##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933323.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	484870
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.751479778084846	31.0	28.0	31.0	16.0	33.0
2	29.208777610493534	31.0	30.0	33.0	16.0	34.0
3	29.389135232124076	31.0	30.0	33.0	16.0	34.0
4	32.25012683812156	35.0	32.0	37.0	19.0	37.0
5	33.62968218285314	35.0	33.0	37.0	28.0	37.0
6	32.94697754037165	35.0	33.0	37.0	25.0	37.0
7	31.899245158496093	35.0	30.0	35.0	25.0	37.0
8	33.11716130096727	35.0	33.0	35.0	28.0	37.0
9	32.803596840390206	35.0	31.0	37.0	25.0	39.0
10	31.127819827995133	35.0	27.0	39.0	15.0	39.0
11	32.03993235300184	35.0	27.0	39.0	15.0	39.0
12	32.93080619547508	35.0	31.0	39.0	22.0	39.0
13	33.22407861901128	35.0	31.0	39.0	23.0	39.0
14	33.36995689566275	36.0	31.0	40.0	18.0	41.0
15	33.99299193598284	37.0	32.0	40.0	24.0	41.0
16	34.12821787283189	37.0	32.0	40.0	25.0	41.0
17	32.067482005486006	35.0	27.0	39.0	18.0	41.0
18	32.83855878895374	36.0	30.0	39.0	21.0	39.0
19	32.652789407470046	36.0	30.0	37.0	19.0	39.0
20	32.593901458122794	34.0	30.0	37.0	24.0	39.0
21	32.871718192505206	35.0	31.0	37.0	24.0	39.0
22	33.17694639800359	35.0	31.0	38.0	25.0	39.0
23	34.25655536535566	35.0	33.0	38.0	27.0	40.0
24	32.417934704147505	35.0	31.0	37.0	22.0	39.0
25	31.446736238579412	35.0	29.0	37.0	18.0	39.0
26	30.889306412027967	33.0	26.0	37.0	19.0	39.0
27	32.50477653804113	35.0	30.0	38.0	22.0	40.0
28	32.588434013240665	35.0	31.0	38.0	21.0	40.0
29	32.88309650009281	35.0	31.0	38.0	22.0	40.0
30	31.78545383298616	35.0	30.0	38.0	18.0	40.0
31	31.991152267618123	35.0	30.0	38.0	21.0	40.0
32	31.907100872398786	35.0	30.0	38.0	18.0	40.0
33	32.01385732258131	35.0	31.0	38.0	16.0	40.0
34	31.227326912368262	35.0	30.0	38.0	12.0	40.0
35	30.662410543032152	35.0	28.0	38.0	10.0	40.0
36	29.99116670447749	35.0	25.0	38.0	8.0	40.0
37	30.140697506548147	35.0	26.0	38.0	8.0	40.0
38	29.960333697692164	35.0	25.0	38.0	8.0	40.0
39	29.609146781611567	34.0	24.0	38.0	8.0	40.0
40	29.46765937261534	34.0	23.0	38.0	8.0	40.0
41	28.981485759069443	34.0	23.0	38.0	7.0	40.0
42	29.407711345309053	34.0	23.0	38.0	7.0	40.0
43	29.371179903891765	34.0	23.0	38.0	7.0	40.0
44	29.396056674985047	34.0	23.0	38.0	7.0	40.0
45	29.53739765297915	35.0	23.0	38.0	7.0	40.0
46	29.321327366098128	34.0	23.0	38.0	7.0	40.0
47	29.37331862148617	34.0	23.0	38.0	7.0	40.0
48	29.13478664384268	34.0	23.0	38.0	7.0	40.0
49	28.898999731886896	34.0	23.0	37.0	7.0	40.0
50	29.098915173139194	34.0	23.0	37.0	7.0	40.0
51	27.47810753397818	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	16.0
10	26.0
11	33.0
12	48.0
13	71.0
14	95.0
15	258.0
16	514.0
17	878.0
18	1540.0
19	2389.0
20	3761.0
21	5331.0
22	7482.0
23	10450.0
24	14183.0
25	19200.0
26	24238.0
27	26300.0
28	26106.0
29	26934.0
30	29907.0
31	34168.0
32	39504.0
33	44561.0
34	50088.0
35	53058.0
36	39180.0
37	20257.0
38	4222.0
39	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.311485552828593	2.560273887846227	37.54305277703302	38.58518778229217
2	38.86381916802442	9.058923010291418	37.43168271907934	14.645575102604822
3	14.064800874461195	9.134613401530308	62.22513250974488	14.575453214263618
4	12.588735124878832	3.0195722564811187	66.24373543424011	18.147957184399942
5	17.27040237589457	3.894239693113618	60.902097469424795	17.93326046156702
6	15.012477571307773	8.969208241384289	63.179202672881395	12.839111514426547
7	57.34609276713346	1.411306123290779	38.06154226906181	3.181058840513952
8	58.760492503145166	5.7578319962051685	32.18017200486729	3.301503495782375
9	54.62350733186215	2.9358384721677977	32.48293356982284	9.957720626147214
10	30.08455874770557	18.689751892259782	37.95388454637326	13.271804813661396
11	21.91845236867614	17.650916740569635	46.05564378080722	14.374987109946996
12	17.555220987068697	16.211355621094313	49.53698929609999	16.696434095737
13	16.543609627322788	16.595788561882564	53.149091509064284	13.711510301730362
