##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933302.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	608412
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.521621861501746	31.0	28.0	33.0	25.0	34.0
2	28.87945339671144	31.0	28.0	33.0	16.0	34.0
3	28.86722648468472	31.0	28.0	34.0	16.0	34.0
4	33.23218476953117	35.0	32.0	37.0	28.0	37.0
5	34.271487084409905	35.0	33.0	37.0	30.0	37.0
6	33.84134928305161	35.0	33.0	37.0	28.0	37.0
7	33.690472903230045	35.0	33.0	37.0	29.0	37.0
8	33.66959231573342	35.0	33.0	37.0	28.0	37.0
9	34.84126052740577	37.0	34.0	39.0	28.0	39.0
10	34.61495664122338	37.0	33.0	39.0	27.0	39.0
11	34.75806032754121	37.0	34.0	39.0	27.0	39.0
12	34.8347682162745	37.0	34.0	39.0	27.0	39.0
13	34.905100491114574	37.0	34.0	39.0	27.0	39.0
14	35.80213408019566	38.0	34.0	40.0	27.0	41.0
15	36.12381576957719	38.0	34.0	40.0	27.0	41.0
16	36.02958357165868	38.0	34.0	40.0	27.0	41.0
17	35.36373214203533	37.0	33.0	40.0	27.0	41.0
18	35.08358316404016	37.0	33.0	39.0	27.0	40.0
19	34.337427598403714	37.0	33.0	37.0	27.0	40.0
20	33.641504441069536	35.0	32.0	37.0	27.0	39.0
21	33.93653478235143	35.0	33.0	38.0	27.0	40.0
22	34.43292538608706	35.0	33.0	38.0	28.0	40.0
23	34.83527116493429	35.0	33.0	39.0	30.0	40.0
24	34.27225465638416	35.0	33.0	38.0	27.0	40.0
25	33.404119248141065	35.0	33.0	38.0	24.0	40.0
26	33.27358763469491	35.0	32.0	38.0	24.0	40.0
27	34.03536748124626	35.0	33.0	38.0	25.0	40.0
28	33.81610980717014	35.0	33.0	38.0	24.0	40.0
29	33.86702431904696	35.0	33.0	39.0	24.0	40.0
30	33.09232395153284	35.0	33.0	38.0	21.0	40.0
31	32.93722510404134	35.0	32.0	38.0	21.0	40.0
32	32.206570547589465	35.0	31.0	38.0	16.0	40.0
33	31.535865499036838	35.0	30.0	39.0	12.0	40.0
34	30.810087572237233	35.0	27.0	39.0	8.0	40.0
35	30.291716797170338	35.0	24.0	39.0	7.0	40.0
36	29.676876853185014	35.0	22.0	39.0	7.0	40.0
37	29.575455447953033	35.0	21.0	39.0	7.0	40.0
38	29.308144809767064	35.0	21.0	39.0	7.0	40.0
39	28.896178905084057	35.0	20.0	38.0	7.0	40.0
40	28.658507721741188	34.0	18.0	38.0	7.0	40.0
41	28.091295043490266	34.0	18.0	38.0	7.0	40.0
42	28.312321255991005	34.0	18.0	38.0	7.0	40.0
43	28.188799694943558	34.0	18.0	38.0	7.0	40.0
44	28.239230981637444	34.0	18.0	38.0	7.0	40.0
45	28.403159372267478	34.0	18.0	38.0	7.0	40.0
46	28.284580185795154	34.0	18.0	38.0	7.0	40.0
47	28.254160009993228	34.0	18.0	38.0	7.0	40.0
48	28.137776703944038	34.0	18.0	38.0	7.0	40.0
49	28.298105560048125	34.0	18.0	38.0	7.0	40.0
50	28.265558535992056	34.0	18.0	38.0	7.0	40.0
51	27.0278413312032	33.0	18.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	39.0
10	35.0
11	61.0
12	44.0
13	89.0
14	133.0
15	196.0
16	339.0
17	680.0
18	1274.0
19	2400.0
20	3926.0
21	5864.0
22	8488.0
23	12454.0
24	18131.0
25	26528.0
26	33039.0
27	34754.0
28	32183.0
29	30057.0
30	30302.0
31	33082.0
32	37395.0
33	42499.0
34	46587.0
35	52961.0
36	56114.0
37	54824.0
38	38672.0
39	5249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.001972347685452	2.334930934958548	29.979684818839864	42.68341189851614
2	41.7253440103088	14.175755902250447	30.201080846531625	13.89781924090912
3	11.786421043634904	14.425422246766995	59.610264097355085	14.17789261224302
