##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933328.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637128
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.701130699011816	31.0	28.0	33.0	25.0	34.0
2	29.11779108750518	31.0	28.0	33.0	16.0	34.0
3	29.09264386434123	31.0	28.0	34.0	16.0	34.0
4	33.37750342160445	35.0	32.0	37.0	28.0	37.0
5	34.37636707223666	35.0	33.0	37.0	32.0	37.0
6	33.967537135395084	35.0	33.0	37.0	28.0	37.0
7	33.72734835072387	35.0	33.0	37.0	29.0	37.0
8	33.79901212943082	35.0	33.0	37.0	28.0	37.0
9	34.89976111550583	37.0	34.0	39.0	28.0	39.0
10	34.742034567622206	37.0	33.0	39.0	27.0	39.0
11	35.13780590399417	37.0	34.0	39.0	28.0	39.0
12	35.243659044964275	37.0	34.0	39.0	30.0	39.0
13	35.53762352305973	37.0	34.0	39.0	30.0	39.0
14	36.33481655177609	38.0	35.0	40.0	30.0	41.0
15	36.641748596828265	38.0	35.0	40.0	30.0	41.0
16	36.545264059969114	38.0	34.0	40.0	30.0	41.0
17	35.837917969387625	38.0	34.0	40.0	27.0	41.0
18	35.59751258773747	38.0	34.0	39.0	28.0	40.0
19	34.88056873971949	37.0	34.0	38.0	27.0	40.0
20	34.285754196958855	35.0	33.0	38.0	27.0	40.0
21	34.57885071759521	35.0	33.0	38.0	29.0	40.0
22	35.07438065820369	35.0	33.0	39.0	30.0	40.0
23	35.4236009090795	35.0	34.0	39.0	30.0	40.0
24	34.84301113747944	35.0	34.0	39.0	29.0	40.0
25	33.77210544819879	35.0	33.0	38.0	24.0	40.0
26	33.83715517133135	35.0	33.0	38.0	25.0	40.0
27	34.62300197134642	35.0	33.0	39.0	27.0	40.0
28	34.428780402054215	35.0	33.0	39.0	25.0	40.0
29	34.385928730176666	35.0	33.0	39.0	25.0	40.0
30	33.57399768963222	35.0	33.0	39.0	22.0	40.0
31	33.504710199520346	35.0	33.0	39.0	22.0	40.0
32	32.88571370274105	35.0	33.0	39.0	18.0	40.0
33	32.04714908150324	35.0	31.0	39.0	12.0	40.0
34	31.476868070466217	35.0	30.0	40.0	9.0	41.0
35	31.082810675405884	36.0	27.0	40.0	8.0	41.0
36	30.414010999359625	35.0	23.0	40.0	7.0	41.0
37	30.391842769427807	36.0	23.0	40.0	7.0	41.0
38	29.96038472645999	35.0	22.0	39.0	7.0	41.0
39	29.708104180007783	35.0	21.0	39.0	7.0	40.0
40	29.50711473989528	35.0	21.0	39.0	7.0	40.0
41	29.25615731846662	35.0	20.0	39.0	7.0	40.0
42	29.409795205986867	35.0	20.0	39.0	7.0	40.0
43	29.293496754184403	35.0	19.0	39.0	7.0	40.0
44	29.323981994198967	35.0	18.0	39.0	7.0	40.0
45	29.433529840157707	35.0	20.0	39.0	7.0	40.0
46	29.21683868861516	35.0	20.0	39.0	7.0	40.0
47	29.11222234778569	35.0	20.0	39.0	7.0	40.0
48	28.929750379829485	35.0	20.0	39.0	7.0	40.0
49	29.114761868886628	35.0	20.0	39.0	7.0	40.0
50	29.109623184038373	35.0	20.0	39.0	7.0	40.0
51	28.092472784118733	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	7.0
9	17.0
10	18.0
11	21.0
12	36.0
13	46.0
14	93.0
15	137.0
16	272.0
17	616.0
18	1285.0
19	2246.0
20	3657.0
21	5565.0
22	8178.0
23	12208.0
24	17799.0
25	26168.0
26	33285.0
27	33992.0
28	29703.0
29	26223.0
30	25822.0
31	27656.0
32	32248.0
33	38875.0
34	45953.0
35	56386.0
36	65421.0
37	73080.0
38	60415.0
39	9698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.29123818133876	2.4745420072575683	34.594775304177496	39.63944450722618
2	39.02418352356199	13.806487864291006	33.49107243756357	13.678256174583442
3	11.19916249168142	14.016178852601048	60.46210494594493	14.322553709772604
4	10.323514270287918	2.5826835423965044	71.07975163546415	16.014050551851433
5	18.67866425584812	3.077874461646639	61.54917065330672	16.694290629198527
6	13.350849436847856	11.852249469494355	64.19824587837923	10.598655215278562
7	53.99087781419119	0.8688991850931053	42.89499127333911	2.2452317273766025
8	53.79327230948883	8.186737986715386	35.494594492786376	2.5253952110094047
9	50.832956642935166	2.4389133737647692	34.542038648434854	12.186091334865209
10	28.27626473801183	18.292870506397456	41.4513253223842	11.979539433206513
11	19.765416054544772	16.547852236913148	49.75185520021095	13.934876508331136
12	15.296769252018432	15.233202747328637	52.57624841476124	16.89377958589169
13	15.131339385492398	15.632023706382391	57.334162052209294	11.902474855915923
