##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933329.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483624
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.005487734272908	31.0	28.0	31.0	23.0	33.0
2	29.44877011893537	31.0	30.0	33.0	16.0	34.0
3	29.567254726812564	31.0	30.0	33.0	16.0	34.0
4	32.643439945081305	35.0	32.0	37.0	19.0	37.0
5	33.90712206176699	35.0	33.0	37.0	30.0	37.0
6	33.38670330670107	35.0	35.0	37.0	28.0	37.0
7	32.669627644616476	35.0	32.0	37.0	26.0	37.0
8	33.507013713132515	35.0	33.0	37.0	28.0	37.0
9	33.60242461085471	35.0	32.0	39.0	26.0	39.0
10	32.79984037185913	37.0	31.0	39.0	16.0	39.0
11	33.63698451689743	37.0	31.0	39.0	21.0	39.0
12	34.217540899541795	37.0	32.0	39.0	26.0	39.0
13	34.45533720410898	37.0	32.0	39.0	27.0	39.0
14	34.56760830728003	37.0	32.0	40.0	24.0	41.0
15	35.24756629116835	38.0	32.0	40.0	27.0	41.0
16	35.36961358410666	38.0	33.0	40.0	27.0	41.0
17	33.481673779630455	37.0	31.0	40.0	19.0	41.0
18	33.96080839660563	37.0	32.0	39.0	25.0	39.0
19	33.59132921443105	37.0	32.0	37.0	25.0	39.0
20	32.898559624832515	35.0	32.0	36.0	25.0	39.0
21	32.9936851769143	35.0	32.0	37.0	25.0	39.0
22	32.72063627942369	34.0	30.0	37.0	25.0	39.0
23	33.89343580963724	35.0	33.0	38.0	27.0	40.0
24	32.004360825765474	35.0	31.0	36.0	21.0	39.0
25	31.16993159975518	34.0	28.0	36.0	18.0	39.0
26	29.877944436173557	33.0	24.0	36.0	18.0	39.0
27	32.08864531123352	34.0	30.0	37.0	22.0	39.0
28	32.25925925925926	35.0	31.0	37.0	21.0	39.0
29	32.105342166641854	35.0	30.0	38.0	21.0	40.0
30	31.142255140356973	34.0	27.0	37.0	18.0	40.0
31	31.564326832415265	35.0	30.0	37.0	20.0	39.0
32	31.121540701040477	35.0	30.0	37.0	16.0	39.0
33	30.322196582469026	35.0	25.0	38.0	12.0	40.0
34	28.709882057135296	34.0	21.0	37.0	7.0	40.0
35	27.862477875374257	33.0	18.0	37.0	7.0	40.0
36	27.114289199874282	33.0	15.0	37.0	7.0	40.0
37	27.048868542504096	33.0	15.0	38.0	7.0	40.0
38	26.9845334392007	33.0	15.0	38.0	7.0	40.0
39	26.545233487171853	33.0	13.0	37.0	7.0	40.0
40	26.39352058624055	32.0	13.0	37.0	7.0	40.0
41	26.095429507220484	32.0	12.0	37.0	7.0	40.0
42	26.288703620994823	32.0	12.0	37.0	7.0	40.0
43	26.23704572146957	32.0	12.0	37.0	7.0	40.0
44	26.070579623840008	32.0	10.0	37.0	7.0	40.0
45	26.196549385473013	33.0	10.0	37.0	7.0	40.0
46	25.948118786495293	32.0	10.0	37.0	7.0	40.0
47	25.909177377466794	32.0	10.0	37.0	7.0	40.0
48	25.65883413560948	31.0	10.0	36.0	7.0	39.0
49	25.357668353927846	31.0	10.0	36.0	7.0	39.0
50	25.551163713959603	31.0	10.0	36.0	7.0	39.0
51	24.433142275817577	29.0	10.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	11.0
10	14.0
11	17.0
12	30.0
13	53.0
14	107.0
15	251.0
16	537.0
17	1019.0
18	1910.0
19	3177.0
20	5198.0
21	7713.0
22	10826.0
23	15350.0
24	21122.0
25	28876.0
26	34447.0
27	33008.0
28	28848.0
29	26234.0
30	26802.0
31	29542.0
32	32605.0
33	36315.0
34	40274.0
35	44271.0
36	32928.0
37	18218.0
38	3832.0
39	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.587836831919013	2.190751492895307	28.864572477792667	51.356839197393015
2	51.49682397895886	8.0140770515938	28.0991017815493	12.389997187898036
3	10.323722561328635	7.97375647196996	69.10947347526178	12.593047491439629
4	8.954890576150067	2.3896663523729176	74.81287115610475	13.842571915372273
5	13.676120291796934	2.759375051693051	70.31412833110019	13.250376325409821
6	11.493846459232792	7.216349891651365	71.46543595851323	9.824367690602617
7	45.39745752898946	0.8359800175342827	51.72075827502357	2.0458041784526824
8	46.63292144310456	4.781813971184225	46.15817246455924	2.4270921211519694
9	43.29437745025061	2.1613898400410236	46.1935305113063	8.350702198402065
10	21.84734421782211	15.306519114022462	52.52365474004599	10.322481928109442
11	15.821795444394818	14.976303905513374	57.833358145997714	11.368542504094089
12	12.525019436587101	13.634559078953897	60.440342083932975	13.40007940052603
13	12.500413544406399	14.104138752419235	63.29917456536482	10.096273137809538
