##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933308.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	821991
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.813182869398812	31.0	28.0	33.0	25.0	34.0
2	29.401964255083083	31.0	30.0	33.0	16.0	34.0
3	29.30858853685746	31.0	28.0	34.0	16.0	34.0
4	33.589544167758525	35.0	32.0	37.0	28.0	37.0
5	34.48623403419259	35.0	35.0	37.0	32.0	37.0
6	34.145572153466404	35.0	35.0	37.0	29.0	37.0
7	33.99656565582835	35.0	33.0	37.0	30.0	37.0
8	34.041945714734105	35.0	35.0	37.0	29.0	37.0
9	35.192266095370876	37.0	34.0	39.0	30.0	39.0
10	35.08900462413822	37.0	34.0	39.0	28.0	39.0
11	35.46134203415853	37.0	34.0	39.0	30.0	39.0
12	35.579301963160184	37.0	34.0	39.0	30.0	39.0
13	35.82717937301017	37.0	35.0	39.0	30.0	39.0
14	36.59698463851794	38.0	35.0	41.0	30.0	41.0
15	36.980544799152305	39.0	36.0	41.0	31.0	41.0
16	36.89654144631754	39.0	35.0	41.0	31.0	41.0
17	36.14420717501773	38.0	34.0	40.0	29.0	41.0
18	35.855870684715526	38.0	34.0	39.0	29.0	40.0
19	35.076416895075496	37.0	34.0	38.0	27.0	40.0
20	34.28006146052694	35.0	33.0	38.0	27.0	40.0
21	34.432602060119876	35.0	33.0	38.0	29.0	40.0
22	34.81714519988662	35.0	33.0	39.0	30.0	40.0
23	35.18199590993089	35.0	34.0	39.0	30.0	40.0
24	34.53449855290386	35.0	33.0	38.0	27.0	40.0
25	33.47288838928893	35.0	33.0	38.0	24.0	40.0
26	33.479348314032634	35.0	32.0	38.0	24.0	40.0
27	34.33895018315285	35.0	33.0	38.0	27.0	40.0
28	34.089206572821354	35.0	33.0	39.0	25.0	40.0
29	33.97531359832407	35.0	33.0	39.0	24.0	40.0
30	33.058479959026315	35.0	33.0	38.0	21.0	40.0
31	32.91271193966844	35.0	33.0	38.0	21.0	40.0
32	32.039976106794356	35.0	31.0	38.0	15.0	40.0
33	30.942257275322966	35.0	27.0	39.0	10.0	40.0
34	30.004838252486948	35.0	22.0	39.0	7.0	40.0
35	29.32818850814668	35.0	19.0	39.0	7.0	41.0
36	28.640487547917193	35.0	17.0	39.0	7.0	41.0
37	28.400627257476057	35.0	15.0	39.0	7.0	40.0
38	28.197110430649484	35.0	15.0	39.0	7.0	40.0
39	27.78368011328591	34.0	15.0	39.0	7.0	40.0
40	27.63369915242381	34.0	15.0	39.0	7.0	40.0
41	27.362575745963156	34.0	13.0	39.0	7.0	40.0
42	27.377929928673186	34.0	12.0	39.0	7.0	40.0
43	27.271218298010563	34.0	12.0	39.0	7.0	40.0
44	27.223341861407242	34.0	10.0	39.0	7.0	40.0
45	27.33881149550299	34.0	10.0	39.0	7.0	40.0
46	27.05628528779512	34.0	10.0	39.0	7.0	40.0
47	26.88443425779601	33.0	10.0	39.0	7.0	40.0
48	26.77638197985136	33.0	10.0	38.0	7.0	40.0
49	26.896456287234287	34.0	10.0	39.0	7.0	40.0
50	26.860957115102234	34.0	10.0	39.0	7.0	40.0
51	25.895177684427203	32.0	10.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	15.0
10	35.0
11	34.0
12	53.0
13	92.0
14	138.0
15	201.0
16	479.0
17	988.0
18	1967.0
19	3431.0
20	5848.0
21	8773.0
22	13175.0
23	19843.0
24	29544.0
25	43873.0
26	55031.0
27	52937.0
28	43972.0
29	36367.0
30	34102.0
31	35085.0
32	38679.0
33	44635.0
34	52037.0
35	63368.0
36	73184.0
37	83230.0
38	69787.0
39	11080.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.15242137687639	2.2204622678350496	30.965424195642044	45.66169215964651
2	45.858774609454365	11.799277607662372	29.833295011745868	12.508652771137395
3	10.153882463433298	12.015946646617785	64.76579427268668	13.064376617262234
