##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933325.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 594631 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.74695903846251 31.0 28.0 31.0 16.0 33.0 2 29.218094919370163 31.0 30.0 33.0 16.0 34.0 3 29.418824447430424 31.0 30.0 33.0 16.0 34.0 4 32.20314615282419 35.0 32.0 37.0 19.0 37.0 5 33.58186169237729 35.0 33.0 37.0 28.0 37.0 6 33.023631462200925 35.0 33.0 37.0 25.0 37.0 7 31.853909399274507 35.0 30.0 35.0 23.0 37.0 8 33.13185151800024 35.0 33.0 35.0 28.0 37.0 9 32.76685372945575 35.0 31.0 37.0 25.0 39.0 10 30.868441100447168 35.0 27.0 39.0 15.0 39.0 11 31.75389947715474 35.0 27.0 39.0 15.0 39.0 12 32.70353042475081 35.0 30.0 39.0 21.0 39.0 13 32.84985478389119 35.0 31.0 39.0 21.0 39.0 14 33.299172091599665 36.0 31.0 39.0 19.0 41.0 15 33.80786571840351 36.0 31.0 40.0 22.0 41.0 16 33.9330324184242 36.0 32.0 40.0 23.0 41.0 17 32.00440945729368 35.0 27.0 39.0 17.0 41.0 18 32.743669603502006 36.0 30.0 39.0 21.0 39.0 19 32.53787474921422 36.0 30.0 37.0 18.0 39.0 20 32.38683317889582 34.0 30.0 37.0 24.0 39.0 21 32.67941631028319 35.0 31.0 37.0 23.0 39.0 22 32.98331065820652 35.0 31.0 37.0 25.0 39.0 23 34.1149872778244 35.0 33.0 38.0 27.0 40.0 24 32.22430381194388 34.0 30.0 37.0 21.0 39.0 25 31.424917301654304 34.0 29.0 37.0 18.0 39.0 26 30.736665259631604 33.0 25.0 37.0 18.0 39.0 27 32.35132880727712 34.0 30.0 37.0 22.0 39.0 28 32.41443180728889 35.0 31.0 38.0 21.0 39.0 29 32.728009471420094 35.0 31.0 38.0 22.0 40.0 30 31.64022225548281 35.0 30.0 38.0 18.0 40.0 31 31.78743624197191 34.0 30.0 37.0 20.0 40.0 32 31.69253537067526 35.0 30.0 37.0 18.0 40.0 33 31.781760453121347 35.0 30.0 38.0 16.0 40.0 34 31.008406894359695 35.0 29.0 38.0 12.0 40.0 35 30.454999823419904 35.0 27.0 38.0 10.0 40.0 36 29.776057756827342 34.0 25.0 38.0 8.0 40.0 37 29.937182891574775 35.0 25.0 38.0 8.0 40.0 38 29.810292433458734 35.0 24.0 38.0 8.0 40.0 39 29.384006215619433 34.0 23.0 38.0 8.0 40.0 40 29.254278703935718 34.0 23.0 38.0 8.0 40.0 41 28.64869137330546 33.0 22.0 37.0 7.0 40.0 42 29.103319537662852 34.0 23.0 38.0 7.0 40.0 43 29.129497116699262 34.0 23.0 38.0 7.0 40.0 44 29.095590374534794 34.0 23.0 38.0 7.0 40.0 45 29.290586935427182 34.0 23.0 38.0 7.0 40.0 46 29.060363485926565 34.0 23.0 38.0 7.0 40.0 47 29.06240004305191 34.0 23.0 38.0 7.0 40.0 48 28.839100215091374 34.0 23.0 37.0 7.0 40.0 49 28.685228318066162 34.0 23.0 37.0 7.0 39.0 50 28.816773763897274 34.0 23.0 37.0 7.0 40.0 51 27.101916987173556 31.0 20.0 35.0 7.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 12.0 9 17.0 10 31.0 11 41.0 12 50.0 13 105.0 14 164.0 15 331.0 16 624.0 17 1091.0 18 1945.0 19 3104.0 20 4809.0 21 6922.0 22 9587.0 23 13333.0 24 17952.0 25 24864.0 26 30851.0 27 33450.0 28 33321.0 29 34619.0 30 38151.0 31 43259.0 32 48571.0 33 54098.0 34 59625.0 35 62732.0 36 43932.0 37 22460.0 38 4496.0 39 84.