14	15.628312743622002	17.775073731103184	49.21607853651494	17.380534988759873
15	13.85959123063914	15.819704250623879	53.978179718275	16.34252480046198
16	15.903231794089137	16.09338585600264	47.96089673520738	20.042485614700848
17	15.956648173737289	16.327262977705363	49.77973477426939	17.93635407428795
18	16.198774929362507	16.08389877699177	49.28620042485615	18.43112586878957
19	16.397797347742692	18.121145874151836	46.962897271433576	18.518159506671893
20	18.32388062779714	18.42060758553839	49.74549879349104	13.51001299317343
21	19.062016623012354	19.767566564233714	46.13257161713449	15.037845195619445
22	17.634004991028522	16.293439478623135	48.12238331924021	17.950172211108132
23	16.885144471714067	20.338234990822283	46.995070843731305	15.781549693732341
24	16.71623321715099	17.899436962484792	47.383216119784684	18.001113700579538
25	15.82609771691381	20.9672695774125	45.17045806092354	18.03617464475014
26	16.324788087528617	18.05803617464475	47.28525171695506	18.331924020871572
27	16.60919421700662	18.394002516138347	46.6706539897292	18.32614927712583
28	15.15251510714212	18.376059562356918	49.0102501701487	17.461175160352262
29	18.9143481757997	17.291026460700806	45.73927031987955	18.05535504361994
30	17.906242910470848	18.707282364345083	47.175531585785876	16.21094313939819
31	18.168375028358117	18.436900612535318	45.73679542970281	17.657928929403756
32	19.13481964237837	21.383257367954297	43.37327531090808	16.108647678759255
33	18.372347227091797	19.422525625425372	41.591973106193414	20.613154041289416
34	19.857487573988905	20.750716686947015	41.653226638067935	17.738569100996145
35	19.386020995318333	22.663600552725473	39.9841194546992	17.966258997256997
36	20.31534225668736	23.244993503413287	38.947140470641614	17.49252376925774
37	19.825107760843114	24.709922247200282	38.243034215356694	17.221935776599913
38	20.579949264751377	23.217150988924868	38.12650813620145	18.0763916101223
39	19.955451976818527	24.046651679831708	36.279208860106834	19.71868748324293
40	21.61795945304927	21.938045249242062	38.95889619898117	17.485099098727495
41	18.360797739600304	24.911625796605275	36.90514983397612	19.822426629818303
42	18.785860127456843	22.796419658877635	39.78241590529421	18.635304308371317
43	20.57788685627075	23.036071524326108	36.336750056716234	20.049291562686903
44	20.71606822447254	22.442097881906488	36.4629694557304	20.378864437890567
45	19.32291129581125	21.70334316414709	37.04023758945697	21.933507950584694
46	20.65068987563677	23.999216284777365	35.914987522428696	19.435106317157178
47	18.07907274114711	21.820075484150394	40.97139439437375	19.12945738032875
48	19.02592447460144	23.122898921360367	36.69725905912925	21.153917544908946
49	18.217666591045024	22.12923051539588	39.1511126693753	20.501990224183803
50	18.571988368016168	21.964031596097925	38.02586260234702	21.438117433538885
51	18.51465341225483	21.282199352403737	36.870088889805515	23.333058345535918
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	124.0
1	460.0
2	796.0
3	20650.0
4	40504.0
5	27384.0
6	14264.0
7	13469.5
8	12675.0
9	12571.5
10	12468.0
11	11856.0
12	11244.0
13	10382.5
14	9521.0
15	8763.0
16	8005.0
17	7276.5
18	6548.0
19	6020.5
20	5493.0
21	5144.5
22	4796.0
23	4785.5
24	4775.0
25	4743.0
26	4828.5
27	4946.0
28	5398.0
29	5850.0
30	6629.5
31	7409.0
32	7939.0
33	8469.0
34	9570.5
35	10672.0
36	11404.5
37	12137.0
38	12865.5
39	13594.0
40	14256.5
41	14919.0
42	15839.5
43	16760.0
44	18166.5
45	19573.0
46	31305.0
47	43037.0
48	37606.0
49	32175.0
50	30592.5
51	29010.0
52	26413.5
53	23817.0
54	22290.5
55	20764.0
56	19687.0
57	18610.0
58	17395.0
59	16180.0
60	14852.5
61	13525.0
62	12357.5
63	11190.0
64	9828.0
65	8466.0
66	7245.0
67	6024.0
68	5065.0
69	4106.0
70	3489.0
71	2872.0
72	2372.5
73	1873.0
74	1573.5
75	1061.0
76	848.0
77	647.0
78	446.0
79	355.5
80	265.0
81	172.5
82	80.0
83	56.5
84	33.0
85	21.5
86	10.0
87	6.5
88	3.0
89	4.5
