##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933312.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	825433
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.341140952687862	31.0	28.0	31.0	25.0	34.0
2	28.520806655416006	31.0	27.0	33.0	16.0	34.0
3	28.58646673927502	31.0	27.0	34.0	16.0	34.0
4	32.9596248272119	35.0	32.0	37.0	28.0	37.0
5	34.10454876410321	35.0	33.0	37.0	30.0	37.0
6	33.54273454053812	35.0	33.0	37.0	28.0	37.0
7	33.25173454417257	35.0	32.0	36.0	28.0	37.0
8	33.34794586598791	35.0	33.0	37.0	28.0	37.0
9	34.41341332367376	37.0	33.0	39.0	27.0	39.0
10	34.14944641176207	37.0	32.0	39.0	27.0	39.0
11	34.42181618617138	37.0	32.0	39.0	27.0	39.0
12	34.561097024228495	37.0	33.0	39.0	27.0	39.0
13	34.802303760571725	37.0	34.0	39.0	27.0	39.0
14	35.59563162606777	38.0	34.0	40.0	27.0	41.0
15	35.826596465128	38.0	34.0	40.0	27.0	41.0
16	35.65266835709258	37.0	34.0	40.0	27.0	41.0
17	35.06861126221026	37.0	33.0	40.0	27.0	41.0
18	34.832888920118286	37.0	33.0	39.0	27.0	40.0
19	34.160163211308486	37.0	32.0	38.0	26.0	40.0
20	33.904840247482234	35.0	32.0	38.0	27.0	40.0
21	34.41356354785913	35.0	33.0	39.0	27.0	40.0
22	35.06763843946147	36.0	33.0	39.0	29.0	40.0
23	35.40613350811029	36.0	34.0	39.0	30.0	40.0
24	34.93678953955076	36.0	33.0	39.0	28.0	40.0
25	33.88374586429183	35.0	33.0	39.0	24.0	40.0
26	34.032492037512434	35.0	33.0	39.0	25.0	40.0
27	34.64988072926573	36.0	33.0	39.0	27.0	40.0
28	34.387647452912596	36.0	33.0	39.0	25.0	40.0
29	34.65331165582185	36.0	33.0	39.0	25.0	40.0
30	33.961570472709475	35.0	33.0	39.0	23.0	40.0
31	33.93289946004097	35.0	33.0	39.0	24.0	40.0
32	33.574196815489564	36.0	33.0	39.0	21.0	40.0
33	33.284083626411835	36.0	33.0	39.0	18.0	40.0
34	33.06579698170536	37.0	33.0	40.0	15.0	41.0
35	32.715605021849136	37.0	32.0	40.0	12.0	41.0
36	32.382479256341824	37.0	31.0	40.0	10.0	41.0
37	32.38600346727112	37.0	31.0	40.0	10.0	41.0
38	32.095156118061674	36.0	31.0	40.0	10.0	41.0
39	31.720116593351612	36.0	30.0	39.0	10.0	40.0
40	31.483494117632805	35.0	30.0	39.0	9.0	40.0
41	30.91099580462618	35.0	27.0	39.0	8.0	40.0
42	31.242315245452993	35.0	29.0	39.0	8.0	40.0
43	31.11292981986424	35.0	29.0	39.0	8.0	40.0
44	31.307831162553473	36.0	29.0	39.0	8.0	40.0
45	31.38723312491747	36.0	30.0	39.0	8.0	40.0
46	31.16030374361093	35.0	29.0	39.0	8.0	40.0
47	31.101486129098305	35.0	29.0	39.0	8.0	40.0
48	30.94269189625324	35.0	29.0	39.0	8.0	40.0
49	31.14820463926206	35.0	29.0	39.0	8.0	40.0
50	31.106940236215415	35.0	29.0	39.0	8.0	40.0
51	29.67046023117564	34.0	24.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	66.0
10	53.0
11	57.0
12	78.0
13	106.0
14	142.0
15	199.0
16	363.0
17	673.0
18	1341.0
19	2390.0
20	3893.0
21	5785.0
22	8675.0
23	11895.0
24	17031.0
25	24012.0
26	30388.0
27	33392.0
28	32693.0
29	32947.0
30	35976.0
31	42203.0
32	50549.0
33	61552.0
34	71474.0
35	84797.0
36	93820.0
37	96658.0
38	72145.0
39	10061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.78981940387651	2.679321035141556	35.409415421966415	32.12144413901552
2	30.867799082420984	17.15293670110112	35.70283717757831	16.276427038899584
3	13.895858294979726	17.364704343053887	52.96529215575341	15.77414520621298
4	12.63143101862901	3.228123905877279	65.30402830999003	18.83641676550368
5	22.482139676993775	3.8169057936864657	53.528027108196554	20.172927421123216
6	15.496351611820705	14.74135393181518	57.19289148846727	12.56940296789685
7	59.54389998946008	1.5169008265964652	35.83198151757926	3.1072176663641993
8	58.30963869871933	10.75811119739579	27.15071968288159	3.78153042100328
9	54.89833820552364	3.0517316366076956	26.121926310191135	15.928003847677521
10	33.54457599829423	18.060823834278494	33.52022514244039	14.874375024986886
11	26.442969932144706	17.14978683914988	39.562266107606554	16.844977121098868
12	19.5870530981921	15.823937254749932	44.32994561642193	20.259064030636043
13	18.749916710381097	17.04462990939301	49.83214870255975	14.373304677666146
14	16.912820301587168	22.54550036162838	42.458321874700914	18.083357462083537
