##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933310.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752491
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.531490742081964	31.0	28.0	33.0	25.0	34.0
2	28.936029799691955	31.0	28.0	33.0	16.0	34.0
3	28.955623389515623	31.0	28.0	34.0	16.0	34.0
4	33.1642797056709	35.0	32.0	37.0	28.0	37.0
5	34.202308067471904	35.0	33.0	37.0	30.0	37.0
6	33.75169802695314	35.0	33.0	37.0	28.0	37.0
7	33.36780639236881	35.0	32.0	37.0	28.0	37.0
8	33.593835673782145	35.0	33.0	37.0	28.0	37.0
9	34.55487972613626	37.0	34.0	39.0	28.0	39.0
10	34.21811822334088	37.0	32.0	39.0	27.0	39.0
11	34.45432835741557	37.0	32.0	39.0	27.0	39.0
12	34.55895552239163	37.0	33.0	39.0	27.0	39.0
13	34.7809967162398	37.0	34.0	39.0	27.0	39.0
14	35.56482403111798	38.0	34.0	40.0	27.0	41.0
15	35.91600298209546	38.0	34.0	40.0	27.0	41.0
16	35.70107549459063	38.0	34.0	40.0	27.0	41.0
17	35.161635155769304	37.0	33.0	40.0	27.0	41.0
18	34.91090524670727	37.0	33.0	39.0	27.0	40.0
19	34.25591668205998	37.0	32.0	38.0	26.0	40.0
20	34.03124024074707	35.0	32.0	38.0	27.0	40.0
21	34.341468535836306	35.0	33.0	38.0	27.0	40.0
22	34.95537886831869	36.0	33.0	39.0	29.0	40.0
23	35.38163911594956	36.0	34.0	39.0	30.0	40.0
24	34.843687167022594	35.0	33.0	39.0	28.0	40.0
25	33.788137000974096	35.0	33.0	39.0	24.0	40.0
26	33.90215165364104	35.0	32.0	39.0	25.0	40.0
27	34.547534787791484	35.0	33.0	39.0	27.0	40.0
28	34.410661389970116	35.0	33.0	39.0	25.0	40.0
29	34.58528540540684	36.0	33.0	39.0	25.0	40.0
30	33.815114067809446	35.0	33.0	39.0	23.0	40.0
31	33.69103019172322	35.0	33.0	39.0	23.0	40.0
32	33.210575275983366	35.0	32.0	39.0	21.0	40.0
33	32.924986478243596	36.0	32.0	39.0	16.0	40.0
34	32.60332814611736	36.0	32.0	40.0	15.0	41.0
35	32.24983820404496	36.0	31.0	40.0	10.0	41.0
36	31.72297874658966	36.0	30.0	40.0	10.0	41.0
37	31.690777697009	36.0	30.0	40.0	8.0	41.0
38	31.361892700377812	35.0	29.0	39.0	8.0	41.0
39	31.035152579897964	35.0	29.0	39.0	8.0	40.0
40	30.79492246418894	35.0	27.0	39.0	8.0	40.0
41	30.379777299662056	35.0	25.0	39.0	8.0	40.0
42	30.583309302038163	35.0	26.0	39.0	7.0	40.0
43	30.479654906171636	35.0	26.0	39.0	7.0	40.0
44	30.60230354914544	35.0	27.0	39.0	7.0	40.0
45	30.683415482710092	35.0	27.0	39.0	7.0	40.0
46	30.49805645516026	35.0	26.0	39.0	7.0	40.0
47	30.452139626919127	35.0	26.0	39.0	7.0	40.0
48	30.283192755793756	35.0	26.0	38.0	7.0	40.0
49	30.42812339283792	35.0	27.0	39.0	7.0	40.0
50	30.414654793213472	35.0	26.0	39.0	7.0	40.0
51	29.04583975090732	34.0	23.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	23.0
9	50.0
10	50.0
11	60.0
12	73.0
13	101.0
14	157.0
15	185.0
16	384.0
17	675.0
18	1370.0
19	2357.0
20	3867.0
21	5821.0
22	8211.0
23	11796.0
24	17041.0
25	24486.0
26	30882.0
27	33357.0
28	32514.0
29	31719.0
30	32957.0
31	38095.0
32	45515.0
33	53798.0
34	61988.0
35	72669.0
36	82220.0
37	86143.0
38	64833.0
39	9093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.801637494667713	2.7894021323843075	37.332273741479966	34.076686631468014
2	33.54339121663914	13.56268712848393	36.93147160564047	15.962450049236468
3	14.350869312722677	13.869135976377125	55.92093460254009	15.8590601083601
