##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933304.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 565073 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.48561159354632 31.0 28.0 33.0 25.0 34.0 2 28.732326619746477 31.0 28.0 33.0 16.0 34.0 3 28.787938903469108 31.0 28.0 34.0 16.0 34.0 4 33.14458839831314 35.0 32.0 37.0 28.0 37.0 5 34.244216234008704 35.0 33.0 37.0 30.0 37.0 6 33.665648509130676 35.0 33.0 37.0 28.0 37.0 7 33.51975408487045 35.0 32.0 37.0 29.0 37.0 8 33.5250772908987 35.0 33.0 37.0 28.0 37.0 9 34.67646304105841 37.0 34.0 39.0 28.0 39.0 10 34.41855831016524 37.0 32.0 39.0 27.0 39.0 11 34.751943554195655 37.0 34.0 39.0 27.0 39.0 12 34.816807739884936 37.0 34.0 39.0 27.0 39.0 13 35.092526098397904 37.0 34.0 39.0 28.0 39.0 14 35.88668012805425 38.0 34.0 40.0 27.0 41.0 15 36.20885620088024 38.0 34.0 40.0 30.0 41.0 16 36.05142698377024 38.0 34.0 40.0 30.0 41.0 17 35.360070291803005 37.0 33.0 40.0 27.0 41.0 18 35.12205325683584 37.0 33.0 39.0 27.0 40.0 19 34.498824045742765 37.0 33.0 38.0 27.0 40.0 20 34.04518885170589 35.0 32.0 38.0 27.0 40.0 21 34.46470986934432 35.0 33.0 38.0 27.0 40.0 22 35.01646689896704 35.0 33.0 39.0 30.0 40.0 23 35.32780189462247 36.0 34.0 39.0 30.0 40.0 24 34.84510496873855 35.0 33.0 39.0 28.0 40.0 25 33.828034607917914 35.0 33.0 39.0 24.0 40.0 26 33.892822697244426 35.0 33.0 38.0 25.0 40.0 27 34.60010122585931 35.0 33.0 39.0 27.0 40.0 28 34.39728849192936 35.0 33.0 39.0 25.0 40.0 29 34.46456121598448 35.0 33.0 39.0 25.0 40.0 30 33.75006592068635 35.0 33.0 39.0 23.0 40.0 31 33.78182641888747 35.0 33.0 39.0 23.0 40.0 32 33.300492148802014 35.0 33.0 39.0 20.0 40.0 33 32.822182266715984 36.0 32.0 39.0 15.0 40.0 34 32.41159814749599 36.0 31.0 40.0 12.0 41.0 35 32.02190690406372 36.0 31.0 40.0 10.0 41.0 36 31.500052205644227 36.0 30.0 40.0 8.0 41.0 37 31.482691616835346 36.0 30.0 40.0 8.0 41.0 38 31.008582961847406 35.0 28.0 39.0 8.0 41.0 39 30.772893059834747 35.0 27.0 39.0 8.0 40.0 40 30.39025046321449 35.0 24.0 39.0 8.0 40.0 41 30.088045261408702 35.0 23.0 39.0 7.0 40.0 42 30.268669711700966 35.0 24.0 39.0 7.0 40.0 43 30.135724056891764 35.0 23.0 39.0 7.0 40.0 44 30.223151698984026 35.0 23.0 39.0 7.0 40.0 45 30.357196326846267 35.0 24.0 39.0 7.0 40.0 46 30.161848115199277 35.0 23.0 39.0 7.0 40.0 47 30.090195426077692 35.0 23.0 39.0 7.0 40.0 48 29.862801797289908 35.0 23.0 39.0 7.0 40.0 49 30.061807943398463 35.0 24.0 39.0 7.0 40.0 50 30.070413911122987 35.0 24.0 39.0 7.0 40.0 51 28.730737444542562 34.0 22.0 38.0 7.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 6.0 9 27.0 10 20.0 11 43.0 12 54.0 13 37.0 14 81.0 15 141.0 16 256.0 17 507.0 18 1080.0 19 1794.0 20 2862.0 21 4437.0 22 6283.0 23 8888.0 24 13058.0 25 19007.0 26 24665.0 27 27001.0 28 25911.0 29 24276.0 30 25281.0 31 27673.0 32 32586.0 33 39038.0 34 45009.0 35 52734.0 36 59832.0 37 64339.0 38 50637.0 39 7509.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.573203816144108 2.60338044818988 35.102544273040834 35.72087146262518 2 34.14850824583726 16.060579783497 35.898370652995276 13.89254131767046 3 12.790559803777565 16.1219877785702 56.93459075199133 14.152861665660895 4 11.489665936967436 2.730797613759638 69.09620526905374 16.683331180219195 5 21.868324977480786 3.471763825204885 57.419660822583985 17.240250374730344 6 13.890594666529813 14.546085196072012 60.050294386742955 11.513025750655224 7 55.904989266873486 1.2157721214781099 40.13410656676217 2.745132044886236 8 55.78429689615324 10.702334034717637 30.267947681096068 3.2454213880330505 9 52.82273263808393 2.7442472034586682 29.73261861741757 14.700401541039831 10 32.03869234594469 17.24396670872613 36.45741346693259 14.259927478396598 11 24.891651167194325 16.9707276758932 43.29281349489358 14.844807662018889 12 18.54432967067972 15.378367042842253 46.44355685017688 19.63374643630115 13 17.76885464355933 15.968556275029952 52.62488209487978 13.637706986530942 14 15.637094676263066 21.268579457875354 