##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933319.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	244378
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.40538428172749	31.0	27.0	31.0	16.0	33.0
2	28.65392138408531	31.0	28.0	33.0	16.0	34.0
3	28.852924567677942	31.0	30.0	33.0	16.0	34.0
4	31.89584987191973	35.0	30.0	37.0	19.0	37.0
5	33.56869276285099	35.0	33.0	37.0	28.0	37.0
6	32.52494905433386	35.0	33.0	37.0	17.0	37.0
7	31.84358248287489	35.0	30.0	35.0	25.0	37.0
8	32.791147320953606	35.0	32.0	35.0	27.0	37.0
9	32.66355400240611	34.0	31.0	37.0	25.0	39.0
10	30.987576623100278	35.0	27.0	38.0	15.0	39.0
11	32.02778073312655	35.0	27.0	39.0	16.0	39.0
12	32.8505757474077	35.0	31.0	39.0	23.0	39.0
13	33.108393554247925	35.0	31.0	39.0	23.0	39.0
14	33.08434883663832	36.0	31.0	39.0	17.0	41.0
15	33.782865069687126	36.0	31.0	39.0	23.0	41.0
16	34.0129553396787	36.0	32.0	40.0	25.0	41.0
17	31.94002324268142	34.0	27.0	39.0	18.0	41.0
18	32.76071904999632	35.0	30.0	39.0	21.0	39.0
19	32.67682442773081	36.0	30.0	37.0	19.0	39.0
20	32.48958989761763	34.0	30.0	37.0	24.0	39.0
21	32.7860159261472	35.0	31.0	37.0	24.0	39.0
22	33.251937572121875	35.0	31.0	38.0	25.0	39.0
23	34.20559543003053	35.0	33.0	38.0	27.0	40.0
24	32.48958171357487	35.0	31.0	37.0	22.0	39.0
25	31.430992151503	35.0	29.0	37.0	18.0	39.0
26	31.03472898542422	33.0	27.0	37.0	20.0	39.0
27	32.67031402172045	35.0	31.0	38.0	22.0	39.0
28	32.60252968761509	35.0	31.0	38.0	21.0	39.0
29	32.93312818666165	35.0	31.0	38.0	22.0	40.0
30	31.72905498858326	35.0	30.0	38.0	18.0	40.0
31	32.20003028095819	35.0	30.0	38.0	21.0	40.0
32	32.10757924199396	35.0	30.0	38.0	19.0	40.0
33	32.24869259917014	35.0	31.0	38.0	18.0	40.0
34	31.552938480550623	35.0	30.0	38.0	15.0	40.0
35	31.0671623468561	35.0	30.0	38.0	10.0	40.0
36	30.5679357388963	35.0	28.0	38.0	10.0	40.0
37	30.65964612199134	35.0	28.0	38.0	9.0	40.0
38	30.698700374010755	35.0	28.0	38.0	9.0	40.0
39	30.184627912496214	35.0	26.0	38.0	8.0	40.0
40	30.14621610783295	35.0	25.0	38.0	8.0	40.0
41	29.532339244940214	34.0	24.0	38.0	8.0	40.0
42	30.086079761680676	35.0	25.0	38.0	8.0	40.0
43	30.162743782173518	35.0	25.0	38.0	8.0	40.0
44	30.04862549001956	35.0	25.0	38.0	7.0	40.0
45	30.313825303423386	35.0	26.0	38.0	7.0	40.0
46	30.05113389912349	35.0	25.0	38.0	7.0	40.0
47	30.113013446382244	35.0	25.0	38.0	7.0	40.0
48	29.809860134709343	35.0	25.0	38.0	7.0	40.0
49	29.629528844658683	34.0	25.0	38.0	7.0	40.0
50	29.759962026041624	35.0	25.0	38.0	7.0	40.0
51	28.15202677818789	33.0	23.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	9.0
11	14.0
12	22.0
13	25.0
14	55.0
15	109.0
16	218.0
17	464.0
18	789.0
19	1282.0
20	1902.0
21	2699.0
22	3624.0
23	4983.0
24	6631.0
25	9193.0
26	11341.0
27	12295.0
28	12262.0
29	13053.0
30	14809.0
31	17572.0
32	20359.0
33	22880.0
34	26270.0
35	27414.0
36	20494.0
37	11202.0
38	2366.0
39	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.427640785995465	2.9376621463470522	38.136411624614325	35.49828544304315
2	35.37143278036484	12.445064613017538	37.74685118955061	14.436651417067004
3	13.279018569593006	12.429924133923675	59.900645720973245	14.39041157551007
