FastQCFastQC Report
Sat 14 Jan 2017
SRR2933308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821991
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297233.6159763306410895No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC158621.929704826451871No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG144111.753182212457314No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC119261.4508674669187376No Hit
GAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT36580.44501703789944175RNA PCR Primer, Index 40 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTC27490.3344318855072622No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAGAAGCT27080.32944399634545873No Hit
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC24700.3004899080403557RNA PCR Primer, Index 40 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCT22890.27847020222849156No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTA20260.24647471809302046No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT16120.19610920314212685No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGT10900.1326048582040436No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA8300.10097434156821669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTG453.8380676E-1045.07
TCGCGCG207.032113E-445.01
CGTTTTT287650.044.6167221
CGATGAA3950.042.7215219
TACGGCT15450.042.5242737
CCGATGA4050.042.22222518
TGATACC16450.042.127664
GATACCT16450.042.127665
CGACCTG1550.042.09677512
ACGGCTG15800.041.7246868
ATACGGT603.6379788E-1241.2499966
GCGACCT1650.040.9090911
CGGCTGT16300.040.8588949
ACGGTGA501.0804797E-940.58
CGCTTTT6250.040.321
ACCCGAA451.9272193E-840.039
ATACCTG17450.039.8424076
GAATGAT57650.039.614051
ATGATAC54200.039.6033173
CGCGGGT800.039.3754