##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933316.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	799629
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.45467085360836	31.0	28.0	33.0	25.0	34.0
2	28.732318362640676	31.0	28.0	33.0	16.0	34.0
3	28.751554783530864	31.0	28.0	34.0	16.0	34.0
4	33.17842149296737	35.0	32.0	37.0	28.0	37.0
5	34.24389560658756	35.0	33.0	37.0	30.0	37.0
6	33.707974573208325	35.0	33.0	37.0	28.0	37.0
7	33.59530107087161	35.0	32.0	37.0	29.0	37.0
8	33.59692307307514	35.0	33.0	37.0	28.0	37.0
9	34.692073449062	37.0	34.0	39.0	28.0	39.0
10	34.44547784034846	37.0	32.0	39.0	27.0	39.0
11	34.892496395203274	37.0	34.0	39.0	27.0	39.0
12	35.03953833590327	37.0	34.0	39.0	27.0	39.0
13	35.334582662709835	37.0	34.0	39.0	30.0	39.0
14	36.07422317099555	38.0	34.0	40.0	29.0	41.0
15	36.46492310809138	38.0	34.0	40.0	30.0	41.0
16	36.295660862725086	38.0	34.0	40.0	30.0	41.0
17	35.599700611158426	38.0	33.0	40.0	27.0	41.0
18	35.35862006005285	37.0	33.0	39.0	27.0	40.0
19	34.71026938742842	37.0	33.0	38.0	27.0	40.0
20	34.067112373363145	35.0	33.0	38.0	27.0	40.0
21	34.38765852664173	35.0	33.0	38.0	27.0	40.0
22	34.870855109056826	35.0	33.0	39.0	29.0	40.0
23	35.1728864260801	35.0	34.0	39.0	30.0	40.0
24	34.60045596145212	35.0	33.0	39.0	27.0	40.0
25	33.55415699030426	35.0	33.0	38.0	24.0	40.0
26	33.59502344212128	35.0	32.0	38.0	24.0	40.0
27	34.38746093500861	35.0	33.0	39.0	27.0	40.0
28	34.11809726760785	35.0	33.0	39.0	25.0	40.0
29	34.14512104988688	35.0	33.0	39.0	24.0	40.0
30	33.34372315161156	35.0	33.0	39.0	21.0	40.0
31	33.22869480721685	35.0	33.0	39.0	21.0	40.0
32	32.573896144336935	35.0	31.0	39.0	16.0	40.0
33	31.808781322338234	35.0	30.0	39.0	12.0	40.0
34	31.198759674799188	36.0	28.0	40.0	8.0	40.0
35	30.64354594443173	36.0	24.0	40.0	7.0	41.0
36	30.0920189237759	35.0	22.0	40.0	7.0	41.0
37	30.056915144398214	36.0	21.0	40.0	7.0	41.0
38	29.789915073115157	35.0	21.0	39.0	7.0	40.0
39	29.49783712196531	35.0	20.0	39.0	7.0	40.0
40	29.24675443236801	35.0	20.0	39.0	7.0	40.0
41	29.010030901830724	35.0	18.0	39.0	7.0	40.0
42	29.151001276842134	35.0	18.0	39.0	7.0	40.0
43	29.098829582218755	35.0	18.0	39.0	7.0	40.0
44	29.175238766978186	35.0	18.0	39.0	7.0	40.0
45	29.308865736485295	35.0	20.0	39.0	7.0	40.0
46	29.069208345370164	35.0	18.0	39.0	7.0	40.0
47	29.009963370513077	35.0	18.0	39.0	7.0	40.0
48	28.83928922037595	35.0	18.0	39.0	7.0	40.0
49	28.954305058971098	35.0	18.0	39.0	7.0	40.0
50	28.928106659463324	35.0	18.0	39.0	7.0	40.0
51	27.921606144849672	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	31.0
10	33.0
11	69.0
12	89.0
13	138.0
14	174.0
15	238.0
16	440.0
17	986.0
18	1813.0
19	3416.0
20	5516.0
21	8080.0
22	11878.0
23	17637.0
24	25261.0
25	36241.0
26	44717.0
27	41281.0
28	34775.0
29	30346.0
30	30769.0
31	34350.0
32	41132.0
33	49736.0
34	59200.0
35	70813.0
36	82571.0
37	88925.0
38	69185.0
39	9775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.7547300060403	2.6010812514303505	34.56202814055018	38.08216060197917
2	38.346783320765006	14.611025863244079	33.01305980648526	14.029131009505658
3	11.361393846396266	14.706945345904163	59.20808274837456	14.723578059325012
