##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933324.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	706400
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.576138165345412	31.0	28.0	33.0	25.0	34.0
2	28.990131653454135	31.0	28.0	33.0	16.0	34.0
3	29.005864949037374	31.0	28.0	34.0	16.0	34.0
4	33.24007361268403	35.0	32.0	37.0	28.0	37.0
5	34.2596050396376	35.0	33.0	37.0	30.0	37.0
6	33.790668176670444	35.0	33.0	37.0	28.0	37.0
7	33.502814269535676	35.0	32.0	37.0	28.0	37.0
8	33.65438561721405	35.0	33.0	37.0	28.0	37.0
9	34.68472253680634	37.0	34.0	39.0	28.0	39.0
10	34.443745753114385	37.0	32.0	39.0	27.0	39.0
11	34.715709229898074	37.0	34.0	39.0	27.0	39.0
12	34.86011325028313	37.0	34.0	39.0	27.0	39.0
13	35.14609711211778	37.0	34.0	39.0	29.0	39.0
14	35.87987117780295	38.0	34.0	40.0	27.0	41.0
15	36.24893544733862	38.0	34.0	40.0	30.0	41.0
16	36.04445356738392	38.0	34.0	40.0	29.0	41.0
17	35.43397791619479	37.0	33.0	40.0	27.0	41.0
18	35.15197904869762	37.0	33.0	39.0	27.0	40.0
19	34.45843006795017	37.0	33.0	38.0	27.0	40.0
20	34.18598952434881	35.0	33.0	38.0	27.0	40.0
21	34.42607446206115	35.0	33.0	38.0	27.0	40.0
22	34.99485843714609	35.0	33.0	39.0	30.0	40.0
23	35.37730464326161	36.0	34.0	39.0	30.0	40.0
24	34.810423272933186	35.0	33.0	39.0	28.0	40.0
25	33.68793742921857	35.0	33.0	39.0	24.0	40.0
26	33.823999150622875	35.0	33.0	38.0	25.0	40.0
27	34.51846687429219	35.0	33.0	39.0	27.0	40.0
28	34.361838901472254	35.0	33.0	39.0	25.0	40.0
29	34.43678369195923	35.0	33.0	39.0	25.0	40.0
30	33.6494238391846	35.0	33.0	39.0	22.0	40.0
31	33.455251981879954	35.0	33.0	39.0	22.0	40.0
32	33.031186296715745	35.0	32.0	39.0	19.0	40.0
33	32.70225226500566	36.0	32.0	39.0	15.0	40.0
34	32.22762457531144	36.0	31.0	40.0	12.0	41.0
35	31.827614665911664	36.0	30.0	40.0	10.0	41.0
36	31.187120611551528	35.0	29.0	40.0	8.0	41.0
37	31.12885758776897	36.0	28.0	40.0	8.0	41.0
38	30.73073046432616	35.0	26.0	39.0	8.0	41.0
39	30.487357021517553	35.0	25.0	39.0	7.0	40.0
40	30.37400906002265	35.0	24.0	39.0	7.0	40.0
41	29.941940826727066	35.0	23.0	39.0	7.0	40.0
42	30.092477349943376	35.0	23.0	39.0	7.0	40.0
43	29.95922140430351	35.0	23.0	39.0	7.0	40.0
44	30.037246602491507	35.0	23.0	39.0	7.0	40.0
45	30.048311155152888	35.0	23.0	39.0	7.0	40.0
46	29.858195073612684	35.0	23.0	39.0	7.0	40.0
47	29.785904586636466	35.0	23.0	39.0	7.0	40.0
48	29.682961494903736	35.0	23.0	38.0	7.0	40.0
49	29.84800537938845	35.0	24.0	39.0	7.0	40.0
50	29.819031710079276	35.0	24.0	39.0	7.0	40.0
51	28.609518686296717	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	41.0
10	37.0
11	42.0
12	55.0
13	74.0
14	105.0
15	191.0
16	369.0
17	753.0
18	1391.0
19	2312.0
20	3871.0
21	5696.0
22	8308.0
23	11734.0
24	17068.0
25	24490.0
26	32119.0
27	34326.0
28	32445.0
29	30133.0
30	30740.0
31	34199.0
32	40112.0
33	48124.0
34	56095.0
35	66467.0
36	75066.0
37	78893.0
38	61827.0
39	9304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.027463193657983	2.5669592298980746	35.08437146092865	37.32120611551529
2	36.25821064552661	13.117638731596829	35.59668742921857	15.027463193657983
3	13.759767836919593	13.35617214043035	57.99745186862967	14.886608154020383
4	12.022366930917327	3.07063986409966	66.87330124575311	18.0336919592299
5	19.495753114382786	4.022508493771235	58.23938278595696	18.242355605889017
6	14.781710079275198	12.403737259343147	60.9661664779162	11.84838618346546
7	57.39864099660249	1.4303510758776898	38.247451868629675	2.9235560588901475
8	57.07601925254813	8.326160815402037	31.654303510758773	2.9435164212910534
9	53.80124575311438	2.9963193657984144	31.12655719139298	12.075877689694224
10	31.454699886749715	18.661240090600227	36.90430351075878	12.97975651189128
11	23.737825594563986	17.319790486976217	44.308040770101925	14.63434314835787
12	17.77080973952435	16.10022650056625	48.20413363533409	17.924830124575312
13	17.35135900339751	16.54402604756512	52.59442242355606	13.510192525481312
14	16.07417893544734	20.492921857304644	46.65557757644394	16.777321630804074
