##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933311.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597818
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.369870094242728	31.0	27.0	31.0	16.0	33.0
2	28.689009029503964	31.0	30.0	33.0	16.0	34.0
3	28.94524420475797	31.0	30.0	33.0	16.0	34.0
4	31.792610794589656	35.0	28.0	35.0	19.0	37.0
5	33.43210642703967	35.0	33.0	37.0	28.0	37.0
6	32.64782759970426	35.0	33.0	37.0	17.0	37.0
7	31.553825746297367	33.0	30.0	35.0	23.0	37.0
8	32.7799815328411	35.0	32.0	35.0	27.0	37.0
9	32.45998949513063	34.0	31.0	37.0	25.0	39.0
10	30.650512363294514	34.0	27.0	37.0	15.0	39.0
11	31.549525106303257	35.0	27.0	39.0	15.0	39.0
12	32.47856371002546	35.0	30.0	39.0	21.0	39.0
13	32.76372909480812	35.0	31.0	39.0	22.0	39.0
14	33.02618188144218	36.0	31.0	39.0	18.0	41.0
15	33.50546989217454	36.0	31.0	39.0	22.0	41.0
16	33.596435704512075	36.0	31.0	39.0	23.0	41.0
17	31.529686292483664	34.0	27.0	38.0	17.0	41.0
18	32.38143548705459	35.0	29.0	39.0	19.0	39.0
19	32.1909343646394	35.0	30.0	37.0	18.0	39.0
20	32.21167646340525	34.0	30.0	37.0	23.0	39.0
21	32.71997664841139	35.0	31.0	37.0	23.0	39.0
22	33.29748853329943	35.0	31.0	38.0	25.0	39.0
23	34.31255666440288	35.0	33.0	39.0	27.0	40.0
24	32.5546571029979	35.0	31.0	38.0	22.0	39.0
25	31.667723287020465	35.0	30.0	37.0	18.0	39.0
26	31.329259072159086	34.0	27.0	37.0	20.0	39.0
27	32.75361397615997	35.0	31.0	38.0	22.0	40.0
28	32.680611825003595	35.0	31.0	38.0	22.0	40.0
29	33.194065752453085	35.0	31.0	38.0	22.0	40.0
30	32.10350641834137	35.0	30.0	38.0	19.0	40.0
31	32.15495184153036	35.0	30.0	38.0	21.0	40.0
32	32.246314095594315	35.0	30.0	38.0	20.0	40.0
33	32.428685653493204	35.0	31.0	38.0	18.0	40.0
34	31.911367673773622	35.0	31.0	38.0	15.0	40.0
35	31.463826783402308	35.0	30.0	38.0	13.0	40.0
36	30.979756380704494	35.0	29.0	38.0	10.0	40.0
37	31.245653359383624	35.0	30.0	38.0	10.0	40.0
38	31.08842992348842	35.0	29.0	38.0	10.0	40.0
39	30.635889183664595	35.0	28.0	38.0	10.0	40.0
40	30.35305895774299	35.0	26.0	38.0	10.0	40.0
41	29.697918095473874	34.0	25.0	38.0	8.0	40.0
42	30.286819734434225	35.0	26.0	38.0	9.0	40.0
43	30.2696874299536	35.0	26.0	38.0	8.0	40.0
44	30.415176525296996	35.0	27.0	38.0	8.0	40.0
45	30.672017236014973	35.0	28.0	38.0	9.0	40.0
46	30.427968043785903	35.0	27.0	38.0	8.0	40.0
47	30.508708335981854	35.0	28.0	38.0	8.0	40.0
48	30.279511490119067	34.0	27.0	38.0	8.0	40.0
49	30.103314052102814	34.0	27.0	38.0	8.0	40.0
50	30.250480915596384	34.0	27.0	38.0	8.0	40.0
51	28.427280543576806	33.0	23.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	22.0
10	32.0
11	56.0
12	59.0
13	100.0
14	185.0
15	263.0
16	517.0
17	942.0
18	1658.0
19	2786.0
20	4070.0
21	5900.0
22	8129.0
23	11436.0
24	15157.0
25	20816.0
26	26243.0
27	28843.0
28	30293.0
29	33458.0
30	38617.0
31	45039.0
32	52066.0
33	59475.0
34	65251.0
35	67250.0
36	49523.0
37	24649.0
38	4900.0
39	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.78981897500577	2.722902287987314	38.30061992111312	34.1866588158938
