##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933315.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483433
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.517699453698857	31.0	28.0	31.0	16.0	33.0
2	28.78375286751215	31.0	30.0	33.0	16.0	34.0
3	28.982020259270673	31.0	30.0	33.0	16.0	34.0
4	32.14035864328666	35.0	30.0	37.0	19.0	37.0
5	33.67531384907526	35.0	33.0	37.0	28.0	37.0
6	32.749727469990674	35.0	33.0	37.0	17.0	37.0
7	32.13475290267731	35.0	32.0	35.0	25.0	37.0
8	33.02962147805383	35.0	32.0	35.0	28.0	37.0
9	32.8841038985754	35.0	31.0	37.0	25.0	39.0
10	31.483547875300196	35.0	27.0	39.0	15.0	39.0
11	32.399306625737175	35.0	30.0	39.0	17.0	39.0
12	33.15308843210952	35.0	31.0	39.0	23.0	39.0
13	33.51685962687694	35.0	32.0	39.0	25.0	39.0
14	33.54440636034363	36.0	32.0	40.0	19.0	41.0
15	34.19824463782985	37.0	32.0	40.0	25.0	41.0
16	34.287646892123625	37.0	32.0	40.0	25.0	41.0
17	32.22579550837448	35.0	27.0	39.0	18.0	41.0
18	32.95992412599057	36.0	30.0	39.0	22.0	39.0
19	32.809431710288706	36.0	30.0	37.0	20.0	39.0
20	32.48969557311975	34.0	30.0	37.0	24.0	39.0
21	32.87868432647337	35.0	31.0	37.0	24.0	39.0
22	33.20623747241086	35.0	31.0	38.0	25.0	39.0
23	34.18375245380435	35.0	33.0	38.0	27.0	40.0
24	32.40553913365451	35.0	31.0	37.0	22.0	39.0
25	31.55021481777206	35.0	30.0	37.0	18.0	39.0
26	30.90862229098965	33.0	26.0	37.0	20.0	39.0
27	32.625414483496165	35.0	31.0	38.0	22.0	39.0
28	32.58265571444233	35.0	31.0	38.0	21.0	39.0
29	32.811233407731784	35.0	31.0	38.0	22.0	40.0
30	31.770272199043095	35.0	30.0	38.0	18.0	40.0
31	31.955656730094965	35.0	30.0	38.0	20.0	40.0
32	31.801068607232025	35.0	30.0	38.0	18.0	40.0
33	31.570068654808424	35.0	30.0	38.0	15.0	40.0
34	30.541971689975654	35.0	27.0	38.0	10.0	40.0
35	29.946716504665588	35.0	25.0	38.0	8.0	40.0
36	29.392066739341335	35.0	23.0	38.0	7.0	40.0
37	29.572755686930766	35.0	23.0	38.0	7.0	40.0
38	29.432663885171266	35.0	23.0	38.0	7.0	40.0
39	29.085796790868642	34.0	22.0	38.0	7.0	40.0
40	28.780447342237704	34.0	22.0	38.0	7.0	40.0
41	28.41356506485904	33.0	21.0	38.0	7.0	40.0
42	28.84178159124429	34.0	21.0	38.0	7.0	40.0
43	28.899806177898487	34.0	21.0	38.0	7.0	40.0
44	28.903659866000044	34.0	21.0	38.0	7.0	40.0
45	29.1611247060089	35.0	22.0	38.0	7.0	40.0
46	28.937871432028842	35.0	20.0	38.0	7.0	40.0
47	28.9871481673779	35.0	20.0	38.0	7.0	40.0
48	28.740702434463515	34.0	20.0	38.0	7.0	40.0
49	28.5160839247631	34.0	20.0	38.0	7.0	40.0
50	28.69737688573184	34.0	20.0	38.0	7.0	40.0
51	27.132814681662197	32.0	18.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	16.0
10	27.0
11	23.0
12	36.0
13	74.0
14	109.0
15	196.0
16	454.0
17	877.0
18	1574.0
19	2740.0
20	4384.0
21	6095.0
22	8740.0
23	12038.0
24	16449.0
25	22393.0
26	26527.0
27	25762.0
28	24192.0
29	24336.0
30	27236.0
31	32042.0
32	38064.0
33	43915.0
34	49519.0
35	51394.0
36	39437.0
37	20538.0
38	4177.0
39	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.367732446895435	2.6344912324975747	37.49826759861242	37.499508721994566
2	38.96155206616015	11.602848791869814	35.15233755246332	14.283261589506715
3	12.184315096404259	11.4311600573399	61.49228538391049	14.89223946234535
4	10.892305655592398	2.8200391781280962	69.0507267811672	17.23692838511231
5	17.725930997677033	3.441221430891147	61.83586970686734	16.99697786456448
6	13.70531180122168	10.146183649026856	64.41885431900594	11.729650230745522
7	54.366996047022035	1.174723281199256	41.52798836653683	2.9302923052418843
8	55.74112648495241	6.989800034337747	33.61603365926612	3.6530398214437163
9	51.599704612635044	2.533546530749866	33.667126571831055	12.199622284784034
10	27.74055556819663	14.65332321128264	42.638380085761625	14.967741134759107
11	20.963401339999546	17.79957098501757	46.12055858826352	15.11646908671936
12	15.882242213502181	15.267472431546874	51.28094275732108	17.569342597629866
13	15.365728032633271	16.46805244987413	55.54130562042724	12.624913897065362
14	14.835354640663754	18.958159662248956	49.57998316209278	16.626502534994508
