##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933325.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594631
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.74695903846251	31.0	28.0	31.0	16.0	33.0
2	29.218094919370163	31.0	30.0	33.0	16.0	34.0
3	29.418824447430424	31.0	30.0	33.0	16.0	34.0
4	32.20314615282419	35.0	32.0	37.0	19.0	37.0
5	33.58186169237729	35.0	33.0	37.0	28.0	37.0
6	33.023631462200925	35.0	33.0	37.0	25.0	37.0
7	31.853909399274507	35.0	30.0	35.0	23.0	37.0
8	33.13185151800024	35.0	33.0	35.0	28.0	37.0
9	32.76685372945575	35.0	31.0	37.0	25.0	39.0
10	30.868441100447168	35.0	27.0	39.0	15.0	39.0
11	31.75389947715474	35.0	27.0	39.0	15.0	39.0
12	32.70353042475081	35.0	30.0	39.0	21.0	39.0
13	32.84985478389119	35.0	31.0	39.0	21.0	39.0
14	33.299172091599665	36.0	31.0	39.0	19.0	41.0
15	33.80786571840351	36.0	31.0	40.0	22.0	41.0
16	33.9330324184242	36.0	32.0	40.0	23.0	41.0
17	32.00440945729368	35.0	27.0	39.0	17.0	41.0
18	32.743669603502006	36.0	30.0	39.0	21.0	39.0
19	32.53787474921422	36.0	30.0	37.0	18.0	39.0
20	32.38683317889582	34.0	30.0	37.0	24.0	39.0
21	32.67941631028319	35.0	31.0	37.0	23.0	39.0
22	32.98331065820652	35.0	31.0	37.0	25.0	39.0
23	34.1149872778244	35.0	33.0	38.0	27.0	40.0
24	32.22430381194388	34.0	30.0	37.0	21.0	39.0
25	31.424917301654304	34.0	29.0	37.0	18.0	39.0
26	30.736665259631604	33.0	25.0	37.0	18.0	39.0
27	32.35132880727712	34.0	30.0	37.0	22.0	39.0
28	32.41443180728889	35.0	31.0	38.0	21.0	39.0
29	32.728009471420094	35.0	31.0	38.0	22.0	40.0
30	31.64022225548281	35.0	30.0	38.0	18.0	40.0
31	31.78743624197191	34.0	30.0	37.0	20.0	40.0
32	31.69253537067526	35.0	30.0	37.0	18.0	40.0
33	31.781760453121347	35.0	30.0	38.0	16.0	40.0
34	31.008406894359695	35.0	29.0	38.0	12.0	40.0
35	30.454999823419904	35.0	27.0	38.0	10.0	40.0
36	29.776057756827342	34.0	25.0	38.0	8.0	40.0
37	29.937182891574775	35.0	25.0	38.0	8.0	40.0
38	29.810292433458734	35.0	24.0	38.0	8.0	40.0
39	29.384006215619433	34.0	23.0	38.0	8.0	40.0
40	29.254278703935718	34.0	23.0	38.0	8.0	40.0
41	28.64869137330546	33.0	22.0	37.0	7.0	40.0
42	29.103319537662852	34.0	23.0	38.0	7.0	40.0
43	29.129497116699262	34.0	23.0	38.0	7.0	40.0
44	29.095590374534794	34.0	23.0	38.0	7.0	40.0
45	29.290586935427182	34.0	23.0	38.0	7.0	40.0
46	29.060363485926565	34.0	23.0	38.0	7.0	40.0
47	29.06240004305191	34.0	23.0	38.0	7.0	40.0
48	28.839100215091374	34.0	23.0	37.0	7.0	40.0
49	28.685228318066162	34.0	23.0	37.0	7.0	39.0
50	28.816773763897274	34.0	23.0	37.0	7.0	40.0
51	27.101916987173556	31.0	20.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	17.0
10	31.0
11	41.0
12	50.0
13	105.0
14	164.0
15	331.0
16	624.0
17	1091.0
18	1945.0
19	3104.0
20	4809.0
21	6922.0
22	9587.0
23	13333.0
24	17952.0
25	24864.0
26	30851.0
27	33450.0
28	33321.0
29	34619.0
30	38151.0
31	43259.0
32	48571.0
33	54098.0
34	59625.0
35	62732.0
36	43932.0
37	22460.0
38	4496.0
39	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.009047627856607	2.746913632151704	38.33822992746762	38.905808812524064
2	39.58959421893577	8.751141464202169	37.15615230285673	14.503112014005323
3	13.595994827044	8.664869473673589	62.62202946028713	15.117106238995277
4	12.59150632913521	3.112518519888805	66.60281754567117	17.6931576053048
5	16.958584399400635	3.5531279062141063	61.956574749718726	17.531712944666523
6	15.286118618100975	8.182049035452238	63.72372109762189	12.808111248824902
7	56.70171921746427	1.4292897612132567	38.82828174111339	3.040709280209071
8	58.17019294318661	5.276381487006227	33.023505333559804	3.5299202362473534
9	53.99213966308517	2.61977596189906	33.54618242237623	9.841901952639535
10	31.113076849340178	16.592474997099043	39.28268119220155	13.01176696135923
11	23.019149691153	16.144297892306323	46.20209844424525	14.634453972295425
12	19.531608678323195	14.528505913751555	49.93735610824192	16.00252929968333
13	17.591077491755392	14.88788845519322	53.70406184675874	13.816972206292641
14	15.491287874328785	15.954768587577842	51.25918426721782	17.294759270875552