4	10.107437324059031	2.6944995741775246	70.73342762706206	16.464635474701392
5	18.949662906172737	3.408580729313219	60.75204876261366	16.88970760190038
6	12.99615196547399	12.807064276058021	64.1862663810342	10.010517377433786
7	53.354493146196546	1.1591625616379597	43.018574864093225	2.4677694280722684
8	53.126262304143545	8.792202383855512	35.178188885095466	2.903346426905477
9	49.98880730938973	2.555560141015396	34.23775275784145	13.217879791753425
10	28.086275010035905	13.837292044185489	43.86909429247814	14.20733865330047
11	21.802986134820024	17.271134488619097	46.001583234224874	14.924296142336008
12	15.43503299655215	14.907413312923868	51.70472806764137	17.952825622882614
13	15.541207234855165	15.8902441007017	56.486195473150666	12.082353191292462
14	14.902285935102405	21.020998488048832	48.884420149844495	15.192295427004273
15	12.552446196923823	16.74914241479486	56.542846745178075	14.155564643103238
16	13.603308534332797	17.651060679390067	48.7167173776839	20.028913408593237
17	14.152438193212102	18.40053324729343	51.44423226271183	16.002796296782634
18	14.379168339317358	17.814886653685647	50.601216314065645	17.204728692931347
19	15.053606109833433	19.865587666280238	47.06082445734209	18.019981766544234
20	16.583065396577663	18.44492883574758	52.017748230742015	12.954257536932753
21	16.510781875094573	21.474833954246282	48.85453128888522	13.159852881773922
22	14.948432335495587	15.613865930325188	50.37135971806925	19.06634201610997
23	15.051605181903108	21.079400572015274	49.575740749772706	14.293253496308914
24	16.626835695053582	17.939444417348547	47.559055511993684	17.874664375604183
25	13.561414105791561	24.595781293574895	46.49918899889824	15.343615601735305
26	14.238102920229256	17.433084592979995	50.060340482898944	18.268472003891805
27	16.999258406085822	19.05446150652365	46.95077342117407	16.99550666621646
28	13.182988610968335	18.726434383945556	50.77041978217398	17.320157222912126
29	16.34045288502543	16.070077498439904	47.35083394924396	20.238635667290705
30	14.12705142009607	20.166727319794557	50.98551953468421	14.720701725425167
31	15.605612102612588	17.012389495628597	48.1244427103069	19.257555691451913
32	17.087799467002824	19.94462431952818	44.37070191301216	18.596874300456836
33	15.427904690800359	18.147165748115686	44.54015549711178	21.884774063972166
34	17.41132450173768	20.304916404982812	42.5348505369365	19.748908556343004
35	18.291482675090574	20.822531448959456	40.294686660939014	20.59129921501096
36	17.169337280163678	20.772508250701264	38.48634804390536	23.5718064252297
37	17.01201432164166	22.287460809950616	40.54217643432141	20.158348434086307
38	18.432798210169963	22.798072606171115	39.26445889281155	19.504670290847383
39	19.613595805054594	22.20367195286814	37.43461030052687	20.748121941550394
40	20.381076724330907	22.58059675174362	38.86764987262843	18.17067665129704
41	17.692079701961784	24.72158963719425	36.65424840769907	20.932082253144895
42	19.648987217822263	23.38171827184857	38.36341603418585	18.60587847614331
43	19.677000208846852	22.26495037073443	34.58691468168363	23.47113473873509
