##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933314.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 600529 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.474435039773265 31.0 28.0 33.0 25.0 34.0 2 28.821986948174025 31.0 28.0 33.0 16.0 34.0 3 28.81572913214849 31.0 28.0 34.0 16.0 34.0 4 33.17454943891136 35.0 32.0 37.0 28.0 37.0 5 34.22420232828057 35.0 33.0 37.0 30.0 37.0 6 33.7682493268435 35.0 33.0 37.0 28.0 37.0 7 33.58031502225538 35.0 32.0 37.0 28.0 37.0 8 33.6291985899099 35.0 33.0 37.0 28.0 37.0 9 34.72532550468004 37.0 34.0 39.0 28.0 39.0 10 34.494773774455524 37.0 32.0 39.0 27.0 39.0 11 34.64935914835087 37.0 33.0 39.0 27.0 39.0 12 34.74509141107257 37.0 33.0 39.0 27.0 39.0 13 34.835250254359075 37.0 34.0 39.0 27.0 39.0 14 35.70998236554771 38.0 34.0 40.0 27.0 41.0 15 36.04107212141295 38.0 34.0 40.0 27.0 41.0 16 35.89713902242856 38.0 34.0 40.0 27.0 41.0 17 35.27295101485524 37.0 33.0 40.0 27.0 41.0 18 35.00941503241309 37.0 33.0 39.0 27.0 40.0 19 34.27267459190147 37.0 33.0 37.0 27.0 40.0 20 33.67625376959314 35.0 32.0 37.0 27.0 39.0 21 33.945781136298166 35.0 33.0 38.0 27.0 40.0 22 34.46141318737313 35.0 33.0 38.0 28.0 40.0 23 34.885202879461275 35.0 33.0 39.0 29.0 40.0 24 34.32158979832781 35.0 33.0 38.0 27.0 40.0 25 33.411422262705045 35.0 33.0 38.0 24.0 40.0 26 33.36942595611536 35.0 32.0 38.0 24.0 40.0 27 34.09291974242709 35.0 33.0 38.0 25.0 40.0 28 33.86999795180582 35.0 33.0 38.0 24.0 40.0 29 33.951579357533106 35.0 33.0 39.0 24.0 40.0 30 33.18382792504608 35.0 33.0 38.0 21.0 40.0 31 32.99907748002178 35.0 32.0 38.0 21.0 40.0 32 32.401088040710775 35.0 31.0 38.0 18.0 40.0 33 31.806475623991513 35.0 31.0 39.0 13.0 40.0 34 31.05832690844239 35.0 29.0 39.0 10.0 40.0 35 30.51782345232287 35.0 25.0 39.0 8.0 40.0 36 30.01391606400357 35.0 23.0 39.0 7.0 40.0 37 29.899891595576566 35.0 23.0 39.0 7.0 40.0 38 29.64235032779433 35.0 22.0 39.0 7.0 40.0 39 29.23341587167314 35.0 21.0 38.0 7.0 40.0 40 28.921324365684256 35.0 21.0 38.0 7.0 40.0 41 28.476931172349712 34.0 18.0 38.0 7.0 40.0 42 28.646997896854273 34.0 18.0 38.0 7.0 40.0 43 28.635123366232104 34.0 18.0 38.0 7.0 40.0 44 28.672633628017966 35.0 18.0 38.0 7.0 40.0 45 28.79638951657622 35.0 20.0 39.0 7.0 40.0 46 28.680205285673132 35.0 20.0 38.0 7.0 40.0 47 28.61423511603936 34.0 20.0 38.0 7.0 40.0 48 28.466651901906484 34.0 20.0 38.0 7.0 40.0 49 28.620544553218913 34.0 20.0 38.0 7.0 40.0 50 28.62600307395646 34.0 20.0 38.0 7.0 40.0 51 27.5494172637791 33.0 18.0 37.0 7.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 12.0 9 35.0 10 45.0 11 47.0 12 64.0 13 80.0 14 115.0 15 208.0 16 365.0 17 701.0 18 1396.0 19 2389.0 20 3914.0 21 5641.0 22 8138.0 23 11692.0 24 17404.0 25 24725.0 26 31514.0 27 32965.0 28 31064.0 29 29044.0 30 29624.0 31 32661.0 32 37160.0 33 42111.0 34 45944.0 35 53115.0 36 56849.0 37 55634.0 38 40350.0 39 5518.