##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933327.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	428309
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.956414644567356	31.0	28.0	31.0	19.0	33.0
2	29.347300663773115	31.0	30.0	33.0	16.0	34.0
3	29.498618987693465	31.0	30.0	33.0	16.0	34.0
4	32.439906702871056	35.0	32.0	37.0	19.0	37.0
5	33.78168098265505	35.0	33.0	37.0	30.0	37.0
6	33.19291679605145	35.0	33.0	37.0	26.0	37.0
7	32.11000235811061	35.0	31.0	35.0	25.0	37.0
8	33.28626062025314	35.0	33.0	36.0	28.0	37.0
9	33.02387528630031	35.0	31.0	37.0	25.0	39.0
10	31.522305158191866	35.0	27.0	39.0	15.0	39.0
11	32.651471250895966	35.0	30.0	39.0	17.0	39.0
12	33.36928012252836	35.0	31.0	39.0	25.0	39.0
13	33.70315356436591	35.0	32.0	39.0	25.0	39.0
14	33.776834014695	37.0	32.0	40.0	21.0	41.0
15	34.432377092239484	37.0	32.0	40.0	25.0	41.0
16	34.61441856229965	37.0	32.0	40.0	25.0	41.0
17	32.59514742860878	36.0	29.0	39.0	18.0	41.0
18	33.28945457601871	36.0	31.0	39.0	23.0	39.0
19	33.1001730059373	36.0	31.0	37.0	23.0	39.0
20	32.78894443030616	35.0	31.0	37.0	25.0	39.0
21	33.0360510752751	35.0	32.0	37.0	25.0	39.0
22	33.2351479889519	35.0	31.0	38.0	26.0	39.0
23	34.3058259340803	35.0	33.0	38.0	27.0	40.0
24	32.443060967665865	35.0	31.0	37.0	22.0	39.0
25	31.496260877077063	35.0	30.0	37.0	18.0	39.0
26	30.82292690557518	33.0	25.0	37.0	20.0	39.0
27	32.62758662554371	35.0	31.0	38.0	22.0	39.0
28	32.701750371811	35.0	31.0	38.0	22.0	39.0
29	32.88283692380968	35.0	31.0	38.0	22.0	40.0
30	31.69638975599392	35.0	30.0	38.0	18.0	40.0
31	32.10817423869216	35.0	30.0	38.0	21.0	40.0
32	31.95764506466126	35.0	30.0	38.0	18.0	40.0
33	31.795719912493084	35.0	30.0	38.0	15.0	40.0
34	30.797055396921383	35.0	29.0	38.0	10.0	40.0
35	30.232859921225096	35.0	26.0	38.0	8.0	40.0
36	29.543381063671323	35.0	24.0	38.0	7.0	40.0
37	29.692544401355097	35.0	24.0	38.0	7.0	40.0
38	29.481021879063945	35.0	23.0	38.0	7.0	40.0
39	29.19907590080993	34.0	23.0	38.0	7.0	40.0
40	28.99791038712705	34.0	22.0	38.0	7.0	40.0
41	28.735160830148327	34.0	21.0	38.0	7.0	40.0
42	29.125598574860675	35.0	22.0	38.0	7.0	40.0
43	29.09640002895106	35.0	21.0	38.0	7.0	40.0
44	29.015320714717646	35.0	21.0	38.0	7.0	40.0
45	29.22046466452958	35.0	22.0	38.0	7.0	40.0
46	28.989467884167738	35.0	20.0	38.0	7.0	40.0
47	29.0301371206302	35.0	20.0	38.0	7.0	40.0
48	28.722464389027547	34.0	20.0	38.0	7.0	40.0
49	28.450905771300626	34.0	20.0	38.0	7.0	40.0
50	28.65654235610272	34.0	20.0	38.0	7.0	40.0
51	27.288315211681287	33.0	19.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	20.0
11	13.0
12	25.0
13	36.0
14	102.0
15	180.0
16	407.0
17	733.0
18	1272.0
19	2140.0
20	3397.0
21	4973.0
22	6887.0
23	9666.0
24	13651.0
25	18445.0
26	23020.0
27	23778.0
28	22058.0
29	22333.0
30	24310.0
31	27819.0
32	32323.0
33	38166.0
34	43436.0
35	47637.0
36	36617.0
37	20379.0
38	4394.0
39	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.88683170327964	2.429087411191453	38.08161864448331	40.6024622410456
2	40.99049984940778	8.87583496961306	36.446350648713896	13.687314532265257
3	12.094072270253484	8.935371425769713	64.45159919590763	14.518957108069175
4	11.269900936006481	2.655092468288082	69.47904433481436	16.59596226089109