15	12.666284676470163	17.03214302705856	55.357826213766955	14.943746082704326
16	13.57582126168466	16.457502901125906	49.11435503989177	20.85232079729766
17	14.263610469289437	17.699660552754985	50.72760858278189	17.309120395173686
18	14.184178572832224	16.834597555400645	50.693270835875914	18.28795303589122
19	15.197142106558717	19.720623126679396	46.81289858160283	18.26933618515906
20	16.51749053126286	18.32022224382696	51.4553205925123	13.70696663239787
21	16.997805280152576	20.429304577883595	47.990931525154465	14.581958616809363
22	15.046138761731202	16.41302931326575	49.384299375508085	19.15653254949497
23	15.074477745623488	20.882521466263164	48.122490603661724	15.920510184451619
24	16.046277353842207	17.604921467917993	47.58446361750232	18.764337560737477
25	13.904098396261736	21.729588174576413	46.454420778060246	17.9118926511016
26	14.403650557574679	17.989876570279645	49.3611317390414	18.245341133104276
27	15.704141008164523	18.910996973727485	47.29093793762528	18.093924080482715
28	13.24588929593139	18.215140464138774	50.6138389394187	17.925131300511136
29	15.89299861614743	15.953813661872482	47.587359572060656	20.56582814991943
30	14.176938686436383	19.08537480891871	50.56150490347163	16.176181601173276
31	15.347938597489208	17.24665051827244	48.05650421051107	19.348906673727278
32	16.485635031121166	19.537350573916136	45.44559432227423	18.53142007268846
33	14.887688676610821	17.708968978121064	45.411670283162294	21.991672062105813
34	16.7566136362226	19.799848169239585	42.786694329927826	20.656843864609986
35	16.795295314966086	20.31201841827099	41.115728549768015	21.77695771699491
36	16.89499889333165	19.58968460986321	40.08683726597067	23.42847923083447
37	16.495150310384272	21.53411124188874	41.02760878963579	20.943129658091195
38	18.09268295710057	21.64436436900253	40.553292803759774	19.709659870137124
39	18.56782635856468	21.398001377646956	38.91397566984463	21.12019659394373
40	20.21707247953698	21.352907228095724	39.78421001462457	18.64581027774273
41	17.5678946203507	22.68835598728262	38.34719599199889	21.396553400367786
42	19.33546117042072	21.866111746612248	39.01636834887151	19.78205873409552
43	18.91637517505011	21.069517389172855	36.45861991216984	23.5554875236072
44	18.962089886292414	21.79350602875683	37.323476055627154	21.92092802932361
45	17.790469413548514	21.001255603154938	37.64430644991136	23.563968533385186
46	20.271888762248334	22.73117474396659	35.18460676039906	21.81232973338601
47	16.5609298496379	20.519492876986057	41.949763462568754	20.96981381080729
48	18.727517567067203	21.758134012365726	36.58293910428125	22.931409316285816
49	17.89989512507421	20.619817017042692	38.98223745586255	22.49805040202055
50	18.24658225648642	19.917548036646238	37.93183336677471	23.90403634009263
51	18.458607500936015	20.226794612697105	36.0556685207671	25.258929365599787
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37.0
1	297.0
2	557.0
3	15448.5
4	30340.0
5	21615.5
6	12891.0
7	12509.5
8	12128.0
9	12003.5
10	11879.0
11	11689.0
12	11499.0
13	11102.5
14	10706.0
15	10296.0
16	9886.0
17	9298.5
18	8711.0
19	8307.5
20	7904.0
21	7420.0
22	6936.0
23	6466.0
24	5996.0
25	5872.0
26	5578.5
27	5409.0
28	5559.5
29	5710.0
30	6263.5
31	6817.0
32	7556.0
33	8295.0
34	9246.5
35	10198.0
36	10946.5
37	11695.0
38	12770.0
39	13845.0
40	15557.0
41	17269.0
42	18965.0
43	20661.0
44	22390.5
45	24120.0
46	40020.0
47	55920.0
48	47268.0
49	38616.0
50	35391.5
51	32167.0
52	27768.5
53	23370.0
54	20785.0
55	18200.0
56	16664.5
57	15129.0
58	13619.0
59	12109.0
60	10694.5
61	9280.0
62	7900.5
63	6521.0
64	5465.0
65	4409.0
66	3779.5
67	3150.0
68	2554.0
69	1958.0
70	1602.5
71	1247.0
72	1043.5
73	840.0
74	697.5
75	426.5
76	298.0
77	229.5
78	161.0
79	148.5
80	136.0
81	105.5
82	75.0
83	56.5
84	38.0
85	20.5
86	3.0
87	4.0
88	5.0
89	5.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	483433.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.64349518903311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.5182906865553	44.26569529451466