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.705051712739934 2.500628612440032 31.774485495288317 41.01983417953171 2 40.01155647770549 13.978342428092565 31.717202666315863 14.29289842788608 3 12.238876057609207 14.247771548085106 58.82480279886567 14.688549595440021 4 10.933193900710874 2.9014418953955596 69.37916403704068 16.78620016685289 5 19.090168834477602 3.2428075913069976 59.979284930452984 17.687738643762415 6 13.920393519713453 12.006414344686101 62.94067397244763 11.13251816315282 7 52.04827743539446 1.3125094708165634 43.96340559739829 2.675807496390682 8 51.22816716594869 8.490514196649954 37.11494365800819 3.166374979393168 9 48.19717282595845 2.5291035070745957 36.290337352567484 12.98338631439947 10 30.39769936172941 14.883377821887036 42.254911919324464 12.464010897059092 11 25.00711872365864 14.288735431594477 46.90214793956661 13.801997905180267 12 19.702795368749886 13.070476196819806 50.79454947221534 16.43217896221498 13 18.34366033946737 13.52374323304953 55.99213360220738 12.140462825275716 14 15.132991079531546 17.898552775969186 51.34190022463528 15.626555919863986 15 13.302438350187916 13.299440992857964 58.13874101000951 15.259379646944609 16 16.55074109659983 15.671016720258304 48.624462765328566 19.153779417813293 17 16.556569291408074 15.92562557345274 51.81065360706977 15.707151528069419 18 17.59282232831387 14.841414819267678 50.713454304454906 16.852308547963545 19 16.51910232478365 17.512892799515097 47.52976126048867 18.43824361521259 20 18.178805686319894 18.46338811281387 51.20668610508402 12.151120095782218 21 17.527546546461537 20.32324833605038 49.5714611617424 12.577743955745685 22 15.72530219190081 13.451140577723974 52.22562107741674 18.597936152958475 23 16.2000502889952 18.801090371988696 51.671776050781894 13.327083288234206 24 18.074397739326496 16.288305810377185 48.61813501096533 17.01916143933099 25 16.40836662342701 22.06837638149032 45.91951429489667 15.603742700186002 26 15.567940932078217 14.390978620516245 49.92681452519362 20.114265922211917 27 17.477091031407312 15.256382289614656 48.67874823697107 18.587778442006964 28 12.81103826792711 17.649272558028006 50.48215823049345 19.057530943551434 29 17.41314740836829 15.7865814973132 47.147598200919525 19.652672893398986 30 16.984525310184853 17.385338593140382 51.63347648489914 13.996659611775616 31 18.750468337082804 14.935165495754577 49.04375975181881 17.270606415343806 32 20.608163802247685 17.505399406190207 46.95993032809406 14.926506463468042 33 21.750156944960196 16.109630009541586 41.84044400853248 20.299769036965742 34 21.17799473464229 19.000414634430644 42.703682919559256 17.11790771136781 35 17.159704194135504 24.07943663003785 41.635291551282286 17.12556762454436 36 19.73326850160442 21.926168428169166 37.581199242667715 20.7593638275587 37 18.95262343700304 22.622054888273503 41.688244864111475 16.737076810611978 38 23.065330733403382 22.45969803290099 36.42222107508547 18.05275015861016 39 24.655595316795694 20.366043937928062 36.11415934950685 18.8642013957694 40 23.601025096206843 23.11911664549089 36.32813735889524 16.95172089940702 41 21.276574486827446 23.006715745617615 33.75174221394804 21.964967553606904 42 19.256522166290054 23.467476175172223 38.96198185266657 18.31401980587116 43 21.799280301201108 19.553760101510502 35.13835301875513 23.508606578533257 44 23.065830292958374 20.65212504308701 34.94369131215978 21.338353351794833 45 19.700630610678253 20.13091790737833 35.440919589228834 24.72753189271459 46 22.360618721160844 21.938157857488978 