##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933318.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	264132
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.45235336877016	31.0	28.0	33.0	25.0	34.0
2	28.691442157709023	31.0	28.0	33.0	16.0	34.0
3	28.684653885178623	31.0	28.0	34.0	16.0	34.0
4	33.14840307119168	35.0	32.0	37.0	28.0	37.0
5	34.22860539427256	35.0	33.0	37.0	30.0	37.0
6	33.74005421531658	35.0	33.0	37.0	28.0	37.0
7	33.61012675480442	35.0	32.0	37.0	29.0	37.0
8	33.60143413141914	35.0	33.0	37.0	28.0	37.0
9	34.74551739281874	37.0	34.0	39.0	28.0	39.0
10	34.54199794042373	37.0	32.0	39.0	27.0	39.0
11	34.82801402329139	37.0	34.0	39.0	27.0	39.0
12	34.953133281843925	37.0	34.0	39.0	27.0	39.0
13	35.109524783063016	37.0	34.0	39.0	27.0	39.0
14	35.9153567155816	38.0	34.0	40.0	27.0	41.0
15	36.240913634092045	38.0	34.0	40.0	29.0	41.0
16	36.15858358699438	38.0	34.0	40.0	30.0	41.0
17	35.44185104417488	37.0	33.0	40.0	27.0	41.0
18	35.24055396544152	37.0	33.0	39.0	27.0	40.0
19	34.55476049853861	37.0	33.0	38.0	27.0	40.0
20	33.875569790862144	35.0	32.0	38.0	27.0	40.0
21	34.205037632698804	35.0	33.0	38.0	27.0	40.0
22	34.76549603985886	35.0	33.0	39.0	29.0	40.0
23	35.10097224115215	35.0	33.0	39.0	30.0	40.0
24	34.55479457241076	35.0	33.0	39.0	27.0	40.0
25	33.528762134084474	35.0	33.0	38.0	24.0	40.0
26	33.58688837399482	35.0	32.0	38.0	24.0	40.0
27	34.352289764208805	35.0	33.0	38.0	27.0	40.0
28	34.04736646828101	35.0	33.0	39.0	25.0	40.0
29	34.1291513334242	35.0	33.0	39.0	24.0	40.0
30	33.33966350158254	35.0	33.0	39.0	21.0	40.0
31	33.214214862265834	35.0	33.0	39.0	21.0	40.0
32	32.60534126875956	35.0	31.0	39.0	18.0	40.0
33	31.90801947511093	35.0	30.0	39.0	14.0	40.0
34	31.367747944209714	35.0	30.0	39.0	10.0	40.0
35	30.856068178032196	35.0	26.0	39.0	8.0	41.0
36	30.236658186058484	35.0	23.0	39.0	7.0	40.0
37	30.104258476822196	35.0	23.0	39.0	7.0	40.0
38	29.75469840837157	35.0	22.0	39.0	7.0	40.0
39	29.383762664122486	35.0	21.0	39.0	7.0	40.0
40	29.191510305453335	35.0	21.0	39.0	7.0	40.0
41	28.805722138930534	35.0	20.0	39.0	7.0	40.0
42	28.975682613238835	35.0	20.0	39.0	7.0	40.0
43	28.909931397937395	35.0	20.0	39.0	7.0	40.0
44	28.955083064528342	35.0	18.0	39.0	7.0	40.0
45	29.014666908969758	35.0	20.0	39.0	7.0	40.0
46	28.80978450168855	35.0	20.0	39.0	7.0	40.0
47	28.62036027440825	34.0	20.0	39.0	7.0	40.0
48	28.562215104568928	34.0	20.0	38.0	7.0	40.0
49	28.70192555237533	35.0	20.0	39.0	7.0	40.0
50	28.70049066375903	35.0	20.0	39.0	7.0	40.0
51	27.390528220738116	33.0	18.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	16.0
11	14.0
12	15.0
13	33.0
14	47.0
15	75.0
16	165.0
17	275.0
18	561.0
19	1002.0
20	1659.0
21	2408.0
22	3650.0
23	5308.0
24	7810.0
25	11038.0
26	13911.0
27	14465.0
28	12921.0
29	11718.0
30	11964.0
31	13234.0
32	15216.0
33	17751.0
34	20071.0
35	23449.0
36	25334.0
37	26116.0
38	20780.0
39	3110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.560204746111793	2.5146517650265774	32.10478094286191	38.82036254599973
2	38.32553420259568	15.909848106249905	32.21230293943937	13.552314751715052
3	11.681659170414793	16.014719912770886	58.867914527584695	13.435706389229626
