##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933317.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186764
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.746241245636202	31.0	28.0	31.0	16.0	33.0
2	29.114053029491764	31.0	30.0	33.0	16.0	34.0
3	29.276193484825768	31.0	30.0	33.0	16.0	34.0
4	32.269414876528664	35.0	32.0	37.0	19.0	37.0
5	33.68160887537213	35.0	33.0	37.0	28.0	37.0
6	33.057687777087665	35.0	33.0	37.0	25.0	37.0
7	32.141831402197425	35.0	31.0	35.0	25.0	37.0
8	33.210950718553896	35.0	33.0	36.0	28.0	37.0
9	32.99367651153327	35.0	31.0	37.0	25.0	39.0
10	31.49902015377696	35.0	27.0	39.0	15.0	39.0
11	32.35663725343214	35.0	29.0	39.0	15.0	39.0
12	33.17065922768842	35.0	31.0	39.0	23.0	39.0
13	33.32322610353173	35.0	32.0	39.0	23.0	39.0
14	33.63346255166949	37.0	31.0	40.0	20.0	41.0
15	34.241791780000426	37.0	32.0	40.0	25.0	41.0
16	34.41104281339016	37.0	32.0	40.0	25.0	41.0
17	32.47027799790109	36.0	27.0	40.0	18.0	41.0
18	33.077745175729795	36.0	30.0	39.0	23.0	39.0
19	32.814926859566086	36.0	30.0	37.0	21.0	39.0
20	32.42411278404832	34.0	30.0	36.0	24.0	39.0
21	32.71308175023024	35.0	31.0	37.0	24.0	39.0
22	32.88308239275235	34.0	30.0	37.0	25.0	39.0
23	33.945637274849545	35.0	33.0	38.0	27.0	40.0
24	32.12373905035231	34.0	31.0	37.0	21.0	39.0
25	31.293959221263197	34.0	29.0	37.0	18.0	39.0
26	30.495100768884797	33.0	25.0	37.0	18.0	39.0
27	32.316683086676235	34.0	30.0	37.0	22.0	39.0
28	32.370531794135914	35.0	31.0	37.0	21.0	39.0
29	32.57454862821529	35.0	31.0	38.0	22.0	40.0
30	31.555781628151035	35.0	30.0	37.0	18.0	40.0
31	31.83674048531837	35.0	30.0	37.0	21.0	40.0
32	31.636659099183998	35.0	30.0	37.0	18.0	40.0
33	31.43140005568525	35.0	30.0	38.0	15.0	40.0
34	30.474802424450107	35.0	27.0	38.0	12.0	40.0
35	29.77578119980296	34.0	24.0	38.0	8.0	40.0
36	28.84165577948641	34.0	22.0	38.0	7.0	40.0
37	28.89009659249106	34.0	21.0	38.0	7.0	40.0
38	28.74338737658221	34.0	21.0	38.0	7.0	40.0
39	28.334502366623116	33.0	20.0	37.0	7.0	40.0
40	28.1545158595875	33.0	20.0	37.0	7.0	40.0
41	27.789922040650232	33.0	18.0	37.0	7.0	40.0
42	28.146141654708615	33.0	20.0	37.0	7.0	40.0
43	28.133992632413097	33.0	19.0	37.0	7.0	40.0
44	27.983208755434667	33.0	18.0	38.0	7.0	40.0
45	28.092373262513117	34.0	20.0	38.0	7.0	40.0
46	27.81291897796149	33.0	18.0	38.0	7.0	40.0
47	27.756291362361054	33.0	18.0	38.0	7.0	40.0
48	27.570104516930456	33.0	18.0	37.0	7.0	40.0
49	27.33169133237669	33.0	18.0	37.0	7.0	39.0
50	27.523725129039857	33.0	18.0	37.0	7.0	39.0
51	25.937247006917822	31.0	15.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	9.0
11	15.0
12	23.0
13	26.0
14	49.0
15	89.0
16	188.0
17	357.0
18	630.0
19	1131.0
20	1679.0
21	2441.0
22	3494.0
23	4664.0
24	6378.0
25	8804.0
26	11207.0
27	11826.0
28	11234.0
29	10728.0
30	11621.0
31	12755.0
32	14278.0
33	15789.0
34	17424.0
35	18299.0
36	13178.0
37	7007.0
38	1396.0
39	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.55749502045362	2.474245571951768	34.23090103017712	42.737358377417486
2	43.03934377074811	9.74384785076353	34.169325994302966	13.047482384185388
3	12.19078623289285	9.623374954488018	64.83476472981945	13.351074082799682
4	10.869332419524106	2.7901522777408925	70.38401404981688	15.95650125291812
5	16.311494720609968	3.1692403246878413	64.6296930886038	15.889571866098393
6	13.531515709665673	8.430960998907713	66.70129146944808	11.33623182197854
7	52.23490608468442	1.203658092566019	43.95868582810392	2.602749994645649
8	53.597052965239556	5.7644942280096805	37.61699256816089	3.0214602385898783
9	49.77993617613673	2.375725514553126	37.5291812126534	10.315157096656744
10	28.215287742819818	16.663275577734467	43.304919577648796	11.816517101796919
11	20.996016362896487	15.724122421880018	50.08352787475102	13.196333340472469
12	17.327215094986187	14.433188408901074	52.85493992418239	15.38465657193035
13	16.1053522092052	14.979867640444624	56.821443104666855	12.093337045683322
14	14.121565183868412	16.54119637617528	53.471761153113015	15.865477286843287
