##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933320.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374653
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.317838106194266	31.0	28.0	31.0	25.0	34.0
2	28.438653901076464	31.0	27.0	33.0	16.0	34.0
3	28.488153037610804	31.0	26.0	34.0	16.0	34.0
4	32.98323515359546	35.0	32.0	37.0	28.0	37.0
5	34.1568304537799	35.0	33.0	37.0	30.0	37.0
6	33.45371850752563	35.0	33.0	37.0	28.0	37.0
7	33.40552724788004	35.0	32.0	37.0	28.0	37.0
8	33.36268760693228	35.0	33.0	37.0	28.0	37.0
9	34.530536256215754	37.0	34.0	39.0	28.0	39.0
10	34.33594819739866	37.0	32.0	39.0	27.0	39.0
11	34.67602554897465	37.0	33.0	39.0	27.0	39.0
12	34.7648570810857	37.0	33.0	39.0	27.0	39.0
13	34.99693583128922	37.0	34.0	39.0	27.0	39.0
14	35.66876015940083	38.0	34.0	40.0	27.0	41.0
15	36.01819550357264	38.0	34.0	40.0	27.0	41.0
16	35.99599896437503	38.0	34.0	40.0	30.0	41.0
17	35.25273786677272	37.0	33.0	40.0	27.0	41.0
18	35.07854201087406	37.0	33.0	39.0	27.0	40.0
19	34.51229003904947	37.0	33.0	38.0	27.0	40.0
20	33.958452221121945	35.0	32.0	38.0	27.0	40.0
21	34.35860649721209	35.0	33.0	38.0	27.0	40.0
22	34.98951830093446	36.0	33.0	39.0	29.0	40.0
23	35.262162587781226	36.0	34.0	39.0	30.0	40.0
24	34.795458731145885	36.0	33.0	39.0	27.0	40.0
25	33.64501285189228	35.0	32.0	39.0	24.0	40.0
26	33.81693994175944	35.0	32.0	38.0	25.0	40.0
27	34.50993319151321	35.0	33.0	39.0	27.0	40.0
28	34.28733521418486	35.0	33.0	39.0	25.0	40.0
29	34.35695697085036	36.0	33.0	39.0	25.0	40.0
30	33.61628760479697	35.0	33.0	39.0	23.0	40.0
31	33.73232831446699	35.0	33.0	39.0	23.0	40.0
32	33.31512092522948	35.0	33.0	39.0	21.0	40.0
33	32.99349798346737	36.0	32.0	39.0	16.0	40.0
34	32.5495378390137	36.0	31.0	40.0	13.0	41.0
35	32.17301075928926	36.0	31.0	40.0	10.0	41.0
36	31.65814767264642	36.0	30.0	40.0	8.0	41.0
37	31.570744128566968	36.0	30.0	40.0	8.0	41.0
38	31.42814284150934	36.0	29.0	40.0	8.0	41.0
39	30.998238369904954	35.0	28.0	39.0	8.0	40.0
40	30.96226107891836	35.0	27.0	39.0	8.0	40.0
41	30.40320776825489	35.0	24.0	39.0	8.0	40.0
42	30.727833488588107	35.0	26.0	39.0	7.0	40.0
43	30.649456964177517	35.0	26.0	39.0	7.0	40.0
44	30.72048802491906	36.0	26.0	39.0	7.0	40.0
45	30.853640568739607	36.0	26.0	39.0	7.0	40.0
46	30.568253824205332	35.0	25.0	39.0	7.0	40.0
47	30.513339009696974	35.0	25.0	39.0	7.0	40.0
48	30.369880929820393	35.0	25.0	39.0	7.0	40.0
49	30.581244511588057	35.0	26.0	39.0	7.0	40.0
50	30.447592839240578	35.0	25.0	39.0	7.0	40.0
51	29.286035344705635	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	18.0
10	16.0
11	21.0
12	47.0
13	40.0
14	64.0
15	113.0
16	175.0
17	347.0
18	687.0
19	1295.0
20	2105.0
21	3111.0
22	4358.0
23	6137.0
24	8427.0
25	12247.0
26	15852.0
27	16748.0
28	15810.0
29	15210.0
30	16031.0
31	18468.0
32	22126.0
33	26053.0
34	30538.0
35	36208.0
36	40199.0
37	42945.0
38	34133.0
39	5120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.274163025519616	2.8458333444547357	34.890952427980025	33.98905120204563
2	32.724414324721806	17.919781771399133	35.07752506986465	14.278278834014408
3	12.55321590912124	17.964089437426097	55.26527213181264	14.217422521640025
