##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933314.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	600529
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.474435039773265	31.0	28.0	33.0	25.0	34.0
2	28.821986948174025	31.0	28.0	33.0	16.0	34.0
3	28.81572913214849	31.0	28.0	34.0	16.0	34.0
4	33.17454943891136	35.0	32.0	37.0	28.0	37.0
5	34.22420232828057	35.0	33.0	37.0	30.0	37.0
6	33.7682493268435	35.0	33.0	37.0	28.0	37.0
7	33.58031502225538	35.0	32.0	37.0	28.0	37.0
8	33.6291985899099	35.0	33.0	37.0	28.0	37.0
9	34.72532550468004	37.0	34.0	39.0	28.0	39.0
10	34.494773774455524	37.0	32.0	39.0	27.0	39.0
11	34.64935914835087	37.0	33.0	39.0	27.0	39.0
12	34.74509141107257	37.0	33.0	39.0	27.0	39.0
13	34.835250254359075	37.0	34.0	39.0	27.0	39.0
14	35.70998236554771	38.0	34.0	40.0	27.0	41.0
15	36.04107212141295	38.0	34.0	40.0	27.0	41.0
16	35.89713902242856	38.0	34.0	40.0	27.0	41.0
17	35.27295101485524	37.0	33.0	40.0	27.0	41.0
18	35.00941503241309	37.0	33.0	39.0	27.0	40.0
19	34.27267459190147	37.0	33.0	37.0	27.0	40.0
20	33.67625376959314	35.0	32.0	37.0	27.0	39.0
21	33.945781136298166	35.0	33.0	38.0	27.0	40.0
22	34.46141318737313	35.0	33.0	38.0	28.0	40.0
23	34.885202879461275	35.0	33.0	39.0	29.0	40.0
24	34.32158979832781	35.0	33.0	38.0	27.0	40.0
25	33.411422262705045	35.0	33.0	38.0	24.0	40.0
26	33.36942595611536	35.0	32.0	38.0	24.0	40.0
27	34.09291974242709	35.0	33.0	38.0	25.0	40.0
28	33.86999795180582	35.0	33.0	38.0	24.0	40.0
29	33.951579357533106	35.0	33.0	39.0	24.0	40.0
30	33.18382792504608	35.0	33.0	38.0	21.0	40.0
31	32.99907748002178	35.0	32.0	38.0	21.0	40.0
32	32.401088040710775	35.0	31.0	38.0	18.0	40.0
33	31.806475623991513	35.0	31.0	39.0	13.0	40.0
34	31.05832690844239	35.0	29.0	39.0	10.0	40.0
35	30.51782345232287	35.0	25.0	39.0	8.0	40.0
36	30.01391606400357	35.0	23.0	39.0	7.0	40.0
37	29.899891595576566	35.0	23.0	39.0	7.0	40.0
38	29.64235032779433	35.0	22.0	39.0	7.0	40.0
39	29.23341587167314	35.0	21.0	38.0	7.0	40.0
40	28.921324365684256	35.0	21.0	38.0	7.0	40.0
41	28.476931172349712	34.0	18.0	38.0	7.0	40.0
42	28.646997896854273	34.0	18.0	38.0	7.0	40.0
43	28.635123366232104	34.0	18.0	38.0	7.0	40.0
44	28.672633628017966	35.0	18.0	38.0	7.0	40.0
45	28.79638951657622	35.0	20.0	39.0	7.0	40.0
46	28.680205285673132	35.0	20.0	38.0	7.0	40.0
47	28.61423511603936	34.0	20.0	38.0	7.0	40.0
48	28.466651901906484	34.0	20.0	38.0	7.0	40.0
49	28.620544553218913	34.0	20.0	38.0	7.0	40.0
50	28.62600307395646	34.0	20.0	38.0	7.0	40.0
51	27.5494172637791	33.0	18.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	12.0
9	35.0
10	45.0
11	47.0
12	64.0
13	80.0
14	115.0
15	208.0
16	365.0
17	701.0
18	1396.0
19	2389.0
20	3914.0
21	5641.0
22	8138.0
23	11692.0
24	17404.0
25	24725.0
26	31514.0
27	32965.0
28	31064.0
29	29044.0
30	29624.0
31	32661.0
32	37160.0
33	42111.0
34	45944.0
35	53115.0
36	56849.0
37	55634.0
38	40350.0
39	5518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.705051712739934	2.500628612440032	31.774485495288317	41.01983417953171
2	40.01155647770549	13.978342428092565	31.717202666315863	14.29289842788608
3	12.238876057609207	14.247771548085106	58.82480279886567	14.688549595440021