4	10.203988914633593	2.681992337164751	71.36734662971544	15.74667211848621
5	19.76966062423334	3.2536761922069264	60.09949570669211	16.877167476867626
6	13.391031756848848	12.991231656898824	63.157815031877995	10.459921554374327
7	52.26818408977329	1.077112958672179	44.19456938197568	2.460133569578847
8	51.42996683476444	9.306331682643526	36.02289764208805	3.2408038405039905
9	48.415943543379825	2.4711129283842928	34.921932972907484	14.191010555328395
10	29.905501794557267	16.27330274256811	41.644329350476276	12.176866112398347
11	23.26336831584208	15.456287008011147	47.38350521708842	13.896839459058349
12	17.679039268244665	14.15769388033256	50.98473490527463	17.178531946148137
13	17.21449881120046	15.011812275680342	56.004573470840334	11.76911544227886
14	14.862644435358078	20.26524616479639	49.31360077536989	15.558508624475643
15	12.373358775157875	15.572138173337574	57.440597882876745	14.613905168627808
16	14.912619447851833	17.60634834098102	47.58151227416595	19.899519937001198
17	15.157194130207625	17.709327154604516	51.283449184498664	15.8500295306892
18	15.599397271061438	16.806369542501475	50.224508957642385	17.369724228794695
19	15.047779140732665	19.09499795556767	47.071918586161466	18.785304317538202
20	16.901776384535005	19.15178774249239	51.72489512819348	12.221540744779126
21	16.477367376917602	21.944330864870594	49.03646661517726	12.541835143034543
22	14.84636469643966	14.496161010403888	51.517044508049004	19.140429785107447
23	15.476731331304045	20.637408568443053	50.53344539851287	13.35241470174004
24	17.23494313449336	17.7528659912468	47.66291096875804	17.349279905501795
25	14.453757969500098	24.862947314221678	45.41517120227765	15.268123514000575
26	14.628291914648736	16.272166946829618	49.96933351506065	19.130207623460997
27	17.21260581830297	17.75513758272379	46.84059485408811	18.19166174488513
28	12.464601032816924	19.286568836793723	50.48384898459861	17.76498114579074
29	16.282767707055562	17.019520542758922	49.42907334211682	17.268638408068693
30	14.541592839943664	20.23457967985704	47.84085230112217	17.38297517907713
31	17.149001256947283	20.494676903972255	44.431950691321006	17.924371147759455
32	18.608120182333078	23.385655657019974	42.95428043553981	15.051943725107146
33	16.115805733496888	19.094619356988176	40.07276664697954	24.7168082625354
34	17.939893689518875	20.978525888570864	42.5908257992216	18.490754622688655
35	18.539593839443913	22.988505747126435	37.80761134584223	20.66428906758742
36	17.072524343888663	27.69107870307271	37.64027077370406	17.596126179334576
37	16.990368452137567	25.561461693395728	38.454636318204535	18.993533536262174
38	19.34222282797995	25.290385110475068	34.51077491557252	20.856617145972468
39	20.111156542940652	24.387806096951525	35.501945996698616	19.999091363409207
40	21.575954447018912	21.73458725182863	37.35897202913694	19.330486272015506
41	17.870988748050216	27.2469825693214	34.38773037723563	20.494298305392757
42	19.98356882164978	23.128965820120243	39.06758741841201	17.819877939817967
43	20.08919782532976	23.63325912801175	35.77377977677828	20.503763269880213
