FastQCFastQC Report
Sat 14 Jan 2017
SRR2933322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833720
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC232362.787026819555726No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225532.7051048313582498No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG192482.308688768411457No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC162291.946576788370196No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT60740.7285419565321691No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC43140.5174399078827424No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCACCAT37270.44703257688432574No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC36410.4367173631435014No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT31180.37398647027779114No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCACCATCGTA29040.348318380271554No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT18410.22081754066113324No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA14540.1743990788274241No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTCACCATCGT13310.15964592429112892No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATG8410.10087319483759537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG406.8102963E-945.09
ATACCGG207.0321705E-445.028
CGTTTTT179400.044.297661
TACGGCT19250.042.0779237
GATACCT21500.042.0697635
ACGGCTG19550.041.6624078
ACCGGTC603.6379788E-1241.25000417
TCTACGG603.6379788E-1241.2500042
TGATACC22000.041.2159084
AGACACG1150.041.08695624
CGGCTGT20100.040.970159
CCGATGA5900.040.80508418
CGATGAA5900.040.80508419
GAATCTG27800.040.3866921
GAATGAT72800.040.2094761
TGATACG48900.039.892644
ATGATAC70300.039.879093
ATACCTG22750.039.8571436
GATACGG48050.039.7554635
ATACGGC47200.039.7086876
Produced by FastQC (version 0.11.5)