##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933303.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394374
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.6167571898756	31.0	28.0	31.0	16.0	33.0
2	28.910937333597044	31.0	30.0	33.0	16.0	34.0
3	29.11419109779042	31.0	30.0	33.0	16.0	34.0
4	32.075332045215966	35.0	30.0	37.0	19.0	37.0
5	33.67273451089575	35.0	33.0	37.0	28.0	37.0
6	32.78690532337325	35.0	33.0	37.0	17.0	37.0
7	32.048093941284165	35.0	31.0	35.0	25.0	37.0
8	32.93019570255645	35.0	32.0	35.0	27.0	37.0
9	32.879175604882676	35.0	31.0	37.0	25.0	39.0
10	31.21099514673888	35.0	27.0	39.0	15.0	39.0
11	32.13176325011284	35.0	27.0	39.0	15.0	39.0
12	32.94165944002393	35.0	31.0	39.0	22.0	39.0
13	33.23841074715879	35.0	31.0	39.0	24.0	39.0
14	33.445364045297104	36.0	31.0	39.0	19.0	41.0
15	34.04340803399818	36.0	32.0	40.0	25.0	41.0
16	34.146234285221645	36.0	32.0	40.0	25.0	41.0
17	32.05993042137666	35.0	27.0	39.0	18.0	41.0
18	32.84486046240371	36.0	30.0	39.0	21.0	39.0
19	32.665736077936174	36.0	30.0	37.0	19.0	39.0
20	32.42708190702227	34.0	30.0	37.0	24.0	39.0
21	32.81294659384239	35.0	31.0	37.0	24.0	39.0
22	33.11724403738583	35.0	31.0	38.0	25.0	39.0
23	34.128502386059935	35.0	33.0	38.0	27.0	40.0
24	32.41633576249956	35.0	31.0	37.0	22.0	39.0
25	31.63218417035606	35.0	30.0	37.0	18.0	39.0
26	30.93218619888735	33.0	26.0	37.0	20.0	39.0
27	32.592260138852964	35.0	31.0	38.0	22.0	39.0
28	32.620636755972754	35.0	31.0	38.0	22.0	39.0
29	32.87707353933069	35.0	31.0	38.0	22.0	40.0
30	31.802403302448944	35.0	30.0	38.0	18.0	40.0
31	32.13499115053224	35.0	30.0	38.0	21.0	40.0
32	32.06571427122478	35.0	30.0	38.0	19.0	40.0
33	32.15730753041529	35.0	31.0	38.0	18.0	40.0
34	31.38217783119577	35.0	30.0	38.0	14.0	40.0
35	30.939151668213423	35.0	29.0	38.0	10.0	40.0
36	30.303792846384397	35.0	27.0	38.0	9.0	40.0
37	30.424574135211753	35.0	27.0	38.0	8.0	40.0
38	30.10666017536653	35.0	25.0	38.0	8.0	40.0
39	29.757740114713446	35.0	25.0	38.0	8.0	40.0
40	29.338957436342152	34.0	23.0	38.0	8.0	40.0
41	28.98742817731392	34.0	23.0	38.0	8.0	40.0
42	29.4554331675009	34.0	23.0	38.0	8.0	40.0
43	29.34423161770299	34.0	23.0	38.0	7.0	40.0
44	29.353742893801314	34.0	23.0	38.0	7.0	40.0
45	29.620938500002534	35.0	23.0	38.0	7.0	40.0
46	29.337332075643932	34.0	23.0	38.0	7.0	40.0
47	29.376137879271962	35.0	23.0	38.0	7.0	40.0
48	29.105506448193847	34.0	23.0	38.0	7.0	40.0
49	28.94672569692728	34.0	23.0	37.0	7.0	40.0
50	29.14222032892635	34.0	23.0	38.0	7.0	40.0
51	27.455704483561288	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	13.0
10	14.0
11	20.0
12	33.0
13	46.0
14	82.0
15	212.0
16	329.0
17	653.0
18	1197.0
19	1958.0
20	2939.0
21	4390.0
22	5925.0
23	8306.0
24	11107.0
25	15266.0
26	19618.0
27	21803.0
28	21719.0
29	22496.0
30	24361.0
31	28027.0
32	32085.0
33	35936.0
34	40375.0
35	43498.0
36	32216.0
37	16350.0
38	3336.0
39	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.07244899511631	2.529324955499095	36.921298057174155	38.47692799221044
2	38.18228382195581	11.145258054537063	37.1294760810804	13.542982042426733
3	13.077687677179531	10.997175270175012	61.93689239148625	13.988244661159206
4	12.032486928651483	2.7453635381642805	68.57373964815125	16.64840988503299
5	18.604421183952287	3.3234949565640735	61.5550213756485	16.51706248383514
6	13.862982853839249	10.386080218269967	63.33784681545944	12.413090112431346
7	54.880139157246674	1.2039333221764112	40.91268694183693	3.003240578739978
8	56.68502487486498	7.555518365815191	32.188227418643216	3.571229340676617
9	52.78872339454427	2.593223691216967	32.737705832534594	11.88034708170417
10	29.95202523492928	17.019377545172855	38.750779716715606	14.277817503182257
11	22.67162642567715	16.9648607666834	46.23631375293503	14.127199054704418
12	18.357193932662906	15.402638104946067	48.67384766744258	17.56632029494845
13	16.77012176259084	16.076871193334245	53.73503324255656	13.417973801518357
14	14.839213538417848	18.21976093758716	48.92538554772881	18.015639976266186