4	11.348634603219512	2.8210103749336053	69.20403680205418	16.626318219792715
5	22.9054618540356	3.5184557443821345	55.373905987673936	18.202176413908337
6	14.272940560999112	15.749773790680976	58.55444905018777	11.422836598132138
7	57.12779558684969	1.04149706528441	39.16744294053431	2.663264407331584
8	55.99875084411442	11.520793907962835	29.228913154305452	3.251542093617294
9	52.915364350478974	2.8589121133422126	28.110278043950004	16.115445492228808
10	32.59175824029168	20.980480604719567	34.13211691885558	12.29564423613317
11	23.951496451383015	17.734009870466807	43.95640766255708	14.358086015593097
12	18.23046926088941	16.358870741726346	45.76928517855189	19.64137481883236
13	17.667815285077122	17.316556920670596	52.09860857913856	12.917019215113717
14	16.084750422390854	23.4171353225518	43.358787998494606	17.13932625656274
15	12.884455749720408	17.036564501018276	53.69448529706155	16.384494452199768
16	14.907127395216374	19.354442644260157	43.32061934643524	22.417810614088236
17	15.301625771046808	19.45133229948779	48.39384710652257	16.85319482294283
18	15.347001091676832	18.247017907236827	46.63034861591927	19.775632385167075
19	15.284810211048624	20.443450339380707	44.07198127333826	20.199758176232407
20	18.213119873589694	20.105270743861652	48.562803447456716	13.118805935091938
21	17.82182446156844	24.58648402655257	43.89875431399188	13.692937197887112
22	15.89337333479246	16.393302602675007	47.25893026347047	20.454393799062064
23	16.038574360808536	23.53724646539598	45.79277357981919	14.631405593976293
24	17.16815293084534	19.304262877916365	43.7983947813043	19.72918940993399
25	14.462716166692912	27.765425607161827	41.348394380933826	16.423463845211437
26	15.121192143129777	18.66660616623916	45.925429664249314	20.286772026381744
27	17.831433352995973	21.137692745020058	41.991389365626326	19.03948453635764
28	13.029389862085717	21.26287524722877	47.51062983614171	18.197105054543805
29	17.917112634891485	18.103685276776112	42.514540121125414	21.46466196720699
30	15.212476611691352	25.20065233696247	44.92316890562734	14.663702145718837
31	18.866791404312792	18.03402081392649	43.60755152100744	19.491636260753285
32	22.819782572140088	22.493347177254684	39.628135901754426	15.058734348850802
33	20.398875759702978	19.43745278964802	38.26794393745679	21.895727513192206
34	18.955139822715953	20.93830824789872	41.444216381558405	18.66233554782692
35	17.853587186009452	24.528296850685834	39.26006197734971	18.358053985955003
36	20.853963534257034	21.895193685890675	37.141034503927635	20.109808275924657
37	20.22404731845201	24.919859176357857	38.56742105361495	16.288672451575188
38	22.328661454732778	22.425818023611182	36.44198765257451	18.803532869081526
39	23.74837516315097	22.584898559467025	36.36698491670959	17.299741360672407
40	21.40514022308643	23.12272956575818	37.946046074634395	17.526084136520996
41	21.071498159630377	25.028493032219146	35.642047441232286	18.257961366918188
42	18.774973108449686	22.557673367089013	40.690185318147726	17.977168206313575
43	24.156753048821177	21.525518279581373	32.31016433873478	22.007564332862675