15	12.367479814096935	15.217600822428306	57.88321089717504	14.531708466299714
16	14.863142789831016	15.417853547792937	51.27219378466942	18.446809877706624
17	14.916150864192243	15.642200852412671	53.481934419909614	15.959713863485469
18	15.145317084662995	15.008781135550747	52.95720802724294	16.888693752543315
19	15.009852005739866	17.84819344199096	50.013385877363945	17.128568674905228
20	16.4367865327365	17.876036066907968	53.01128697179328	12.67589042856225
21	16.55833029920113	18.902465143175345	51.109421515923835	13.429783041699686
22	15.20635668544259	14.878134972478636	52.60382086483476	17.311687477244007
23	15.185474716754834	18.981174102075347	51.67323467049324	14.160116510676577
24	15.537255573879335	16.899402454434473	50.95575164378574	16.60759032790045
25	14.676811376924888	20.056863207042042	48.99177571694759	16.274549699085476
26	14.21098284465957	16.736094750594333	51.653959007089156	17.398963397656935
27	14.935426527596324	17.10875757640659	50.2184575185796	17.73735837741749
28	12.7920798440813	17.944036323916816	52.439977725900064	16.82390610610182
29	15.298451521706538	16.475873294639225	51.38838320018847	16.837291983465764
30	14.49369257458611	18.074147051894368	50.65644342592791	16.77571694759161
31	16.05823392088411	18.805551391060376	48.17148915208499	16.96472553597053
32	17.161765650767812	20.471825405324367	47.079201559187	15.287207384720825
33	15.53297209312287	18.47304619733996	44.93210683001006	21.061874879527103
34	16.59848793129297	20.19875350709987	45.8257480028271	17.377010558780064
35	16.53048767428412	21.85806686513461	42.11357649225761	19.497868968323658
36	16.421258914994326	24.34837548992311	41.512282881069154	17.71808271401341
37	16.471054378788203	24.200595403825147	41.32488059797391	18.003469619412733
38	18.06879270094879	23.264654858538048	39.8379773403868	18.82857510012636
39	18.06932813604335	22.411706752907413	40.10141140690926	19.417553704139983
40	20.080422351202586	20.829496048489002	40.826390524940564	18.263691075367845
41	17.3588057655651	23.890578484076162	39.73517380223169	19.015441948127048
42	18.317770019918186	21.789531173031204	42.27152984515217	17.62116896189844
43	18.34882525540254	21.956051487438693	40.701634147908585	18.993489109250177
44	18.954937782442013	21.943736480263862	38.6391381636718	20.462187573622327
45	17.423593412006596	22.648904499796537	38.042128033239806	21.885374054957058
46	18.963504743954935	24.360155062003383	36.22325501702684	20.45308517701484
47	15.6100747467392	22.435265897067957	40.96774538990384	20.986913966289006
48	17.559593926024284	23.708530551926497	36.05512839733567	22.676747124713543
49	17.1746160930372	22.056713285215565	38.785847379580645	21.982823242166585
50	16.884945706881414	21.538947548778136	38.93576920605684	22.64033753828361
51	17.145702597931077	20.8942836949305	37.92433231243709	24.03568139470133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24.0
1	170.5
2	317.0
3	9053.0
4	17789.0
5	12357.5
6	6926.0
7	6906.0
8	6886.0
9	6702.5
10	6519.0
11	5949.5
12	5380.0
13	4735.0
14	4090.0
15	3694.0
16	3298.0
17	3021.0
18	2744.0
19	2547.5
20	2351.0
21	2217.5
22	2084.0
23	1965.5
24	1847.0
25	1855.5
26	1895.5
27	1927.0
28	2080.0
29	2233.0
30	2514.0
31	2795.0
32	3065.0
33	3335.0
34	3533.0
35	3731.0
36	4010.0
37	4289.0
38	4616.5
39	4944.0
40	5217.5
41	5491.0
42	5774.5
43	6058.0
44	6697.0
45	7336.0
46	11867.0
47	16398.0
48	14011.0
49	11624.0
50	10989.5
51	10355.0
52	9144.5
53	7934.0
54	7562.5
55	7191.0
56	6750.0
57	6309.0
58	5923.5
59	5538.0
60	5123.5
61	4709.0
62	4200.5
63	3692.0
64	3183.5
65	2675.0
66	2300.5
67	1926.0
68	1681.0
69	1436.0
70	1216.0
71	996.0
72	810.0
73	624.0
74	547.0
75	373.5
76	277.0
77	245.5
78	214.0
79	147.0
80	80.0
81	53.5
82	27.0
83	21.0
84	15.0
85	12.5
86	10.0
87	6.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	186764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.64485397155092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.50274101593216	44.23918414169577
2	10.115269628375845	11.25725402707676