14	11.90739086563115	15.40763072138686	59.688725125303954	12.99625328767803
15	10.48645228524639	14.688063454253717	62.98963657717566	11.835847683324236
16	11.369783137313284	14.596463368236481	58.80828908408184	15.225464410368387
17	11.711991133607928	15.026756323093974	60.69405157725837	12.567200966039731
18	11.952467205928572	14.280515441748134	60.075802689692814	13.691214662630474
19	11.885473012092039	16.479951367177808	57.84886606123766	13.7857095594925
20	13.230112649496304	15.884240649760972	60.22405835938663	10.661588341356094
21	13.36451458157577	17.257208079003522	58.27068135576398	11.107595983656724
22	12.336649959472648	14.037764875192298	59.50635204208228	14.119233123252775
23	11.88774750632723	17.61264949630291	58.60131010867947	11.898292888690387
24	12.199353216548394	15.483722892164161	58.6718194299704	13.645104461317056
25	11.3662680098589	18.396729690834203	57.12082113377335	13.116181165533556
26	12.175987957586885	15.755835111574282	58.55044414669247	13.51773278414636
27	12.787826906853258	16.042214613005147	57.50603774833342	13.663920731808183
28	11.518865895819893	16.094527980414536	59.134782392933346	13.251823730832216
29	15.421070914594809	14.338618430847106	56.71244603245496	13.527864622103122
30	13.816518617769177	16.468165351595452	57.17189386796354	12.543422162671828
31	14.058648867715414	15.79408796916613	55.21500173688651	14.93226142623195
32	15.13365755214795	17.927150017368866	53.95079648652672	12.988395943956462
33	13.402974211370816	19.109266703058577	50.91041801068599	16.57734107488462
34	16.264287959241063	21.629199543446976	47.67877524688601	14.42773725042595
35	15.372272674639802	23.44755429838056	44.454783054604405	16.725389972375236
36	16.45886060245149	22.957710949001704	42.32378872843366	18.259639720113146
37	18.045216945395598	23.4121962516335	42.87318247233388	15.669404330637024
38	16.74668750930475	25.11289762294675	41.99171257009578	16.148702297652722
39	18.801589664698195	24.409458587663142	41.007476882867685	15.781474864770978
40	17.909987924503334	25.6798670041189	41.743379154053564	14.6667659173242
41	16.856070004797115	26.511504805386004	40.91773774667924	15.714687443137645
42	16.911484955254497	25.076505715183693	42.21854167700528	15.793467652556531
43	18.432294509784462	23.709121135427523	40.87514267282021	16.983441681967808
44	18.89773873918581	23.544737233884174	39.784626073147734	17.772897953782277
45	16.94353464675037	22.958951582220898	40.120010586736804	19.97750318429193
46	18.827229417894895	23.848072055977372	39.086769887350506	18.23792863877723
47	16.458447058045092	22.914288786329877	42.841132780838	17.786131374787026
48	17.4906538964154	23.572444709112865	39.20049459911005	19.736406795361685
49	16.38587001472218	23.00340760590872	40.920012240914424	19.690710138454666
50	17.052296825633135	22.0762410467636	40.859427985377074	20.01203414222619
51	17.26072320645791	21.800406927695896	39.70398491390005	21.23488495194614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47.0
1	343.0
2	639.0
3	25823.0
4	51007.0
5	35300.5
6	19594.0
7	18825.0
8	18056.0
9	17805.0
10	17554.0
11	17298.0
12	17042.0
13	16259.5
14	15477.0
15	14763.5
16	14050.0
17	13122.5
18	12195.0
19	11407.5
20	10620.0
21	9847.0
22	9074.0
23	8529.5
24	7985.0
25	7649.0
26	7296.5
27	7280.0
28	7299.5
29	7319.0
30	7767.5
31	8216.0
32	8819.0
33	9422.0
34	10166.0
35	10910.0
36	11711.0
37	12512.0
38	13083.0
39	13654.0
40	14324.5
41	14995.0
42	15850.0
43	16705.0
44	17734.0
45	18763.0
46	28639.5
47	38516.0
48	33093.0
49	27670.0
50	25668.5
51	23667.0
52	21107.5
53	18548.0
54	16607.0
55	14666.0
56	13179.5
57	11693.0
58	10488.0
59	9283.0
60	7856.5
61	6430.0
62	5544.0
63	4658.0
64	3764.5
65	2871.0
66	2359.5
67	1848.0
68	1505.0
69	1162.0
70	939.0
71	716.0
72	590.5
73	465.0
74	443.0
75	369.0
76	317.0
77	234.5
78	152.0
79	107.5
80	63.0
81	44.0
82	25.0
83	19.5
84	14.0
85	9.5
86	5.0