44	20.963665044721385	23.576749685709174	34.613095317533826	20.846489952035615
45	18.354317194844295	21.512973337995426	35.922840601836896	24.20986886532338
46	25.099492063322593	24.560326488777616	31.865753110211315	18.474428337688476
47	18.02361118154666	21.96165518493112	41.028364913666785	18.98636871985544
48	20.18828088924952	24.13780217961687	33.11811195959995	22.555804971533657
49	18.217924319303464	21.38805774943748	39.71861962936371	20.675398301895353
50	21.242322896119877	20.971939367895093	35.75708722471193	22.0286505112731
51	19.19642976300737	21.506834324027835	33.69784840906119	25.59888750390361
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23.0
1	421.0
2	819.0
3	9692.5
4	18566.0
5	13886.0
6	9206.0
7	8905.0
8	8604.0
9	8369.0
10	8134.0
11	7897.5
12	7661.0
13	7214.0
14	6767.0
15	6398.5
16	6030.0
17	5612.5
18	5195.0
19	4789.5
20	4384.0
21	4189.5
22	3995.0
23	3948.5
24	3902.0
25	3955.5
26	4397.0
27	4785.0
28	5219.5
29	5654.0
30	6109.0
31	6564.0
32	7141.0
33	7718.0
34	8375.0
35	9032.0
36	9750.0
37	10468.0
38	11466.5
39	12465.0
40	12900.5
41	13336.0
42	14243.0
43	15150.0
44	16636.0
45	18122.0
46	39279.0
47	60436.0
48	43143.0
49	25850.0
50	23872.5
51	21895.0
52	19500.0
53	17105.0
54	15628.5
55	14152.0
56	13379.0
57	12606.0
58	11254.5
59	9903.0
60	8508.0
61	7113.0
62	6155.5
63	5198.0
64	4246.5
65	3295.0
66	2741.0
67	2187.0
68	1761.0
69	1335.0
70	1125.5
71	916.0
72	758.0
73	600.0
74	603.5
75	506.0
76	405.0
77	344.0
78	283.0
79	207.0
80	131.0
81	82.5
82	34.0
83	18.5
84	3.0
85	5.0
86	7.0
87	4.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	374653.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.705814284085285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.31345995509223	36.41282608161838
2	5.122729878079088	4.47786161357719
3	2.201004198490046	2.8859004221309386
4	1.4968849120230119	2.6169029587810844
5	1.1518485913055871	2.517124030749363
6	0.9989079907089501	2.6194852277288496
7	0.8435259839046493	2.580689299743584
8	0.6910995941731102	2.416405641178933
9	0.6417261649585182	2.524244812822375
>10	3.44684624306179	23.11964658674847
>50	0.05678467727972149	1.6884472696839108
>100	0.02777511388682029	2.0472321983753314
>500	0.001234449506080902	0.3436781948517818
>1k	0.003703348518242706	3.215576203008822
>5k	0.001234449506080902	5.025416869618401
>10k+	0.001234449506080902	5.508562589382563
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	10232	2.7310604746258536	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG	10188	2.719316273992201	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC	9538	2.5458224009950543	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9091	2.4265119991031705	No Hit
GAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	2480	0.6619458538968058	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTC	2175	0.5805371904135292	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATGGTTGT	2124	0.5669245942245225	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTA	1737	0.46362901137852897	No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	1736	0.46336209772776404	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	1668	0.4452119694757549	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGT	703	0.18764029648768327	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	571	0.15240769458672423	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATG	467	0.12464867490718079	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTAT	446	0.11904348824111911	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08861533205392723	0.0