2	33.69185939533437	11.819316246750683	38.38910839084805	16.0997159670669
3	14.759341471819182	11.70841292834943	57.54928757581739	15.982958024013996
4	13.430508950884718	3.2575131561779673	64.05862653851172	19.253351354425593
5	19.449564917750887	3.815375247985173	56.91314078866812	19.821919045595816
6	15.978107049302631	10.228196541422307	59.763841169051446	14.029855240223613
7	59.86922441278115	1.580581380955408	35.116373210575794	3.433820995687651
8	60.75946190981202	7.265087367727302	27.663436029025558	4.312014693435126
9	56.32583829861262	2.91075210181025	27.952152661846917	12.811256937730212
10	32.03934976865869	18.219926465914373	34.679116386592575	15.06160737883436
11	24.159359537518107	17.546142806004504	41.7852925137751	16.509205142702292
12	19.518816763630404	15.863189131140246	45.97101458972463	18.646979515504718
13	17.797891666025446	17.156224804204623	50.23836686081717	14.807516668952758
14	16.575111488780866	18.905252100137503	45.70872740533072	18.810909005750915
15	14.054946488730685	16.64369423470019	51.13312078257931	18.16823849398981
16	16.1231344656735	17.241535049128665	43.52210873543453	23.113221749763305
17	16.633657735297362	17.84439411325855	45.939734166585815	19.582213984858267
18	17.01337196270437	16.709098755808625	45.36046087605258	20.91706840543443
19	16.90581414410406	19.498576489834697	41.90874145642988	21.68686790963136
20	18.835331154297798	19.604461558534535	45.7711209766183	15.789086310549363
21	19.532366037824218	20.977956501811587	42.3925007276462	17.097176732717983
22	18.016352803027008	16.932243592531506	43.92791786128889	21.1234857431526
23	18.091124723578076	21.026633523915304	43.09221201101339	17.79002974149323
24	18.31895326002228	18.155191044766134	42.952537394324025	20.57331830088756
25	16.999989963500596	22.19705662927513	40.668062855250255	20.134890551974014
26	17.025917586957902	17.825324764393176	43.63736120357701	21.511396445071913
27	17.69702484702702	18.74332990977187	42.470785422988264	21.08885982021284
28	14.784767270306348	19.1536554603575	45.230655483776	20.830921785560154
29	17.94843915706787	16.95415661622768	43.72936244810293	21.368041778601516
30	16.870184571224016	19.067341565493177	44.98743764824746	19.075036215035347
31	18.7065294119615	17.83084483906473	40.30390520191764	23.158720547056127
32	18.86861887731718	19.87009424272939	40.28266127818165	20.97862560177178
33	17.065059934628934	18.751191834304084	37.73874322954478	26.445005001522205
34	18.006149028634134	19.991368610513568	40.028570568300054	21.973911792552247
35	16.42891314747967	22.044334563362096	40.29989060215651	21.22686168700173
36	18.384859606100854	23.224292343154605	36.27675981653279	22.114088234211753
37	17.905951309595896	22.12111378379373	37.68973165746097	22.283203249149405
38	18.873804402008638	21.967889892910552	34.533587145251566	24.624718559829244
39	19.668862429702685	21.746250531098095	34.29655848435477	24.28832855484445
40	19.57284658541563	21.03633547333804	38.08634065886273	21.3044772823836