2	7.304064353068396	7.836310819684254
3	3.208402592944083	5.163297871772318
4	1.9269201973495447	4.134669373446841
5	1.2632870935217744	3.3883567561851464
6	0.86574467553373	2.7864942202154803
7	0.6253692246633995	2.348289369622031
8	0.47701619433246145	2.047105274061143
9	0.3805077770434209	1.837059039649659
>10	1.3614668398405432	10.748874893079323
>50	0.03524848218002164	1.3425047667688756
>100	0.028198785740080445	3.0054038633160323
>500	0.001958249009727809	0.7067583779234625
>1k	0.001958249009727809	1.7549205501767786
>5k	0.0011749494058366852	4.47942787625034
>10k+	3.9164980194556176E-4	4.154831653333649
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19776	4.090742667546485	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	8733	1.8064550827105306	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	6600	1.3652357203583538	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	5988	1.23864113537967	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	2177	0.4503209338212327	TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1908	0.3946772355217786	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTTGCT	1792	0.37068218346699544	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	1416	0.29290511818597403	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTA	1060	0.2192651308454326	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	847	0.17520525077932206	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCTTCTTGCTCGTATGCCG	747	0.15451986107692275	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTT	674	0.13941952659417126	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	585	0.12100952975903591	TruSeq Adapter, Index 27 (95% over 24bp)
GAATCTTTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	511	0.10570234137926042	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1443840201227471	0.0
2	0.0	0.0	0.0	0.738261558478631	0.0
3	0.0	0.0	0.0	0.970765338733599	0.0
4	0.0	0.0	0.0	1.795491826168259	0.0
5	0.0	0.0	0.0	3.7262661009902094	0.0
6	0.0	0.0	0.0	4.266361626119855	0.0
7	0.0	0.0	0.0	4.801699511617949	0.0
8	0.0	0.0	0.0	5.506450738778693	0.0
9	0.0	0.0	0.0	5.7720511425575	0.0
10	0.0	0.0	0.0	8.23113027037873	0.0
11	0.0	0.0	0.0	8.911265883793618	0.0
12	0.0	0.0	0.0	10.86334611000904	0.0
13	0.0	0.0	0.0	11.156458082092037	0.0
14	0.0	0.0	0.0	11.33166333287136	0.0
15	0.0	0.0	0.0	11.790465276470576	0.0
16	0.0	0.0	0.0	12.101159829800613	0.0
17	0.0	0.0	0.0	12.414750337688988	0.0
18	0.0	0.0	0.0	12.752542751529168	0.0
19	0.0	0.0	0.0	13.47177375148159	0.0
20	0.0	0.0	0.0	13.773987295033645	0.0
21	0.0	0.0	0.0	14.122122403725024	0.0
22	0.0	0.0	0.0	14.580717493427217	0.0
23	0.0	0.0	0.0	14.971257651008516	0.0
24	0.0	0.0	0.0	15.283400181617722	0.0
25	0.0	0.0	0.0	15.554999348410224	0.0
26	0.0	0.0	0.0	15.804672002118185	0.0
27	0.0	0.0	0.0	16.062411957810077	0.0
28	0.0	0.0	0.0	16.319738205707928	0.0
29	0.0	0.0	0.0	16.596508719926028	0.0
30	0.0	0.0	0.0	16.88569046796557	0.0
31	0.0	0.0	0.0	17.155221095787834	0.0
32	0.0	0.0	0.0	17.42289003853688	0.0
33	0.0	0.0	0.0	17.69531662091748	0.0
34	0.0	0.0	0.0	17.96981174226832	0.0
35	0.0	0.0	0.0	18.2610620292781	0.0
36	0.0	0.0	0.0	18.53245434217358	0.0
37	0.0	0.0	0.0	18.832185638961345	0.0
38	0.0	0.0	0.0	19.128607273396728	0.0
39	0.0	0.0	0.0	19.424408346141036	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	20	7.029225E-4	45.000004	2
ACGGGGC	20	7.029225E-4	45.000004	5
CGAGCAA	20	7.029225E-4	45.000004	24
CCGAACA	20	7.029225E-4	45.000004	34
TGTACGG	30	2.162882E-6	45.000004	2
TTGTACG	25	3.887441E-5	45.0	1
CCGGTGA	25	3.887441E-5	45.0	18
CGTTTTT	13400	0.0	44.395523	1
TACGGCT	795	0.0	42.16981	7
TTATACG	70	0.0	41.785717	26
ACGGCTG	820	0.0	40.884148	8
GAATGAT	3290	0.0	40.69149	1
ATACGGA	50	1.0786607E-9	40.5	28
ATGCTAC	95	0.0	40.263157	3
CGATGAA	95	0.0	40.263157	19
CCGATGA	140	0.0	40.178574	18
CACCGGT	135	0.0	40.000004	16
GATACCT	1280	0.0	39.726566	5
CACGACC	85	0.0	39.705883	27
AATGATA	3140	0.0	39.697453	2
>>END_MODULE