15	13.875424688561722	15.923980747451868	53.92766138165346	16.272933182332956
16	16.300679501698752	17.70554926387316	45.64396942242356	20.349801812004532
17	16.246885617214044	17.740232163080407	48.70427519818799	17.308607021517552
18	16.619762174405437	17.128539071347678	47.72734994337486	18.524348810872027
19	16.518969422423556	18.863958097395244	45.191959229898075	19.425113250283125
20	19.03114382785957	18.934456398640997	48.79133635334088	13.243063420158553
21	19.167185730464325	21.83989241223103	44.94167610419026	14.051245753114383
22	17.766421291053227	15.850934314835788	47.544592298980746	18.838052095130237
23	17.19011891279728	21.346121177802946	46.65048131370328	14.813278595696488
24	17.625283125707814	18.444648924122312	45.685022650056624	18.24504530011325
25	15.896092865232164	24.38151189127973	43.128255945639864	16.594139297848244
26	16.60574745186863	17.55874858437146	46.406143827859566	19.42936013590034
27	18.330690826727068	18.884909399773502	44.45498301245753	18.3294167610419
28	15.120045300113249	19.30634201585504	47.619337485843715	17.954275198187997
29	20.42185730464326	17.81384484711212	43.84399773499434	17.920300113250285
30	18.80436013590034	20.053652321630803	45.77109286523216	15.370894677236693
31	19.009909399773502	18.240798414496037	44.359286523216305	18.390005662514156
32	20.45314269535674	23.048272933182332	41.0099093997735	15.48867497168743
33	20.08097395243488	19.352349943374858	38.86183465458664	21.704841449603624
34	20.891987542468858	21.251557191392976	39.74108154020385	18.115373725934315
35	20.049688561721403	23.03850509626274	39.01472253680634	17.897083805209512
36	20.8888731596829	25.115798414496037	36.60050962627406	17.394818799547
37	20.656002265005664	25.579133635334088	36.311438278595695	17.453425821064553
38	21.744337485843715	24.02477349943375	35.552095130237824	18.678793884484712
39	20.891138165345414	25.345554926387315	33.32686862967157	20.4364382785957
40	22.714184597961495	22.79742355605889	37.17780294450736	17.310588901472254
41	18.985560588901475	26.912938844847112	33.45144394110985	20.650056625141563
42	19.346545866364668	23.456257078142695	38.518120045300115	18.679077010192525
43	21.766987542468858	24.26174971687429	33.09258210645527	20.878680634201586
44	21.89864099660249	23.018686296715742	33.88250283125708	21.200169875424688
45	19.61169309173273	22.23272933182333	34.8728765571914	23.282701019252546
46	22.31823329558324	25.042893544733865	33.11452434881087	19.524348810872027
47	18.25552095130238	22.682049830124576	39.883069082672705	19.17936013590034
48	19.612400906002264	24.486834654586637	33.85461494903737	22.046149490373725
49	18.730181200453	22.52420724801812	38.15656851642129	20.589043035107586
50	19.263590033975085	22.753255945639864	36.29331823329559	21.689835787089468
51	19.133352208380522	21.92284824462061	34.572763306908264	24.3710362400906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	1033.5
2	1960.0
3	21246.5
4	40533.0
5	30440.5
6	20348.0
7	19438.0
8	18528.0
9	18060.0
10	17592.0
11	16932.5
12	16273.0
13	15309.0
14	14345.0
15	13122.5
16	11900.0
17	11135.0
18	10370.0
19	9593.5
20	8817.0
21	8266.0
22	7715.0
23	7695.5
24	7676.0
25	7535.5
26	7659.0
27	7923.0
28	8613.0
29	9303.0
30	10619.5
31	11936.0
32	12465.0
33	12994.0
34	14661.5
35	16329.0
36	17319.5
37	18310.0
38	19425.5
39	20541.0
40	21360.5
41	22180.0
42	23027.0
43	23874.0
44	26150.5
45	28427.0
46	56917.0
47	85407.0
48	62880.0
49	40353.0
50	38683.0
51	37013.0
52	34553.5
53	32094.0
54	31524.0
55	30954.0
56	29708.5
57	28463.0
58	26496.5
59	24530.0
60	22404.0
61	20278.0
62	18251.5
63	16225.0
64	14197.5
65	12170.0
66	10183.0
67	8196.0
68	6854.0
69	5512.0
70	4574.5
71	3637.0
72	3018.0
73	2399.0
74	2053.0
75	1359.5
76	1012.0
77	812.0
78	612.0
79	455.5
80	299.0
81	196.5
82	94.0
83	68.5
84	43.0
85	28.5
86	14.0
87	8.5
88	3.0
89	4.5