4	12.68958698509351	3.3466180990868994	65.11147641632923	18.85231849949036
5	20.395194095344664	3.9377215142772473	56.37608954791485	19.29099484246323
6	16.06052431191868	12.30991467007579	59.135192314592466	12.494368703413064
7	60.14942371403777	1.4506485791856647	35.49411222194019	2.9058154848363635
8	59.838988107498956	8.342159574001549	28.662003930944024	3.1568483875554656
9	56.20239976292075	3.018507862552509	28.18492181301836	12.594170561508378
10	33.70551940156095	17.562070509813406	34.737292539046976	13.995117549578667
11	26.3268265002505	16.98345893837933	40.74972325250402	15.939991308866153
12	20.28675426018384	15.812813708070927	45.40811783795421	18.49231419379102
13	19.31451671847238	15.99819798509218	50.27834219944158	14.40894309699385
14	16.738804849493217	20.284096421086765	45.68174237299848	17.295356356421536
15	14.309008346943685	15.95939353427483	52.256040271577994	17.475557847203486
16	17.561007374174576	17.716623853308544	43.32490355366376	21.397465218853114
17	18.32048489616487	17.744265379918165	45.68652648337322	18.24872324054374
18	18.798231473864803	16.686179635371055	44.99788037332008	19.517708517444063
19	18.42626689222861	18.93019318503477	42.12581944501662	20.51772047772
20	19.664687019512524	19.721830560099722	45.950848581577716	14.662633838810033
21	19.660966044776615	21.64995993307561	43.83839806721941	14.850675954928363
22	18.11941936847085	15.343439323526791	46.036298108548806	20.50084319945355
23	18.230915718593312	20.809152534714702	45.078944465780985	15.880987280911002
24	19.26162572044051	18.71357929862284	42.719314915394335	19.305480065542312
25	17.86612730251923	24.0008186144419	40.45842408746417	17.674629995574698
26	17.35927738670629	17.140271445106986	44.3180051322873	21.18244603589943
27	19.28541338035937	18.295766992562037	41.952794119796785	20.466025507281817
28	15.296661355418204	19.536712066988176	44.94977348566295	20.216853091930666
29	18.65935938104243	18.356232832020584	43.62364466817543	19.360763118761554
30	18.521018856039472	19.846881889617286	42.99626174930996	18.63583750503328
31	22.38126436063687	17.98054727564848	40.43822451032637	19.19996385338828
32	21.35214906224792	22.50910642120637	39.39741471991027	16.74132979663544
33	19.479701418355834	18.407130450729646	39.482199787107085	22.630968343807435
34	20.34403069272589	20.697921968501948	42.20528883401928	16.752758504752883
35	20.392536256247585	22.886253789081863	35.401087853542435	21.32012210112812
36	22.514953667219938	22.209567954965575	34.547788611425254	20.727689766389233
37	19.692594330031852	25.064352929138025	34.754435601223136	20.488617139606983
38	23.51483273554102	22.824591922029633	34.13303282032609	19.527542522103257
39	22.01913378365987	23.70606425857585	34.49689099271619	19.777910965048086
40	23.814371201781814	21.829629856038146	35.79152441690333	18.564474525276715
41	20.358250131895264	23.597092855595612	33.53701240280615	22.50764460970297
42	21.98338584780416	22.428175220700314	36.05531494728841	19.53312398420712
43	21.925710739397548	20.93672881137449	33.54618194769107	23.591378501536894