15	14.01821831693184	16.388610583778455	51.059867972324824	18.533303126964878
16	16.339060832314676	19.070718035261493	40.83808134639638	23.752139786027453
17	16.77992035695205	19.32234354575114	44.70102358398562	19.196712513311194
18	17.50329826890856	17.98922504915602	43.419635512512826	21.087841169422596
19	16.902280378904162	20.106053428927606	40.04782944224425	22.943836749923978
20	19.707595892095423	20.2206599445382	44.99735290447559	15.074391258890788
21	19.475475295996162	23.327877610902398	41.28305992127768	15.913587171823758
22	17.84760240988669	16.0577539303614	43.6807106088562	22.41393305089571
23	18.28010268549961	22.37165221162711	43.0118495383635	16.33639556450978
24	19.61576530136304	18.722173695502846	40.95389934737283	20.708161655761277
25	16.808996005732748	26.457871202144812	38.32267428125602	18.410458510866416
26	17.24888634207743	17.01397932963669	42.93358758372878	22.8035467445571
27	19.80330323599856	19.07217181770053	40.05219078956136	21.072334156739554
28	14.277718482299592	20.31915370478282	44.217519774469885	21.1856080384477
29	18.747130294039614	17.374517374517374	43.119429438852094	20.75892289259092
30	16.69354145036605	20.6647904796634	44.839375212767116	17.80229285720343
31	19.7703508340471	17.506569279396388	37.706391675641754	25.016688210914754
32	20.1783791052696	20.74571770210302	38.02052983100991	21.055373361617477
33	17.473859174518104	18.71647971428329	34.738252529278576	29.071408581920032
34	19.156248902091388	19.902160441852942	38.57744965369691	22.36414100235876
35	17.01361588402693	22.252199754553065	39.88403662077964	20.850147740640367
36	18.740951718673713	25.405332716283453	33.55341984146503	22.300295723577808
37	18.148656523303526	22.181085563576936	37.337857827346376	22.332400085773163
38	19.58063222575303	23.051295501875984	31.235000296813915	26.13307197555707
39	21.14284260503275	22.21646093626012	31.533389142425854	25.107307316281275
40	19.91427529551157	21.407188711863956	36.96581067149	21.71272532113448
41	18.706182088673458	26.9330157626361	30.479275725588874	23.881526423101572
42	19.175753816481773	22.257772587236033	38.334789134914644	20.23168446136755
43	21.726536254305316	23.02888302260753	31.86824369755026	23.376337025536902
44	22.018746524551357	21.54832675698694	32.90309449707002	23.52983222139168
45	19.60449848746052	20.555514499662603	33.01527804194889	26.824708970927986
46	23.916417201638414	23.31006877602422	31.121726415105766	21.651787607231597
47	16.73303587329317	21.868764636257577	40.09592541126899	21.30227407918026
48	20.189040176489186	23.8592350923697	31.40812155559567	24.543603175545442
49	18.759729741844584	20.9388284694215	38.26125197320679	22.04018981552712
50	20.003804064048808	21.681832444304987	34.85722039220627	23.457143099439932
51	19.378798763800333	20.936526647226366	32.879228235362525	26.805446353610773
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83.0
1	995.0
2	1907.0
3	20198.5
4	38490.0
5	28174.5
6	17859.0
7	16892.5
8	15926.0
9	15565.0
10	15204.0
11	14526.0
12	13848.0
13	13020.0
14	12192.0
15	11475.5
16	10759.0
17	9909.5
18	9060.0
19	8489.5
20	7919.0
21	7620.0
22	7321.0
23	7116.0
24	6911.0
25	7197.5
26	8209.5
27	8935.0
28	9511.5
29	10088.0
30	11219.0
31	12350.0
32	13218.5
33	14087.0
34	16177.0
35	18267.0
36	19028.5
37	19790.0
38	20882.5
39	21975.0
40	23015.0
41	24055.0
42	25270.5
43	26486.0
44	30229.5
45	33973.0
46	79419.5
47	124866.0
48	89108.5
49	53351.0
50	50010.5
51	46670.0
52	43296.0
53	39922.0
54	39449.0
55	38976.0
56	37893.5
57	36811.0
58	34934.0
59	33057.0
60	29832.0
61	26607.0
62	23949.5
63	21292.0
64	18539.5
65	15787.0
66	13466.0
67	11145.0
68	9471.0
69	7797.0
70	6415.0
71	5033.0
72	4342.0
73	3651.0
74	3123.5
75	2096.0
76	1596.0
77	1165.0
78	734.0
79	544.5
80	355.0
81	241.5
82	128.0
83	91.5
84	55.0
85	36.0
86	17.0
87	14.0
88	11.0
89	7.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	825433.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.43916741969048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4629341671797	33.343170000332925