4	10.499464178878785	2.709841357501167	70.56024536005206	16.230449103567977
5	18.95689105408835	3.2185426980401437	60.727434698855376	17.09713154901613
6	13.33537142594163	11.939771076178642	63.95435987455868	10.770497623321038
7	49.947404061721336	1.2126651019375028	46.36003234650204	2.479898489839122
8	49.136111713772905	8.604202415468464	39.192520857576774	3.067165013181857
9	46.21900948699237	2.2568588390761524	38.351643294346594	13.172488379584887
10	29.959139530449765	13.907187892415006	44.291039624465	11.84263295267023
11	25.096151949665686	13.654069939448926	48.00990118538096	13.23987692550443
12	19.39540968948673	12.80168701472029	51.853185012787385	15.949718283005595
13	18.46331104580449	12.681045081293597	57.23128406408815	11.624359808813765
14	15.05230008612585	17.4503461470188	52.58246056948253	14.914893197372834
15	12.966049321841123	12.936628468866493	59.53038401609436	14.56693819319803
16	16.553914123981777	15.067421418380967	49.90384805033432	18.474816407302946
17	16.331367560140166	15.64663418867478	53.08590231619363	14.93609593499142
18	17.16106848648613	14.431996739051828	52.17484204782286	16.232092726639184
19	16.088275707908455	17.142166821167233	48.994102680420504	17.775454790503805
20	17.483547333057203	18.234025627370926	52.72364779129932	11.558779248272552
21	17.258535334608784	19.93221698454337	51.0137867103213	11.795460970526552
22	15.149109485020018	13.06433798149938	53.85183066737671	17.93472186610389
23	15.694956706968306	18.09431766631822	53.200134119642605	13.010591507070867
24	18.12784757697087	15.493612880745284	49.86785270507485	16.510686837208997
25	16.253459826564892	21.386823402562737	47.230495125013974	15.129221645858399
26	14.721274399584491	13.700913196978362	52.060938968988125	19.516873434449025
27	17.463988218509826	14.736231369532488	50.02777722990343	17.772003182054267
28	12.362017843172062	16.958409761806145	52.13375147104264	18.545820923979146
29	17.730748242966936	15.353083108156973	50.95642426513612	15.959744383739965
30	19.81436921033773	16.88674779590146	49.86883887891758	13.43004411484323
31	18.54499911244354	14.128748282413891	47.976864361649675	19.349388243492896
32	20.819773442995864	16.86916102903953	48.32399755428887	13.987067973675732
33	21.74283215978646	15.722569574564604	42.41402207714509	20.12057618850384
34	18.466598291946905	18.289580087177768	48.911921526860084	14.331900094015241
35	19.068493060623393	21.036731688395363	43.172554124507734	16.722221126473507
36	19.15231783725502	21.343103028868597	38.2214683471069	21.28311078676949
37	20.527537260935024	21.83684739945958	41.27334766572651	16.362267673878886
38	23.46617094994839	21.69730380071399	35.215446112173986	19.621079137163633
39	22.308731583203485	20.193717415172614	39.005640914380386	18.49191008724351
40	24.08581684779393	19.937147853756993	39.17213993149379	16.804895366955286
41	19.88110030702879	22.52043023477512	35.79597378092477	21.80249567727132
42	19.182889226379494	21.311381103594275	41.040446276536294	18.465283393489937
43	21.78852488116605	19.164973734903324	36.209344983333665	22.837156400596964
44	23.641216807032077	19.9900725166499	35.240757907470595	21.127952768847425