87	4.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	483624.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.09301559105234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.12056483394528	49.11844833669406
2	8.474125557987994	10.015232674374392
3	3.51845369292843	6.237481167978462
4	1.9195533173203918	4.537287764330806
5	1.0681651841615196	3.156055094073799
6	0.6276386821376281	2.225343745746384
7	0.36459148954426596	1.5081367403203016
8	0.2226640753053455	1.0526313338868827
9	0.15106952763601103	0.8034438556734943
>10	0.45484311795310267	4.516512440874096
>50	0.04201364315023368	1.7404238010091637
>100	0.03204430405571385	3.704291756524944
>500	0.0021362869370475904	0.8350752005934058
>1k	0.0014241912913650603	2.1488090258655714
>5k	3.560478228412651E-4	1.1868629898078615
>10k+	3.560478228412651E-4	7.213964072246437
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34287	7.089598531090269	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	5641	1.1664019982465716	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	3963	0.8194382412783484	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	3776	0.780771839280102	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT	1367	0.2826576017732784	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	1107	0.22889682894149174	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCGAAAAT	993	0.20532479777678525	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTC	753	0.15569946900898218	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	584	0.12075496666832085	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTA	574	0.11868724463632906	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT	543	0.11227730633715449	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC	522	0.1079350900699717	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	495	0.10235224058359386	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06079102774055878	0.0
2	0.0	0.0	0.0	0.42264238333912296	0.0
3	0.0	0.0	0.0	0.5427770333978462	0.0
4	0.0	0.0	0.0	1.0466808925942468	0.0
5	0.0	0.0	0.0	2.2403768216631104	0.0
6	0.0	0.0	0.0	2.6795609812581676	0.0
7	0.0	0.0	0.0	3.06457082361504	0.0
8	0.0	0.0	0.0	3.536838535721966	0.0
9	0.0	0.0	0.0	3.7942699287049444	0.0
10	0.0	0.0	0.0	5.4881478173126235	0.0
11	0.0	0.0	0.0	5.905207351165368	0.0
12	0.0	0.0	0.0	7.133227465965295	0.0
13	0.0	0.0	0.0	7.3046416224174155	0.0
14	0.0	0.0	0.0	7.415471523332175	0.0
15	0.0	0.0	0.0	7.657601773278414	0.0
16	0.0	0.0	0.0	7.820951813805767	0.0
17	0.0	0.0	0.0	7.95866210113642	0.0
18	0.0	0.0	0.0	8.086654094916712	0.0
19	0.0	0.0	0.0	8.476212925743967	0.0
20	0.0	0.0	0.0	8.605859097149851	0.0
21	0.0	0.0	0.0	8.743776156683705	0.0
22	0.0	0.0	0.0	8.936901394471738	0.0
23	0.0	0.0	0.0	9.089706052635933	0.0
24	0.0	0.0	0.0	9.21356260235224	0.0
25	0.0	0.0	0.0	9.320463831406217	0.0
26	0.0	0.0	0.0	9.426331199444196	0.0
27	0.0	0.0	0.0	9.544811671877326	0.0
28	0.0	0.0	0.0	9.655228028385688	0.0
29	0.0	0.0	0.0	9.770400145567631	0.0
30	0.0	0.0	0.0	9.902734355615106	0.0
31	0.0	0.0	0.0	10.029278943973004	0.0
32	0.0	0.0	0.0	10.150860999454121	0.0
33	0.0	0.0	0.0	10.277612360015219	0.0
34	0.0	0.0	0.0	10.400435048715531	0.0
35	0.0	0.0	0.0	10.540626602484576	0.0
36	0.0	0.0	0.0	10.66779150745207	0.0
37	0.0	0.0	0.0	10.800332489702745	0.0
38	0.0	0.0	0.0	10.947967842786959	0.0
39	0.0	0.0	0.0	11.13654409210461	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	40	6.8012014E-9	45.0	1
ATCCGTC	35	1.2099736E-7	45.0	6
CACCCGT	35	1.2099736E-7	45.0	16
ACCGACT	30	2.162884E-6	44.999996	17
ATCCGGC	30	2.162884E-6	44.999996	6
GCGATCG	30	2.162884E-6	44.999996	9
CGTTTTT	20270	0.0	44.555992	1
TACGGCT	630	0.0	41.428574	7
ACCGGTG	60	3.6379788E-12	41.249996	17
ACGGCTG	635	0.0	41.102364	8
GATACCT	800	0.0	41.0625	5
CGACCAC	570	0.0	41.05263	12
CACGTGA	170	0.0	41.029415	43
TCGGGAA	110	0.0	40.909092	5
AGACACG	165	0.0	40.90909	24
CGTGAGG	160	0.0	40.78125	45
GAATGAT	2305	0.0	40.41215	1
CCGATGA	95	0.0	40.263157	18
GTTTTTT	22560	0.0	40.202793	2
CGGCTGT	605	0.0	40.16529	9
>>END_MODULE