44	19.662243365360684	23.15036097990443	35.51159350148632	21.67580215324857
45	17.88479407325147	22.398512310083802	36.06985239404774	23.646841222616988
46	21.955681947503155	24.2595003432842	32.94452802487153	20.840289684341112
47	16.74363986298646	21.856761072947577	41.20285782531649	20.196741238749468
48	19.40649976426568	23.680731939436914	33.9362379303402	22.976530365957213
49	18.19831446833469	22.30134224746726	37.96698218798968	21.533361096208367
50	18.880005602598203	21.570753436906365	36.45890781850083	23.090333141994602
51	18.94040861449497	22.004454565804892	33.89822029966397	25.156916520036166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50.0
1	889.5
2	1729.0
3	20481.0
4	39233.0
5	30436.5
6	21640.0
7	21289.0
8	20938.0
9	20793.5
10	20649.0
11	20353.5
12	20058.0
13	19682.5
14	19307.0
15	18597.0
16	17887.0
17	17042.0
18	16197.0
19	15478.5
20	14760.0
21	13892.5
22	13025.0
23	12314.0
24	11603.0
25	11325.0
26	10738.5
27	10430.0
28	10753.0
29	11076.0
30	11974.5
31	12873.0
32	13985.0
33	15097.0
34	16723.0
35	18349.0
36	19593.0
37	20837.0
38	22424.5
39	24012.0
40	26427.5
41	28843.0
42	30789.0
43	32735.0
44	35216.5
45	37698.0
46	74857.0
47	112016.0
48	81823.5
49	51631.0
50	47792.5
51	43954.0
52	39312.5
53	34671.0
54	31931.0
55	29191.0
56	27192.5
57	25194.0
58	22751.5
59	20309.0
60	17634.0
61	14959.0
62	12544.5
63	10130.0
64	8336.0
65	6542.0
66	5422.0
67	4302.0
68	3467.5
69	2633.0
70	2092.5
71	1552.0
72	1275.5
73	999.0
74	828.5
75	511.0
76	364.0
77	277.0
78	190.0
79	156.0
80	122.0
81	98.5
82	75.0
83	55.5
84	36.0
85	24.5
86	13.0
87	7.0
88	1.0
89	2.0
90	3.0
91	5.0
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	799629.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.1304952152057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.23969491427641	41.72847127615315
2	6.00055619795032	6.016217075398427
3	2.7665089049277243	4.160593842639096
4	1.7880177940677977	3.585368698808735
5	1.279785726449281	3.2078146118127107
6	0.9939337207892069	2.989583378055296
7	0.797480265173545	2.798465643225235
8	0.6276455055458141	2.517134401008783
9	0.4793457649528563	2.162685651875845
>10	1.9528157026448452	14.525852138470219
>50	0.04301990974316112	1.4465667332330623
>100	0.027170355610964845	2.586785372838204
>500	0.0010063094670290736	0.3213460645426124
>1k	0.002012618934058147	3.026933632294835
>5k	0.0	0.0
>10k+	0.0010063094670290736	8.926181479643828
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	19990	2.4999093329531568	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18846	2.356842985934727	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	17904	2.2390383540366843	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	14037	1.755439084875611	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	4609	0.57639230193002	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	3911	0.4891018209694746	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTTGCT	3346	0.4184440534297781	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	3326	0.4159428935168684	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	3071	0.38405310462727094	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA	2938	0.36742039120642195	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTT	1630	0.20384453290213334	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGT	1170	0.1463178549052123	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.250579956454806E-4	0.0	0.0	0.05915243194031232	0.0