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.009047627856607 2.746913632151704 38.33822992746762 38.905808812524064 2 39.58959421893577 8.751141464202169 37.15615230285673 14.503112014005323 3 13.595994827044 8.664869473673589 62.62202946028713 15.117106238995277 4 12.59150632913521 3.112518519888805 66.60281754567117 17.6931576053048 5 16.958584399400635 3.5531279062141063 61.956574749718726 17.531712944666523 6 15.286118618100975 8.182049035452238 63.72372109762189 12.808111248824902 7 56.70171921746427 1.4292897612132567 38.82828174111339 3.040709280209071 8 58.17019294318661 5.276381487006227 33.023505333559804 3.5299202362473534 9 53.99213966308517 2.61977596189906 33.54618242237623 9.841901952639535 10 31.113076849340178 16.592474997099043 39.28268119220155 13.01176696135923 11 23.019149691153 16.144297892306323 46.20209844424525 14.634453972295425 12 19.531608678323195 14.528505913751555 49.93735610824192 16.00252929968333 13 17.591077491755392 14.88788845519322 53.70406184675874 13.816972206292641 14 15.491287874328785 15.954768587577842 51.25918426721782 17.294759270875552 15 13.591622367485046 15.109874863570852 54.97762477906466 16.32087798987944 16 16.130507827543468 15.20573263082483 48.81935183332184 19.84440770830986 17 17.02013517626898 15.583109525066806 49.965104409289125 17.431650889375092 18 16.74265216579694 15.189083650196508 50.24712805084162 17.821136133164938 19 16.81042528896072 18.01957852853282 47.03337027501089 18.136625907495574 20 17.926747848665812 18.710090795804458 49.35968693189558 14.003474423634152 21 18.315728577891164 19.04441578054289 47.387034984721616 15.25282065684433 22 16.896192764924802 15.7055383927175 48.94026715727905 18.458001685078646 23 17.023330435177446 18.672420374988857 48.347798887040874 15.956450302792824 24 17.23018140662024 16.881561842554458 47.491301328050504 18.396955422774795 25 16.746688282312896 19.36595972964746 45.61904777921097 18.268304208828667 26 16.052644413089798 16.72415329843214 47.79989606999971 19.423306218478352 27 16.772418525102122 16.77292303966662 47.208773171933515 19.245885263297744 28 14.554908842626771 17.752185809350674 48.253959178044866 19.438946169977683 29 17.947264774288595 16.459283152072462 45.90947999683838 19.683972076800572 30 18.395946393645808 17.88167788090429 47.07793572820791 16.644439997241985 31 18.565631458837498 18.463383173766587 45.265383069500245 17.70560229789567 32 19.80034676967733 18.549991507338166 45.25125666169439 16.398405061290113 33 18.895415812495482 18.42958069794545 43.64353691617154 19.03146657338753 34 20.012747401329563 19.95136479598272 41.779691943406924 18.256195859280798 35 20.46950125371869 22.201331582107223 39.61162468825204 17.717542475922045 36 19.57163350043977 24.22695755855312 37.73028987725161 18.471119063755506 37 20.45487033134835 22.855855143778243 40.05273858914184 16.636535935731573 38 20.025023922398933 23.00418242573966 39.636177730390784 17.334615921470625 39 21.56698860301599 21.437832874505368 38.85064855347265 18.144529969005987 40 22.82877952881703 20.733866885513873 39.44193962306035 16.995413962608744 41 20.3469042145465 24.30633451670027 