45.40740753849503 17.686918327366552 15 13.363937048841477 16.309220224643543 53.95338301423001 16.37345971228496 16 15.14069863539755 17.67293783281098 44.45177879672184 22.734584735069628 17 15.392878442254363 18.208974769631535 48.96411614074642 17.434030647367685 18 15.225466444158542 17.21812933904115 48.10351936829401 19.4528848485063 19 15.321383254906888 20.16111192713154 44.406120979059345 20.11138383890223 20 18.15747699854709 18.214637754767967 49.62279209942786 14.00509314725708 21 18.11872094401962 22.99171965392082 44.49354331210304 14.396016089956518 22 16.09650434545625 15.846625126311114 47.653135081662015 20.40373544657062 23 16.976567629315152 21.747632606760543 45.784703923209925 15.491095840714387 24 17.44765720535223 17.93095759308974 44.64520513278815 19.976180068769875 25 14.725354069297241 24.917311568593792 43.31617330858137 17.0411610535276 26 15.286874439231745 17.08699583947561 48.09520185887487 19.530927862417776 27 18.066338331507612 18.974008667906624 43.340240995411214 19.619412005174553 28 13.292264893208488 19.72028392791728 48.65760707023694 18.329844108637293 29 18.695283618222778 16.317714702348194 46.67396955791553 18.313032121513505 30 17.951839850780342 21.316006958392986 45.01312219837083 15.719030992455844 31 19.027983994988258 17.135308181420807 41.46030689840074 22.376400925190197 32 22.407193406869556 20.462665885646633 41.49800114321513 15.632139564268687 33 17.91060624025568 18.34081614233913 41.09504435710076 22.65353326030442 34 21.702505693954585 20.078113801225683 41.2835155811727 16.935864923647035 35 20.47204520477885 21.061880500395525 40.00580455976485 18.46026973506078 36 18.167564190821363 21.020115985014325 40.22471432894511 20.5876054952192 37 23.942570251985142 21.538102156712498 37.75087466575115 16.768452925551212 38 18.61812544573887 22.650524799450693 37.74025656862034 20.991093186190103 39 25.43759832800364 22.34277695094262 35.14537059813511 17.07425412291863 40 18.434609333661314 21.71365469594194 41.35324108566504 18.498494884731706 41 20.450278105660686 23.71233451253201 36.42025012697474 19.41713725483256 42 18.286132942115444 22.40542372401442 40.214096231814295 19.094347102055842 43 19.583487443215304 21.634726840602895 37.277484502002395 21.504301214179407 44 21.861777150916783 22.148465773448738 35.022908544559726 20.966848531074746 45 18.168272063963418 21.557037763262446 36.07976314564667 24.19492702712747 46 23.242306746208012 23.74277305764034 33.98392774030966 19.03099245584199 47 16.790220024669377 20.94561233681312 42.9079074739016 19.3562601646159 48 21.03586616242503 23.090467957237383 33.85084758960347 22.02281829073412 49 18.257287111576733 20.88385040516889 39.80494555570696 21.053916927547416 50 18.868535569740548 20.53132958042589 37.95898936951509 22.64114548031847 51 19.771250794145182 20.861021496337642 35.59203855077131 23.775689158745863 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 31.0 1 675.0 2 1319.0 3 16050.0 4 30781.0 5 23395.0 6 16009.0 7 15547.5 8 15086.0 9 14577.5 10 14069.0 11 13405.0 12 12741.0 13 11974.0 14 11207.0 15 10370.5 16 9534.0 17 8736.5 18 7939.0 19 7300.0 20 6661.0 21 6413.5 22 6166.0 23 5805.0 24 5444.0 25 5634.0 26 6008.5 27 6193.0 28 6645.5 29 7098.0 30 7923.0 31 8748.0 32 9328.5 33 9909.0 34 11379.0 35 12849.0 36 13543.5 37 14238.0 38 14905.5 39 15573.0 40 16476.0 41 17379.0 42 18720.5 43 20062.0 44 21980.0 45 23898.0 46 52002.0 47 80106.0 48 58507.0 49 36908.0 50 34625.5 51 32343.0 52 28860.5 53 25378.0 54 24429.0 55 23480.0 56 22324.5 57 21169.0 58 19604.0 59 18039.0 60 16323.5 61 14608.0 62 12750.0 63 10892.0 64 9396.5 65 7901.0 66 6567.5 67 5234.0 68 4505.5 69 3777.0 70 3107.0 71 2437.0 72 2061.5 73 1686.0 74 1358.0 75 860.5 76 691.0 77 511.0 78 331.0 79 250.5 80 170.0 81 119.5 82 69.0 83 50.5 84 32.0 85 26.5 86 21.0 87 14.0 88 7.0 89 6.0 90 5.0 91 3.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 565073.