4	8.89766432965811	2.360488131865191	74.3065313367178	14.4353162017589
5	16.124872413444916	2.8808101305245435	66.57055855842704	14.423758897603504
6	11.711563751914559	10.227727554194633	68.62106762726113	9.439641066629683
7	47.865122610831506	0.7861399942335136	49.358447963542176	1.9902894313928012
8	47.97984406155298	6.913092722426402	42.92711234064607	2.179950875374548
9	45.37032643909726	2.203187139518559	42.09498644145739	10.331499979926788
10	24.576181491038223	16.151150073419295	48.77170187994759	10.500966555594891
11	17.59325832034657	15.604915382285208	54.70984475499123	12.091981542376985
12	13.129462488032107	14.690671795676593	57.49174869311221	14.688117023179087
13	13.502945896001295	14.809772856393804	61.42330025511228	10.26398099249262
14	13.034327626458197	18.781592499187948	55.49014526923044	12.693934605123413
15	11.035400630907152	15.653577715571096	61.19337073033646	12.11765092318529
16	12.155364231481853	17.042035740050682	54.637337878395265	16.165262150072202
17	12.783473298369447	16.94458941764569	57.675205689600006	12.596731594384853
18	12.983232176508016	16.02462800687599	56.84770271207349	14.144437104542506
19	12.914861598241343	17.651896431956068	54.4822266910465	14.951015278756094
20	14.233002551122823	17.319532695613454	58.09309347669257	10.354371276571154
21	14.421569092605639	19.707393389951957	55.34354999020671	10.5274875272357
22	13.302335427030224	14.48434350254443	56.93638981448702	15.276931255938326
23	12.750504567568258	19.3696767969479	56.42008245832375	11.45973617716009
24	13.822535769856362	16.655413502094305	55.02006712968877	14.501983598360566
25	11.778231148516225	22.60706017462478	53.35423380547962	12.26047487137937
26	13.081895057245152	16.417454692326313	55.89319104467081	14.607459205757728
27	14.510499506685596	18.082923049035816	53.570051253602536	13.836526190676054
28	11.819959099308873	18.02270341159453	56.373974897535376	13.783362591561222
29	16.841546926912827	16.33120070657708	53.32771283383882	13.499539532671282
30	12.860968064127224	21.833937354545245	53.61287410689412	11.692220474433418
31	15.08860802612194	19.647538720010317	50.86977837956863	14.39407487429911
32	17.672456267769356	20.623218502392362	49.18168203788119	12.522643191957089
33	13.849786676496459	22.148904306738153	46.0284844967889	17.972824519976495
34	18.46480070949682	23.605611253651198	44.137466225299306	13.792121811552683
35	16.84398004357712	24.916696168206222	41.60228031693778	16.637043471278883
36	17.731824314378137	24.844675914943107	39.13582995434257	18.287669816336187
37	18.120028078166307	25.835197708977347	40.50275489634315	15.542019316513198
38	17.935476179179577	27.833029802029465	37.978639668804156	16.2528543499868
39	20.727599207290588	25.304534964494746	37.67656823493201	16.291297593282653
40	20.447790790896736	25.946269484702388	38.638865875660436	14.967073848740437
41	18.060173408224664	27.68959757466931	36.679233714237746	17.57099530286828
42	19.565177720923952	25.058911837234227	39.373423796610915	16.00248664523091
43	19.921629312243077	23.493201263760795	37.01792355390752	19.567245870088602
