FastQCFastQC Report
Sat 14 Jan 2017
SRR2933326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825387
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258773.1351353970925153No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC178642.1643180714016577No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG159301.9300037436984103No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC118171.4316920426418154No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT43560.5277524361299609No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTC32280.391089270851128No Hit
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC26960.3266346574394799No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTGGAT26160.31694223437005914No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCT25880.31354988629576186No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTA22840.276718678631963No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTT15120.1831867960120525No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGATTGGATCGT10180.12333608355837929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG302.1647556E-644.99999614
CGTTTTT206000.044.180831
GATACCT19800.043.1818245
CCGATGA5400.042.91666418
TGATACC20200.042.6608924
TACGGCT13800.042.39137
ATACCTG20500.041.8170746
CGATGAA5650.041.41592819
ACGGCTG14150.041.342768
GAATCTG21400.040.584111
CACCCGT501.0804797E-940.516
ACACGAC1900.040.26315726
GAATGAT62800.039.8407631
ATGATAC60200.039.8048173
AATGATA60000.039.56252
AATCTGT22150.039.3115122
CGGCTGT14950.039.2809379
CGACCAC14900.039.26174512
TACCTGT22100.039.0950247
GTTTTTT237150.038.8804552
Produced by FastQC (version 0.11.5)