FastQCFastQC Report
Sat 14 Jan 2017
SRR2933325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594631
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377066.3410753896113725No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG73961.2437965730007348No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC57460.9663135625286942No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC48990.8238722838197133No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT20230.3402109879908717No Hit
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC15030.2527617968118043No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTGGAT13080.21996835011965404No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTC11960.20113313971185492No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTA8040.13520990328455798No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCT7310.1229333822151889No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTT6640.11166589027480908No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6210.10443451485038621No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCGATTGGATCGTATGCCG6050.1017437705064149No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG207.0305367E-445.026
TGATTCG207.0305367E-445.026
CGTTTTT159250.044.5196231
TACGGCT7250.041.2758647
GCGAGAC1100.040.90909221
ACCGGTG556.002665E-1140.90909217
GAATGAT28300.040.1501771
GTTTTTT179650.040.0027852
AATGATA26400.039.8863642
GATACCT10650.039.7183075
ATGATAC27100.039.354243
ATACGGC16700.039.3413166
TGGGCGA6700.039.2910466
ACGGCTG7700.039.1558468
CCGATGA1150.039.13043218
AGACACG1150.039.13043224
ACGACCA1150.039.13043228
TGATACG17000.039.0441174
ACGGCGA9750.039.08
GATACGG16950.038.8938065
Produced by FastQC (version 0.11.5)