15	13.02038166816271	16.103495666550028	54.78403748725829	16.092085178028977
16	14.604664607707404	15.764223807857517	47.634986079204005	21.996125505231078
17	14.981717861725164	16.539122761642503	50.887482440526	17.591676936106335
18	14.79230375227576	15.63642633642177	50.82256943916181	18.74870047214066
19	15.170117705528256	18.900079619853237	47.06598305162105	18.86381962299746
20	17.448411913564282	17.14261082120018	51.49097050008368	13.91800676515186
21	17.83231146069467	20.531272345540017	46.65621973050962	14.980196463255691
22	15.857789813730113	16.025143645372157	48.903578836333025	19.213487704564702
23	16.259439009671024	20.172983005979095	47.0989466851263	16.46863129922358
24	16.136966432878435	16.842134623479236	47.65831418906926	19.36258475457307
25	14.426407420367468	20.80816686698413	46.426234995207594	18.339190717440804
26	14.718515926506312	17.258490671291717	50.13819369431047	17.884799707891492
27	15.886696384650104	17.972787252709356	46.70008671971276	19.44042964292778
28	13.145136342659505	18.26033156343978	50.852490275728115	17.742041818172595
29	17.58685917428634	15.388184819486073	48.83587660444147	18.18907940178612
30	16.48790234650357	19.54413830526353	47.81349683295552	16.154462515277377
31	17.88124977812939	17.005431392536018	44.9606211362818	20.152697693052787
32	19.64429703783718	19.314407136373088	45.0278162353502	16.013479590439534
33	16.827174205196084	17.611962249032644	44.423060343734626	21.137803202036647
34	19.149335402435252	19.35599202787202	44.427624539143046	17.06704803054968
35	18.961442691455318	20.203664541780135	42.50914106913742	18.325751697627126
36	17.66115413287894	19.95719798972549	42.18736529284385	20.194282584551722
37	22.0306105372058	20.732604076333633	40.33354125779083	16.90324412866974
38	18.208857581889273	21.565569738370176	41.19363852586631	19.031934153874243
39	22.962720666169677	21.12588558069244	39.130368634849155	16.78102511828873
40	18.50908021319864	20.58832478814526	43.06191584637932	17.840679152276774
41	18.91681500301744	21.567598269662806	40.67915227677281	18.836434450546943
42	17.45322967538428	21.05285845415773	42.295384584176446	19.19852728628155
43	18.373422183003953	20.324108587280097	40.61753563875914	20.68493359095681
44	20.900211474387255	20.850258891306222	38.09733907407689	20.15219056022963
45	17.855893136971503	20.730068412217843	38.98000375278289	22.43403469802776
46	20.958785315461974	22.47232322617617	37.66044414692652	18.90844731143534
47	16.5834968836688	20.12277685648648	44.233646234285224	19.060080025559493
48	20.186929158615932	21.24303326284187	37.65993701410336	20.91010056443883
49	17.66368979699473	19.795929751961335	41.51363933727883	21.02674111376511
50	18.257542332912415	19.179509805413137	40.34266964860767	22.220278213066784
51	19.17621344206261	19.481507401603555	38.63921049562091	22.703068660712926
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53.0
1	362.5
2	672.0
3	18307.0
4	35942.0
5	23901.5
6	11861.0
7	11437.0
8	11013.0
9	10683.5
10	10354.0
11	9810.0
12	9266.0
13	8389.5
14	7513.0
15	6848.0
16	6183.0
17	5660.5
18	5138.0
19	4695.0
20	4252.0
21	3999.0
22	3746.0
23	3525.0
24	3304.0
25	3337.0
26	3496.0
27	3622.0
28	3870.0
29	4118.0
30	4720.0
31	5322.0
32	5860.0
33	6398.0
34	7341.0
35	8284.0
36	8653.0
37	9022.0
38	9592.0
39	10162.0
40	10828.0
41	11494.0
42	12844.5
43	14195.0
44	15492.5
45	16790.0
46	28197.0
47	39604.0
48	34559.0
49	29514.0
50	28006.5
51	26499.0
52	22968.0
53	19437.0
54	18012.0
55	16587.0
56	15450.5
57	14314.0
58	13234.5
59	12155.0
60	10908.0
61	9661.0
62	8501.5
63	7342.0
64	6469.0
65	5596.0
66	4680.5
67	3765.0
68	3284.0
69	2803.0
70	2334.0
71	1865.0
72	1567.0
73	1269.0
74	1046.0
75	675.0
76	527.0
77	413.0
78	299.0
79	222.5
80	146.0
81	94.5
82	43.0
83	35.0
84	27.0
85	22.5
86	18.0
87	9.5
88	1.0
89	3.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	394374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.79860584223858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.68680578382387	35.33969603194958