15	13.591622367485046	15.109874863570852	54.97762477906466	16.32087798987944
16	16.130507827543468	15.20573263082483	48.81935183332184	19.84440770830986
17	17.02013517626898	15.583109525066806	49.965104409289125	17.431650889375092
18	16.74265216579694	15.189083650196508	50.24712805084162	17.821136133164938
19	16.81042528896072	18.01957852853282	47.03337027501089	18.136625907495574
20	17.926747848665812	18.710090795804458	49.35968693189558	14.003474423634152
21	18.315728577891164	19.04441578054289	47.387034984721616	15.25282065684433
22	16.896192764924802	15.7055383927175	48.94026715727905	18.458001685078646
23	17.023330435177446	18.672420374988857	48.347798887040874	15.956450302792824
24	17.23018140662024	16.881561842554458	47.491301328050504	18.396955422774795
25	16.746688282312896	19.36595972964746	45.61904777921097	18.268304208828667
26	16.052644413089798	16.72415329843214	47.79989606999971	19.423306218478352
27	16.772418525102122	16.77292303966662	47.208773171933515	19.245885263297744
28	14.554908842626771	17.752185809350674	48.253959178044866	19.438946169977683
29	17.947264774288595	16.459283152072462	45.90947999683838	19.683972076800572
30	18.395946393645808	17.88167788090429	47.07793572820791	16.644439997241985
31	18.565631458837498	18.463383173766587	45.265383069500245	17.70560229789567
32	19.80034676967733	18.549991507338166	45.25125666169439	16.398405061290113
33	18.895415812495482	18.42958069794545	43.64353691617154	19.03146657338753
34	20.012747401329563	19.95136479598272	41.779691943406924	18.256195859280798
35	20.46950125371869	22.201331582107223	39.61162468825204	17.717542475922045
36	19.57163350043977	24.22695755855312	37.73028987725161	18.471119063755506
37	20.45487033134835	22.855855143778243	40.05273858914184	16.636535935731573
38	20.025023922398933	23.00418242573966	39.636177730390784	17.334615921470625
39	21.56698860301599	21.437832874505368	38.85064855347265	18.144529969005987
40	22.82877952881703	20.733866885513873	39.44193962306035	16.995413962608744
41	20.3469042145465	24.30633451670027	36.70293005241906	18.643831216334164
42	19.71659735197122	22.18838237495186	39.41805926700761	18.67696100606931
43	19.726519471739618	21.822777487214758	38.16064080076551	20.290062240280108
44	20.96964335865436	21.014040640329885	37.3184378211025	20.697878179913257
45	19.2406719461313	21.399489767603775	36.93012977796314	22.429708508301786
46	20.430989975295606	22.224875595116973	36.99336227004647	20.35077215954096
47	17.53507637509649	21.168590268586737	41.4906050979515	19.80572825836527
48	19.413215927188457	22.451234463053556	36.96595031204225	21.169599297715727
49	18.837901152143093	21.118306983658776	39.4000649142073	20.643726949990835
50	18.5558775105906	20.669289021258564	38.82306842394695	21.95176504420388
51	18.789131410908613	20.666261933871592	37.50527638148701	23.039330273732787
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	142.0
1	604.0
2	1066.0
3	27645.5
4	54225.0
5	36087.5
6	17950.0
7	16937.5
8	15925.0
9	15536.0
10	15147.0
11	14343.5
12	13540.0
13	12483.0
14	11426.0
15	10437.5
16	9449.0
17	8639.0
18	7829.0
19	7161.5
20	6494.0
21	6142.5
22	5791.0
23	5517.5
24	5244.0
25	5230.5
26	5444.0
27	5671.0
28	6271.5
29	6872.0
30	7208.0
31	7544.0
32	8491.0
33	9438.0
34	10405.0
35	11372.0
36	12218.5
37	13065.0
38	13835.0
39	14605.0
40	15823.0
41	17041.0
42	18058.0
43	19075.0
44	20890.5
45	22706.0
46	37185.5
47	51665.0
48	44963.0
49	38261.0
50	36858.0
51	35455.0
52	32147.5
53	28840.0
54	27356.0
55	25872.0
56	24946.0
57	24020.0
58	22974.0
59	21928.0
60	20361.5
61	18795.0
62	17182.0
63	15569.0
64	13650.5
65	11732.0
66	10149.5
67	8567.0
68	7264.5
69	5962.0
70	5037.0
71	4112.0
72	3476.0
73	2840.0
74	2355.0
75	1511.0
76	1152.0
77	883.5
78	615.0
79	448.5
80	282.0
81	217.0
82	152.0
83	105.5
84	59.0
85	44.0
86	29.0
87	21.0
88	13.0
89	8.0
90	3.0
91	3.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	594631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.01325856649156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63700915818414	37.7561905360746
