##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933321.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563033
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.581028110252863	31.0	28.0	31.0	16.0	33.0
2	28.89599011070399	31.0	30.0	33.0	16.0	34.0
3	29.1207957615273	31.0	30.0	33.0	16.0	34.0
4	32.05746732429538	35.0	30.0	37.0	19.0	37.0
5	33.53568618535681	35.0	33.0	37.0	28.0	37.0
6	32.79795678050842	35.0	33.0	37.0	19.0	37.0
7	31.794624826608743	35.0	30.0	35.0	25.0	37.0
8	32.99284056174327	35.0	32.0	35.0	28.0	37.0
9	32.66295226034708	35.0	31.0	37.0	25.0	39.0
10	31.027151161654825	35.0	27.0	38.0	15.0	39.0
11	32.129866277820305	35.0	27.0	39.0	16.0	39.0
12	32.92031905767513	35.0	31.0	39.0	23.0	39.0
13	33.15839924125229	35.0	31.0	39.0	25.0	39.0
14	33.27449900805104	36.0	31.0	39.0	18.0	41.0
15	33.85517900371737	36.0	32.0	39.0	24.0	41.0
16	33.99368953507166	36.0	32.0	40.0	25.0	41.0
17	31.901130129139855	35.0	27.0	39.0	17.0	41.0
18	32.76332293133795	36.0	30.0	39.0	21.0	39.0
19	32.66611903742765	36.0	30.0	37.0	19.0	39.0
20	32.53905721334274	34.0	30.0	37.0	24.0	39.0
21	32.87784374983349	35.0	31.0	37.0	24.0	39.0
22	33.343166386339696	35.0	31.0	38.0	26.0	39.0
23	34.38715492697586	35.0	33.0	39.0	27.0	40.0
24	32.600378663417594	35.0	31.0	37.0	22.0	39.0
25	31.493560768196534	35.0	29.0	37.0	18.0	39.0
26	31.158313988700485	33.0	27.0	37.0	20.0	39.0
27	32.81562714796468	35.0	31.0	38.0	23.0	40.0
28	32.810286430813115	35.0	31.0	38.0	22.0	40.0
29	33.14031504370082	35.0	31.0	38.0	22.0	40.0
30	31.99341601646795	35.0	30.0	38.0	19.0	40.0
31	32.25361035676417	35.0	30.0	38.0	21.0	40.0
32	32.29211964485208	35.0	30.0	38.0	20.0	40.0
33	32.45325940042591	35.0	31.0	38.0	18.0	40.0
34	31.740041880316074	35.0	31.0	38.0	15.0	40.0
35	31.286260308010366	35.0	30.0	38.0	12.0	40.0
36	30.753049998845537	35.0	29.0	38.0	10.0	40.0
37	31.00531762791879	35.0	30.0	38.0	10.0	40.0
38	30.855520013924583	35.0	29.0	38.0	10.0	40.0
39	30.444442865693485	35.0	27.0	38.0	9.0	40.0
40	30.163970850731662	35.0	26.0	38.0	8.0	40.0
41	29.667506167489293	34.0	24.0	38.0	8.0	40.0
42	30.15071585502093	35.0	26.0	38.0	8.0	40.0
43	30.13662964693011	35.0	26.0	38.0	8.0	40.0
44	30.196897872771224	35.0	26.0	38.0	8.0	40.0
45	30.455220209117407	35.0	27.0	38.0	8.0	40.0
46	30.222312013683034	35.0	26.0	38.0	8.0	40.0
47	30.249496921139613	35.0	26.0	38.0	7.0	40.0
48	30.02946718931217	35.0	26.0	38.0	8.0	40.0
49	29.81893246044193	34.0	26.0	38.0	8.0	40.0
50	30.006335330255954	35.0	26.0	38.0	8.0	40.0
51	28.377890816346465	33.0	23.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	19.0
10	26.0
11	36.0
12	35.0
13	64.0
14	122.0
15	247.0
16	431.0
17	875.0
18	1562.0
19	2634.0
20	3893.0
21	5671.0
22	7903.0
23	10983.0
24	14648.0
25	19780.0
26	25162.0
27	27143.0
28	27988.0
29	29805.0
30	34167.0
31	40239.0
32	47843.0
33	55058.0
34	62430.0
35	66511.0
36	47435.0
37	25266.0
38	4964.0
39	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.717554743682875	2.7147609465164564	39.20516204201175	36.36252226778892
2	35.93252970962625	10.927778655957999	38.34997238172541	14.78971925269034
3	13.27186861160891	10.721929265247331	60.902114085675265	15.104088037468497
4	12.105684746719996	2.9868586743583414	66.50729175732151	18.400164821600153
5	17.89451062371122	3.949679681297544	59.95030486667744	18.205504828313792
6	15.413661366207664	9.642418828026065	62.224416686055704	12.719503119710568
7	57.930885045814364	1.241667895132257	37.481284400736726	3.346162658316653
8	59.421028607559414	6.488429630234817	30.43462816566702	3.6559135965387464
9	55.014182117211604	2.8513426388861753	30.665165274504336	11.469309969397887
10	30.24121143876114	20.25067802420107	36.3630550962377	13.145055440800096
11	20.91866728948392	17.920796827184198	45.947040404381276	15.213495478950612
12	17.744075391673313	16.16743601174354	48.55221629993269	17.536272296650463
13	16.83347157271421	17.076618954839237	52.03034280406299	14.059566668383559
14	15.20052998669705	18.905463800523236	47.27325041338607	18.620755799393642