5	16.253686007064992	3.1362871198130327	64.23656752484771	16.37345934827426
6	14.08165600069109	8.247316773637724	65.87370333100635	11.797323894664833
7	55.66635303017215	0.9212974744868775	40.9090166211777	2.5033328741632794
8	57.744992517084626	5.417350557658139	34.39736265173041	2.4402942735268227
9	53.926020700008635	2.3884625352257367	34.263814208900584	9.421702555865041
10	28.097471685161878	19.596132698589102	40.51420820015456	11.792187416094455
11	18.63094167995536	17.162142285125924	50.542248703622846	13.664667331295863
12	15.542283725067648	15.935457812000214	52.675054691822965	15.847203771109175
13	14.922637628441148	16.17570492331471	56.35464115860278	12.547016289641357
14	14.372800945112058	17.84389307719427	51.67717699137771	16.106128986315955
15	12.410899607526343	16.87590034297668	56.119764002157325	14.593436047339653
16	13.882734194238273	16.806324405977925	50.750509562021804	18.56043183776199
17	14.503314196059385	16.828504654350013	52.71171047071157	15.956470678879034
18	14.376069613293208	16.864926956939964	52.0446686854584	16.71433474430843
19	14.541137356441261	18.91648319320864	49.74399323852639	16.798386211823708
20	16.19228174051911	18.115659488826992	52.55551482691234	13.136543943741552
21	16.572614631025733	20.10417712445921	49.60367398303561	13.719534261479446
22	15.500491467608665	16.411282508656132	51.15302270090052	16.93520332283468
23	14.938747493048243	20.317340985129896	50.175924391035444	14.56798713078642
24	15.119691624504739	17.573060570756162	50.15677933454585	17.150468470193246
25	14.145861982820815	20.895895253193373	48.534119058903734	16.424123705082078
26	14.455918507432719	18.8543784977668	50.4430212766951	16.246681718105386
27	15.263746500773973	19.177276218804646	48.793744703006475	16.765232577414903
28	13.229234034307009	19.53402800314726	51.334667261252974	15.90207070129276
29	16.470818964812786	18.50369709718918	48.975155787060274	16.050328150937755
30	15.886894741880278	20.053979720248698	49.522657707402836	14.536467830468188
31	16.69308840113096	20.05117800466486	46.872001288789164	16.383732305415016
32	18.14671183654791	20.899630873971827	46.24908652398152	14.70457076549874
33	16.162863726888766	19.772874256669834	45.781666974077126	18.282595042364274
34	18.44112544915003	23.182795598504818	42.62156527180143	15.754513680543718
35	17.961798608014306	22.33597706328842	41.05517278413482	18.647051544562455
36	17.07879124650661	24.791680772526377	40.703090525765276	17.426437455201736
37	20.278583919553405	23.460165441305225	40.15161950834561	16.10963113079576
38	17.65874637236201	24.098022689226703	41.17144398086428	17.071786957547005
39	21.68084256926658	23.084735553070328	38.70173169370712	16.532690183955975
40	18.50323014459187	22.925504717388616	40.13691050153044	18.43435463648908
41	18.717094434158515	24.091018400267096	40.242208312223184	16.9496788533512
42	17.73182445384057	23.600484696796002	40.337933594671135	18.32975725469229
43	18.635144253331124	23.177892596233093	39.828721787307764	18.358241363128023
44	20.105811458549784	23.372378353011495	37.75241706338181	18.76939312505691
