##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933322.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	833720
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.46228829822962	31.0	28.0	33.0	25.0	34.0
2	28.709803051384156	31.0	28.0	33.0	16.0	34.0
3	28.77503478386029	31.0	28.0	34.0	16.0	34.0
4	33.105722544739244	35.0	32.0	37.0	28.0	37.0
5	34.16281005613396	35.0	33.0	37.0	30.0	37.0
6	33.657364582833566	35.0	33.0	37.0	28.0	37.0
7	33.393795278990545	35.0	32.0	37.0	28.0	37.0
8	33.51302235762606	35.0	33.0	37.0	28.0	37.0
9	34.54876577268148	37.0	34.0	39.0	28.0	39.0
10	34.34426666026963	37.0	32.0	39.0	27.0	39.0
11	34.74006620927889	37.0	34.0	39.0	27.0	39.0
12	34.83287074797294	37.0	34.0	39.0	27.0	39.0
13	35.07143165571175	37.0	34.0	39.0	29.0	39.0
14	35.82989252986614	38.0	34.0	40.0	27.0	41.0
15	36.13686609413232	38.0	34.0	40.0	29.0	41.0
16	35.99129323993667	38.0	34.0	40.0	29.0	41.0
17	35.35901381758864	37.0	33.0	40.0	27.0	41.0
18	35.153789041884565	37.0	33.0	39.0	27.0	40.0
19	34.515827855874875	37.0	33.0	38.0	27.0	40.0
20	34.09984647123735	35.0	32.0	38.0	27.0	40.0
21	34.49795494890371	35.0	33.0	39.0	27.0	40.0
22	35.10163604087703	36.0	33.0	39.0	30.0	40.0
23	35.46490548385549	36.0	34.0	39.0	30.0	40.0
24	34.92399366693854	36.0	34.0	39.0	29.0	40.0
25	33.78674375089958	35.0	33.0	39.0	24.0	40.0
26	33.956487789665594	35.0	33.0	39.0	25.0	40.0
27	34.685347598714195	35.0	33.0	39.0	27.0	40.0
28	34.48874322314446	35.0	33.0	39.0	25.0	40.0
29	34.543208751139474	36.0	33.0	39.0	25.0	40.0
30	33.850279470325766	35.0	33.0	39.0	23.0	40.0
31	33.8258012282301	35.0	33.0	39.0	23.0	40.0
32	33.439444897567526	35.0	33.0	39.0	21.0	40.0
33	33.04996761502663	36.0	33.0	39.0	16.0	41.0
34	32.67705464664396	36.0	32.0	40.0	12.0	41.0
35	32.29263901549681	37.0	31.0	40.0	10.0	41.0
36	31.852470853523965	36.0	31.0	40.0	9.0	41.0
37	31.829217243199157	37.0	30.0	40.0	8.0	41.0
38	31.4716571510819	36.0	30.0	40.0	8.0	41.0
39	31.11291560715828	36.0	28.0	39.0	8.0	40.0
40	30.85242647411601	35.0	26.0	39.0	8.0	40.0
41	30.42448903708679	35.0	25.0	39.0	7.0	40.0
42	30.679734203329655	35.0	26.0	39.0	7.0	40.0
43	30.567874106414624	35.0	25.0	39.0	7.0	40.0
44	30.686856498584657	36.0	26.0	39.0	7.0	40.0
45	30.801824353499978	36.0	26.0	39.0	7.0	40.0
46	30.57992131650914	35.0	25.0	39.0	7.0	40.0
47	30.504475123542676	35.0	25.0	39.0	7.0	40.0
48	30.37787386652593	35.0	25.0	39.0	7.0	40.0
49	30.597986134433622	35.0	26.0	39.0	7.0	40.0
50	30.57256033200595	35.0	26.0	39.0	7.0	40.0
51	29.377106222712662	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	33.0
10	65.0
11	51.0
12	70.0
13	99.0
14	123.0
15	238.0
16	356.0
17	739.0
18	1465.0
19	2662.0
20	4285.0
21	6308.0
22	9201.0
23	13244.0
24	18957.0
25	27087.0
26	34605.0
27	37091.0
28	34956.0
29	32861.0
30	34899.0
31	39967.0
32	47223.0
33	57039.0
34	67937.0
35	81860.0
36	93284.0
37	98497.0
38	76903.0
39	11605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.064146236146428	2.6820755169601305	36.4477282540901	34.80604999280334
2	33.400901981480594	15.815141774216762	36.05263157894737	14.731324665355276
3	12.473972076956292	15.81538166290841	56.646835868157176	15.063810391978121