45	19.832777788735264	19.208529746290342	35.672044601355665	25.286647863618732
46	22.711583597956647	21.186794474796685	35.419091010696704	20.68253091654997
47	17.90973879542152	20.543644767032866	42.42930777170733	19.11730866583828
48	19.29465559522166	22.726869292518888	35.96148662419545	22.01698848806401
49	18.785132443147077	20.038230672636306	40.72684299454974	20.44979388966687
50	19.08739472594229	19.536596911303526	37.96719985799097	23.408808504763222
51	18.831811338369395	19.749446099025004	36.67268232710729	24.746060235498312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	1161.0
2	2233.0
3	25848.5
4	49464.0
5	35362.5
6	21261.0
7	19570.0
8	17879.0
9	17332.5
10	16786.0
11	16070.5
12	15355.0
13	14458.5
14	13562.0
15	12620.5
16	11679.0
17	10925.5
18	10172.0
19	9342.5
20	8513.0
21	8028.5
22	7544.0
23	7241.5
24	6939.0
25	6858.0
26	7067.5
27	7358.0
28	7532.5
29	7707.0
30	7914.5
31	8122.0
32	8750.5
33	9379.0
34	9928.5
35	10478.0
36	10636.5
37	10795.0
38	11140.5
39	11486.0
40	12134.0
41	12782.0
42	13687.5
43	14593.0
44	16393.5
45	18194.0
46	46424.5
47	74655.0
48	53420.0
49	32185.0
50	30821.0
51	29457.0
52	27701.0
53	25945.0
54	26336.0
55	26727.0
56	26420.0
57	26113.0
58	25249.5
59	24386.0
60	22054.0
61	19722.0
62	17922.0
63	16122.0
64	13839.5
65	11557.0
66	9534.0
67	7511.0
68	6347.5
69	5184.0
70	4327.0
71	3470.0
72	2931.5
73	2393.0
74	2031.5
75	1422.0
76	1174.0
77	868.0
78	562.0
79	405.0
80	248.0
81	179.0
82	110.0
83	76.5
84	43.0
85	30.5
86	18.0
87	12.5
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	608412.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.547377600045344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.08301366637188	42.88235372142807
2	9.629999749745815	10.313224657759628
3	4.538178253313307	7.290226336394456
4	2.4022162471611317	5.145295218548039
5	1.2695949085383567	3.3991738983299205
6	0.6910991771052994	2.2203929157322846
7	0.365050642967674	1.3683253235490564
8	0.22070944434041456	0.9454729564793896
9	0.15062141902389647	0.7258843799214516
>10	0.5493623841898675	5.275327596487731
>50	0.04478941617713183	1.6668572405085964
>100	0.050077052030911694	4.778890105822062
>500	0.0018662255415246268	0.6607136601190509
>1k	0.002177263131778731	2.864036324529165
>5k	3.1103759025410445E-4	1.6270511080673071
>10k+	9.331127707623134E-4	8.83677455632379
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23821	3.915274517925353	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	15864	2.607443640164888	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	13372	2.1978527708197735	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	9769	1.6056553782634135	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	3466	0.5696797564808058	Illumina PCR Primer Index 5 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	2677	0.43999789616246887	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC	2651	0.4357244761773272	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT	2615	0.4298074331209772	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT	2131	0.3502560764744943	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	2002	0.3290533388559069	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA	1654	0.2718552559778571	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGT	833	0.13691380183165355	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	782	0.12853132416849108	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	683	0.11225945576352866	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.643623071208326E-4	0.0	0.0	0.03994004063036232	0.0