45	17.504885491549324	23.805710363312468	38.11127013441231	20.5781340107259
46	19.883075069634305	24.42208779175782	36.9800774674359	18.714759671171983
47	17.144164610129604	22.876007742074066	41.45184901554719	18.527978632249148
48	19.561111253791072	23.640175667567107	36.76527927267463	20.03343380596719
49	17.513991067196812	22.673817267440096	39.256471379307925	20.555720286055163
50	17.873311090824615	22.073316227303184	39.52893821983661	20.524434462035586
51	18.413808722207563	22.27457279674254	37.762456544223916	21.549161936825982
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	353.5
2	650.0
3	18563.5
4	36477.0
5	25323.0
6	14169.0
7	13527.5
8	12886.0
9	12573.0
10	12260.0
11	11627.5
12	10995.0
13	10392.5
14	9790.0
15	9184.5
16	8579.0
17	7850.0
18	7121.0
19	6659.5
20	6198.0
21	5723.5
22	5249.0
23	5102.0
24	4955.0
25	4789.5
26	4892.5
27	5161.0
28	5599.5
29	6038.0
30	6828.5
31	7619.0
32	8359.5
33	9100.0
34	9842.5
35	10585.0
36	11818.0
37	13051.0
38	13709.0
39	14367.0
40	15020.0
41	15673.0
42	16484.0
43	17295.0
44	18623.5
45	19952.0
46	29955.5
47	39959.0
48	34099.5
49	28240.0
50	26065.0
51	23890.0
52	21241.5
53	18593.0
54	16940.0
55	15287.0
56	14046.0
57	12805.0
58	11390.5
59	9976.0
60	9025.5
61	8075.0
62	7058.5
63	6042.0
64	5110.0
65	4178.0
66	3577.5
67	2977.0
68	2472.5
69	1968.0
70	1632.5
71	1297.0
72	1088.0
73	879.0
74	733.0
75	464.0
76	341.0
77	266.0
78	191.0
79	146.0
80	101.0
81	66.5
82	32.0
83	25.0
84	18.0
85	13.0
86	8.0
87	9.0
88	10.0
89	6.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	428309.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.88350354533278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.51108248935694	42.04824612719155
2	8.40793987711225	8.892826366004211
3	4.073995149087233	6.4634141073126985
4	2.515325370636027	5.320768726227834
5	1.6114180654944559	4.260871648979467
6	1.066728253711956	3.3847396432249726
7	0.7748705400041508	2.8684508264638406
8	0.5456941329512034	2.3086574091673815
9	0.3912202680812054	1.8620188590670619
>10	1.032873258070793	7.9632263584152705
>50	0.036901391049034366	1.3903068342425038
>100	0.027001017840604862	2.775853973731173
>500	0.0018000678560403242	0.5652137506525666
>1k	0.002250084820050405	2.6806600788844253
>5k	4.5001696401008104E-4	1.4543247285211935
>10k+	4.5001696401008104E-4	5.7604205619138416
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24205	5.651293808908989	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	6111	1.4267736610717963	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	4082	0.9530502511037592	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	3931	0.9177953300070744	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCT	1167	0.27246684052868375	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	1045	0.2439827320929516	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGTGCTT	1039	0.24258187430103031	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTC	779	0.18187803665110938	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTA	574	0.1340153954271332	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGAGTGCTTCGTATGCCG	517	0.12070724640388131	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCT	505	0.1179055308200388	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	474	0.110667765561779	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCC	456	0.10646519218601525	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	443	0.10343000030351918	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3347629865354218E-4	0.0	0.0	0.060470361351267425	0.0