4	11.381399030849686	2.9148874922036176	67.78726670824737	17.916446768699323
5	20.688840378064576	3.9214604423547477	56.70141054550688	18.688288634073793
6	14.936549441059348	13.555150410209663	59.98452717938877	11.523772969342225
7	57.46629563882358	1.2031617329559086	38.28467591037759	3.045866717842921
8	56.8273521086216	9.453773449119609	30.41068944009979	3.3081850021589982
9	53.38746821474836	2.985054934510387	29.45749172384014	14.16998512690112
10	31.606654512306292	19.884493594971932	35.65285707431752	12.855994818404259
11	22.49040445233412	17.51451326584465	44.46097010986902	15.534112171952213
12	17.798541476754785	15.760207263829585	47.464256584944586	18.976994674471044
13	17.65220937485007	16.63891954133282	52.096627164995446	13.612243918821667
14	15.519838794799213	21.838986710166484	44.78362040013434	17.857554094899967
15	12.731972364822722	17.12565369668474	53.52504437940796	16.617329559084585
16	15.193710118505013	19.02641174495034	43.86196804682627	21.91791008971837
17	15.200546946216956	19.31451806361848	47.38797198100082	18.096963009163748
18	15.050376625245887	18.846375281869214	46.65775080362712	19.445497289257784
19	15.132778390826656	20.25488173487502	44.06203521565993	20.550304658638392
20	17.368181163939933	19.960538310224056	48.56318668137984	14.108093844456171
21	17.4465048217627	23.172527947032577	45.04234035407571	14.338626877129013
22	16.05503046586384	16.543443842057286	46.65954996881447	20.741975723264407
23	16.177253754258025	22.060403972556735	46.3452957827568	15.41704649042844
24	17.653048985270832	18.709998560667852	44.42738569303843	19.209566761022888
25	14.640526795566858	26.302715539989446	41.908914263781604	17.147843400662094
26	15.335964112651729	18.513889555246365	45.769442978457995	20.38070335364391
27	17.724056037998366	19.43314302163796	42.983375713668856	19.859425226694814
28	13.668497817012906	20.326728398023317	46.940339682387375	19.064434102576406
29	16.64383725951159	20.397855395096677	42.8551552079835	20.10315213740824
30	14.680468262726096	22.076716403588733	44.57719618097203	18.66561915271314
31	17.057405363911148	18.514489276975482	43.6297557933119	20.79834956580147
32	18.156935182075518	22.840162164755554	39.287530585808184	19.715372067360747
33	15.846687137168352	22.836204001343376	37.87866429976491	23.438444561723358
34	17.25423403540757	23.74214364534856	37.77143405459867	21.232188264645206
35	15.823058101041118	23.230820899102817	35.466343616561915	25.47977738329415
36	16.900758048265605	27.92628220505685	36.50865998176846	18.66429976490908
37	16.269730844887974	23.50381423019719	36.670584848630234	23.555870076284606
38	18.004605862879625	26.10252842680996	35.56385836971645	20.329007340593964
39	19.238833181403827	24.30732140286907	33.425490572374414	23.028354843352684
40	18.417934078587535	25.10339202609989	35.53363239456892	20.945041500743656
41	17.519550928369238	28.135105311135632	32.89221801084297	21.45312574965216
42	17.243918821666746	23.4024612579763	37.5657295015113	21.787890418845656
43	20.207263829583074	25.763925538550115	32.830086839706375	21.198723792160436