44	19.508729414312324	25.461835956841377	35.929955437468294	19.09947919137801
45	17.68681165608869	24.524112794422322	36.88860340320028	20.900472146288706
46	21.299503279232983	24.751974170033492	35.675451434383106	18.273071116350422
47	16.759794206992535	24.35184813459028	41.11882003574248	17.7695376226747
48	18.687187572613325	25.829723196482686	35.314376921401816	20.168712309502173
49	17.844964239267828	23.858290419238166	39.26746156588089	19.029283775613116
50	18.25701254636608	23.25487748649316	37.90564616887533	20.582463798265433
51	18.006158218277328	23.325681181424127	36.22472752134755	22.443433078950985
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37.0
1	1117.5
2	2198.0
3	30925.5
4	59653.0
5	45442.5
6	31232.0
7	29987.5
8	28743.0
9	28381.0
10	28019.0
11	27384.5
12	26750.0
13	25969.0
14	25188.0
15	24118.0
16	23048.0
17	21941.5
18	20835.0
19	19289.5
20	17744.0
21	16600.5
22	15457.0
23	14640.5
24	13824.0
25	13581.0
26	13486.5
27	13635.0
28	13852.5
29	14070.0
30	14959.5
31	15849.0
32	16912.0
33	17975.0
34	19482.5
35	20990.0
36	22017.0
37	23044.0
38	24788.5
39	26533.0
40	27629.0
41	28725.0
42	29500.0
43	30275.0
44	32330.0
45	34385.0
46	63774.5
47	93164.0
48	68682.0
49	44200.0
50	40520.0
51	36840.0
52	33084.0
53	29328.0
54	26908.5
55	24489.0
56	22101.5
57	19714.0
58	17676.0
59	15638.0
60	12997.0
61	10356.0
62	8526.5
63	6697.0
64	5401.0
65	4105.0
66	3213.0
67	2321.0
68	1860.0
69	1399.0
70	1069.5
71	740.0
72	621.0
73	502.0
74	436.0
75	359.5
76	349.0
77	248.0
78	147.0
79	99.5
80	52.0
81	34.5
82	17.0
83	12.0
84	7.0
85	6.0
86	5.0
87	3.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	821991.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.4913515030671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.90754989133751	47.66467093060957
2	7.773669859371476	8.938375727078474
3	3.653462403224725	6.3012747368109885
4	2.11178884498262	4.856383791486076
5	1.2572783718672322	3.6141316407111477
6	0.7277543555876134	2.510374887898534
7	0.45306930756749564	1.8233296771629877
8	0.29614059843371665	1.362041859110516
9	0.18145437984287252	0.9388851779985875
>10	0.5673035534100113	5.286689909951906
>50	0.032927747318689415	1.3607995182465094
>100	0.03292715533558461	3.4839018400095276
>500	0.002124332600222979	0.8033760881613345
>1k	0.0016994660801783832	2.2718762529609524
>5k	0.0	0.0
>10k+	8.497330400891916E-4	8.783887961802904
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29723	3.6159763306410895	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC	15862	1.929704826451871	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG	14411	1.753182212457314	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC	11926	1.4508674669187376	No Hit
GAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT	3658	0.44501703789944175	RNA PCR Primer, Index 40 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTC	2749	0.3344318855072622	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAGAAGCT	2708	0.32944399634545873	No Hit