15	12.648281717057436	17.500928009548286	53.019094795509325	16.831695477884956
16	14.889535782094477	17.432370749139036	46.05147478034147	21.626618688425012
17	15.041747108961642	18.247775885250064	48.12027003745784	18.590206968330452
18	14.833766404455867	17.858633508160267	48.089543596911724	19.218056490472136
19	15.227704237584652	20.04127644383189	45.353469512444214	19.37754980613925
20	16.96135040042058	19.70683778748315	48.72680642164847	14.605005390447806
21	17.274120699852407	21.56818516854252	45.61899568941785	15.538698442187226
22	15.966382077071858	17.587068608767158	46.78358106896043	19.66296824520055
23	15.883971276994421	21.52857825385013	46.076517717433966	16.51093275172148
24	16.29407157306943	18.477957775121528	46.230149920164536	18.997820731644506
25	14.749579509549173	22.709858924787714	43.64113648755934	18.89942507810377
26	15.377429031690859	19.411473217378024	46.004941095814985	19.20615665511613
27	15.805467885541344	19.380036338900204	44.876765660272135	19.937730115286314
28	13.93204305964304	19.64982514346406	47.56488518434976	18.85324661254314
29	15.888589123550483	19.27826610518389	44.38194564084166	20.451199130423973
30	14.550124060223823	20.94282217916179	45.77173984473379	18.735313915880596
31	16.169389716055722	19.44521901913387	44.26934122866688	20.11605003614353
32	16.901673614157605	22.43918207280923	41.32137903106923	19.337765281963932
33	15.154351521136416	22.01096560947582	40.31770784305716	22.516975026330606
34	16.23226347301135	23.43113103494822	39.45399292759039	20.88261256445004
35	15.120428109897643	23.617443382537083	37.39905121014221	23.863077297423064
36	16.264588398903797	25.86296007516433	38.15424673154149	19.71820479439038
37	15.591448458616103	23.872490600018118	37.9753939822355	22.56066695913028
38	17.087808352263544	24.94738319068332	38.13666339273186	19.828145064321273
39	17.705178914912626	24.246180952093393	35.87072160956818	22.177918523425802
40	17.988288430695892	24.29182658920525	37.123046073675965	20.596838906422892
41	16.793687048538896	26.328296920429175	35.888127338894876	20.98988869213705
42	16.700619679485925	23.386195835768063	38.587436260396814	21.325748224349194
43	18.6406480614813	24.82501025694764	35.77179312757867	20.762548553992392
44	18.76834927970474	22.9931460500539	36.19841110556575	22.040093564675605
45	17.165601305784918	22.423019609863008	36.86959734154126	23.541781742810812
46	19.478076773475088	24.19094440290356	35.81299852761738	20.517980296003966
47	15.769945988956241	22.71785135151936	41.34304738798614	20.16915527153826
48	17.46984635003632	23.39187933922168	36.445110677349284	22.693163633392714
49	16.338651553283732	21.908307328344875	39.66019753726691	22.092843581104482
50	17.355110624066437	21.97295718012976	38.269160066994296	22.402772128809502
51	17.013745197883605	21.807602751526108	36.82572779925866	24.352924251331626
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	115.0
1	531.0
2	947.0
3	24578.0
4	48209.0
5	31539.0
6	14869.0
7	13843.5
8	12818.0
9	12598.5
10	12379.0
11	11779.0
12	11179.0
13	10679.0
14	10179.0
15	9288.0
16	8397.0
17	7811.0
18	7225.0
19	6609.0
20	5993.0
21	5646.5
22	5300.0
23	5230.0
24	5160.0
25	5189.5
26	5755.5
27	6292.0
28	7018.5
29	7745.0
30	8904.0
31	10063.0
32	10854.5
33	11646.0
34	12571.5
35	13497.0
36	14599.0
37	15701.0
38	16799.0
39	17897.0
40	18835.5
41	19774.0
42	20748.0
43	21722.0
44	23688.5
45	25655.0
46	42542.5
47	59430.0
48	49937.0
49	40444.0
50	37392.5
51	34341.0
52	30071.5
53	25802.0
54	23480.5
55	21159.0
56	19780.5
57	18402.0
58	17140.0
59	15878.0
60	14498.0
61	13118.0
62	11845.0
63	10572.0
64	9497.0
65	8422.0
66	7306.0
67	6190.0
68	5171.0
69	4152.0
70	3374.5
71	2597.0
72	2152.5
73	1708.0
74	1437.0
75	1008.0
76	850.0
77	649.5
78	449.0
79	319.0
80	189.0
81	141.5
82	94.0
83	73.0
84	52.0
85	36.0
86	20.0
87	12.0
88	4.0
89	6.5
90	9.0
91	5.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	563033.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.53700922312507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.24084834131037	36.08391241766505
