FastQCFastQC Report
Sat 14 Jan 2017
SRR2933311.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933311.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597818
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292854.898648083530439No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG98131.6414694773325662No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC82901.3867096674907748No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC75881.2692826244776838No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT29730.4973085454101416TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC23730.3969435513818587TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCCCCTAT20360.34057187973597314No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTC17650.29524035743319876No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTA12750.21327561231010106No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT11110.18584251394237042No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTTCCCCTATCGTATGCCG7910.13231451712728623No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT7900.1321472421372391No Hit
GAATCTTTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC6210.10387776881927274No Hit
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT6180.10337594384913133TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGG253.8885526E-545.02
CGTTTTT125550.044.4086041
TACGGCT11300.041.415937
TGATACC13500.041.3333324
GAATGAT39850.041.3299871
ACGGCTG11350.041.233488
GATACCT13550.041.180815
CGACCAC7600.040.85526312
GCGACCA7650.040.58823411
AATGATA38100.040.157482
ATGATAC38500.040.1493533
CCGATGA2100.039.64285718
ATACGGC25250.039.5643586
AATGATC3600.039.3752
CGTCACG403.4545155E-739.37537
TCACGTC403.4545155E-739.37539
CAAGACG1200.039.37499638
ATACCTG14300.039.3356676
ACACGAC2750.039.27272826
GTTTTTT144300.039.1216162
Produced by FastQC (version 0.11.5)