14	14.526280433445086	20.243498951545057	49.90096181614997	15.329258798859884
15	11.999943496440276	16.615656508582262	57.26855514119612	14.115844853781342
16	13.482220213206766	18.14925729209829	49.30736053038008	19.061161964314863
17	14.103445461508521	17.758284049672906	52.755804171218344	15.382466317600233
18	14.119611757762963	17.739135621099685	51.5635790610364	16.57767356010095
19	13.999384739016335	19.372245451463442	48.99423663690812	17.634133172612096
20	16.017974410165618	18.46143945957484	53.22274331060635	12.297842819653193
21	16.291859720495726	21.55689280646903	49.683737019876695	12.46751045315855
22	14.904697329265076	15.42044926608154	51.68710212076694	17.987751283886443
23	14.710230911214072	20.791583480870408	50.97311686191786	13.525068745997665
24	15.968062932409186	17.6134152007132	49.1252935046019	17.293228362275713
25	13.534172097286573	24.25179869665122	47.52938185105662	14.684647355005588
26	14.140800592659561	17.80081867379867	50.791206790472245	17.267173943069526
27	16.878555015632653	19.0118783038887	47.42390853957132	16.68565814090732
28	12.68567697542723	19.84781707914265	51.31794553056842	16.14856041486169
29	17.045868334149496	19.298319960824198	47.77752665084567	15.878285054180635
30	16.68801245589583	21.082262904785225	48.82990545071006	13.399819188608882
31	17.26984216672317	20.723151391871024	45.14116472671111	16.865841714694692
32	20.113383809846688	21.38863776195678	44.50141886716641	13.996559561030123
33	16.430607350485303	19.47614921962306	44.70577340816916	19.387470021722482
34	20.32354566115443	23.746876608781907	40.63202370638239	15.29755402368127
35	18.583549930312277	21.842235783076553	39.54276063836466	20.031453648246504
36	16.812477241621778	26.30397659496993	39.924159666503435	16.95938649690486
37	21.405431875541492	23.277426200072824	39.34531208799488	15.971829836390805
38	17.77429339159478	25.318146432114112	39.006604638314435	17.90095553797667
39	23.916073379289564	23.410994337087683	36.39033286874851	16.282599414874248
40	18.444331437324994	23.45572632186939	39.253807712108085	18.84613452869753
41	19.89192124659409	25.624207380620533	37.79005160658455	16.693819766200825
42	17.927323865847995	24.046188520987933	38.970505141823935	19.055982471340137
43	19.782681031127183	23.525884908527015	37.64832184427619	19.043112216069613
44	20.80994713778079	23.72270564156653	35.631301716452576	19.836045504200097
45	17.52065519016587	23.96253186172951	36.177188885121986	22.339624062982637
46	22.029168393164326	24.92591755502819	34.72677389786668	18.31814015394081
47	16.872904659660225	22.970894388568702	41.94337715498298	18.212823796788086
48	20.03600532389096	24.72501600934192	34.32387212616617	20.91510654060095
49	17.572764028578245	22.876093971697994	39.06624728469005	20.484894715033715
50	18.63079318441506	22.234621614495044	38.290108110144274	20.844477090945617
51	18.702992177396066	22.816922188320085	35.43887570472496	23.041209929558896
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41.0
1	812.5
2	1584.0
3	20813.0
4	40042.0
5	30526.5
6	21011.0
7	20148.0
8	19285.0
9	18958.5
10	18632.0
11	17857.5
12	17083.0
13	16320.5
14	15558.0
15	14637.0
16	13716.0
17	12741.0
18	11766.0
19	10987.5
20	10209.0
21	9548.5
22	8888.0
23	8610.0
24	8332.0
25	8239.0
26	8333.0
27	8520.0
28	9033.5
29	9547.0
30	10598.5
31	11650.0
32	12632.5
33	13615.0
34	14811.5
35	16008.0
36	17716.0
37	19424.0
38	20118.5
39	20813.0
40	21549.5
41	22286.0
42	23481.5
43	24677.0
44	26484.5
45	28292.0
46	56261.5
47	84231.0
48	61017.0
49	37803.0
50	34760.5
51	31718.0
52	28235.0
53	24752.0
54	23193.0
55	21634.0
56	19870.0
57	18106.0
58	16363.5
59	14621.0
60	13001.0
61	11381.0
62	9891.0
63	8401.0
64	6937.0
65	5473.0
66	4549.0
67	3625.0
68	2990.5
69	2356.0
70	1915.5
71	1475.0
72	1233.0
73	991.0
74	833.5
75	535.0
76	394.0
77	290.5
78	187.0
79	151.5
80	116.0
81	75.5
82	35.0
83	24.5
84	14.0
85	9.5
86	5.0