34.42248417645109 21.278739244899082 47 17.405320975340075 20.896576185329934 42.006131260938275 19.69197157839172 48 19.866484382935713 23.226688469665913 34.807977633053525 22.098849514344852 49 19.12130804673879 21.149519840007727 39.12050875145081 20.608663361802677 50 19.025559132031926 20.065808645377658 37.31826439689008 23.59036782570034 51 19.04454239512163 20.268296785001226 35.39079711387793 25.29636370599921 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 60.0 1 1110.5 2 2161.0 3 23789.5 4 45418.0 5 32785.0 6 20152.0 7 18874.5 8 17597.0 9 16934.0 10 16271.0 11 15400.0 12 14529.0 13 13522.5 14 12516.0 15 11593.5 16 10671.0 17 9918.5 18 9166.0 19 8448.5 20 7731.0 21 7221.0 22 6711.0 23 6681.5 24 6652.0 25 6555.5 26 6722.5 27 6986.0 28 7281.0 29 7576.0 30 8012.0 31 8448.0 32 8836.5 33 9225.0 34 10067.5 35 10910.0 36 11281.0 37 11652.0 38 12175.5 39 12699.0 40 13331.5 41 13964.0 42 14556.0 43 15148.0 44 17049.0 45 18950.0 46 45874.0 47 72798.0 48 52156.0 49 31514.0 50 30485.5 51 29457.0 52 27796.5 53 26136.0 54 26774.5 55 27413.0 56 27194.5 57 26976.0 58 25843.0 59 24710.0 60 22144.0 61 19578.0 62 17617.0 63 15656.0 64 13611.5 65 11567.0 66 9780.0 67 7993.0 68 6713.5 69 5434.0 70 4460.5 71 3487.0 72 2883.5 73 2280.0 74 1994.0 75 1425.0 76 1142.0 77 863.5 78 585.0 79 415.0 80 245.0 81 176.0 82 107.0 83 78.5 84 50.0 85 36.0 86 22.0 87 15.5 88 9.0 89 6.5 90 4.0 91 4.5 92 5.0 93 2.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 600529.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.0591554471152 #Duplication Level Percentage of deduplicated Percentage of total 1 79.29668515908128 41.281184591375656 2 9.000507725785706 9.371176615992788 3 4.609986352550338 7.199759884094932 4 2.7293111438773994 5.6834253241062935 5 1.5858684354542993 4.127948569999438 6 0.944664081835396 2.950704856894514 7 0.5453080753393131 1.9871794502459559 8 0.31478785162886014 1.3110071760648212 9 0.19725066750369086 0.924183284545969 >10 0.6774223164737074 6.09971221636834 >50 0.04764555876449863 1.7506136447832825 >100 0.0447284818932976 3.935041882296357 >500 0.0022688360380658077 0.736691945375663 >1k 0.0022688360380658077 2.6621137934475114 >5k 3.2411943400940105E-4 1.5143862138677453 >10k+ 9.723583020282032E-4 8.464870550540743 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22537 3.7528578969541857 No Hit GAATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC 15051 2.506290287396612 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCG 12579 2.094653214082917 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGC 8975 1.4945156686854424 No Hit GAACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT 3396 0.5655014162513383 No Hit GAATGATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTC 2685 0.44710580171815184 No Hit GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC 2199 0.36617715380939136 TruSeq Adapter, Index 14 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTGGACT 2151 0.3581842009295138 No Hit GAATGACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCT 2012 0.33503794154820166 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTA 1766 0.29407405803882913 No Hit GAATGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTT 