	39.0	36.0	41.0	31.0	41.0
17	36.23078852880078	38.0	34.0	41.0	29.0	41.0
18	35.883328798125746	38.0	34.0	39.0	29.0	40.0
19	35.039477544085535	37.0	34.0	37.0	27.0	40.0
20	34.16041228355445	35.0	33.0	37.0	27.0	40.0
21	34.27850377970837	35.0	33.0	38.0	29.0	40.0
22	34.57613241921246	35.0	33.0	38.0	29.0	40.0
23	34.96194938708329	35.0	33.0	39.0	30.0	40.0
24	34.27016917430462	35.0	33.0	38.0	27.0	40.0
25	33.282200526969	35.0	33.0	37.0	24.0	40.0
26	33.14345503175751	35.0	32.0	38.0	23.0	40.0
27	34.05928069395313	35.0	33.0	38.0	25.0	40.0
28	33.80372064006159	35.0	33.0	38.0	24.0	40.0
29	33.60038095743763	35.0	33.0	38.0	23.0	40.0
30	32.73626329866665	35.0	32.0	38.0	20.0	40.0
31	32.52315278782529	35.0	32.0	38.0	18.0	40.0
32	31.482823067171957	35.0	30.0	38.0	15.0	40.0
33	30.20590683135001	35.0	23.0	39.0	8.0	40.0
34	29.066906479594884	35.0	20.0	39.0	7.0	40.0
35	28.334662481001907	35.0	16.0	39.0	7.0	40.0
36	27.610467701578916	35.0	15.0	39.0	7.0	40.0
37	27.34618146582334	34.0	12.0	39.0	7.0	40.0
38	27.04201018098797	34.0	10.0	39.0	7.0	40.0
39	26.57831462836739	33.0	10.0	38.0	7.0	40.0
40	26.34220729659594	33.0	10.0	38.0	7.0	40.0
41	26.088289209081918	32.0	10.0	38.0	7.0	40.0
42	26.081842134954503	33.0	10.0	38.0	7.0	40.0
43	25.917914954902336	32.0	10.0	38.0	7.0	40.0
44	25.836808187266463	33.0	10.0	38.0	7.0	40.0
45	25.900291359434007	33.0	10.0	38.0	7.0	40.0
46	25.67333629781049	32.0	10.0	38.0	7.0	40.0
47	25.514239445687018	31.0	10.0	38.0	7.0	40.0
48	25.404274156617312	31.0	10.0	37.0	7.0	40.0
49	25.513573102015624	32.0	10.0	37.0	7.0	40.0
50	25.49337173879859	31.0	10.0	37.0	7.0	40.0
51	24.669857240514226	30.0	10.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	27.0
10	21.0
11	39.0
12	54.0
13	86.0
14	131.0
15	243.0
16	464.0
17	1020.0
18	2032.0
19	3754.0
20	6000.0
21	9195.0
22	13758.0
23	20436.0
24	30802.0
25	45155.0
26	56143.0
27	53447.0
28	44118.0
29	36297.0
30	32745.0
31	33162.0
32	35426.0
33	40833.0
34	46603.0
35	53476.0
36	57858.0
37	65579.0
38	55764.0
39	8687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.307951656899377	2.173448461237249	26.699674128742373	50.818925753121
2	49.366376192151215	11.469739103887227	26.854048170541507	12.309836533420055
3	9.67977009815959	11.608582825058239	65.85904574807697	12.85260132870521
4	8.282970406111248	2.2732672741632545	76.05078547583177	13.39297684389373
5	15.264712324039476	2.7638661206719184	68.55893225727237	13.412489298016233
6	10.926443357469488	9.826710824102527	70.44420699129904	8.802638827128948
7	44.04611310586502	0.8122224950721099	53.288512208557606	1.8531521905052664
8	43.85284689360403	6.704452688935642	47.22770503009829	2.214995387362036
9	41.1927817193525	2.1106634898090566	46.58392678183882	10.112628008999621
10	22.408925288538757	13.897911370982193	53.445275530453365	10.247887810025686
11	16.880263882712896	14.2977175738188	57.2442308841	11.577787659368301
12	12.36824115800442	13.011355717348163	60.50679285605251	14.113610268594904
13	12.71110285187127	13.423506534017376	64.25782986998334	9.607560744128012
14	12.00321225435214	17.260690369210145	58.620456219760676	12.115641156677043
15	10.18058975397052	14.20161541882089	64.17062114645623	11.447173680752359
16	11.317356128835291	15.354841278795803	58.00256183921473	15.325240753154182
17	11.604998904913288	15.490101079821866	60.69023647236067	12.21466354290417
18	11.99750452967685	14.598235914861988	59.891155017820054	13.513104537641116
19	11.743975363867449	16.309093201834436	57.54554565184207	14.401385782456048
20	13.404525031027456	15.670624464900811	60.795364796612525	10.1294857074592
21	13.105599543382027	18.08114260683732	58.65257876328207	10.160679086498577
22	12.163028545260266	12.957995128523358	60.186496585320526	14.692479740895848
23	11.973080777577934	17.574217013001668	59.54842606173635	10.904276147684056
24	13.143828024928156	15.102904966384155	58.118176448335134	13.63509056035255