36.70293005241906 18.643831216334164 42 19.71659735197122 22.18838237495186 39.41805926700761 18.67696100606931 43 19.726519471739618 21.822777487214758 38.16064080076551 20.290062240280108 44 20.96964335865436 21.014040640329885 37.3184378211025 20.697878179913257 45 19.2406719461313 21.399489767603775 36.93012977796314 22.429708508301786 46 20.430989975295606 22.224875595116973 36.99336227004647 20.35077215954096 47 17.53507637509649 21.168590268586737 41.4906050979515 19.80572825836527 48 19.413215927188457 22.451234463053556 36.96595031204225 21.169599297715727 49 18.837901152143093 21.118306983658776 39.4000649142073 20.643726949990835 50 18.5558775105906 20.669289021258564 38.82306842394695 21.95176504420388 51 18.789131410908613 20.666261933871592 37.50527638148701 23.039330273732787 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 142.0 1 604.0 2 1066.0 3 27645.5 4 54225.0 5 36087.5 6 17950.0 7 16937.5 8 15925.0 9 15536.0 10 15147.0 11 14343.5 12 13540.0 13 12483.0 14 11426.0 15 10437.5 16 9449.0 17 8639.0 18 7829.0 19 7161.5 20 6494.0 21 6142.5 22 5791.0 23 5517.5 24 5244.0 25 5230.5 26 5444.0 27 5671.0 28 6271.5 29 6872.0 30 7208.0 31 7544.0 32 8491.0 33 9438.0 34 10405.0 35 11372.0 36 12218.5 37 13065.0 38 13835.0 39 14605.0 40 15823.0 41 17041.0 42 18058.0 43 19075.0 44 20890.5 45 22706.0 46 37185.5 47 51665.0 48 44963.0 49 38261.0 50 36858.0 51 35455.0 52 32147.5 53 28840.0 54 27356.0 55 25872.0 56 24946.0 57 24020.0 58 22974.0 59 21928.0 60 20361.5 61 18795.0 62 17182.0 63 15569.0 64 13650.5 65 11732.0 66 10149.5 67 8567.0 68 7264.5 69 5962.0 70 5037.0 71 4112.0 72 3476.0 73 2840.0 74 2355.0 75 1511.0 76 1152.0 77 883.5 78 615.0 79 448.5 80 282.0 81 217.0 82 152.0 83 105.5 84 59.0 85 44.0 86 29.0 87 21.0 88 13.0 89 8.0 90 3.0 91 3.5 92 4.0 93 2.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 594631.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.01325856649156 #Duplication Level Percentage of deduplicated Percentage of total 1 78.63700915818414 37.7561905360746 2 7.812748736922144 7.502310504417464 3 3.8101884725691084 5.488186929615784 4 2.5552232171708598 4.9073837208450755 5 1.8038548110581083 4.330447372987136 6 1.3549990914083105 3.9034753039889005 7 0.9999161274490554 3.3606462100411494 8 0.7335015709154746 2.8174240466633944 9 0.5622472562947767 2.4295790605303402 >10 1.6379319989880066 11.355215478572099 >50 0.05191874459871923 1.709708807168097 >100 0.03544797017421296 3.128535010483121 >500 0.002148361828740178 0.6904163425706237 >1k 0.0017903015239501485 1.8788745537734926 >5k 7.161206095800594E-4 2.259325591151179 >10k+ 3.580603047900297E-4 6.482280531117514 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37706 6.3410753896113725 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG 7396 1.2437965730007348 No Hit GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC 5746 0.9663135625286942 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC 4899 0.8238722838197133 No Hit GAACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCT 