FastQCFastQC Report
Sat 14 Jan 2017
SRR2933312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825433
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC239862.905868798557848No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG208882.530550632213638No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186562.2601470985531233No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC180512.18685223391844No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT61010.7391272217127254TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTC44260.5362034229307527No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC41070.49755703975973825TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCCCCTAT40680.49283224683287435No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTA36970.44788613975937475No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT35470.42971385927143696No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT19160.23212059609925945No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGT14950.18111706219644721No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTAT8980.10879138585445458No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATG8930.10818564317152332No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC207.03213E-445.023
CGTTTTT148100.043.9669151
TACGGCT22900.042.6419227
ATACCTG27450.041.8852466
GATACCT27400.041.879565
TGATACC27300.041.8681344
ACGGCTG23400.041.730778
CGATGAA6950.041.1151119
CACGACC4300.040.81395327
CCGATGA6850.040.72992718
AATGATC6250.040.682
GAATCTG29050.040.4302941
ACGACCA4300.040.290728
TACCTGT28400.040.2464757
GCGAGAC4250.040.23529421
GGCGACT5450.040.0458710
ATGATCT6300.039.9999963
GAATGAT89250.039.9327741
ACACGAC4350.039.82758726
CGAGACA4300.039.7674422
Produced by FastQC (version 0.11.5)