25	11.011528276466255	20.649087759585328	56.63244244157878	11.706941522369634
26	12.040246095849954	14.586422252161968	59.2818885931786	14.091443058809475
27	14.136042953946626	15.702348795072773	56.74181837489132	13.41978987608928
28	11.165105891566505	15.894154891719154	59.37692884591135	13.563810370802997
29	16.755888579904827	13.867381680858548	56.218831509294965	13.157898229941662
30	14.42488037007294	17.01406356812435	56.58638243082702	11.974673630975689
31	14.537707485747282	15.156663768558401	53.92233512308111	16.38329362261321
32	16.84535384574542	18.330025950236607	51.89848214344972	12.92613806056825
33	13.80512766056294	20.213442355299225	48.085058371440134	17.896371612697695
34	17.21250655392804	22.564228494819908	45.90138910090062	14.321875850351423
35	16.080253263690242	24.409549156119546	42.84921651523498	16.660981064955234
36	17.063176547888474	24.440344321809484	40.20773463062393	18.28874449967811
37	19.353036044945014	23.825237434709603	41.516130959096856	15.30559556124853
38	17.37935794734292	26.19646519283482	39.129372880343524	17.29480397947874
39	20.553118342370563	24.79117028266511	38.5284689360403	16.12724243892403
40	18.553423639271802	26.81051017766952	39.62063541576792	15.015430767290756
41	17.567845599410646	28.487386592156522	37.766554060780635	16.1782137476522
42	17.4194447578531	26.223543700596657	40.20255785708123	16.15445368446902
43	19.650103203626397	24.59100170568051	37.961413126439375	17.797481964253713
44	19.977700052431423	24.395478951106035	36.723102347467695	18.903718648994843
45	17.33183782097655	23.6717925573925	37.62386094389837	21.372508677732576
46	20.638468736933625	24.55502976644787	36.61598295646865	18.19051854014986
47	16.87893650488143	23.441227028067406	41.81359633112767	17.86624013592349
48	18.25954218738593	24.850238596165205	36.481652319924606	20.40856689652426
49	17.100608602735726	23.45104962402023	40.119596742614796	19.328745030629243
50	17.910043604361764	22.76877741864833	39.02092611151301	20.300252865476896
51	17.99698685232258	22.596085562775016	37.12423592813576	22.28269165676664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	58.0
1	1152.0
2	2246.0
3	30582.0
4	58918.0
5	45071.0
6	31224.0
7	30281.0
8	29338.0
9	28946.5
10	28555.0
11	27842.5
12	27130.0
13	26244.5
14	25359.0
15	24275.5
16	23192.0
17	22015.5
18	20839.0
19	19460.0
20	18081.0
21	16941.5
22	15802.0
23	15002.0
24	14202.0
25	13424.0
26	12410.0
27	12174.0
28	12251.5
29	12329.0
30	12910.0
31	13491.0
32	14236.5
33	14982.0
34	15893.0
35	16804.0
36	17737.5
37	18671.0
38	19570.5
39	20470.0
40	21441.0
41	22412.0
42	23299.0
43	24186.0
44	25950.5
45	27715.0
46	54442.5
47	81170.0
48	59311.5
49	37453.0
50	34933.0
51	32413.0
52	29320.5
53	26228.0
54	24447.5
55	22667.0
56	20606.5
57	18546.0
58	16623.5
59	14701.0
60	12541.5
61	10382.0
62	8810.0
63	7238.0
64	5904.5
65	4571.0
66	3624.0
67	2677.0
68	2169.5
69	1662.0
70	1329.5
71	997.0
72	802.0
73	607.0
74	562.0
75	463.0
76	409.0
77	289.5
78	170.0
79	119.0
80	68.0
81	52.5
82	37.0
83	28.0
84	19.0
85	11.5
86	4.0
87	3.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	753365.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.493184390786865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.48810083284744	48.834848764523514
2	7.628222127817496	8.923980069926186
3	3.6445130385781206	6.395375195405309
4	1.990266484387411	4.656680978323038
5	1.1789918315925838	3.448149330028827
6	0.6688134423959156	2.347261680546072
7	0.41969921118746406	1.7184680344059295
8	0.24987888928305468	1.1692969556958988
9	0.1528360927460995	0.8045882779108501
>10	0.5037368495807384	4.899844527175434
>50	0.03792768841642182	1.5510844890841116
>100	0.03244394188777851	3.3996444470716995
>500	0.002056306175793197	0.8486414905934627
>1k	0.0015993492478391534	2.1725222159192645
>5k	2.284784639770219E-4	1.3288255655072125
>10k+	6.854353919310657E-4	7.500787977883165