2	5.859205837669672	4.856012233072427
3	2.733137713399884	3.397768538599434
4	1.8838193601648414	3.122556234173002
5	1.4782682635206232	3.0629103031623113
6	1.3124602298059678	3.263235551676896
7	1.071432330391656	3.107948460257827
8	0.9136460875558179	3.0288586547656577
9	0.7783142663059816	2.9027425667928415
>10	3.3922453268853467	20.604155029667165
>50	0.06854384274657614	1.9395868414074973
>100	0.040354774934554985	3.160587039174514
>500	0.0020770840039804326	0.5694319397137219
>1k	0.0020770840039804326	2.8595787497262615
>5k	2.9672628628291895E-4	0.750184466463705
>10k+	0.0011869051451316758	10.031273391013851
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	23986	2.905868798557848	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	20888	2.530550632213638	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18656	2.2601470985531233	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	18051	2.18685223391844	No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	6101	0.7391272217127254	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTC	4426	0.5362034229307527	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	4107	0.49755703975973825	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCCCCTAT	4068	0.49283224683287435	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTA	3697	0.44788613975937475	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	3547	0.42971385927143696	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	1916	0.23212059609925945	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGT	1495	0.18111706219644721	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTAT	898	0.10879138585445458	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATG	893	0.10818564317152332	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07232567634199262	0.0
2	0.0	0.0	0.0	0.6653477629316976	0.0
3	0.0	0.0	0.0	0.7987323017131615	0.0
4	0.0	0.0	0.0	1.7039541670856386	0.0
5	0.0	0.0	0.0	5.206479508330779	0.0
6	0.0	0.0	0.0	5.60081799491903	0.0
7	0.0	0.0	0.0	6.202562776142946	0.0
8	0.0	0.0	0.0	6.960225723953368	0.0
9	0.0	0.0	0.0	7.105361670783698	0.0
10	0.0	0.0	0.0	10.178900043976919	0.0
11	0.0	0.0	0.0	10.407265035442004	0.0
12	0.0	0.0	0.0	13.129714949608266	0.0
13	0.0	0.0	0.0	13.36716608131732	0.0
14	0.0	0.0	0.0	13.546344766928389	0.0
15	0.0	0.0	0.0	14.144333943518129	0.0
16	0.0	0.0	0.0	14.432182866447064	0.0
17	0.0	0.0	0.0	14.573684357179808	0.0
18	0.0	0.0	0.0	14.681385406204985	0.0
19	0.0	0.0	0.0	15.38210854181987	0.0
20	0.0	0.0	0.0	15.534149955235616	0.0
21	0.0	0.0	0.0	15.655298491821869	0.0
22	0.0	0.0	0.0	15.92776155060435	0.0
23	0.0	0.0	0.0	16.07035337816637	0.0
24	0.0	0.0	0.0	16.197195895972175	0.0
25	0.0	0.0	0.0	16.308410252558353	0.0
26	0.0	0.0	0.0	16.447125326949614	0.0
27	0.0	0.0	0.0	16.640478391341272	0.0
28	0.0	0.0	0.0	16.75593294670797	0.0
29	0.0	0.0	0.0	16.891861604757747	0.0
30	0.0	0.0	0.0	17.06110611036874	0.0
31	0.0	0.0	0.0	17.19618672866241	0.0
32	0.0	0.0	0.0	17.352226043785503	0.0
33	0.0	0.0	0.0	17.506811576469563	0.0
34	0.0	0.0	0.0	17.698953155495357	0.0
35	0.0	0.0	0.0	17.8889140608626	0.0
36	0.0	0.0	0.0	18.0528280308638	0.0
37	0.0	0.0	0.0	18.228008814767524	0.0
38	0.0	0.0	0.0	18.424511741110422	0.0
39	0.0	0.0	0.0	18.70775701964908	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	20	7.03213E-4	45.0	23
CGTTTTT	14810	0.0	43.966915	1
TACGGCT	2290	0.0	42.641922	7
ATACCTG	2745	0.0	41.885246	6
GATACCT	2740	0.0	41.87956	5
TGATACC	2730	0.0	41.868134	4
ACGGCTG	2340	0.0	41.73077	8
CGATGAA	695	0.0	41.11511	19
CACGACC	430	0.0	40.813953	27
CCGATGA	685	0.0	40.729927	18
AATGATC	625	0.0	40.68	2
GAATCTG	2905	0.0	40.430294	1
ACGACCA	430	0.0	40.2907	28
TACCTGT	2840	0.0	40.246475	7
GCGAGAC	425	0.0	40.235294	21
GGCGACT	545	0.0	40.04587	10
ATGATCT	630	0.0	39.999996	3
GAATGAT	8925	0.0	39.932774	1
ACACGAC	435	0.0	39.827587	26
CGAGACA	430	0.0	39.76744	22
>>END_MODULE