41	18.23464666503852	24.896707693645894	34.205226339789036	22.663419301526552
42	18.738980760030643	22.24389362648833	38.862496612681454	20.154629000799574
43	20.28108889327521	22.194045679454284	34.996604317702044	22.528261109568465
44	20.704294618094472	21.4418100492123	35.07656176294457	22.777333569748652
45	19.211699881903858	20.371417387900664	35.10131846147155	25.31556426872393
46	21.530465793937285	22.446965464405555	34.21258643935111	21.809982302306054
47	16.80160182530469	21.114285618700006	40.46047459260176	21.62363796339354
48	19.434008343676503	22.42488516571933	34.646330488543335	23.494776002060828
49	18.257897888655076	20.566460026295626	38.8129832156275	22.362658869421796
50	19.211699881903858	21.120140243351656	36.37177201087957	23.296387863864922
51	18.834996604317702	20.244622945444934	35.34286354709962	25.57751690313774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	115.0
1	554.0
2	993.0
3	21532.0
4	42071.0
5	27854.0
6	13637.0
7	12738.0
8	11839.0
9	11536.0
10	11233.0
11	10718.0
12	10203.0
13	9635.0
14	9067.0
15	8292.5
16	7518.0
17	6966.5
18	6415.0
19	5918.0
20	5421.0
21	5195.5
22	4970.0
23	4788.5
24	4607.0
25	4672.0
26	5276.5
27	5816.0
28	6280.0
29	6744.0
30	7580.0
31	8416.0
32	9108.5
33	9801.0
34	11114.5
35	12428.0
36	13132.0
37	13836.0
38	14761.5
39	15687.0
40	16409.5
41	17132.0
42	18422.5
43	19713.0
44	22226.0
45	24739.0
46	44503.5
47	64268.0
48	54509.5
49	44751.0
50	42092.0
51	39433.0
52	35572.5
53	31712.0
54	29900.0
55	28088.0
56	26688.0
57	25288.0
58	24118.0
59	22948.0
60	20794.0
61	18640.0
62	17144.5
63	15649.0
64	13865.5
65	12082.0
66	10472.0
67	8862.0
68	7713.5
69	6565.0
70	5437.0
71	4309.0
72	3668.5
73	3028.0
74	2685.0
75	1912.0
76	1482.0
77	1068.0
78	654.0
79	489.0
80	324.0
81	233.0
82	142.0
83	105.5
84	69.0
85	46.0
86	23.0
87	18.5
88	14.0
89	9.5
90	5.0
91	2.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	597818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.69905933105127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25394284839193	34.196236914205734
2	7.295838834767237	6.376425882205629
3	3.4785508129811604	4.5602819508762105
4	2.340676265004728	4.091414039169003
5	1.6901604633979557	3.6929211184512187
6	1.366190275728927	3.58207379499502
7	1.1049934600750608	3.3801022340570595
8	0.8683970801028988	3.0358508421062607
9	0.720385009202523	2.833213253250687
>10	2.784167102634358	17.501660843042206
>50	0.05363402893690628	1.5894775678812119
>100	0.036799990590658506	3.105220890474719
>500	0.0023489355696165006	0.6769521824477144
>1k	0.0023489355696165006	1.9730324791937048
>5k	0.0011744677848082503	4.39514241073142
>10k+	3.914892616027501E-4	5.0099935969121345