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.678125144314215 #Duplication Level Percentage of deduplicated Percentage of total 1 83.34339532708603 34.735964603943444 2 5.228023597543409 4.35788443511684 3 2.1277659937530697 2.660438920963696 4 1.282701174049203 2.138423202191258 5 1.0239101439987404 2.1337327559056143 6 0.8443322227862273 2.1114110426796824 7 0.7549024293507892 2.202404254556031 8 0.6551816872943439 2.1845395484253287 9 0.5713578021238811 2.143180978118949 >10 4.058446430855812 27.76227990903103 >50 0.07432457840058469 2.1089489909031514 >100 0.03007352883195342 2.5023401381707737 >500 8.592436809129549E-4 0.2669759675143201 >1k 0.0030073528831953424 3.2373745758946395 >5k 0.0 0.0 >10k+ 0.0017184873618259098 9.454100676585236 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG 15631 2.7661912708623486 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13690 2.4226958286805425 No Hit GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC 12037 2.130167252726639 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC 11441 2.0246941545605615 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCGTGTTT 3524 0.6236362381497612 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC 2971 0.5257727762607664 No Hit GAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT 2944 0.5209946325519004 No Hit GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC 2655 0.4698507980384835 TruSeq Adapter, Index 14 (95% over 21bp) GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTA 2598 0.45976360576421105 No Hit GAATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT 2336 0.4133979149596601 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGT 1052 0.18617063636025788 No Hit GAATGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTT 767 0.13573467498889524 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTAT 724 0.12812503871181244 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.08423690390445128 0.0 2 0.0 0.0 0.0 0.6712407069529069 0.0 3 0.0 0.0 0.0 0.7653878348461173 0.0 4 0.0 0.0 0.0 1.4095523941154506 0.0 5 0.0 0.0 0.0 4.030629670856686 0.0 6 0.0 0.0 0.0 4.261750251737386 0.0 7 0.0 0.0 0.0 4.816014921965834 0.0 8 0.0 0.0 0.0 5.519463856882208 0.0 9 0.0 0.0 0.0 5.629361162186124 0.0 10 0.0 0.0 0.0 9.092630509686359 0.0 11 0.0 0.0 0.0 9.322866249139492 0.0 12 0.0 0.0 0.0 11.891030008512175 0.0 13 0.0 0.0 0.0 12.086933900575678 0.0 14 0.0 0.0 0.0 12.278413585501342 0.0 15 0.0 0.0 0.0 12.893555345946453 0.0 16 0.0 0.0 0.0 13.201834099311062 0.0 17 0.0 0.0 0.0 13.373670304544722 0.0 18 0.0 0.0 0.0 13.512767376958376 0.0 19 0.0 0.0 0.0 14.401502106807438 0.0 20 0.0 0.0 0.0 14.60129930115224 0.0 21 0.0 0.0 0.0 14.732432800717783 0.0 22 0.0 0.0 0.0 15.036287346944555 0.0 23 0.0 0.0 0.0 15.18848007248621 0.0 24 0.0 0.0 0.0 15.32350687433305 0.0 25 0.0 0.0 0.0 15.43853625991686 0.0 26 0.0 0.0 0.0 15.573740030049215 0.0 27 0.0 0.0 0.0 15.742036869572605 0.0 28 0.0 0.0 0.0 15.86184439886528 0.0 29 0.0 0.0 0.0 16.003595995561636 0.0 30 0.0 0.0 0.0 16.156496594245347 0.0 31 0.0 0.0 0.0 16.28214407695997 0.0 32 0.0 0.0 0.0 16.424780515083892 0.0 33 0.0 0.0 0.0 16.567239984922303 0.0 34 0.0 0.0 0.0 16.77075351326289 0.0 35 0.0 0.0 0.0 16.9406430673559 0.0 36 0.0 0.0 0.0 17.104869636312475 0.0 37 0.0 0.0 0.0 17.274228285548947 0.0 38 0.0 0.0 0.0 17.45420503191623 0.0 39 0.0 0.0 0.0 17.65807249682784 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTA 45 3.8380676E-10 45.000004 6 TTGCGAT 20 7.03024E-4 45.0 17 ATACCGA 20 7.03024E-4 45.0 13 ACATACG 60 0.0 44.999996 17 CGTTTTT 12865 0.0 44.160515 1 GATACCT 1635 0.0 42.66055 5 TGATACC 1630 0.0 42.653378 4 TATGGGC 85 0.0 42.35294 4 TACGGCT 1515 0.0 42.17822 7 ACGGCTG 1525 0.0 41.90164 8 CGGCTGT 1560 0.0 41.25 9 ACCCGTC 100 0.0 40.5 17 CACCCGT 100 0.0 40.5 16 GGCGACT 445 0.0 40.44944 10 ATGATAC 5740 0.0 40.374565 3 ACACGCG 145 0.0 40.34483 36 CCGATGA 415 0.0 40.12048 18 ATACGGC 4070 0.0 40.024567 6 AATGATA 5690 0.0 39.8594 2 GAATGAT 5975 0.0 39.80335 1 >>END_MODULE