2	7.478156709754755	6.810657821281538
3	3.4001938506144205	4.64503976207526
4	2.2365664441508906	4.073861871817246
5	1.6088633651353106	3.6631412948457336
6	1.2346859584173968	3.3734343525669606
7	0.9416695636456159	3.00165709234066
8	0.7578284247169643	2.76073919727062
9	0.6289268687139272	2.5775503759167546
>10	2.391952501301475	15.794296935690443
>50	0.040153986135300725	1.2517026442613723
>100	0.03339539438314316	3.005607070779351
>500	0.0035780779696224815	1.1195442793458323
>1k	0.0015902568753877696	1.3882059401720315
>5k	0.001192692656540827	4.1059612314947405
>10k+	3.975642188469424E-4	6.3446877124763965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35046	6.224501938607506	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	8783	1.559944088534775	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	7198	1.278433058097838	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	6699	1.1898059261180074	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	2719	0.48292018407446813	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	1820	0.32324925892443246	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCACCAT	1639	0.2911019425149148	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC	1490	0.2646381295590134	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCACCATCGTA	950	0.16872900877923674	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	807	0.14333085272088847	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	746	0.13249667426243222	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCACTCACCATCGTATGCCG	731	0.12983253201854955	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	707	0.12556990442833724	No Hit
GAATCTTTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	685	0.12166249580397596	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11136114579429625	0.0
2	0.0	0.0	0.0	0.6163049057515279	0.0
3	0.0	0.0	0.0	0.7978217973014016	0.0
4	0.0	0.0	0.0	1.6432429360268404	0.0
5	0.0	0.0	0.0	3.4468672351354184	0.0
6	0.0	0.0	0.0	3.962822783034032	0.0
7	0.0	0.0	0.0	4.448762328318233	0.0
8	0.0	0.0	0.0	5.113554622908426	0.0
9	0.0	0.0	0.0	5.390270197306375	0.0
10	0.0	0.0	0.0	7.579839902812091	0.0
11	0.0	0.0	0.0	8.206623768056224	0.0
12	0.0	0.0	0.0	10.082002298266708	0.0
13	0.0	0.0	0.0	10.352146321796413	0.0
14	0.0	0.0	0.0	10.502226334868471	0.0
15	0.0	0.0	0.0	10.881955409363217	0.0
16	0.0	0.0	0.0	11.111426861302979	0.0
17	0.0	0.0	0.0	11.368072564130344	0.0
18	0.0	0.0	0.0	11.619567591952869	0.0
19	0.0	0.0	0.0	12.196087973529083	0.0
20	0.0	0.0	0.0	12.447227782385758	0.0
21	0.0	0.0	0.0	12.740283429212852	0.0
22	0.0	0.0	0.0	13.130491463200203	0.0
23	0.0	0.0	0.0	13.437045430729638	0.0
24	0.0	0.0	0.0	13.681258469752217	0.0
25	0.0	0.0	0.0	13.913926892384637	0.0
26	0.0	0.0	0.0	14.131853727934242	0.0
27	0.0	0.0	0.0	14.335394195366879	0.0
28	0.0	0.0	0.0	14.53715856797026	0.0
29	0.0	0.0	0.0	14.756506279383268	0.0
30	0.0	0.0	0.0	15.02558464601542	0.0
31	0.0	0.0	0.0	15.246886061740609	0.0
32	0.0	0.0	0.0	15.479376874890104	0.0
33	0.0	0.0	0.0	15.690021721639761	0.0
34	0.0	0.0	0.0	15.925531895998992	0.0
35	0.0	0.0	0.0	16.190525244523855	0.0
36	0.0	0.0	0.0	16.415378849907555	0.0
37	0.0	0.0	0.0	16.635081780286413	0.0
38	0.0	0.0	0.0	16.86437562274325	0.0
39	0.0	0.0	0.0	17.213200647208957	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACTCG	20	7.030217E-4	45.0	44
TATTGCG	20	7.030217E-4	45.0	1
CGTTTTT	14045	0.0	44.359203	1
TACGGCT	1020	0.0	42.132355	7
GATACCT	1260	0.0	41.785713	5
TGATACC	1270	0.0	41.279526	4
GAATGAT	3470	0.0	41.239193	1
ATGATAC	3315	0.0	41.199093	3
AATGATA	3300	0.0	41.045452	2
ACGGCTG	1060	0.0	40.542454	8
TATACGG	50	1.0786607E-9	40.5	27
GTTTTTT	15595	0.0	40.35428	2
CACCGGT	145	0.0	40.34483	16
CCTGCAT	230	0.0	40.1087	13
GAATCTG	1010	0.0	40.09901	1
TGATACG	2145	0.0	39.96504	4
ATACGGC	2130	0.0	39.929577	6
CGGCTGT	1015	0.0	39.901478	9
GCATTAG	220	0.0	39.886364	16
GATACGG	2150	0.0	39.767437	5
>>END_MODULE