44	19.924914839514464	23.254809768267524	33.70951878328455	23.110756608933457
45	17.416158902269345	22.539341745430118	34.92323561867294	25.121263733627597
46	21.892002111020485	24.93798877320923	32.89353739864703	20.276471717123254
47	16.005133618001246	23.090725903180925	41.10204864942666	19.802091829391163
48	18.50789233795519	24.503190519598906	33.512210334404834	23.47670680804107
49	16.910473540277312	22.224847670680802	38.734587151561676	22.13009163748021
50	18.19363815189752	22.391930144412992	36.83334932591278	22.581082377776713
51	17.917286379120085	22.39384925394617	34.42210814182219	25.266756225111546
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	1117.5
2	2146.0
3	23992.0
4	45838.0
5	34272.5
6	22707.0
7	21351.5
8	19996.0
9	19532.5
10	19069.0
11	18284.5
12	17500.0
13	16548.5
14	15597.0
15	14523.5
16	13450.0
17	12393.0
18	11336.0
19	10461.0
20	9586.0
21	9113.0
22	8640.0
23	8462.0
24	8284.0
25	8447.0
26	9531.0
27	10452.0
28	11453.5
29	12455.0
30	14557.0
31	16659.0
32	17412.0
33	18165.0
34	19677.0
35	21189.0
36	22295.5
37	23402.0
38	25102.5
39	26803.0
40	28030.5
41	29258.0
42	29962.0
43	30666.0
44	33682.5
45	36699.0
46	78696.0
47	120693.0
48	86484.5
49	52276.0
50	48194.0
51	44112.0
52	39349.0
53	34586.0
54	32768.5
55	30951.0
56	29781.5
57	28612.0
58	26628.5
59	24645.0
60	21929.0
61	19213.0
62	17331.5
63	15450.0
64	13691.0
65	11932.0
66	10247.0
67	8562.0
68	6863.0
69	5164.0
70	4254.5
71	3345.0
72	2793.0
73	2241.0
74	1866.0
75	1235.0
76	979.0
77	728.5
78	478.0
79	341.0
80	204.0
81	155.0
82	106.0
83	74.5
84	43.0
85	32.5
86	22.0
87	16.5
88	11.0
89	8.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	833720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.56802670101697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.202991738884	35.37854112282158
2	6.110450387285013	5.324405312569459
3	2.614253807233293	3.416936390303261
4	1.8426467402804612	3.2112192952432403
5	1.4772253606699974	3.217989697854493
6	1.1612213852403468	3.0355274590766
7	0.9691882567272582	2.955793389318369
8	0.7991960204143497	2.7855514845407106
9	0.7029146167305725	2.7562142511227425
>10	3.020232556706011	19.446244658528443
>50	0.06366066207389899	1.8834719299009268
>100	0.0298757833315747	2.566033697472264
>500	0.0025129163549799977	0.6809830586144067
>1k	0.002233703426648887	2.716390085541211
>5k	2.7921292833111086E-4	0.7388872986823698
>10k+	0.0011168517133244434	9.885810868409855
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	23236	2.787026819555726	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22553	2.7051048313582498	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	19248	2.308688768411457	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	16229	1.946576788370196	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	6074	0.7285419565321691	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC	4314	0.5174399078827424	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCACCAT	3727	0.44703257688432574	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	3641	0.4367173631435014	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	