2	1.250579956454806E-4	0.0	0.0	0.547754020927205	0.0
3	1.250579956454806E-4	0.0	0.0	0.6554289551779637	0.0
4	1.250579956454806E-4	0.0	0.0	1.356504078766528	0.0
5	1.250579956454806E-4	0.0	0.0	4.304746326108733	0.0
6	1.250579956454806E-4	0.0	0.0	4.635899898577965	0.0
7	1.250579956454806E-4	0.0	0.0	5.1687720180233585	0.0
8	1.250579956454806E-4	0.0	0.0	5.824326031196968	0.0
9	1.250579956454806E-4	0.0	0.0	5.942630895077592	0.0
10	1.250579956454806E-4	0.0	0.0	8.643758543024328	0.0
11	1.250579956454806E-4	0.0	0.0	8.827093564640602	0.0
12	1.250579956454806E-4	0.0	0.0	11.038494101639635	0.0
13	1.250579956454806E-4	0.0	0.0	11.233459516850939	0.0
14	1.250579956454806E-4	0.0	0.0	11.376650921865014	0.0
15	1.250579956454806E-4	0.0	0.0	11.875382208499191	0.0
16	1.250579956454806E-4	0.0	0.0	12.121496343929497	0.0
17	1.250579956454806E-4	0.0	0.0	12.2625617630176	0.0
18	1.250579956454806E-4	0.0	0.0	12.383367786811133	0.0
19	1.250579956454806E-4	0.0	0.0	13.001154285299808	0.0
20	1.250579956454806E-4	0.0	0.0	13.15435032996552	0.0
21	1.250579956454806E-4	0.0	0.0	13.290288371232158	0.0
22	1.250579956454806E-4	0.0	0.0	13.523646591106626	0.0
23	1.250579956454806E-4	0.0	0.0	13.670214562003128	0.0
24	1.250579956454806E-4	0.0	0.0	13.794522209674737	0.0
25	1.250579956454806E-4	0.0	0.0	13.907199463751315	0.0
26	1.250579956454806E-4	0.0	0.0	14.031882285409859	0.0
27	1.250579956454806E-4	0.0	0.0	14.215967655000005	0.0
28	1.250579956454806E-4	0.0	0.0	14.325518459185448	0.0
29	1.250579956454806E-4	0.0	0.0	14.459705688513047	0.0
30	1.250579956454806E-4	0.0	0.0	14.600270875618568	0.0
31	2.501159912909612E-4	0.0	0.0	14.749590122419272	0.0
32	2.501159912909612E-4	0.0	0.0	14.91066482081065	0.0
33	2.501159912909612E-4	0.0	0.0	15.057983139681028	0.0
34	2.501159912909612E-4	0.0	0.0	15.257200526744278	0.0
35	2.501159912909612E-4	0.0	0.0	15.420151095070338	0.0
36	2.501159912909612E-4	0.0	0.0	15.587478693243991	0.0
37	2.501159912909612E-4	0.0	0.0	15.771313946842849	0.0
38	2.501159912909612E-4	0.0	0.0	15.961277042228334	0.0
39	2.501159912909612E-4	0.0	0.0	16.166247097091276	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTTC	30	2.1646683E-6	45.000004	15
CTCGTCG	20	7.0319965E-4	45.000004	1
TTCGCTA	20	7.0319965E-4	45.000004	21
CGTTTTT	20845	0.0	44.30919	1
TGATACC	2125	0.0	42.458824	4
TACGGCT	1935	0.0	41.860462	7
GATACCT	2145	0.0	41.748253	5
CGATGAA	535	0.0	41.635513	19
ACGGCTG	1925	0.0	41.61039	8
CGGCTGT	1950	0.0	41.192307	9
GAATCTG	2535	0.0	40.56213	1
TCAAGCG	50	1.0804797E-9	40.5	17
CCGATGA	545	0.0	40.458714	18
ATACCTG	2235	0.0	40.167786	6
TATGCGG	45	1.9270374E-8	40.0	2
ATGATAC	6865	0.0	39.919884	3
GAATGAT	7245	0.0	39.565216	1
AATCTGT	2600	0.0	39.46154	2
CGCGCAT	40	3.4564619E-7	39.375004	36
AATCGTT	40	3.4564619E-7	39.375004	22
>>END_MODULE