44	20.334908303424047	22.547438402011117	34.513425105629004	22.604228188935835
45	18.367710084351764	22.574318901155483	34.52781185164993	24.53015916284282
46	22.207835476201293	25.205200430087988	32.730604394772314	19.85635969893841
47	15.87880302273106	22.80412823891085	40.686853542925505	20.630215195432587
48	18.656959399088336	24.91519391819242	32.94337679645026	23.484469886268986
49	18.375660654521226	22.17981918131843	38.49476776763134	20.949752396529007
50	18.399512365029608	21.98976269441037	36.72595520421608	22.884769736343948
51	18.00463404661306	21.749731195008557	34.557721139430285	25.687913618948098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23.0
1	388.5
2	754.0
3	8917.5
4	17081.0
5	12837.5
6	8594.0
7	8328.5
8	8063.0
9	7793.5
10	7524.0
11	7061.0
12	6598.0
13	6139.5
14	5681.0
15	5293.5
16	4906.0
17	4622.5
18	4339.0
19	4032.0
20	3725.0
21	3538.0
22	3351.0
23	3257.5
24	3164.0
25	3104.5
26	3203.5
27	3362.0
28	3581.0
29	3800.0
30	4059.0
31	4318.0
32	4651.0
33	4984.0
34	5398.0
35	5812.0
36	6293.0
37	6774.0
38	7133.0
39	7492.0
40	7643.0
41	7794.0
42	8211.0
43	8628.0
44	9484.5
45	10341.0
46	23727.0
47	37113.0
48	26208.5
49	15304.0
50	14199.5
51	13095.0
52	11865.5
53	10636.0
54	10288.5
55	9941.0
56	9552.5
57	9164.0
58	8549.5
59	7935.0
60	7094.5
61	6254.0
62	5462.5
63	4671.0
64	4033.5
65	3396.0
66	2810.0
67	2224.0
68	1841.5
69	1459.0
70	1296.5
71	1134.0
72	877.5
73	621.0
74	532.0
75	355.5
76	268.0
77	227.0
78	186.0
79	130.0
80	74.0
81	52.5
82	31.0
83	22.0
84	13.0
85	12.5
86	12.0
87	7.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	264132.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.19507495846852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.87661869491627	45.448676499482964
2	9.107302827571585	10.23571130129715
3	4.288465443569846	7.229719111746283
4	2.319211214793711	5.213129922394137
5	1.2900310936125288	3.6246697002155583
6	0.7190816722860662	2.4245309085225837
7	0.40794266979308463	1.6047058235446114
8	0.22935855865113233	1.0311057116613345
9	0.1646616672860317	0.8327857262332461
>10	0.5333762322729925	5.57175784807602
>50	0.041499419575004734	1.611417298140595
>100	0.014967003781149249	1.5441315461897656
>500	0.0020409550610658066	1.0261077172501438
>1k	0.002721273414754409	2.1485563975207933
>5k	0.002721273414754409	10.452994487724826
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8557	3.2396680447654957	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC	7708	2.918237850771584	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCG	6000	2.271591476988778	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGC	5077	1.9221449881120047	No Hit
GAACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCT	2006	0.7594687504732482	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTC	1408	0.5330667999333667	No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	1170	0.4429603380128118	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAACCGAT	1036	0.3922281283600624	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCT	979	0.370648009328669	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAACCGATCGTA	951	0.36004724910272135	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTT	754	0.2854633289415898	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCAACCGATCGT	484	0.18324171247709478	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	274	0.10373601078248755	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.785985794981297E-4	0.0	0.0	0.062090167037693274	0.0