2	6.72446577120228	5.890444516250271
3	2.841734029618112	3.7339196601516007
4	1.809054797940578	3.169363121680398
5	1.3136286698994368	2.8767552167994785
6	1.0878088635252927	2.8586706987142683
7	0.8880002103922022	2.7225219841955095
8	0.715663444832834	2.507604890874556
9	0.6278804738624997	2.4750260451667447
>10	3.2154761883806677	21.693176382916292
>50	0.05180523324973889	1.5545207798615592
>100	0.03061218328393661	2.57582726668054
>500	0.0029434791619169817	0.9279682014798061
>1k	0.0029434791619169817	3.2758644067243505
>5k	5.886958323833963E-4	1.907246675839435
>10k+	5.886958323833963E-4	6.491394120715643
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25176	6.383787977909295	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	7397	1.8756307464488025	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	4528	1.1481487116290627	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	3477	0.8816504130596845	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCGTGTTT	1940	0.4919188384629818	No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	1592	0.40367772723353973	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	1168	0.29616556872410454	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC	967	0.24519871999675436	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTA	901	0.2284633368325498	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTGCGTGTTTCGTATGCCG	638	0.1617753705873105	No Hit
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	547	0.13870082713363457	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT	546	0.1384472607220557	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGT	414	0.10497649439364665	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	395	0.10015873257364835	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17749648810519963	0.0
2	0.0	0.0	0.0	0.9113176832144106	0.0
3	0.0	0.0	0.0	1.1179743086511789	0.0
4	0.0	0.0	0.0	1.7108125789225457	0.0
5	0.0	0.0	0.0	3.0630822518725878	0.0
6	0.0	0.0	0.0	3.3714190083524778	0.0
7	0.0	0.0	0.0	3.726158418151298	0.0
8	0.0	0.0	0.0	4.265494175579526	0.0
9	0.0	0.0	0.0	4.4343694056910445	0.0
10	0.0	0.0	0.0	7.336944119034216	0.0
11	0.0	0.0	0.0	8.042112309635016	0.0
12	0.0	0.0	0.0	9.937774802598549	0.0
13	0.0	0.0	0.0	10.173084432543728	0.0
14	0.0	0.0	0.0	10.314828056616308	0.0
15	0.0	0.0	0.0	10.777586757747722	0.0
16	0.0	0.0	0.0	11.106462393565499	0.0
17	0.0	0.0	0.0	11.44548068584643	0.0
18	0.0	0.0	0.0	11.76928499343263	0.0
19	0.0	0.0	0.0	12.613407577578643	0.0
20	0.0	0.0	0.0	12.933661955402739	0.0
21	0.0	0.0	0.0	13.2795265407963	0.0
22	0.0	0.0	0.0	13.774741742609807	0.0
23	0.0	0.0	0.0	14.128466886762311	0.0
24	0.0	0.0	0.0	14.40181147844432	0.0
25	0.0	0.0	0.0	14.64574236638318	0.0
26	0.0	0.0	0.0	14.880291297093622	0.0
27	0.0	0.0	0.0	15.108501067514593	0.0
28	0.0	0.0	0.0	15.3374715371703	0.0
29	0.0	0.0	0.0	15.566949139649166	0.0
30	0.0	0.0	0.0	15.829390375633283	0.0
31	0.0	0.0	0.0	16.062925040697408	0.0
32	0.0	0.0	0.0	16.29620613934996	0.0
33	0.0	0.0	0.0	16.51554108536567	0.0
34	0.0	0.0	0.0	16.76327546947821	0.0
35	0.0	0.0	0.0	17.02343460775812	0.0
36	0.0	0.0	0.0	17.258490671291717	0.0
37	0.0	0.0	0.0	17.472247156252696	0.0
38	0.0	0.0	0.0	17.72124937242313	0.0
39	0.0	0.0	0.0	17.972787252709356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	20	7.027643E-4	45.000004	17
ATAAGCG	20	7.027643E-4	45.000004	33
TGCTACG	55	1.8189894E-12	45.000004	4
ATACAGC	20	7.027643E-4	45.000004	28
GTGCACT	25	3.8861293E-5	45.0	39
CGGGATC	85	0.0	45.0	6
CACCGGT	140	0.0	45.0	16
TATACGG	25	3.8861293E-5	45.0	27
CGTTTTT	10525	0.0	44.18765	1
GATACCT	1005	0.0	42.985073	5
TGATACC	1035	0.0	41.95652	4
TACGGCT	620	0.0	41.370968	7
TAGGGAT	60	3.6379788E-12	41.249996	5
GAATGAT	2925	0.0	41.23077	1
ATGATAC	2810	0.0	40.99644	3
ACATACG	55	6.002665E-11	40.909092	17
AATGATA	2790	0.0	40.887096	2
TGATACG	1785	0.0	40.714287	4
CGGGCTA	50	1.0768417E-9	40.5	6
CGGCTGT	640	0.0	40.42969	9
>>END_MODULE