2	2.3347629865354218E-4	0.0	0.0	0.4214247190696436	0.0
3	2.3347629865354218E-4	0.0	0.0	0.5701491213119501	0.0
4	2.3347629865354218E-4	0.0	0.0	1.0641849692628453	0.0
5	2.3347629865354218E-4	0.0	0.0	2.3436350858842565	0.0
6	2.3347629865354218E-4	0.0	0.0	2.7690289020310104	0.0
7	2.3347629865354218E-4	0.0	0.0	3.169907706819142	0.0
8	2.3347629865354218E-4	0.0	0.0	3.635225970035652	0.0
9	2.3347629865354218E-4	0.0	0.0	3.851891975186139	0.0
10	2.3347629865354218E-4	0.0	0.0	5.894342635807326	0.0
11	2.3347629865354218E-4	0.0	0.0	6.376938145124198	0.0
12	2.3347629865354218E-4	0.0	0.0	7.823790767880198	0.0
13	2.3347629865354218E-4	0.0	0.0	8.02621471881282	0.0
14	2.3347629865354218E-4	0.0	0.0	8.164432687615717	0.0
15	2.3347629865354218E-4	0.0	0.0	8.453476345348802	0.0
16	2.3347629865354218E-4	0.0	0.0	8.645627339140667	0.0
17	2.3347629865354218E-4	0.0	0.0	8.844082192996177	0.0
18	2.3347629865354218E-4	0.0	0.0	9.031096708217666	0.0
19	2.3347629865354218E-4	0.0	0.0	9.48684244318938	0.0
20	2.3347629865354218E-4	0.0	0.0	9.670354813931064	0.0
21	2.3347629865354218E-4	0.0	0.0	9.871377907071764	0.0
22	2.3347629865354218E-4	0.0	0.0	10.13940869792603	0.0
23	2.3347629865354218E-4	0.0	0.0	10.371717615086304	0.0
24	2.3347629865354218E-4	0.0	0.0	10.567604229656627	0.0
25	2.3347629865354218E-4	0.0	0.0	10.724967254949114	0.0
26	2.3347629865354218E-4	0.0	0.0	10.889101092902553	0.0
27	2.3347629865354218E-4	0.0	0.0	11.043895878909852	0.0
28	4.6695259730708435E-4	0.0	0.0	11.202426285695607	0.0
29	4.6695259730708435E-4	0.0	0.0	11.360956692481363	0.0
30	4.6695259730708435E-4	0.0	0.0	11.53606391647152	0.0
31	4.6695259730708435E-4	0.0	0.0	11.702532517411495	0.0
32	4.6695259730708435E-4	0.0	0.0	11.876705836207037	0.0
33	4.6695259730708435E-4	0.0	0.0	12.056482586170265	0.0
34	4.6695259730708435E-4	0.0	0.0	12.228554618277926	0.0
35	4.6695259730708435E-4	0.0	0.0	12.421872993563058	0.0
36	4.6695259730708435E-4	0.0	0.0	12.595112407163986	0.0
37	4.6695259730708435E-4	0.0	0.0	12.783994732774703	0.0
38	4.6695259730708435E-4	0.0	0.0	12.988053017797899	0.0
39	4.6695259730708435E-4	0.0	0.0	13.186974824250717	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCG	20	7.0283253E-4	45.0	1
ACCGGTG	35	1.209537E-7	45.0	17
TCAAGCG	45	3.8380676E-10	45.0	17
CACCGCT	30	2.1623018E-6	44.999996	16
CGTTTTT	12785	0.0	44.56003	1
TACGGCT	525	0.0	42.428574	7
ACGGCTG	535	0.0	41.635517	8
AATGATC	200	0.0	41.625	2
CACCGGT	55	6.002665E-11	40.909092	16
GAATCTG	575	0.0	40.695652	1
ATACGGC	1175	0.0	40.595745	6
ATGATAC	2035	0.0	40.577393	3
GCGAGAC	50	1.0786607E-9	40.5	21
ACACGAC	50	1.0786607E-9	40.5	26
CACGACC	50	1.0786607E-9	40.5	27
ACGACCA	50	1.0786607E-9	40.5	28
TGCGTTG	50	1.0786607E-9	40.5	1
GTTTTTT	14245	0.0	40.293083	2
CGATGAA	95	0.0	40.263157	19
GAATGAT	2200	0.0	40.090908	1
>>END_MODULE