4	10.933193900710874	2.9014418953955596	69.37916403704068	16.78620016685289
5	19.090168834477602	3.2428075913069976	59.979284930452984	17.687738643762415
6	13.920393519713453	12.006414344686101	62.94067397244763	11.13251816315282
7	52.04827743539446	1.3125094708165634	43.96340559739829	2.675807496390682
8	51.22816716594869	8.490514196649954	37.11494365800819	3.166374979393168
9	48.19717282595845	2.5291035070745957	36.290337352567484	12.98338631439947
10	30.39769936172941	14.883377821887036	42.254911919324464	12.464010897059092
11	25.00711872365864	14.288735431594477	46.90214793956661	13.801997905180267
12	19.702795368749886	13.070476196819806	50.79454947221534	16.43217896221498
13	18.34366033946737	13.52374323304953	55.99213360220738	12.140462825275716
14	15.132991079531546	17.898552775969186	51.34190022463528	15.626555919863986
15	13.302438350187916	13.299440992857964	58.13874101000951	15.259379646944609
16	16.55074109659983	15.671016720258304	48.624462765328566	19.153779417813293
17	16.556569291408074	15.92562557345274	51.81065360706977	15.707151528069419
18	17.59282232831387	14.841414819267678	50.713454304454906	16.852308547963545
19	16.51910232478365	17.512892799515097	47.52976126048867	18.43824361521259
20	18.178805686319894	18.46338811281387	51.20668610508402	12.151120095782218
21	17.527546546461537	20.32324833605038	49.5714611617424	12.577743955745685
22	15.72530219190081	13.451140577723974	52.22562107741674	18.597936152958475
23	16.2000502889952	18.801090371988696	51.671776050781894	13.327083288234206
24	18.074397739326496	16.288305810377185	48.61813501096533	17.01916143933099
25	16.40836662342701	22.06837638149032	45.91951429489667	15.603742700186002
26	15.567940932078217	14.390978620516245	49.92681452519362	20.114265922211917
27	17.477091031407312	15.256382289614656	48.67874823697107	18.587778442006964
28	12.81103826792711	17.649272558028006	50.48215823049345	19.057530943551434
29	17.41314740836829	15.7865814973132	47.147598200919525	19.652672893398986
30	16.984525310184853	17.385338593140382	51.63347648489914	13.996659611775616
31	18.750468337082804	14.935165495754577	49.04375975181881	17.270606415343806
32	20.608163802247685	17.505399406190207	46.95993032809406	14.926506463468042
33	21.750156944960196	16.109630009541586	41.84044400853248	20.299769036965742
34	21.17799473464229	19.000414634430644	42.703682919559256	17.11790771136781
35	17.159704194135504	24.07943663003785	41.635291551282286	17.12556762454436
36	19.73326850160442	21.926168428169166	37.581199242667715	20.7593638275587
37	18.95262343700304	22.622054888273503	41.688244864111475	16.737076810611978
38	23.065330733403382	22.45969803290099	36.42222107508547	18.05275015861016
39	24.655595316795694	20.366043937928062	36.11415934950685	18.8642013957694
40	23.601025096206843	23.11911664549089	36.32813735889524	16.95172089940702
41	21.276574486827446	23.006715745617615	33.75174221394804	21.964967553606904
42	19.256522166290054	23.467476175172223	38.96198185266657	18.31401980587116
43	21.799280301201108	19.553760101510502	35.13835301875513	23.508606578533257
44	23.065830292958374	20.65212504308701	34.94369131215978	21.338353351794833
45	19.700630610678253	20.13091790737833	35.440919589228834	24.72753189271459