2023 0.3402109879908717 No Hit GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC 1503 0.2527617968118043 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTGGAT 1308 0.21996835011965404 No Hit GAATGATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTC 1196 0.20113313971185492 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTA 804 0.13520990328455798 No Hit GAATGACTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCT 731 0.1229333822151889 No Hit GAATGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTT 664 0.11166589027480908 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 621 0.10443451485038621 No Hit GAATGATACCTGTCTCTTCTACACATCTGACGCCGATTGGATCGTATGCCG 605 0.1017437705064149 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.09266250834551175 0.0 2 0.0 0.0 0.0 0.5028328492796373 0.0 3 0.0 0.0 0.0 0.655700762321507 0.0 4 0.0 0.0 0.0 1.3063563789980677 0.0 5 0.0 0.0 0.0 2.730600994566378 0.0 6 0.0 0.0 0.0 3.222502694948632 0.0 7 0.0 0.0 0.0 3.694896498837094 0.0 8 0.0 0.0 0.0 4.326212390541361 0.0 9 0.0 0.0 0.0 4.6726457248276665 0.0 10 0.0 0.0 0.0 6.6553879632915205 0.0 11 0.0 0.0 0.0 7.336482625359256 0.0 12 0.0 0.0 0.0 8.751309635723667 0.0 13 0.0 0.0 0.0 8.995999199503558 0.0 14 0.0 0.0 0.0 9.150044313195915 0.0 15 0.0 0.0 0.0 9.486387356192328 0.0 16 0.0 0.0 0.0 9.699292502409056 0.0 17 0.0 0.0 0.0 9.904461758636868 0.0 18 0.0 0.0 0.0 10.125270966364013 0.0 19 0.0 0.0 0.0 10.581856647231644 0.0 20 0.0 0.0 0.0 10.80350671256628 0.0 21 0.0 0.0 0.0 11.05946376828655 0.0 22 0.0 0.0 0.0 11.391098008681015 0.0 23 0.0 0.0 0.0 11.65680901264818 0.0 24 0.0 0.0 0.0 11.870891359515397 0.0 25 0.0 0.0 0.0 12.0582344344644 0.0 26 0.0 0.0 0.0 12.240868706811451 0.0 27 0.0 0.0 0.0 12.431743383711915 0.0 28 0.0 0.0 0.0 12.605128222376566 0.0 29 0.0 0.0 0.0 12.813829080555841 0.0 30 0.0 0.0 0.0 13.045233094137373 0.0 31 0.0 0.0 0.0 13.240143887553794 0.0 32 1.6817152149820646E-4 0.0 0.0 13.434045651841226 0.0 33 1.6817152149820646E-4 0.0 0.0 13.627611073085662 0.0 34 1.6817152149820646E-4 0.0 0.0 13.847411251683818 0.0 35 1.6817152149820646E-4 0.0 0.0 14.113290427172482 0.0 36 1.6817152149820646E-4 0.0 0.0 14.331745233598651 0.0 37 1.6817152149820646E-4 0.0 0.0 14.532373858746013 0.0 38 1.6817152149820646E-4 0.0 0.0 14.80363452292262 0.0 39 1.6817152149820646E-4 0.0 0.0 15.255006886623805 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACG 20 7.0305367E-4 45.0 26 TGATTCG 20 7.0305367E-4 45.0 26 CGTTTTT 15925 0.0 44.519623 1 TACGGCT 725 0.0 41.275864 7 GCGAGAC 110 0.0 40.909092 21 ACCGGTG 55 6.002665E-11 40.909092 17 GAATGAT 2830 0.0 40.150177 1 GTTTTTT 17965 0.0 40.002785 2 AATGATA 2640 0.0 39.886364 2 GATACCT 1065 0.0 39.718307 5 ATGATAC 2710 0.0 39.35424 3 ATACGGC 1670 0.0 39.341316 6 TGGGCGA 670 0.0 39.291046 6 ACGGCTG 770 0.0 39.155846 8 CCGATGA 115 0.0 39.130432 18 AGACACG 115 0.0 39.130432 24 ACGACCA 115 0.0 39.130432 28 TGATACG 1700 0.0 39.044117 4 ACGGCGA 975 0.0 39.0 8 GATACGG 1695 0.0 38.893806 5 >>END_MODULE