44	22.75482364573131	21.740459354331147	33.25541435047064	22.249302649466905
45	20.999320922110694	21.07546801224201	32.70431141369133	25.220899651955968
46	23.091571859331207	23.49782256531972	31.706159940783348	21.70444563456573
47	17.90838694416279	22.118271181980916	39.68579026194333	20.287551611912967
48	20.44024446804015	24.53677186836786	32.71627168962818	22.306711973963807
49	20.085688732489825	22.267641739236748	36.488144044247704	21.158525484025724
50	19.695517953038642	21.615939592633	34.98314265552678	23.70539979880158
51	19.384949454544973	22.039599144707378	33.34086387744172	25.23458752330593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84.0
1	1011.5
2	1939.0
3	20186.5
4	38434.0
5	28429.0
6	18424.0
7	17523.0
8	16622.0
9	16169.0
10	15716.0
11	15057.0
12	14398.0
13	13366.5
14	12335.0
15	11549.0
16	10763.0
17	10021.0
18	9279.0
19	8652.0
20	8025.0
21	7713.5
22	7402.0
23	7247.0
24	7092.0
25	7149.5
26	7808.5
27	8410.0
28	8812.5
29	9215.0
30	10155.5
31	11096.0
32	12112.5
33	13129.0
34	14966.0
35	16803.0
36	17143.5
37	17484.0
38	18433.5
39	19383.0
40	20602.5
41	21822.0
42	22874.5
43	23927.0
44	26726.5
45	29526.0
46	60816.5
47	92107.0
48	68625.0
49	45143.0
50	43095.5
51	41048.0
52	38777.0
53	36506.0
54	37328.5
55	38151.0
56	36762.5
57	35374.0
58	33619.0
59	31864.0
60	28844.5
61	25825.0
62	23223.5
63	20622.0
64	17949.0
65	15276.0
66	12908.0
67	10540.0
68	9013.0
69	7486.0
70	6233.0
71	4980.0
72	4231.5
73	3483.0
74	2960.5
75	1996.0
76	1554.0
77	1220.5
78	887.0
79	645.0
80	403.0
81	295.0
82	187.0
83	125.0
84	63.0
85	41.0
86	19.0
87	13.5
88	8.0
89	5.5
90	3.0
91	3.5
92	4.0
93	4.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	752491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.75631557365955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.49143381901307	35.220085791610686
2	6.011978028345135	5.261240156603545
3	2.7460238307313056	3.6046765593080545
4	1.9855767223919665	3.4752608664278193
5	1.5338189363562593	3.355713270602966
6	1.2642650406342462	3.3191748052042542
7	1.060919260468341	3.249534257145837
8	0.8940256257867661	3.129541393029136
9	0.7065789267521595	2.782556144699865
>10	3.2077220552448487	20.53614263534338
>50	0.05083132967948482	1.541432164456403
>100	0.0409731215219339	3.002658000560177
>500	0.0021564800801007113	0.5551041816522095
>1k	0.0024645486629722418	2.7230839178135713
>5k	0.0	0.0
>10k+	0.0012322743314861209	8.243795855542139
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18725	2.488401854640122	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	16471	2.1888633883993296	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG	14212	1.8886604623842678	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	11748	1.5612146856241471	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	4306	0.5722327576010876	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTC	3005	0.39934032433610506	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGATTGCT	2811	0.3735592850944397	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	2791	0.3709014459973608	TruSeq Adapter, Index 27 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTA	2457	0.3265155330761431	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCT	2340	0.3109671743582315	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTT	1468	0.19508538972559142	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGT	1023	0.13594846981558584	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.328919548539451E-4	0.0	0.0	0.050498942844499135	0.0