46	22.360618721160844	21.938157857488978	34.42248417645109	21.278739244899082
47	17.405320975340075	20.896576185329934	42.006131260938275	19.69197157839172
48	19.866484382935713	23.226688469665913	34.807977633053525	22.098849514344852
49	19.12130804673879	21.149519840007727	39.12050875145081	20.608663361802677
50	19.025559132031926	20.065808645377658	37.31826439689008	23.59036782570034
51	19.04454239512163	20.268296785001226	35.39079711387793	25.29636370599921
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	60.0
1	1110.5
2	2161.0
3	23789.5
4	45418.0
5	32785.0
6	20152.0
7	18874.5
8	17597.0
9	16934.0
10	16271.0
11	15400.0
12	14529.0
13	13522.5
14	12516.0
15	11593.5
16	10671.0
17	9918.5
18	9166.0
19	8448.5
20	7731.0
21	7221.0
22	6711.0
23	6681.5
24	6652.0
25	6555.5
26	6722.5
27	6986.0
28	7281.0
29	7576.0
30	8012.0
31	8448.0
32	8836.5
33	9225.0
34	10067.5
35	10910.0
36	11281.0
37	11652.0
38	12175.5
39	12699.0
40	13331.5
41	13964.0
42	14556.0
43	15148.0
44	17049.0
45	18950.0
46	45874.0
47	72798.0
48	52156.0
49	31514.0
50	30485.5
51	29457.0
52	27796.5
53	26136.0
54	26774.5
55	27413.0
56	27194.5
57	26976.0
58	25843.0
59	24710.0
60	22144.0
61	19578.0
62	17617.0
63	15656.0
64	13611.5
65	11567.0
66	9780.0
67	7993.0
68	6713.5
69	5434.0
70	4460.5
71	3487.0
72	2883.5
73	2280.0
74	1994.0
75	1425.0
76	1142.0
77	863.5
78	585.0
79	415.0
80	245.0
81	176.0
82	107.0
83	78.5
84	50.0
85	36.0
86	22.0
87	15.5
88	9.0
89	6.5
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	600529.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.0591554471152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.29668515908128	41.281184591375656
2	9.000507725785706	9.371176615992788
3	4.609986352550338	7.199759884094932
4	2.7293111438773994	5.6834253241062935
5	1.5858684354542993	4.127948569999438
6	0.944664081835396	2.950704856894514
7	0.5453080753393131	1.9871794502459559
8	0.31478785162886014	1.3110071760648212
9	0.19725066750369086	0.924183284545969
>10	0.6774223164737074	6.09971221636834
>50	0.04764555876449863	1.7506136447832825
>100	0.0447284818932976	3.935041882296357
>500	0.0022688360380658077	0.736691945375663
>1k	0.0022688360380658077	2.6621137934475114
>5k	3.2411943400940105E-4	1.5143862138677453
>10k+	9.723583020282032E-4	8.464870550540743
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22537	3.7528578969541857	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC	15051	2.506290287396612	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCG	12579	2.094653214082917	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGC	8975	1.4945156686854424	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT	3396	0.5655014162513383	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTC	2685	0.44710580171815184	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	2199	0.36617715380939136	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTGGACT	2151	0.3581842009295138	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCT	2012	0.33503794154820166	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTA	1766	0.29407405803882913	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTT	1568	0.2611031274093341	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGT	812	0.1352141195512623	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	720	0.11989429319816361	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	645	0.1074053043233549	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	601	0.10007843085013379	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6651985166411614E-4	0.0	0.0	0.04396124083932666	0.0