3118	0.37398647027779114	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCACCATCGTA	2904	0.348318380271554	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	1841	0.22081754066113324	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	1454	0.1743990788274241	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTCACCATCGT	1331	0.15964592429112892	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATG	841	0.10087319483759537	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06632922324041644	0.0
2	0.0	0.0	0.0	0.5859281293479826	0.0
3	0.0	0.0	0.0	0.6984359257304611	0.0
4	0.0	0.0	0.0	1.5865038622079355	0.0
5	0.0	0.0	0.0	4.902245358153817	0.0
6	0.0	0.0	0.0	5.263277839082666	0.0
7	0.0	0.0	0.0	5.792592237201938	0.0
8	0.0	0.0	0.0	6.4966655471861054	0.0
9	0.0	0.0	0.0	6.626565273712997	0.0
10	0.0	0.0	0.0	9.435901741591902	0.0
11	0.0	0.0	0.0	9.643285515520798	0.0
12	0.0	0.0	0.0	12.080674567000912	0.0
13	1.1994434582353787E-4	0.0	0.0	12.295614834716691	0.0
14	1.1994434582353787E-4	0.0	0.0	12.43558988629276	0.0
15	1.1994434582353787E-4	0.0	0.0	12.918845655615794	0.0
16	1.1994434582353787E-4	0.0	0.0	13.170608837499401	0.0
17	1.1994434582353787E-4	0.0	0.0	13.301348174447057	0.0
18	1.1994434582353787E-4	0.0	0.0	13.407139087463417	0.0
19	1.1994434582353787E-4	0.0	0.0	14.062994770426522	0.0
20	1.1994434582353787E-4	0.0	0.0	14.211485870556062	0.0
21	1.1994434582353787E-4	0.0	0.0	14.340066209278895	0.0
22	1.1994434582353787E-4	0.0	0.0	14.586551839946265	0.0
23	1.1994434582353787E-4	0.0	0.0	14.726167058484863	0.0
24	1.1994434582353787E-4	0.0	0.0	14.850189512066402	0.0
25	1.1994434582353787E-4	0.0	0.0	14.965695917094468	0.0
26	1.1994434582353787E-4	0.0	0.0	15.095835532313007	0.0
27	1.1994434582353787E-4	0.0	0.0	15.267595835532314	0.0
28	1.1994434582353787E-4	0.0	0.0	15.374346303315262	0.0
29	1.1994434582353787E-4	0.0	0.0	15.501847142925682	0.0
30	1.1994434582353787E-4	0.0	0.0	15.66281245502087	0.0
31	1.1994434582353787E-4	0.0	0.0	15.792472292856115	0.0
32	1.1994434582353787E-4	0.0	0.0	15.936645396536008	0.0
33	1.1994434582353787E-4	0.0	0.0	16.06930384301684	0.0
34	1.1994434582353787E-4	0.0	0.0	16.252458859089383	0.0
35	1.1994434582353787E-4	0.0	0.0	16.424459051000337	0.0
36	1.1994434582353787E-4	0.0	0.0	16.5873434726287	0.0
37	1.1994434582353787E-4	0.0	0.0	16.75274672551936	0.0
38	1.1994434582353787E-4	0.0	0.0	16.9338626877129	0.0
39	1.1994434582353787E-4	0.0	0.0	17.193422252075038	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	40	6.8102963E-9	45.0	9
ATACCGG	20	7.0321705E-4	45.0	28
CGTTTTT	17940	0.0	44.29766	1
TACGGCT	1925	0.0	42.077923	7
GATACCT	2150	0.0	42.069763	5
ACGGCTG	1955	0.0	41.662407	8
ACCGGTC	60	3.6379788E-12	41.250004	17
TCTACGG	60	3.6379788E-12	41.250004	2
TGATACC	2200	0.0	41.215908	4
AGACACG	115	0.0	41.086956	24
CGGCTGT	2010	0.0	40.97015	9
CCGATGA	590	0.0	40.805084	18
CGATGAA	590	0.0	40.805084	19
GAATCTG	2780	0.0	40.386692	1
GAATGAT	7280	0.0	40.209476	1
TGATACG	4890	0.0	39.89264	4
ATGATAC	7030	0.0	39.87909	3
ATACCTG	2275	0.0	39.857143	6
GATACGG	4805	0.0	39.755463	5
ATACGGC	4720	0.0	39.708687	6
>>END_MODULE