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	2470	0.3004899080403557	RNA PCR Primer, Index 40 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCT	2289	0.27847020222849156	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTA	2026	0.24647471809302046	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT	1612	0.19610920314212685	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGT	1090	0.1326048582040436	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	830	0.10097434156821669	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.216558332147189E-4	0.0	0.0	0.04172795079264858	0.0
2	1.216558332147189E-4	0.0	0.0	0.4132648654304001	0.0
3	1.216558332147189E-4	0.0	0.0	0.4895430728560288	0.0
4	1.216558332147189E-4	0.0	0.0	1.057675813968766	0.0
5	1.216558332147189E-4	0.0	0.0	3.4486995599708514	0.0
6	1.216558332147189E-4	0.0	0.0	3.7931072238017203	0.0
7	1.216558332147189E-4	0.0	0.0	4.225715366713261	0.0
8	1.216558332147189E-4	0.0	0.0	4.7130686345714246	0.0
9	1.216558332147189E-4	0.0	0.0	4.821099014466095	0.0
10	1.216558332147189E-4	0.0	0.0	6.969176061538387	0.0
11	1.216558332147189E-4	0.0	0.0	7.133046468878613	0.0
12	1.216558332147189E-4	0.0	0.0	8.97345591375088	0.0
13	1.216558332147189E-4	0.0	0.0	9.1471804435815	0.0
14	1.216558332147189E-4	0.0	0.0	9.25654903764153	0.0
15	1.216558332147189E-4	0.0	0.0	9.595603844810952	0.0
16	1.216558332147189E-4	0.0	0.0	9.807771617937423	0.0
17	1.216558332147189E-4	0.0	0.0	9.923466315324621	0.0
18	1.216558332147189E-4	0.0	0.0	10.002907574413832	0.0
19	1.216558332147189E-4	0.0	0.0	10.498411783097382	0.0
20	1.216558332147189E-4	0.0	0.0	10.60972687048885	0.0
21	1.216558332147189E-4	0.0	0.0	10.686735012913767	0.0
22	1.216558332147189E-4	0.0	0.0	10.842697791095036	0.0
23	1.216558332147189E-4	0.0	0.0	10.93199317267464	0.0
24	1.216558332147189E-4	0.0	0.0	11.014719139260649	0.0
25	1.216558332147189E-4	0.0	0.0	11.085279522525186	0.0
26	1.216558332147189E-4	0.0	0.0	11.167275554111907	0.0
27	1.216558332147189E-4	0.0	0.0	11.28284859566589	0.0
28	1.216558332147189E-4	0.0	0.0	11.359005147258303	0.0
29	1.216558332147189E-4	0.0	0.0	11.442826016343245	0.0
30	1.216558332147189E-4	0.0	0.0	11.549274870406125	0.0
31	1.216558332147189E-4	0.0	0.0	11.640881712816807	0.0
32	1.216558332147189E-4	0.0	0.0	11.748547125211834	0.0
33	1.216558332147189E-4	0.0	0.0	11.851711271777916	0.0
34	1.216558332147189E-4	0.0	0.0	11.990642233309124	0.0
35	1.216558332147189E-4	0.0	0.0	12.115461118187426	0.0
36	1.216558332147189E-4	0.0	0.0	12.229330978076403	0.0
37	1.216558332147189E-4	0.0	0.0	12.356582979618999	0.0
38	1.216558332147189E-4	0.0	0.0	12.501353421144513	0.0
39	1.216558332147189E-4	0.0	0.0	12.674712983475489	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTG	45	3.8380676E-10	45.0	7
TCGCGCG	20	7.032113E-4	45.0	1
CGTTTTT	28765	0.0	44.616722	1
CGATGAA	395	0.0	42.72152	19
TACGGCT	1545	0.0	42.524273	7
CCGATGA	405	0.0	42.222225	18
TGATACC	1645	0.0	42.12766	4
GATACCT	1645	0.0	42.12766	5
CGACCTG	155	0.0	42.096775	12
ACGGCTG	1580	0.0	41.724686	8
ATACGGT	60	3.6379788E-12	41.249996	6
GCGACCT	165	0.0	40.90909	11
CGGCTGT	1630	0.0	40.858894	9
ACGGTGA	50	1.0804797E-9	40.5	8
CGCTTTT	625	0.0	40.32	1
ACCCGAA	45	1.9272193E-8	40.0	39
ATACCTG	1745	0.0	39.842407	6
GAATGAT	5765	0.0	39.61405	1
ATGATAC	5420	0.0	39.603317	3
CGCGGGT	80	0.0	39.375	4
>>END_MODULE