1568 0.2611031274093341 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGT 812 0.1352141195512623 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 720 0.11989429319816361 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 645 0.1074053043233549 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 601 0.10007843085013379 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.6651985166411614E-4 0.0 0.0 0.04396124083932666 0.0 2 1.6651985166411614E-4 0.0 0.0 0.4695859816928075 0.0 3 1.6651985166411614E-4 0.0 0.0 0.5668335750646514 0.0 4 1.6651985166411614E-4 0.0 0.0 1.2500645264425199 0.0 5 1.6651985166411614E-4 0.0 0.0 4.236265026335115 0.0 6 1.6651985166411614E-4 0.0 0.0 4.666219283331863 0.0 7 1.6651985166411614E-4 0.0 0.0 5.194753292513767 0.0 8 1.6651985166411614E-4 0.0 0.0 5.86482917561017 0.0 9 1.6651985166411614E-4 0.0 0.0 6.052996607990622 0.0 10 1.6651985166411614E-4 0.0 0.0 8.760109836494157 0.0 11 1.6651985166411614E-4 0.0 0.0 8.989574192087309 0.0 12 1.6651985166411614E-4 0.0 0.0 10.907216803851272 0.0 13 1.6651985166411614E-4 0.0 0.0 11.083727846615234 0.0 14 1.6651985166411614E-4 0.0 0.0 11.221273244089794 0.0 15 1.6651985166411614E-4 0.0 0.0 11.63291031740349 0.0 16 1.6651985166411614E-4 0.0 0.0 11.847054846643543 0.0 17 1.6651985166411614E-4 0.0 0.0 11.969446937616668 0.0 18 1.6651985166411614E-4 0.0 0.0 12.059700697218618 0.0 19 1.6651985166411614E-4 0.0 0.0 12.58806818654886 0.0 20 1.6651985166411614E-4 0.0 0.0 12.70579772167539 0.0 21 1.6651985166411614E-4 0.0 0.0 12.791055885727417 0.0 22 1.6651985166411614E-4 0.0 0.0 12.973728163002953 0.0 23 1.6651985166411614E-4 0.0 0.0 13.067312319638186 0.0 24 1.6651985166411614E-4 0.0 0.0 13.160396916718426 0.0 25 1.6651985166411614E-4 0.0 0.0 13.235663889670606 0.0 26 1.6651985166411614E-4 0.0 0.0 13.331079764674145 0.0 27 1.6651985166411614E-4 0.0 0.0 13.48660930612843 0.0 28 1.6651985166411614E-4 0.0 0.0 13.57153443047713 0.0 29 1.6651985166411614E-4 0.0 0.0 13.676941496580515 0.0 30 1.6651985166411614E-4 0.0 0.0 13.801664865476939 0.0 31 1.6651985166411614E-4 0.0 0.0 13.901576776475407 0.0 32 1.6651985166411614E-4 0.0 0.0 14.01697503367864 0.0 33 1.6651985166411614E-4 0.0 0.0 14.124713377705323 0.0 34 1.6651985166411614E-4 0.0 0.0 14.295229705809378 0.0 35 1.6651985166411614E-4 0.0 0.0 14.450093167857005 0.0 36 1.6651985166411614E-4 0.0 0.0 14.5833090491883 0.0 37 1.6651985166411614E-4 0.0 0.0 14.722020085624507 0.0 38 1.6651985166411614E-4 0.0 0.0 14.911686196669937 0.0 39 1.6651985166411614E-4 0.0 0.0 15.288354101134166 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATCA 25 3.888572E-5 45.000004 10 CGACGTC 20 7.030592E-4 45.0 45 AGGGATA 20 7.030592E-4 45.0 6 CGTTTTT 16795 0.0 44.169395 1 GATACCT 1465 0.0 42.84983 5 TACGGCT 1155 0.0 42.272728 7 TGATACC 1505 0.0 42.159466 4 CCGATGA 425 0.0 41.823532 18 ATACCTG 1520 0.0 41.595394 6 GAATCTG 1775 0.0 41.323944 1 ACGGCTG 1210 0.0 40.537193 8 AATCTGT 1805 0.0 40.512463 2 CGATGAA 435 0.0 40.344826 19 TACCTGT 1585 0.0 39.889587 7 TCGTTCA 85 0.0 39.705883 16 CGACCAC 995 0.0 39.346733 12 ATGATAC 4475 0.0 39.318436 3 GAATGAT 4705 0.0 39.117958 1 AATGATA 4420 0.0 38.789593 2 GATGAAT 470 0.0 38.776596 20 >>END_MODULE