2	3.785985794981297E-4	0.0	0.0	0.5739554465191646	0.0
3	3.785985794981297E-4	0.0	0.0	0.6765556615631578	0.0
4	3.785985794981297E-4	0.0	0.0	1.5973074069026092	0.0
5	3.785985794981297E-4	0.0	0.0	5.038011297381612	0.0
6	3.785985794981297E-4	0.0	0.0	5.473399663804462	0.0
7	3.785985794981297E-4	0.0	0.0	6.00003028788636	0.0
8	3.785985794981297E-4	0.0	0.0	6.719367588932807	0.0
9	3.785985794981297E-4	0.0	0.0	6.860584859085609	0.0
10	3.785985794981297E-4	0.0	0.0	9.6557781715203	0.0
11	3.785985794981297E-4	0.0	0.0	9.837126891099905	0.0
12	3.785985794981297E-4	0.0	0.0	12.235927490800055	0.0
13	3.785985794981297E-4	0.0	0.0	12.446049702421517	0.0
14	3.785985794981297E-4	0.0	0.0	12.593324549846288	0.0
15	3.785985794981297E-4	0.0	0.0	13.0972392591583	0.0
16	3.785985794981297E-4	0.0	0.0	13.37664501082792	0.0
17	3.785985794981297E-4	0.0	0.0	13.504232732118789	0.0
18	3.785985794981297E-4	0.0	0.0	13.601911165629307	0.0
19	3.785985794981297E-4	0.0	0.0	14.192524949646389	0.0
20	3.785985794981297E-4	0.0	0.0	14.314812290824285	0.0
21	3.785985794981297E-4	0.0	0.0	14.409461935698817	0.0
22	3.785985794981297E-4	0.0	0.0	14.598761225447882	0.0
23	3.785985794981297E-4	0.0	0.0	14.69871125043539	0.0
24	3.785985794981297E-4	0.0	0.0	14.808883437069344	0.0
25	3.785985794981297E-4	0.0	0.0	14.884981751548468	0.0
26	3.785985794981297E-4	0.0	0.0	14.986446170853966	0.0
27	3.785985794981297E-4	0.0	0.0	15.134099616858238	0.0
28	3.785985794981297E-4	0.0	0.0	15.231778050368755	0.0
29	3.785985794981297E-4	0.0	0.0	15.334378265412749	0.0
30	3.785985794981297E-4	0.0	0.0	15.46120878954462	0.0
31	3.785985794981297E-4	0.0	0.0	15.559265821634638	0.0
32	3.785985794981297E-4	0.0	0.0	15.680795965653537	0.0
33	3.785985794981297E-4	0.0	0.0	15.796268532400466	0.0
34	3.785985794981297E-4	0.0	0.0	15.943164781245741	0.0
35	3.785985794981297E-4	0.0	0.0	16.09081822725001	0.0
36	3.785985794981297E-4	0.0	0.0	16.218027349961382	0.0
37	3.785985794981297E-4	0.0	0.0	16.356973028637196	0.0
38	3.785985794981297E-4	0.0	0.0	16.52431360077537	0.0
39	3.785985794981297E-4	0.0	0.0	16.81999909136341	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	50	2.1827873E-11	45.0	26
CCGATGA	225	0.0	45.0	18
ATGCTAC	25	3.8826212E-5	45.0	3
TACGGTG	40	6.7811925E-9	45.0	7
CGCGACC	25	3.8826212E-5	45.0	10
AGGCACG	40	6.7811925E-9	45.0	10
CTGTTCG	20	7.02341E-4	45.0	1
GATGATC	20	7.02341E-4	45.0	9
ATACGGT	40	6.7811925E-9	45.0	6
TTCTTCG	30	2.1591313E-6	44.999996	1
GTATGAT	30	2.1591313E-6	44.999996	1
CGTTTTT	7265	0.0	44.4735	1
CGATGAA	230	0.0	44.02174	19
GATGAAT	240	0.0	43.124996	20
GCGACTG	95	0.0	42.63158	11
GATACCT	750	0.0	42.600002	5
TCTGGGT	85	0.0	42.35294	4
ACGGCTG	745	0.0	42.28188	8
TGATCTG	210	0.0	41.785713	4
CGGCTGT	765	0.0	41.764706	9
>>END_MODULE