2	7.812748736922144	7.502310504417464
3	3.8101884725691084	5.488186929615784
4	2.5552232171708598	4.9073837208450755
5	1.8038548110581083	4.330447372987136
6	1.3549990914083105	3.9034753039889005
7	0.9999161274490554	3.3606462100411494
8	0.7335015709154746	2.8174240466633944
9	0.5622472562947767	2.4295790605303402
>10	1.6379319989880066	11.355215478572099
>50	0.05191874459871923	1.709708807168097
>100	0.03544797017421296	3.128535010483121
>500	0.002148361828740178	0.6904163425706237
>1k	0.0017903015239501485	1.8788745537734926
>5k	7.161206095800594E-4	2.259325591151179
>10k+	3.580603047900297E-4	6.482280531117514
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37706	6.3410753896113725	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	7396	1.2437965730007348	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC	5746	0.9663135625286942	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC	4899	0.8238722838197133	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT	2023	0.3402109879908717	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	1503	0.2527617968118043	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTGGAT	1308	0.21996835011965404	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTC	1196	0.20113313971185492	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTA	804	0.13520990328455798	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCT	731	0.1229333822151889	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTT	664	0.11166589027480908	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	621	0.10443451485038621	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCGATTGGATCGTATGCCG	605	0.1017437705064149	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09266250834551175	0.0
2	0.0	0.0	0.0	0.5028328492796373	0.0
3	0.0	0.0	0.0	0.655700762321507	0.0
4	0.0	0.0	0.0	1.3063563789980677	0.0
5	0.0	0.0	0.0	2.730600994566378	0.0
6	0.0	0.0	0.0	3.222502694948632	0.0
7	0.0	0.0	0.0	3.694896498837094	0.0
8	0.0	0.0	0.0	4.326212390541361	0.0
9	0.0	0.0	0.0	4.6726457248276665	0.0
10	0.0	0.0	0.0	6.6553879632915205	0.0
11	0.0	0.0	0.0	7.336482625359256	0.0
12	0.0	0.0	0.0	8.751309635723667	0.0
13	0.0	0.0	0.0	8.995999199503558	0.0
14	0.0	0.0	0.0	9.150044313195915	0.0
15	0.0	0.0	0.0	9.486387356192328	0.0
16	0.0	0.0	0.0	9.699292502409056	0.0
17	0.0	0.0	0.0	9.904461758636868	0.0
18	0.0	0.0	0.0	10.125270966364013	0.0
19	0.0	0.0	0.0	10.581856647231644	0.0
20	0.0	0.0	0.0	10.80350671256628	0.0
21	0.0	0.0	0.0	11.05946376828655	0.0
22	0.0	0.0	0.0	11.391098008681015	0.0
23	0.0	0.0	0.0	11.65680901264818	0.0
24	0.0	0.0	0.0	11.870891359515397	0.0
25	0.0	0.0	0.0	12.0582344344644	0.0
26	0.0	0.0	0.0	12.240868706811451	0.0
27	0.0	0.0	0.0	12.431743383711915	0.0
28	0.0	0.0	0.0	12.605128222376566	0.0
29	0.0	0.0	0.0	12.813829080555841	0.0
30	0.0	0.0	0.0	13.045233094137373	0.0
31	0.0	0.0	0.0	13.240143887553794	0.0
32	1.6817152149820646E-4	0.0	0.0	13.434045651841226	0.0
33	1.6817152149820646E-4	0.0	0.0	13.627611073085662	0.0
34	1.6817152149820646E-4	0.0	0.0	13.847411251683818	0.0
35	1.6817152149820646E-4	0.0	0.0	14.113290427172482	0.0
36	1.6817152149820646E-4	0.0	0.0	14.331745233598651	0.0
37	1.6817152149820646E-4	0.0	0.0	14.532373858746013	0.0
38	1.6817152149820646E-4	0.0	0.0	14.80363452292262	0.0
39	1.6817152149820646E-4	0.0	0.0	15.255006886623805	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	20	7.0305367E-4	45.0	26
TGATTCG	20	7.0305367E-4	45.0	26
CGTTTTT	15925	0.0	44.519623	1
TACGGCT	725	0.0	41.275864	7
GCGAGAC	110	0.0	40.909092	21
ACCGGTG	55	6.002665E-11	40.909092	17
GAATGAT	2830	0.0	40.150177	1
GTTTTTT	17965	0.0	40.002785	2
AATGATA	2640	0.0	39.886364	2
GATACCT	1065	0.0	39.718307	5
ATGATAC	2710	0.0	39.35424	3
ATACGGC	1670	0.0	39.341316	6
TGGGCGA	670	0.0	39.291046	6
ACGGCTG	770	0.0	39.155846	8
CCGATGA	115	0.0	39.130432	18
AGACACG	115	0.0	39.130432	24
ACGACCA	115	0.0	39.130432	28
TGATACG	1700	0.0	39.044117	4
ACGGCGA	975	0.0	39.0	8
GATACGG	1695	0.0	38.893806	5
>>END_MODULE