##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933306.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	801893
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.5140249384893	31.0	28.0	33.0	25.0	34.0
2	28.783285301156138	31.0	28.0	33.0	16.0	34.0
3	28.778218540378827	31.0	28.0	34.0	16.0	34.0
4	33.23415094033743	35.0	32.0	37.0	28.0	37.0
5	34.30102145797507	35.0	33.0	37.0	30.0	37.0
6	33.83015065601022	35.0	33.0	37.0	28.0	37.0
7	33.72723044096906	35.0	33.0	37.0	29.0	37.0
8	33.707633063264055	35.0	33.0	37.0	28.0	37.0
9	34.8010781987123	37.0	34.0	39.0	28.0	39.0
10	34.63761249942324	37.0	33.0	39.0	27.0	39.0
11	35.101972457672034	37.0	34.0	39.0	28.0	39.0
12	35.24170805830703	37.0	34.0	39.0	30.0	39.0
13	35.539419847785176	37.0	34.0	39.0	30.0	39.0
14	36.229705210046724	38.0	34.0	40.0	30.0	41.0
15	36.62973987801365	38.0	35.0	40.0	30.0	41.0
16	36.527483093130876	38.0	34.0	40.0	30.0	41.0
17	35.79755777890566	38.0	34.0	40.0	27.0	41.0
18	35.56548691658364	38.0	34.0	39.0	28.0	40.0
19	34.901831042296166	37.0	34.0	38.0	27.0	40.0
20	34.1582143752346	35.0	33.0	38.0	27.0	40.0
21	34.42807332150299	35.0	33.0	38.0	28.0	40.0
22	34.91399475989939	35.0	33.0	39.0	30.0	40.0
23	35.23555387065357	35.0	34.0	39.0	30.0	40.0
24	34.627597447539756	35.0	33.0	39.0	27.0	40.0
25	33.5154503156905	35.0	33.0	38.0	24.0	40.0
26	33.58962604736542	35.0	32.0	38.0	24.0	40.0
27	34.417731542737	35.0	33.0	39.0	27.0	40.0
28	34.094468962816734	35.0	33.0	39.0	25.0	40.0
29	34.15123214693232	35.0	33.0	39.0	24.0	40.0
30	33.22337020026362	35.0	33.0	39.0	21.0	40.0
31	33.23478319426657	35.0	33.0	39.0	21.0	40.0
32	32.47261043555687	35.0	31.0	39.0	16.0	40.0
33	31.644715691495	35.0	30.0	39.0	11.0	40.0
34	30.9468545055263	36.0	25.0	39.0	8.0	40.0
35	30.33776576176622	36.0	23.0	40.0	7.0	41.0
36	29.68644070967074	35.0	21.0	40.0	7.0	41.0
37	29.576094316822818	35.0	19.0	40.0	7.0	41.0
38	29.41185295295008	35.0	18.0	39.0	7.0	40.0
39	29.004994431925457	35.0	18.0	39.0	7.0	40.0
40	28.883881016544603	35.0	18.0	39.0	7.0	40.0
41	28.50757520018257	35.0	18.0	39.0	7.0	40.0
42	28.728895251610876	35.0	18.0	39.0	7.0	40.0
43	28.628491581794577	35.0	17.0	39.0	7.0	40.0
44	28.687767569987518	35.0	17.0	39.0	7.0	40.0
45	28.85455790236353	35.0	18.0	39.0	7.0	40.0
46	28.547420915259266	35.0	17.0	39.0	7.0	40.0
47	28.477421551254345	35.0	15.0	39.0	7.0	40.0
48	28.279972515036295	35.0	15.0	39.0	7.0	40.0
49	28.47646132339352	35.0	15.0	39.0	7.0	40.0
50	28.430830547217646	35.0	15.0	39.0	7.0	40.0
51	27.259086935538782	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	19.0
10	26.0
11	24.0
12	53.0
13	80.0
14	144.0
15	219.0
16	429.0
17	915.0
18	1870.0
19	3355.0
20	5677.0
21	8444.0
22	12458.0
23	18369.0
24	26978.0
25	38685.0
26	47306.0
27	44263.0
28	35740.0
29	30967.0
30	29939.0
31	33523.0
32	39642.0
33	47717.0
34	56799.0
35	69049.0
36	80494.0
37	87609.0
38	70444.0
39	10649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.673491351090483	2.5221569461262288	34.050677584166465	38.75367411861682
2	39.84733624062063	14.760572794624718	32.303062877466196	13.089028087288455
3	10.508883354761794	14.868567252738208	60.929575392228145	13.692974000271857
4	9.188133578918883	2.49397363488645	73.02857114353162	15.289321642663047
5	18.126982028774414	2.9380478442884526	63.09357981675859	15.841390310178541
6	12.154364734447116	12.109845079081623	66.02539241519753	9.710397771273724
7	52.18426897354136	0.7937467966424447	44.88741016569542	2.134574064120774
8	51.88036309083631	8.327544946769706	37.08399998503541	2.7080919773585754
9	48.93969644329106	2.274243571149767	35.973876813988895	12.812183171570272
10	27.11633597001096	15.216868085891758	44.69997867545919	12.966817268638083
11	19.94530442340811	17.213393806904413	48.88183336180762	13.959468407879855
12	14.138170553926772	15.257521888830555	53.38480320940574	17.21950434783693
13	14.56628253395403	16.170985405783565	58.15688626786866	11.105845792393747