87	5.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	637128.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.45380150228122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16662500092136	41.24877609905223
2	7.432711344964795	7.648825083351444
3	3.9994468864078065	6.173602386364319
4	2.599578026197773	5.3503268699868896
5	1.735233935668502	4.464219124295467
6	1.199307370738079	3.702535403650795
7	0.8321087722622473	2.997061171740203
8	0.5692376802253459	2.3431554084747184
9	0.38531212412886406	1.7843196196214026
>10	1.0026046996138533	7.616142202173802
>50	0.04787418856931947	1.6998420545252177
>100	0.024709253857393407	2.3929055745226893
>500	0.001853194039304384	0.5907172757189689
>1k	0.002162059712521781	2.6379650416085996
>5k	0.0	0.0
>10k+	0.0012354626928695893	9.34960668491328
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18985	2.9797780037920165	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	15036	2.3599653444833693	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	13917	2.184333446340453	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	10893	1.7097035446566469	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT	3415	0.535999045717658	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTC	2611	0.4098077623334715	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	2541	0.3988209590537537	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGTGCTT	2407	0.3777890784897226	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCT	2235	0.3507929332881305	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTA	1906	0.29915495787345714	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTT	1484	0.23292022953001595	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGT	961	0.15083311359726775	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04426112178400573	0.0
2	0.0	0.0	0.0	0.5124558958325486	0.0
3	0.0	0.0	0.0	0.6058437237101493	0.0
4	0.0	0.0	0.0	1.2663075551537524	0.0
5	0.0	0.0	0.0	4.084893459399053	0.0
6	0.0	0.0	0.0	4.448870556622845	0.0
7	0.0	0.0	0.0	4.9388819828982555	0.0
8	0.0	0.0	0.0	5.505173214801421	0.0
9	0.0	0.0	0.0	5.618651197247649	0.0
10	0.0	0.0	0.0	8.325956479702665	0.0
11	0.0	0.0	0.0	8.487619442247084	0.0
12	0.0	0.0	0.0	10.639620296078652	0.0
13	0.0	0.0	0.0	10.797673308974021	0.0
14	0.0	0.0	0.0	10.925591090016448	0.0
15	0.0	0.0	0.0	11.333201491693977	0.0
16	0.0	0.0	0.0	11.569574716540476	0.0
17	0.0	0.0	0.0	11.693411684936152	0.0
18	0.0	0.0	0.0	11.790252508130234	0.0
19	0.0	0.0	0.0	12.357485466028804	0.0
20	0.0	0.0	0.0	12.48430456674326	0.0
21	0.0	0.0	0.0	12.594957371203275	0.0
22	0.0	0.0	0.0	12.783145615951582	0.0
23	0.0	0.0	0.0	12.898664004721187	0.0
24	0.0	0.0	0.0	13.001469092552831	0.0
25	0.0	0.0	0.0	13.084968797478686	0.0
26	0.0	0.0	0.0	13.179769214349394	0.0
27	0.0	0.0	0.0	13.317575118343568	0.0
28	0.0	0.0	0.0	13.41504375886792	0.0
29	0.0	0.0	0.0	13.520203161688075	0.0
30	0.0	0.0	0.0	13.635407641792543	0.0
31	0.0	0.0	0.0	13.74904257857134	0.0
32	0.0	0.0	0.0	13.874135181627553	0.0
33	0.0	0.0	0.0	13.98824098140405	0.0
34	0.0	0.0	0.0	14.133109830363757	0.0
35	0.0	0.0	0.0	14.261812383069023	0.0
36	0.0	0.0	0.0	14.39396793109077	0.0
37	0.0	0.0	0.0	14.52894865709873	0.0
38	0.0	0.0	0.0	14.676956592709786	0.0
39	0.0	0.0	0.0	14.846624226215141	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	35	1.2107921E-7	45.000004	17
ACCCGGC	20	7.030918E-4	45.0	39
ATACGCC	20	7.030918E-4	45.0	6
CGTTAGG	25	3.8888425E-5	44.999996	2
GTTACGG	25	3.8888425E-5	44.999996	2
CGTTTTT	17740	0.0	44.429256	1
GATACCT	1625	0.0	43.338463	5
TGATACC	1640	0.0	42.80488	4
ACGGCTG	1465	0.0	42.696243	8
TACGGCT	1475	0.0	42.559322	7
CGGCTGT	1520	0.0	41.44737	9
CCGATGA	450	0.0	41.0	18
ATGATAC	5125	0.0	40.785366	3
AATCTGT	1925	0.0	40.675327	2
ATACCTG	1760	0.0	40.52557	6
GCGACTG	245	0.0	40.408165	11
GAATGAT	5300	0.0	40.33019	1
GAATCTG	1940	0.0	40.244843	1
AATGATA	5115	0.0	40.117302	2
ATACGGC	3445	0.0	40.036285	6
>>END_MODULE