2	1.6651985166411614E-4	0.0	0.0	0.4695859816928075	0.0
3	1.6651985166411614E-4	0.0	0.0	0.5668335750646514	0.0
4	1.6651985166411614E-4	0.0	0.0	1.2500645264425199	0.0
5	1.6651985166411614E-4	0.0	0.0	4.236265026335115	0.0
6	1.6651985166411614E-4	0.0	0.0	4.666219283331863	0.0
7	1.6651985166411614E-4	0.0	0.0	5.194753292513767	0.0
8	1.6651985166411614E-4	0.0	0.0	5.86482917561017	0.0
9	1.6651985166411614E-4	0.0	0.0	6.052996607990622	0.0
10	1.6651985166411614E-4	0.0	0.0	8.760109836494157	0.0
11	1.6651985166411614E-4	0.0	0.0	8.989574192087309	0.0
12	1.6651985166411614E-4	0.0	0.0	10.907216803851272	0.0
13	1.6651985166411614E-4	0.0	0.0	11.083727846615234	0.0
14	1.6651985166411614E-4	0.0	0.0	11.221273244089794	0.0
15	1.6651985166411614E-4	0.0	0.0	11.63291031740349	0.0
16	1.6651985166411614E-4	0.0	0.0	11.847054846643543	0.0
17	1.6651985166411614E-4	0.0	0.0	11.969446937616668	0.0
18	1.6651985166411614E-4	0.0	0.0	12.059700697218618	0.0
19	1.6651985166411614E-4	0.0	0.0	12.58806818654886	0.0
20	1.6651985166411614E-4	0.0	0.0	12.70579772167539	0.0
21	1.6651985166411614E-4	0.0	0.0	12.791055885727417	0.0
22	1.6651985166411614E-4	0.0	0.0	12.973728163002953	0.0
23	1.6651985166411614E-4	0.0	0.0	13.067312319638186	0.0
24	1.6651985166411614E-4	0.0	0.0	13.160396916718426	0.0
25	1.6651985166411614E-4	0.0	0.0	13.235663889670606	0.0
26	1.6651985166411614E-4	0.0	0.0	13.331079764674145	0.0
27	1.6651985166411614E-4	0.0	0.0	13.48660930612843	0.0
28	1.6651985166411614E-4	0.0	0.0	13.57153443047713	0.0
29	1.6651985166411614E-4	0.0	0.0	13.676941496580515	0.0
30	1.6651985166411614E-4	0.0	0.0	13.801664865476939	0.0
31	1.6651985166411614E-4	0.0	0.0	13.901576776475407	0.0
32	1.6651985166411614E-4	0.0	0.0	14.01697503367864	0.0
33	1.6651985166411614E-4	0.0	0.0	14.124713377705323	0.0
34	1.6651985166411614E-4	0.0	0.0	14.295229705809378	0.0
35	1.6651985166411614E-4	0.0	0.0	14.450093167857005	0.0
36	1.6651985166411614E-4	0.0	0.0	14.5833090491883	0.0
37	1.6651985166411614E-4	0.0	0.0	14.722020085624507	0.0
38	1.6651985166411614E-4	0.0	0.0	14.911686196669937	0.0
39	1.6651985166411614E-4	0.0	0.0	15.288354101134166	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCA	25	3.888572E-5	45.000004	10
CGACGTC	20	7.030592E-4	45.0	45
AGGGATA	20	7.030592E-4	45.0	6
CGTTTTT	16795	0.0	44.169395	1
GATACCT	1465	0.0	42.84983	5
TACGGCT	1155	0.0	42.272728	7
TGATACC	1505	0.0	42.159466	4
CCGATGA	425	0.0	41.823532	18
ATACCTG	1520	0.0	41.595394	6
GAATCTG	1775	0.0	41.323944	1
ACGGCTG	1210	0.0	40.537193	8
AATCTGT	1805	0.0	40.512463	2
CGATGAA	435	0.0	40.344826	19
TACCTGT	1585	0.0	39.889587	7
TCGTTCA	85	0.0	39.705883	16
CGACCAC	995	0.0	39.346733	12
ATGATAC	4475	0.0	39.318436	3
GAATGAT	4705	0.0	39.117958	1
AATGATA	4420	0.0	38.789593	2
GATGAAT	470	0.0	38.776596	20
>>END_MODULE