14	14.135551750669977	21.33040193641795	50.50698784002354	14.027058472888527
15	11.826390802762962	17.27038395396892	57.826168828010715	13.077056415257395
16	12.675007762881082	18.774325252870394	49.52181899580118	19.028847988447335
17	13.47636155945993	18.74040551544907	53.03550473691627	14.74772818817473
18	13.771163983224694	18.122991471430726	51.89807118904891	16.207773356295665
19	13.59433241093263	19.979099455912447	49.1802522281651	17.246315904989817
20	15.375492740303256	18.911126546808614	53.858183074300435	11.855197638587692
21	15.400683133535273	21.60462804887934	50.627328084919064	12.367360732666329
22	14.067338161076353	15.527133919363306	52.44951633197945	17.956011587580885
23	14.250030864466956	21.3145644119602	51.60027584727638	12.835128876296464
24	15.82019047429021	18.351326174439734	49.31318766967663	16.51529568159343
25	12.36810896216827	25.405509213822793	48.1233780566734	14.103003767335542
26	13.669404770960714	17.645371639358366	51.67123294504379	17.01399064463713
27	16.41565645291828	19.445611821028493	48.006155434702634	16.132576291350592
28	12.494435043079314	18.989316529761453	52.383672135808645	16.132576291350592
29	15.300794495026143	16.87057999009843	51.56261496234536	16.266010552530076
30	13.031663825472975	20.3197932891296	49.419062144201284	17.229480741196145
31	14.86308023639064	18.140325454892363	46.21950808898444	20.777086219732556
32	15.363895183023171	23.369826148875227	45.94528197652305	15.320996691578554
33	14.081429816696241	18.9734790053037	45.24768267088003	21.697408507120027
34	16.220617962745653	20.954291906775595	42.6443428237932	20.180747306685557
35	14.477742042891009	21.806026489818468	39.31372390081969	24.402507566470838
36	15.735889950404855	21.305835067770886	41.99475491119139	20.963520070632864
37	15.52239513251768	25.271950247726316	39.27818299947749	19.927471620278517
38	17.35817621553	23.334285247533025	38.1906314184062	21.116907118530776
39	18.99679882478086	24.811040874530644	36.53667010436554	19.65549019632295
40	17.274000396561636	23.305852526459265	39.59605583288543	19.824091244093665
41	17.260906380277667	25.219948297341414	37.44414778530303	20.07499753707789
42	17.138196741959337	24.261341600437962	38.66887477506351	19.931586882539193
43	19.39323575589262	22.704151301981685	38.00856223960054	19.894050702525153
44	18.888305546999412	23.750051440778257	35.068893231391215	22.29274978083111
45	17.17485998755445	23.363590903025717	35.19659106638915	24.264958043030678
46	21.588541114587606	25.5944371630629	33.77832204545993	19.038699676889557
47	16.438477452727483	23.069661413679878	40.98277450981615	19.50908662377649
48	18.768962941439817	24.659898515138554	33.30606452481815	23.265074018603478
49	16.999649579183256	23.165684199762314	38.32967740085024	21.50498882020419
50	18.68590946672436	22.246359551710764	37.11417857494703	21.953552406617842
51	18.281740830759215	22.594785089781304	34.36181635205695	24.761657727402532
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	840.5
2	1649.0
3	21380.0
4	41111.0
5	32306.0
6	23501.0
7	23179.0
8	22857.0
9	22779.0
10	22701.0
11	22246.0
12	21791.0
13	21268.0
14	20745.0
15	19881.5
16	19018.0
17	18401.5
18	17785.0
19	16682.0
20	15579.0
21	14672.5
22	13766.0
23	13092.0
24	12418.0
25	12267.0
26	12191.0
27	12266.0
28	12883.5
29	13501.0
30	14285.5
31	15070.0
32	16087.5
33	17105.0
34	18825.0
35	20545.0
36	22567.5
37	24590.0
38	25722.5
39	26855.0
40	28219.5
41	29584.0
42	31602.0
43	33620.0
44	36268.0
45	38916.0
46	76855.0
47	114794.0
48	82613.5
49	50433.0
50	46017.0
51	41601.0
52	36677.5
53	31754.0
54	28806.0
55	25858.0
56	23058.5
57	20259.0
58	17630.5
59	15002.0
60	12601.0
61	10200.0
62	8315.5
63	6431.0
64	5048.5
65	3666.0
66	2916.5
67	2167.0
68	1638.5
69	1110.0
70	869.0
71	628.0
72	488.5
73	349.0
74	304.5
75	190.5
76	121.0
77	97.5
78	74.0
79	52.0
80	30.0
81	24.5
82	19.0
83	13.0
84	7.0
85	6.0