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28673	3.8059904561533915	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	14703	1.9516436255998089	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	12749	1.6922739973319705	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	9943	1.3198117778234986	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT	3384	0.44918465816702396	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTC	2549	0.3383486092398771	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	2376	0.31538497275557	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCGAAAAT	2275	0.3019784566577954	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT	2076	0.2755636378116849	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT	1829	0.24277740537455286	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTA	1767	0.23454766281948325	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGT	903	0.11986221818109416	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	900	0.11946400483165531	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	782	0.10380094642039384	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3273778314628368E-4	0.0	0.0	0.036104677015789156	0.0
2	1.3273778314628368E-4	0.0	0.0	0.4138764078501125	0.0
3	1.3273778314628368E-4	0.0	0.0	0.4886077797614702	0.0
4	1.3273778314628368E-4	0.0	0.0	1.0447790911443988	0.0
5	1.3273778314628368E-4	0.0	0.0	3.393043212785303	0.0
6	1.3273778314628368E-4	0.0	0.0	3.7713458947522116	0.0
7	1.3273778314628368E-4	0.0	0.0	4.186947893783226	0.0
8	1.3273778314628368E-4	0.0	0.0	4.676219362460428	0.0
9	1.3273778314628368E-4	0.0	0.0	4.785329820206672	0.0
10	1.3273778314628368E-4	0.0	0.0	6.865463619892084	0.0
11	1.3273778314628368E-4	0.0	0.0	6.99992699421927	0.0
12	1.3273778314628368E-4	0.0	0.0	8.664989746006253	0.0
13	1.3273778314628368E-4	0.0	0.0	8.801576924863777	0.0
14	1.3273778314628368E-4	0.0	0.0	8.908829053645976	0.0
15	1.3273778314628368E-4	0.0	0.0	9.226072355365593	0.0
16	1.3273778314628368E-4	0.0	0.0	9.415223696349047	0.0
17	1.3273778314628368E-4	0.0	0.0	9.514511558142468	0.0
18	1.3273778314628368E-4	0.0	0.0	9.586322698824606	0.0
19	1.3273778314628368E-4	0.0	0.0	10.023295480942172	0.0
20	1.3273778314628368E-4	0.0	0.0	10.113158960132207	0.0
21	1.3273778314628368E-4	0.0	0.0	10.182979034067152	0.0
22	1.3273778314628368E-4	0.0	0.0	10.320097164057263	0.0
23	1.3273778314628368E-4	0.0	0.0	10.39841245611357	0.0
24	1.3273778314628368E-4	0.0	0.0	10.473276565808074	0.0
25	1.3273778314628368E-4	0.0	0.0	10.52915917251266	0.0
26	1.3273778314628368E-4	0.0	0.0	10.612916713677965	0.0
27	1.3273778314628368E-4	0.0	0.0	10.717912300146676	0.0
28	1.3273778314628368E-4	0.0	0.0	10.783617502804086	0.0
29	1.3273778314628368E-4	0.0	0.0	10.857286972450273	0.0
30	1.3273778314628368E-4	0.0	0.0	10.941973678097602	0.0
31	1.3273778314628368E-4	0.0	0.0	11.023209201383128	0.0
32	1.3273778314628368E-4	0.0	0.0	11.10776316924731	0.0
33	1.3273778314628368E-4	0.0	0.0	11.190193332581153	0.0
34	1.3273778314628368E-4	0.0	0.0	11.306471630617297	0.0
35	1.3273778314628368E-4	0.0	0.0	11.406290443543302	0.0
36	1.3273778314628368E-4	0.0	0.0	11.498012251697384	0.0
37	1.3273778314628368E-4	0.0	0.0	11.601945935900925	0.0
38	1.3273778314628368E-4	0.0	0.0	11.71636590497302	0.0
39	1.3273778314628368E-4	0.0	0.0	11.841670372263113	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	25	3.8895218E-5	45.0	2
TACGCCT	35	1.2111741E-7	45.0	7
CGAGGGT	20	7.0317386E-4	45.0	4
CGCACGG	20	7.0317386E-4	45.0	2
GCCGTTG	20	7.0317386E-4	45.0	1
CGTTTTT	29435	0.0	44.510788	1
TACGGCT	1150	0.0	42.847824	7
ACGGCTG	1155	0.0	42.662334	8
GATACCT	1570	0.0	42.27707	5
GCGACCT	165	0.0	42.272728	11
CGATGAA	395	0.0	42.1519	19
TGATACC	1595	0.0	42.037617	4
CGCTTTT	740	0.0	41.959457	1
CGGCTGT	1195	0.0	41.422596	9
CCGATGA	420	0.0	40.714287	18
AATCTGT	1805	0.0	40.63712	2
ATACCTG	1645	0.0	40.34954	6
CGGTAGT	95	0.0	40.263157	12
GAATCTG	1835	0.0	39.97275	1
TACCTGT	1675	0.0	39.895523	7
>>END_MODULE