4	12.19135928766092	2.82267634566123	67.74095867876814	17.245005687909714
5	19.87331101817676	3.5273224267323573	58.82076127965693	17.77860527543396
6	14.82703025640606	11.390141502099208	60.98175776870258	12.80107047279215
7	57.91192333188748	1.0426470467881725	38.1294551882739	2.915974433050438
8	59.01022186939905	8.194682009018814	29.265727684161426	3.5293684374207173
9	55.015181399307636	2.755976397220699	29.434318964882273	12.794523238589397
10	30.947548469993208	21.725769095417753	34.87998101302081	12.446701421568227
11	22.126377988198612	18.36376433230487	45.64608925517027	13.863768424326247
12	18.385861247739157	17.20081185704114	46.728838111450294	17.684488783769407
13	17.105876961101245	18.17143932759905	51.657268657571464	13.065415053728241
14	15.620882403489675	20.354532732078994	46.098666819435465	17.925918044995868
15	13.08014633068443	17.64766059137893	52.9998608712732	16.27233220666345
16	15.05168222998797	17.47702329996972	45.78644558839176	21.68484888165056
17	15.362266652480994	18.157117252780527	49.188552160996494	17.292063933741993
18	15.24523484110681	16.963883819329073	48.373421502753935	19.41745983681019
19	15.570959742693697	19.678940002782575	45.62112792477228	19.128972329751452
20	17.79742857376687	19.61796888426945	49.34527657972485	13.239325962238826
21	18.387907258427518	22.396042196924437	44.649682049939024	14.566368494709018
22	16.084508425472013	17.089099673456694	47.586116589873065	19.240275311198225
23	15.469477612551048	22.595732840108358	46.01682639190107	15.917963155439525
24	15.956428156380689	18.768056044324776	45.798312450384245	19.477203348910294
25	14.758284297277168	23.489839510921605	43.46463265924101	18.28724353256021
26	14.527085089492509	19.90522878491517	46.54715236232394	19.02053376326838
27	15.513671443419621	21.075137696519327	44.507688908166855	18.903501951894196
28	13.089557979850886	20.827570403227785	48.66272741408801	17.420144202833317
29	16.981479511248967	18.04254065423238	44.73275008388644	20.243229750632217
30	15.348353779800144	22.733224758366138	46.268894908707004	15.649526553126714
31	17.900547512460204	18.84171242910573	44.795358010950245	18.462382047483818
32	20.172028578677295	21.94714745189829	42.28203848136903	15.59878548805539
33	18.645704605160855	19.66789154506543	41.010647439622225	20.675756410151486
34	17.721316976159883	21.419276694301452	42.1817839576394	18.67762237189927
35	16.7474158885006	23.990702927432093	40.40052705235332	18.861354131713984
36	19.24109371547357	21.916866493710565	39.09026180752768	19.751777983288186
37	19.495208242967863	24.104051919567226	39.8534237942859	16.54731604317901
38	21.35707796937531	22.34857474895449	38.542340145184916	17.752007136485283
39	21.796970267372675	22.141927669430146	38.65364312663169	17.407458936565483
40	20.590233163378045	22.682892895432484	39.136501649084614	17.590372292104853
41	19.889269901545966	23.49884195795039	38.26449189370565	18.34739624679799
42	18.7005376916089	22.04576516707723	40.726251953940206	18.527445187373658
43	22.094460221460196	21.377538076258908	35.18360899917341	21.34439270310748
44	20.251822995523327	23.132196842596304	36.434539934036614	20.18144022784375