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	484870.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.78460754238323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.389668942731	36.3482483546267
2	7.042524518173397	6.44878442344361
3	3.3160331100246148	4.554698236200765
4	2.1267787574286623	3.8949492295339514
5	1.6522885245218344	3.782469082100782
6	1.2910131809547805	3.5465119093235047
7	1.0086971800707851	3.2327963163054694
8	0.8423512347500883	3.0853376556699774
9	0.646673205485269	2.664691102918619
>10	2.5865466963915424	17.171496311988975
>50	0.06112018103436767	1.9218234754417598
>100	0.031249415867006115	2.822139071160708
>500	0.0018382009333533008	0.6486732838580825
>1k	0.002297751166691626	2.866534161298254
>5k	4.595502333383252E-4	1.3021858429444284
>10k+	4.595502333383252E-4	5.708661543184397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27132	5.5957266896281475	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	6189	1.276424608657991	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	4927	1.0161486584032833	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	4499	0.9278775754325902	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	1639	0.3380287499742199	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	1302	0.2685255841772022	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGCTAT	1257	0.2592447460143956	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC	917	0.18912285767319076	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTA	857	0.17674840678944873	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	663	0.1367376822653495	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	646	0.13323158784828923	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGGTAGCTATCGTATGCCG	497	0.10250170148699651	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08538371109782003	0.0
2	0.0	0.0	0.0	0.5135397116752944	0.0
3	0.0	0.0	0.0	0.6568771010786396	0.0
4	0.0	0.0	0.0	1.2461072039928227	0.0
5	0.0	0.0	0.0	2.533256336750057	0.0
6	0.0	0.0	0.0	2.8380803101862355	0.0
7	0.0	0.0	0.0	3.1629096458844637	0.0
8	0.0	0.0	0.0	3.6281889991131644	0.0
9	0.0	0.0	0.0	3.8055561284468	0.0
10	0.0	0.0	0.0	5.6699733949306	0.0
11	0.0	0.0	0.0	6.2686905768556525	0.0
12	0.0	0.0	0.0	7.913667581001093	0.0
13	0.0	0.0	0.0	8.159919153587559	0.0
14	0.0	0.0	0.0	8.286551034297853	0.0
15	0.0	0.0	0.0	8.678614886464413	0.0
16	0.0	0.0	0.0	8.914966898343886	0.0
17	0.0	0.0	0.0	9.160599748386165	0.0
18	0.0	0.0	0.0	9.40932621114938	0.0
19	0.0	0.0	0.0	9.908841545156434	0.0
20	0.0	0.0	0.0	10.143131148555282	0.0
21	0.0	0.0	0.0	10.41722523563017	0.0
22	0.0	0.0	0.0	10.791552374863365	0.0
23	0.0	0.0	0.0	11.06729638872275	0.0
24	0.0	0.0	0.0	11.288180336997545	0.0
25	0.0	0.0	0.0	11.487615237073856	0.0
26	0.0	0.0	0.0	11.670344628457112	0.0
27	0.0	0.0	0.0	11.855548910017118	0.0
28	0.0	0.0	0.0	12.020747829315074	0.0
29	0.0	0.0	0.0	12.218532802606884	0.0
30	0.0	0.0	0.0	12.449316311588673	0.0
31	0.0	0.0	0.0	12.634933074844804	0.0
32	0.0	0.0	0.0	12.8349866974653	0.0
33	0.0	0.0	0.0	13.025965722771051	0.0
34	0.0	0.0	0.0	13.26108028956215	0.0
35	0.0	0.0	0.0	13.481345515292759	0.0
36	0.0	0.0	0.0	13.677068080103945	0.0
37	0.0	0.0	0.0	13.87609049848413	0.0
38	0.0	0.0	0.0	14.102336708808547	0.0
39	0.0	0.0	0.0	14.422216264153278	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGCG	20	7.029247E-4	45.0	21
TATGCGT	20	7.029247E-4	45.0	11
GCTTACG	20	7.029247E-4	45.0	1
CTAGCGC	20	7.029247E-4	45.0	22
CGTTTTT	12685	0.0	44.414665	1
TAGGGAT	70	0.0	41.785713	5
TGATACC	765	0.0	41.764706	4
GATACCT	765	0.0	41.764706	5
ACGGCTG	675	0.0	41.333332	8
GAATGAT	2475	0.0	41.18182	1
AATGATA	2350	0.0	40.88298	2
TACGGCT	685	0.0	40.729927	7
ATGATAC	2385	0.0	40.566036	3
AGACACG	190	0.0	40.263157	24
ACACGAC	185	0.0	40.135136	26
AACACGT	180	0.0	40.0	41
GTTTTTT	14385	0.0	39.603752	2
ATCCGGG	40	3.4527147E-7	39.375	3
TATACGG	40	3.4527147E-7	39.375	27
CTTACGG	40	3.4527147E-7	39.375	2
>>END_MODULE