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	801893.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.60325527896874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.03995673092825	42.8513208554061
2	6.406944369150091	6.612383716788071
3	3.099244540943216	4.797933216547292
4	1.9317177641845737	3.987316996485412
5	1.331102918482718	3.4344621852522
6	0.9594362032321729	2.9706018791564612
7	0.6980171240221158	2.521396908800338
8	0.5234501768400454	2.1609386481038553
9	0.42151043365682894	1.9576179459667948
>10	1.5111922793634844	11.605674612582888
>50	0.04772268120264087	1.701485326400054
>100	0.025565587617909	2.644314736605004
>500	0.0012174089340974861	0.405580536417118
>1k	0.0017043725077364805	2.3558349001923675
>5k	2.434817868194972E-4	0.7449464065728292
>10k+	9.739271472779888E-4	9.248191128723168
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTC	21511	2.68252746937559	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20770	2.5901211258858727	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCG	16704	2.083070933403833	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGC	14621	1.8233105913133048	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCT	5929	0.7393754528347298	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTC	3744	0.466895209211204	No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	3219	0.40142512779136363	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCATCCTT	3047	0.3799758820690541	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCT	2768	0.3451832102287961	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCATCCTTCGTA	2661	0.3318397841108477	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTT	2004	0.2499086536483047	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCATCCTTCGT	1307	0.1629893265061548	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05312429463781328	0.0
2	0.0	0.0	0.0	0.5151560120864006	0.0
3	0.0	0.0	0.0	0.6020753392285504	0.0
4	0.0	0.0	0.0	1.4914708072024572	0.0
5	0.0	0.0	0.0	4.7258175342595585	0.0
6	0.0	0.0	0.0	5.107663990083465	0.0
7	0.0	0.0	0.0	5.612095379308711	0.0
8	0.0	0.0	0.0	6.247466931373637	0.0
9	0.0	0.0	0.0	6.359451946830812	0.0
10	0.0	0.0	0.0	8.969276449601132	0.0
11	0.0	0.0	0.0	9.141743349798539	0.0
12	0.0	0.0	0.0	11.432572674907998	0.0
13	0.0	0.0	0.0	11.641952230534498	0.0
14	0.0	0.0	0.0	11.78112291789553	0.0
15	0.0	0.0	0.0	12.22282773387472	0.0
16	0.0	0.0	0.0	12.477101059617679	0.0
17	0.0	0.0	0.0	12.606669468370468	0.0
18	0.0	0.0	0.0	12.70219343478494	0.0
19	0.0	0.0	0.0	13.278205446362545	0.0
20	0.0	0.0	0.0	13.414009100964842	0.0
21	0.0	0.0	0.0	13.520257690240468	0.0
22	0.0	0.0	0.0	13.758444081691698	0.0
23	0.0	0.0	0.0	13.873172605322655	0.0
24	0.0	0.0	0.0	13.988275243704583	0.0
25	0.0	0.0	0.0	14.081928636364204	0.0
26	0.0	0.0	0.0	14.201645356674769	0.0
27	0.0	0.0	0.0	14.352039486564916	0.0
28	0.0	0.0	0.0	14.453175174243945	0.0
29	0.0	0.0	0.0	14.564037845448208	0.0
30	0.0	0.0	0.0	14.689241582106341	0.0
31	0.0	0.0	0.0	14.817687646606219	0.0
32	0.0	0.0	0.0	14.957481858552201	0.0
33	0.0	0.0	0.0	15.090791414814694	0.0
34	0.0	0.0	0.0	15.256150134743663	0.0
35	0.0	0.0	0.0	15.411283051479437	0.0
36	0.0	0.0	0.0	15.559183083029781	0.0
37	0.0	0.0	0.0	15.721673589867974	0.0
38	0.0	0.0	0.0	15.886782899962963	0.0
39	0.0	0.0	0.0	16.072094406610358	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCTCG	30	2.1646774E-6	45.000004	1
CGGCTAT	30	2.1646774E-6	45.000004	31
AAGACGC	25	3.8897466E-5	45.0	39
CTGCGCG	20	7.03201E-4	45.0	1
ATCCGGC	20	7.03201E-4	45.0	6
TCGTAAC	25	3.8897466E-5	45.0	34
CGTTTTT	22705	0.0	44.64325	1
CGATGAA	540	0.0	44.166668	19
CGGCTGT	1890	0.0	42.61905	9
CCGATGA	555	0.0	42.567566	18
ACGGCTG	1880	0.0	42.486702	8
TACGGCT	1905	0.0	42.04724	7
ACGGTGA	70	0.0	41.785717	8
GATACCT	2135	0.0	41.100704	5
TGATACC	2155	0.0	40.61485	4
ATACCTG	2180	0.0	40.561928	6
CGCTTTT	555	0.0	40.54054	1
GCGATCG	50	1.0804797E-9	40.5	9
GAATGAT	6965	0.0	40.05743	1
TGATCCG	45	1.9270374E-8	40.0	4
>>END_MODULE