2	0.0	0.0	0.0	0.6512693078662122	0.0
3	0.0	0.0	0.0	0.7388169853170801	0.0
4	2.669136507648411E-4	0.0	0.0	1.5320843553901877	0.0
5	2.669136507648411E-4	0.0	0.0	4.7596042204386455	0.0
6	2.669136507648411E-4	0.0	0.0	5.004897865491535	0.0
7	2.669136507648411E-4	0.0	0.0	5.5867696241588884	0.0
8	2.669136507648411E-4	0.0	0.0	6.33999994661727	0.0
9	2.669136507648411E-4	0.0	0.0	6.452637507240032	0.0
10	2.669136507648411E-4	0.0	0.0	9.722863556410866	0.0
11	2.669136507648411E-4	0.0	0.0	9.932924599562796	0.0
12	2.669136507648411E-4	0.0	0.0	13.114268403028936	0.0
13	2.669136507648411E-4	0.0	0.0	13.378779830936894	0.0
14	2.669136507648411E-4	0.0	0.0	13.566953954726106	0.0
15	2.669136507648411E-4	0.0	0.0	14.217689435290788	0.0
16	2.669136507648411E-4	0.0	0.0	14.525707788273415	0.0
17	2.669136507648411E-4	0.0	0.0	14.686923633335379	0.0
18	2.669136507648411E-4	0.0	0.0	14.815576013004033	0.0
19	2.669136507648411E-4	0.0	0.0	15.649948085294927	0.0
20	2.669136507648411E-4	0.0	0.0	15.821840476387484	0.0
21	2.669136507648411E-4	0.0	0.0	15.964639279546674	0.0
22	2.669136507648411E-4	0.0	0.0	16.244097871897463	0.0
23	2.669136507648411E-4	0.0	0.0	16.386095934104357	0.0
24	2.669136507648411E-4	0.0	0.0	16.52889473726355	0.0
25	2.669136507648411E-4	0.0	0.0	16.634058715664896	0.0
26	2.669136507648411E-4	0.0	0.0	16.776323691522556	0.0
27	2.669136507648411E-4	0.0	0.0	16.954888923884234	0.0
28	2.669136507648411E-4	0.0	0.0	17.059519074984053	0.0
29	2.669136507648411E-4	0.0	0.0	17.192175159414177	0.0
30	2.669136507648411E-4	0.0	0.0	17.333639394319544	0.0
31	2.669136507648411E-4	0.0	0.0	17.466562392400434	0.0
32	2.669136507648411E-4	0.0	0.0	17.598150822227502	0.0
33	2.669136507648411E-4	0.0	0.0	17.737212834275983	0.0
34	2.669136507648411E-4	0.0	0.0	17.952345236792446	0.0
35	2.669136507648411E-4	0.0	0.0	18.115696391060528	0.0
36	2.669136507648411E-4	0.0	0.0	18.270506308504135	0.0
37	2.669136507648411E-4	0.0	0.0	18.431455239915334	0.0
38	2.669136507648411E-4	0.0	0.0	18.612956522435425	0.0
39	2.669136507648411E-4	0.0	0.0	18.841167693839367	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCTAT	25	3.8857546E-5	45.000004	31
CGTATGG	20	7.027193E-4	45.0	2
CACGGAT	20	7.027193E-4	45.0	15
CGACCCG	20	7.027193E-4	45.0	32
CGTCTTG	20	7.027193E-4	45.0	1
CCGCTTG	20	7.027193E-4	45.0	1
TGGCACG	20	7.027193E-4	45.0	22
TCTACGG	20	7.027193E-4	45.0	2
TTGCACG	20	7.027193E-4	45.0	1
TGCACGG	35	1.2089913E-7	45.0	2
CCCGATC	45	3.8380676E-10	45.0	41
CGTTTTT	7275	0.0	44.319584	1
CGATGAA	345	0.0	43.04348	19
GCGACCT	110	0.0	42.954544	11
GAATGAC	175	0.0	42.42857	1
CGGCTGT	1200	0.0	42.375	9
ACGGCTG	1205	0.0	42.19917	8
TACGGCT	1210	0.0	41.83884	7
TGATACC	1175	0.0	41.74468	4
CCGATGA	345	0.0	41.739132	18
>>END_MODULE