3	4.36726688286688	7.290477838557538
4	2.4017311016945	5.3457590573089036
5	1.2523054367943234	3.4842176579099706
6	0.778566691375538	2.599393790922322
7	0.426762427088092	1.6622993055103068
8	0.26685144465916305	1.1879127736125226
9	0.15391431628057364	0.7708085688207259
>10	0.653636122348531	6.8471826109441745
>50	0.05197106783195435	1.9498231184178583
>100	0.023986646691671238	2.6494459030640836
>500	9.994436121529683E-4	0.39541478528097473
>1k	0.0029983308364589048	3.52369631440261
>5k	0.0	0.0
>10k+	9.994436121529683E-4	6.797130106475489
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12222	6.544087725685892	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCG	2451	1.3123514167612602	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC	2111	1.1303034846115954	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGC	1774	0.9498618577456039	No Hit
GAACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCT	711	0.38069435223062265	No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	432	0.2313079608489859	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAACCGAT	422	0.22595360990340752	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTC	415	0.22220556424150262	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	283	0.15152813175986807	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTT	252	0.1349296438285751	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAACCGATCGTA	236	0.1263626823156497	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCT	232	0.12422094193741834	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	207	0.1108350645734724	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	196	0.10494527853333618	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTCAACCGATCGTATGCCG	194	0.1038744083442205	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07121286757619241	0.0
2	0.0	0.0	0.0	0.4358441669700799	0.0
3	0.0	0.0	0.0	0.5814825126898118	0.0
4	0.0	0.0	0.0	1.2400676789959522	0.0
5	0.0	0.0	0.0	2.7864042320789872	0.0
6	0.0	0.0	0.0	3.2243901394272987	0.0
7	0.0	0.0	0.0	3.6173994988327514	0.0
8	0.0	0.0	0.0	4.13034631941916	0.0
9	0.0	0.0	0.0	4.384142554239575	0.0
10	0.0	0.0	0.0	6.390417853547793	0.0
11	0.0	0.0	0.0	6.856246385813112	0.0
12	0.0	0.0	0.0	8.299779400741043	0.0
13	0.0	0.0	0.0	8.52037865969887	0.0
14	0.0	0.0	0.0	8.660127219378467	0.0
15	0.0	0.0	0.0	8.979781970829496	0.0
16	0.0	0.0	0.0	9.191278833179842	0.0
17	0.0	0.0	0.0	9.369578719667603	0.0
18	0.0	0.0	0.0	9.53609903407509	0.0
19	0.0	0.0	0.0	9.97140776595061	0.0
20	0.0	0.0	0.0	10.139534385641772	0.0
21	0.0	0.0	0.0	10.3258657985479	0.0
22	0.0	0.0	0.0	10.599473131866954	0.0
23	0.0	0.0	0.0	10.808828253839069	0.0
24	0.0	0.0	0.0	10.965175301449959	0.0
25	0.0	0.0	0.0	11.119380608682615	0.0
26	0.0	0.0	0.0	11.248420466471055	0.0
27	0.0	0.0	0.0	11.412263605405753	0.0
28	0.0	0.0	0.0	11.557901951125485	0.0
29	0.0	0.0	0.0	11.70514660212889	0.0
30	0.0	0.0	0.0	11.89790323616971	0.0
31	0.0	0.0	0.0	12.03711636075475	0.0
32	0.0	0.0	0.0	12.189179927609175	0.0
33	0.0	0.0	0.0	12.354093936732989	0.0
34	0.0	0.0	0.0	12.535070998693538	0.0
35	0.0	0.0	0.0	12.738536334625516	0.0
36	0.0	0.0	0.0	12.920584266775181	0.0
37	0.0	0.0	0.0	13.102632198924846	0.0
38	0.0	0.0	0.0	13.313058191086077	0.0
39	0.0	0.0	0.0	13.657342956886767	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	35	1.2046257E-7	45.000004	4
ATGATCC	35	1.2046257E-7	45.000004	3
CTCGTAA	20	7.0181023E-4	45.000004	33
AACTTAA	30	2.1557116E-6	45.000004	45
GAGAGTC	20	7.0181023E-4	45.000004	9
TACGGCC	20	7.0181023E-4	45.000004	7
CTTATCC	35	1.2046257E-7	45.000004	11
GCGATCT	20	7.0181023E-4	45.000004	9
TCGTAAC	20	7.0181023E-4	45.000004	34
CGTAACC	20	7.0181023E-4	45.000004	35
GGGCAAC	20	7.0181023E-4	45.000004	7
CACCGGT	55	1.8189894E-12	45.000004	16
TTACACG	75	0.0	45.000004	34
GTAACCA	20	7.0181023E-4	45.000004	36
TTCTTCG	25	3.878225E-5	45.0	1
TGATTGG	25	3.878225E-5	45.0	2
GGATTGT	50	2.1827873E-11	45.0	8
CGTTTTT	5935	0.0	44.545074	1
TACGGCT	260	0.0	42.40385	7
TGCGGGC	85	0.0	42.35294	4
>>END_MODULE