SRR2933301.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933301.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466454
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35592	7.630334395245833	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	6012	1.2888730721571686	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	4533	0.9718000060027355	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	3841	0.8234466849893023	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	1451	0.31107033062209777	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT	1349	0.2892032226114472	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1328	0.2847011709621956	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC	819	0.1755800143208119	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	716	0.15349852289829222	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT	530	0.11362320829063531	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA	525	0.11255129123128968	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTGCTGTTCTCGTATGCCG	512	0.1097643068769911	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGGC	20	7.028971E-4	45.000004	4
CCTATAC	25	3.887229E-5	45.0	18
CGTTTTT	15410	0.0	44.43057	1
TACGGCT	515	0.0	43.252426	7
CCGATGA	90	0.0	42.5	18
ACGGCTG	535	0.0	41.635517	8
GAATGAT	2315	0.0	41.112312	1
CGACCAC	540	0.0	40.833336	12
AGACACG	215	0.0	40.813953	24
TGATACC	850	0.0	40.764706	4
GCGAGAC	210	0.0	40.714287	21
TCAAGCG	210	0.0	40.714287	17
ATACGGC	1400	0.0	40.5	6
CGTTTTC	50	1.0786607E-9	40.5	1
ATGATAC	2240	0.0	40.479908	3
AATGATA	2220	0.0	40.43919	2
GATACCT	855	0.0	40.263157	5
CTACGGC	45	1.924127E-8	40.0	6
TCAGACG	45	1.924127E-8	40.0	22
ACACGAC	215	0.0	39.76744	26