2	1.643623071208326E-4	0.0	0.0	0.535985483521035	0.0
3	1.643623071208326E-4	0.0	0.0	0.6373970270145888	0.0
4	1.643623071208326E-4	0.0	0.0	1.3206511377158898	0.0
5	1.643623071208326E-4	0.0	0.0	4.42003116309343	0.0
6	1.643623071208326E-4	0.0	0.0	4.936293169759965	0.0
7	1.643623071208326E-4	0.0	0.0	5.472114290973879	0.0
8	1.643623071208326E-4	0.0	0.0	6.131371504835539	0.0
9	1.643623071208326E-4	0.0	0.0	6.3391254610362715	0.0
10	1.643623071208326E-4	0.0	0.0	9.040748703181396	0.0
11	1.643623071208326E-4	0.0	0.0	9.285155453870075	0.0
12	1.643623071208326E-4	0.0	0.0	11.313386323741149	0.0
13	1.643623071208326E-4	0.0	0.0	11.490733253124528	0.0
14	1.643623071208326E-4	0.0	0.0	11.649507241803251	0.0
15	1.643623071208326E-4	0.0	0.0	12.031649605859188	0.0
16	1.643623071208326E-4	0.0	0.0	12.254360532007915	0.0
17	1.643623071208326E-4	0.0	0.0	12.39932808688849	0.0
18	1.643623071208326E-4	0.0	0.0	12.489069906576464	0.0
19	1.643623071208326E-4	0.0	0.0	13.108880166729124	0.0
20	1.643623071208326E-4	0.0	0.0	13.231330085534145	0.0
21	1.643623071208326E-4	0.0	0.0	13.31729157215834	0.0
22	1.643623071208326E-4	0.0	0.0	13.513211442246373	0.0
23	1.643623071208326E-4	0.0	0.0	13.599337291177688	0.0
24	1.643623071208326E-4	0.0	0.0	13.695489240843376	0.0
25	1.643623071208326E-4	0.0	0.0	13.767973018283662	0.0
26	1.643623071208326E-4	0.0	0.0	13.873493619455237	0.0
27	1.643623071208326E-4	0.0	0.0	14.019611710485657	0.0
28	1.643623071208326E-4	0.0	0.0	14.107545544795304	0.0
29	1.643623071208326E-4	0.0	0.0	14.205998566760682	0.0
30	1.643623071208326E-4	0.0	0.0	14.34176183244249	0.0
31	1.643623071208326E-4	0.0	0.0	14.439886129793626	0.0
32	1.643623071208326E-4	0.0	0.0	14.549187064028981	0.0
33	1.643623071208326E-4	0.0	0.0	14.65963853441418	0.0
34	1.643623071208326E-4	0.0	0.0	14.824165203842133	0.0
35	1.643623071208326E-4	0.0	0.0	14.974063627936333	0.0
36	1.643623071208326E-4	0.0	0.0	15.105389111325877	0.0
37	1.643623071208326E-4	0.0	0.0	15.241316739314806	0.0
38	1.643623071208326E-4	0.0	0.0	15.44315365245919	0.0
39	1.643623071208326E-4	0.0	0.0	15.826117828050728	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18025	0.0	44.21359	1
TACGGCT	1205	0.0	43.319504	7
ACGGCTG	1215	0.0	43.148148	8
GATACCT	1605	0.0	42.61682	5
CCGATGA	390	0.0	42.115387	18
TGATACC	1650	0.0	41.863636	4
ATACCTG	1655	0.0	41.465256	6
CGTTCAT	110	0.0	40.909092	17
CGATGAA	415	0.0	40.662647	19
AATCTGT	1850	0.0	40.62162	2
AATGATC	255	0.0	40.588234	2
TACCTGT	1710	0.0	40.526314	7
GAATCTG	1850	0.0	40.5	1
CGACCAC	1195	0.0	40.481174	12
ATACGGC	3210	0.0	39.95327	6
ATGATAC	4915	0.0	39.918617	3
GAATGAT	5090	0.0	39.651276	1
AATGATA	4870	0.0	39.640656	2
CGGCTGT	1340	0.0	39.626865	9
TTCGTTG	115	0.0	39.130436	1
>>END_MODULE