45	18.22422640335873	21.38735892756304	37.85569895817136	22.532715710906874
46	22.672253639853015	24.08195500413294	34.45031876846525	18.795472587548797
47	18.01962533452275	21.529761271472882	41.38793181055578	19.062681583448594
48	19.499709466482255	22.859668218906776	36.49632945682508	21.14429285778589
49	18.063000761115976	21.096007005540596	40.26262593195787	20.578366301385557
50	20.73263550728789	20.39790815867222	37.3339662326396	21.53549010140029
51	19.103601797215788	21.155341315503033	35.954545826547395	23.78651106073378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	184.0
2	322.0
3	9150.5
4	17979.0
5	12040.5
6	6102.0
7	5722.0
8	5342.0
9	5401.0
10	5460.0
11	5197.0
12	4934.0
13	4728.5
14	4523.0
15	4187.5
16	3852.0
17	3594.5
18	3337.0
19	3049.0
20	2761.0
21	2605.0
22	2449.0
23	2369.5
24	2290.0
25	2317.0
26	2567.5
27	2791.0
28	3078.5
29	3366.0
30	3689.0
31	4012.0
32	4420.0
33	4828.0
34	5304.5
35	5781.0
36	6353.5
37	6926.0
38	7459.0
39	7992.0
40	8504.5
41	9017.0
42	9688.5
43	10360.0
44	11407.0
45	12454.0
46	20673.0
47	28892.0
48	24173.0
49	19454.0
50	18013.0
51	16572.0
52	14250.0
53	11928.0
54	10697.5
55	9467.0
56	8619.5
57	7772.0
58	7008.5
59	6245.0
60	5289.5
61	4334.0
62	3876.0
63	3418.0
64	2819.0
65	2220.0
66	1808.5
67	1397.0
68	1187.0
69	977.0
70	851.0
71	725.0
72	612.0
73	499.0
74	488.0
75	407.0
76	337.0
77	284.5
78	232.0
79	169.0
80	106.0
81	73.5
82	41.0
83	27.5
84	14.0
85	8.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	244378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.76228502124398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32895004973815	34.27853837491424
2	7.609499679293324	6.660181876685983
3	3.4802821499228296	4.569152981978119
4	2.2088847101698	3.866633690620748
5	1.6119176986380028	3.5270600879291965
6	1.239532684852409	3.2546869588595353
7	0.9750472294160083	2.9869206334014335
8	0.7944074891300892	2.7812069569857396
9	0.6706553694364263	2.641447028547411
>10	3.00297901687054	19.564089498281124
>50	0.03991996027303629	1.183584830364875
>100	0.028941971197951305	2.3313563927998904
>500	0.002993997020477722	1.0053919850553295
>1k	0.0049899950341295365	5.829701223509356
>5k	0.0	0.0
>10k+	9.97999006825907E-4	5.520047480067032
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12639	5.171905818035993	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG	4673	1.9122015893411028	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC	3708	1.5173215264876543	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	2915	1.1928242313137845	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATGGTTGT	1039	0.4251610210411739	No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	1013	0.4145217654617028	TruSeq Adapter, Index 22 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	972	0.3977444778171521	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTC	715	0.29257952843545654	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTA	615	0.2516593146682598	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	477	0.19518941966952835	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCATGGTTGTCGTATGCCG	431	0.17636612133661786	No Hit