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29285	4.898648083530439	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCG	9813	1.6414694773325662	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	8290	1.3867096674907748	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGC	7588	1.2692826244776838	No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCT	2973	0.4973085454101416	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGC	2373	0.3969435513818587	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCCCCTAT	2036	0.34057187973597314	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTC	1765	0.29524035743319876	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTA	1275	0.21327561231010106	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCT	1111	0.18584251394237042	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTTCCCCTATCGTATGCCG	791	0.13231451712728623	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTT	790	0.1321472421372391	No Hit
GAATCTTTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTC	621	0.10387776881927274	No Hit
CTGTCTCTTATACACATCTGACGCTTCCCCTATCGTATGCCGTCTTCTGCT	618	0.10337594384913133	TruSeq Adapter, Index 16 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.130809042216862	0.0
2	0.0	0.0	0.0	0.7360099562074076	0.0
3	0.0	0.0	0.0	0.9477800936070845	0.0
4	0.0	0.0	0.0	1.8187809667825325	0.0
5	0.0	0.0	0.0	3.7825893499359338	0.0
6	0.0	0.0	0.0	4.351324316096203	0.0
7	0.0	0.0	0.0	4.91671378245553	0.0
8	0.0	0.0	0.0	5.684338711781846	0.0
9	0.0	0.0	0.0	5.993295618398911	0.0
10	0.0	0.0	0.0	8.407742824739302	0.0
11	0.0	0.0	0.0	9.10745410810648	0.0
12	0.0	0.0	0.0	11.110739388911007	0.0
13	0.0	0.0	0.0	11.40045967167265	0.0
14	0.0	0.0	0.0	11.577269336152474	0.0
15	0.0	0.0	0.0	12.021049884747532	0.0
16	0.0	0.0	0.0	12.303410067947102	0.0
17	0.0	0.0	0.0	12.569210027132003	0.0
18	0.0	0.0	0.0	12.814936987511249	0.0
19	0.0	0.0	0.0	13.425323426193255	0.0
20	0.0	0.0	0.0	13.668541261721796	0.0
21	0.0	0.0	0.0	13.93066117112566	0.0
22	0.0	0.0	0.0	14.304018948910873	0.0
23	0.0	0.0	0.0	14.592735581732233	0.0
24	0.0	0.0	0.0	14.83076789256931	0.0
25	0.0	0.0	0.0	15.045214429809741	0.0
26	0.0	0.0	0.0	15.250962667567721	0.0
27	0.0	0.0	0.0	15.462732804967398	0.0
28	0.0	0.0	0.0	15.655935418471843	0.0
29	0.0	0.0	0.0	15.874898380443547	0.0
30	0.0	0.0	0.0	16.12898909032515	0.0
31	0.0	0.0	0.0	16.32637357858077	0.0
32	0.0	0.0	0.0	16.538980090930686	0.0
33	0.0	0.0	0.0	16.753426628171116	0.0
34	0.0	0.0	0.0	16.99095711403805	0.0
35	0.0	0.0	0.0	17.25876437310352	0.0
36	0.0	0.0	0.0	17.468192660642536	0.0
37	0.0	0.0	0.0	17.686821072634146	0.0
38	0.0	0.0	0.0	17.929369808202498	0.0
39	0.0	0.0	0.0	18.26877076300814	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATGG	25	3.8885526E-5	45.0	2
CGTTTTT	12555	0.0	44.408604	1
TACGGCT	1130	0.0	41.41593	7
TGATACC	1350	0.0	41.333332	4
GAATGAT	3985	0.0	41.329987	1
ACGGCTG	1135	0.0	41.23348	8
GATACCT	1355	0.0	41.18081	5
CGACCAC	760	0.0	40.855263	12
GCGACCA	765	0.0	40.588234	11
AATGATA	3810	0.0	40.15748	2
ATGATAC	3850	0.0	40.149353	3
CCGATGA	210	0.0	39.642857	18
ATACGGC	2525	0.0	39.564358	6
AATGATC	360	0.0	39.375	2
CGTCACG	40	3.4545155E-7	39.375	37
TCACGTC	40	3.4545155E-7	39.375	39
CAAGACG	120	0.0	39.374996	38
ATACCTG	1430	0.0	39.335667	6
ACACGAC	275	0.0	39.272728	26
GTTTTTT	14430	0.0	39.121616	2
>>END_MODULE