2	1.328919548539451E-4	0.0	0.0	0.48904239386251797	0.0
3	1.328919548539451E-4	0.0	0.0	0.573561677149627	0.0
4	1.328919548539451E-4	0.0	0.0	1.266061653893535	0.0
5	1.328919548539451E-4	0.0	0.0	3.918585072778279	0.0
6	1.328919548539451E-4	0.0	0.0	4.2343363575112525	0.0
7	1.328919548539451E-4	0.0	0.0	4.684840084466127	0.0
8	1.328919548539451E-4	0.0	0.0	5.253484759286158	0.0
9	1.328919548539451E-4	0.0	0.0	5.377472953164888	0.0
10	1.328919548539451E-4	0.0	0.0	7.727667174756907	0.0
11	1.328919548539451E-4	0.0	0.0	7.939364058839242	0.0
12	1.328919548539451E-4	0.0	0.0	9.942843170217317	0.0
13	1.328919548539451E-4	0.0	0.0	10.145503401369584	0.0
14	1.328919548539451E-4	0.0	0.0	10.28650176546962	0.0
15	1.328919548539451E-4	0.0	0.0	10.748434200541933	0.0
16	1.328919548539451E-4	0.0	0.0	10.98445031236254	0.0
17	1.328919548539451E-4	0.0	0.0	11.11415286029999	0.0
18	1.328919548539451E-4	0.0	0.0	11.236546350720474	0.0
19	1.328919548539451E-4	0.0	0.0	11.804260781856527	0.0
20	1.328919548539451E-4	0.0	0.0	11.941671063175507	0.0
21	1.328919548539451E-4	0.0	0.0	12.064197445550844	0.0
22	1.328919548539451E-4	0.0	0.0	12.30154247691999	0.0
23	1.328919548539451E-4	0.0	0.0	12.433769971999665	0.0
24	1.328919548539451E-4	0.0	0.0	12.556296354375002	0.0
25	1.328919548539451E-4	0.0	0.0	12.658091591793124	0.0
26	1.328919548539451E-4	0.0	0.0	12.786066544317473	0.0
27	1.328919548539451E-4	0.0	0.0	12.953510407433445	0.0
28	1.328919548539451E-4	0.0	0.0	13.061418674774847	0.0
29	1.328919548539451E-4	0.0	0.0	13.194044845719086	0.0
30	1.328919548539451E-4	0.0	0.0	13.364412331841843	0.0
31	1.328919548539451E-4	0.0	0.0	13.500493693612283	0.0
32	1.328919548539451E-4	0.0	0.0	13.655844388836544	0.0
33	1.328919548539451E-4	0.0	0.0	13.808537244963727	0.0
34	1.328919548539451E-4	0.0	0.0	14.004552878373296	0.0
35	1.328919548539451E-4	0.0	0.0	14.206947325615854	0.0
36	1.328919548539451E-4	0.0	0.0	14.379972650835692	0.0
37	1.328919548539451E-4	0.0	0.0	14.563097764624427	0.0
38	1.328919548539451E-4	0.0	0.0	14.787419384417888	0.0
39	1.328919548539451E-4	0.0	0.0	15.204700122659274	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	50	2.1827873E-11	45.000004	14
GTTACGT	40	6.8102963E-9	45.0	22
CGTACAT	20	7.031732E-4	45.0	35
TTCGGTA	20	7.031732E-4	45.0	25
CGTTTTT	15405	0.0	44.006817	1
GATACCT	1665	0.0	42.162163	5
CGATGAA	390	0.0	42.115383	19
CCGATGA	385	0.0	42.07792	18
TGATACC	1685	0.0	41.928783	4
TAATCGT	65	0.0	41.538464	21
TACGGCT	1400	0.0	41.464287	7
TCAAGCG	290	0.0	41.12069	17
ATACCTG	1720	0.0	40.944763	6
CTACGAA	55	6.002665E-11	40.909092	11
GCTACGA	55	6.002665E-11	40.909092	10
ACGGCTG	1445	0.0	40.17301	8
CACGACC	315	0.0	40.0	27
GGCGACT	445	0.0	39.94382	10
GAATCTG	2000	0.0	39.9375	1
ATGATAC	5660	0.0	39.75265	3
>>END_MODULE