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	284	0.11621340709883868	TruSeq Adapter, Index 23 (95% over 22bp)
GAATCTTTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	266	0.10884776862074327	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGT	255	0.10434654510635163	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.092021376719672E-4	0.0	0.0	0.14485755673587639	0.0
2	4.092021376719672E-4	0.0	0.0	0.8114478390035109	0.0
3	4.092021376719672E-4	0.0	0.0	0.9874047582024569	0.0
4	4.092021376719672E-4	0.0	0.0	1.708009722642791	0.0
5	4.092021376719672E-4	0.0	0.0	3.3648691780765865	0.0
6	4.092021376719672E-4	0.0	0.0	3.676681206982625	0.0
7	4.092021376719672E-4	0.0	0.0	4.089156961755968	0.0
8	4.092021376719672E-4	0.0	0.0	4.662858358772066	0.0
9	4.092021376719672E-4	0.0	0.0	4.839633682246356	0.0
10	4.092021376719672E-4	0.0	0.0	7.402466670485887	0.0
11	4.092021376719672E-4	0.0	0.0	8.036729983877436	0.0
12	4.092021376719672E-4	0.0	0.0	10.304119028717807	0.0
13	4.092021376719672E-4	0.0	0.0	10.597107759290935	0.0
14	4.092021376719672E-4	0.0	0.0	10.764062231461097	0.0
15	4.092021376719672E-4	0.0	0.0	11.246511551776347	0.0
16	4.092021376719672E-4	0.0	0.0	11.567326027711168	0.0
17	4.092021376719672E-4	0.0	0.0	11.888958907921335	0.0
18	4.092021376719672E-4	0.0	0.0	12.187676468421872	0.0
19	4.092021376719672E-4	0.0	0.0	12.998305903150039	0.0
20	4.092021376719672E-4	0.0	0.0	13.29415904868687	0.0
21	4.092021376719672E-4	0.0	0.0	13.635024429367618	0.0
22	4.092021376719672E-4	0.0	0.0	14.094558429973238	0.0
23	4.092021376719672E-4	0.0	0.0	14.468978385943089	0.0
24	4.092021376719672E-4	0.0	0.0	14.75828429727717	0.0
25	4.092021376719672E-4	0.0	0.0	15.012808026909132	0.0
26	4.092021376719672E-4	0.0	0.0	15.219864308571148	0.0
27	4.092021376719672E-4	0.0	0.0	15.433467824435915	0.0
28	4.092021376719672E-4	0.0	0.0	15.618017988525972	0.0
29	4.092021376719672E-4	0.0	0.0	15.827938685151691	0.0
30	4.092021376719672E-4	0.0	0.0	16.094329276776143	0.0
31	4.092021376719672E-4	0.0	0.0	16.315298431119004	0.0
32	4.092021376719672E-4	0.0	0.0	16.541587213251603	0.0
33	4.092021376719672E-4	0.0	0.0	16.773195623173937	0.0
34	4.092021376719672E-4	0.0	0.0	17.00275802240791	0.0
35	4.092021376719672E-4	0.0	0.0	17.275286646097438	0.0
36	4.092021376719672E-4	0.0	0.0	17.503621438918398	0.0
37	4.092021376719672E-4	0.0	0.0	17.7327746360147	0.0
38	4.092021376719672E-4	0.0	0.0	17.990571982748037	0.0
39	4.092021376719672E-4	0.0	0.0	18.281514702632805	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGCT	30	2.1584638E-6	45.000004	26
AGCGCTC	30	2.1584638E-6	45.000004	27
GAAATCG	20	7.0223754E-4	45.0	17
GGTACCA	20	7.0223754E-4	45.0	8
ACCGGGA	20	7.0223754E-4	45.0	4
TTGTGCG	35	1.2066812E-7	45.0	1
CACGGGA	50	2.1827873E-11	45.0	4
GGGTACC	40	6.7775545E-9	45.0	7
TACGGGA	20	7.0223754E-4	45.0	4
TGCGGAT	20	7.0223754E-4	45.0	4
CCGCTGA	20	7.0223754E-4	45.0	18
ACGGGAC	20	7.0223754E-4	45.0	5
ATCCGGC	25	3.881764E-5	45.0	6
CGAGCGA	20	7.0223754E-4	45.0	24
CCGACCC	20	7.0223754E-4	45.0	36
ATGCGCT	20	7.0223754E-4	45.0	12
CGATGCG	45	3.8198777E-10	45.0	10
CGCCGGT	25	3.881764E-5	45.0	28
AATCGAC	20	7.0223754E-4	45.0	19
GTACGGG	25	3.881764E-5	45.0	3
>>END_MODULE