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	706400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.45551940283103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.47205802928966	35.40410598480372
2	5.712349146221096	4.964661983187122
3	2.4880275869031028	3.243555932323399
4	1.7150977197617399	2.9812184895543017
5	1.3991907861891517	3.0401281178752546
6	1.158451264217043	3.0204660833650707
7	0.9740602242929561	2.962980508340195
8	0.8261595312724653	2.8720953232835527
9	0.7252003022864744	2.8362560226253937
>10	3.420889189476447	22.30091746569844
>50	0.06537521691113783	1.9856113876184998
>100	0.03716763350780831	2.8723513391031013
>500	0.001991123223632555	0.549579824948379
>1k	0.002654830964843407	2.6514234220679342
>5k	0.0	0.0
>10k+	0.0013274154824217035	8.314648115205639
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18347	2.5972536806342017	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	14425	2.042044167610419	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	13130	1.8587202718006794	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	11755	1.664071347678369	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	3810	0.5393544733861835	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC	2754	0.38986409966024915	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGCTAT	2550	0.36098527746319364	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	2395	0.33904303510758776	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTA	2326	0.32927519818799544	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	2160	0.30577576443941107	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	1270	0.17978482446206118	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	1121	0.15869195922989807	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGT	983	0.13915628539071348	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.052095130237825596	0.0
2	0.0	0.0	0.0	0.45101925254813136	0.0
3	0.0	0.0	0.0	0.5223669309173273	0.0
4	0.0	0.0	0.0	1.1693091732729333	0.0
5	0.0	0.0	0.0	3.585928652321631	0.0
6	0.0	0.0	0.0	3.834088335220838	0.0
7	0.0	0.0	0.0	4.253822197055492	0.0
8	0.0	0.0	0.0	4.781993204983013	0.0
9	0.0	0.0	0.0	4.873442808607021	0.0
10	0.0	0.0	0.0	7.1620894677236695	0.0
11	0.0	0.0	0.0	7.348952434881087	0.0
12	0.0	0.0	0.0	9.45300113250283	0.0
13	0.0	0.0	0.0	9.64977349943375	0.0
14	0.0	0.0	0.0	9.776047565118914	0.0
15	0.0	0.0	0.0	10.22777463193658	0.0
16	0.0	0.0	0.0	10.462768969422424	0.0
17	0.0	0.0	0.0	10.57757644394111	0.0
18	0.0	0.0	0.0	10.686013590033975	0.0
19	0.0	0.0	0.0	11.230181200453002	0.0
20	0.0	0.0	0.0	11.366506228765571	0.0
21	0.0	0.0	0.0	11.475084937712344	0.0
22	0.0	0.0	0.0	11.683748584371461	0.0
23	0.0	0.0	0.0	11.809456398640997	0.0
24	0.0	0.0	0.0	11.92199886749717	0.0
25	0.0	0.0	0.0	12.023924122310305	0.0
26	0.0	0.0	0.0	12.147508493771234	0.0
27	0.0	0.0	0.0	12.287938844847112	0.0
28	0.0	0.0	0.0	12.383776896942242	0.0
29	0.0	0.0	0.0	12.501840317100793	0.0
30	0.0	0.0	0.0	12.6388731596829	0.0
31	0.0	0.0	0.0	12.753822197055493	0.0
32	0.0	0.0	0.0	12.891704416761042	0.0
33	0.0	0.0	0.0	13.015430351075878	0.0
34	0.0	0.0	0.0	13.190543601359003	0.0
35	0.0	0.0	0.0	13.348103057757644	0.0
36	0.0	0.0	0.0	13.494337485843715	0.0
37	0.0	0.0	0.0	13.646517553793885	0.0
38	1.4156285390713478E-4	0.0	0.0	13.830832389580975	0.0
39	1.4156285390713478E-4	0.0	0.0	14.066251415628539	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACG	25	3.8892726E-5	45.000004	26
TATACGG	25	3.8892726E-5	45.000004	27
ACGATAC	20	7.031438E-4	45.0	3
TACGCGC	30	2.16431E-6	44.999996	34
TCATACG	30	2.16431E-6	44.999996	31
CGTTTTT	16090	0.0	44.300808	1
TGATACC	1585	0.0	42.728706	4
GATACCT	1600	0.0	42.60938	5
ATCGACG	70	0.0	41.785717	1
ACGGCTG	1400	0.0	41.785713	8
CCGATGA	340	0.0	41.691177	18
CGATGTG	65	0.0	41.538464	10
AGCTACG	65	0.0	41.538464	9
TACGGCT	1410	0.0	41.48936	7
CTACGAA	60	3.6379788E-12	41.249996	11
CGATGAA	360	0.0	40.625004	19
GAATGAT	5315	0.0	40.216366	1
ATGATAC	5160	0.0	40.159885	3
ATACCTG	1710	0.0	40.13158	6
GACCGAT	225	0.0	40.0	9
>>END_MODULE