##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933297.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	528561
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.896549310297203	31.0	28.0	31.0	16.0	33.0
2	29.260942445621225	31.0	30.0	33.0	16.0	34.0
3	29.397344866533853	31.0	30.0	33.0	16.0	34.0
4	32.5435626162354	35.0	32.0	37.0	19.0	37.0
5	33.862776481806264	35.0	33.0	37.0	30.0	37.0
6	33.195230068052695	35.0	33.0	37.0	26.0	37.0
7	32.53734384489208	35.0	32.0	37.0	26.0	37.0
8	33.36198092556961	35.0	33.0	37.0	28.0	37.0
9	33.37672283804518	35.0	32.0	38.0	25.0	39.0
10	32.25282417734188	35.0	28.0	39.0	15.0	39.0
11	33.13392210170633	37.0	31.0	39.0	17.0	39.0
12	33.785746583648816	37.0	31.0	39.0	25.0	39.0
13	34.10436638344486	37.0	32.0	39.0	27.0	39.0
14	34.20867033322549	37.0	32.0	40.0	23.0	41.0
15	34.906029767614335	37.0	32.0	40.0	27.0	41.0
16	34.99157902304559	37.0	32.0	40.0	26.0	41.0
17	33.063460981797746	36.0	30.0	40.0	19.0	41.0
18	33.62165956247245	36.0	32.0	39.0	24.0	39.0
19	33.32040767290814	37.0	32.0	37.0	24.0	39.0
20	32.73180200582336	35.0	31.0	36.0	25.0	39.0
21	32.923352271544815	35.0	32.0	37.0	25.0	39.0
22	32.84586452651634	34.0	30.0	37.0	25.0	39.0
23	33.92421877512719	35.0	33.0	38.0	27.0	40.0
24	32.05891656781336	35.0	31.0	37.0	21.0	39.0
25	31.22731151182172	34.0	28.0	37.0	18.0	39.0
26	30.140791696701044	33.0	25.0	36.0	18.0	39.0
27	32.18232900270735	34.0	30.0	37.0	22.0	39.0
28	32.28121635913357	35.0	31.0	37.0	21.0	39.0
29	32.155792803479635	35.0	30.0	38.0	21.0	40.0
30	31.17143905812196	34.0	27.0	37.0	18.0	40.0
31	31.526557956413736	35.0	30.0	37.0	18.0	39.0
32	31.08266406337206	35.0	30.0	37.0	15.0	40.0
33	30.416479838656276	35.0	25.0	38.0	12.0	40.0
34	29.017695213986656	34.0	21.0	38.0	7.0	40.0
35	28.226460143673105	34.0	18.0	38.0	7.0	40.0
36	27.554753377566637	33.0	15.0	38.0	7.0	40.0
37	27.508126781960833	34.0	15.0	38.0	7.0	40.0
38	27.471958771078455	33.0	15.0	38.0	7.0	40.0
39	27.03866157359321	33.0	15.0	38.0	7.0	40.0
40	26.962988188685884	33.0	15.0	38.0	7.0	40.0
41	26.65626673174903	33.0	14.0	37.0	7.0	40.0
42	26.958858107200495	33.0	15.0	38.0	7.0	40.0
43	26.940646396537012	33.0	13.0	38.0	7.0	40.0
44	26.917042687598972	33.0	12.0	38.0	7.0	40.0
45	27.091981814776346	33.0	12.0	38.0	7.0	40.0
46	26.775882064700195	33.0	10.0	38.0	7.0	40.0
47	26.813616592976025	33.0	10.0	38.0	7.0	40.0
48	26.540257415889556	33.0	10.0	37.0	7.0	40.0
49	26.266888779156993	32.0	10.0	37.0	7.0	39.0
50	26.41921178444872	33.0	10.0	37.0	7.0	39.0
51	25.065740756506816	30.0	10.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	10.0
10	13.0
11	22.0
12	48.0
13	77.0
14	139.0
15	296.0
16	586.0
17	1177.0
18	2170.0
19	3785.0
20	5874.0
21	8337.0
22	12198.0
23	17044.0
24	23103.0
25	31186.0
26	36154.0
27	32489.0
28	27754.0
29	26167.0
30	27960.0
31	31740.0
32	36404.0
33	41732.0
34	47270.0
35	50415.0
36	38618.0
37	21238.0
38	4471.0
39	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.400903585395064	2.3138294350131776	33.80877514610424	45.47649183348753
2	46.902249693034484	9.063476117231502	31.863493522980317	12.170780666753696
3	10.334474166652477	8.994231507810829	67.8006890406216	12.870605284915083
4	9.465700269221527	2.3919661117638267	73.5281263657364	14.614207253278241
5	14.701803576124611	2.942517514534746	68.35918654611294	13.99649236322771
6	11.625337472874465	8.13321452017837	70.08727469487911	10.15417331206805
7	48.524578998450515	0.8687738974309492	48.314007276359774	2.2926398277587636
8	50.4655091843704	5.420188019925798	41.53749519922961	2.5768075964742008
9	46.889384574344305	2.1762861807814047	41.54184663643364	9.392482608440652
10	24.193044889804582	12.899551801967984	50.355588096738124	12.55181521148931
11	18.099897646629245	16.4444595798782	52.79787952573118	12.657763247761375
12	13.617728133555069	14.1448196140086	57.21648021704212	15.020972035394212
13	13.835489186678549	14.712019993907987	60.5928171015266	10.859673717886867
14	13.29231630786229	16.39224233342982	56.4413568159588	13.874084542749088
15	11.561201072345481	15.822393252623632	60.15332194391944	12.463083731111452
16	12.13880706295016	14.81002192745965	55.73699156767147	17.31417944191872
17	12.75633276007878	16.053208617359207	57.428943868352	13.761514754210017
18	13.059419820985656	15.181786019021457	56.81671557303698	14.942078586955903
19	13.619620062774212	17.688592234387325	53.74743880081958	14.944348902018877
20	14.835941357761923	16.484379286402138	57.28704917691619	11.392630178919747
21	14.830454763026404	18.256171000130543	54.832838593842524	12.080535643000525
22	13.560591871136918	15.138650032824971	55.923724981600984	15.377033114437122
23	13.505158345015996	18.556230974286787	54.90265078202894	13.03595989866827
24	13.765676998492133	16.23918525960107	54.9230836175957	15.072054124311101
25	12.545761037988045	19.194567892825994	53.651707182330895	14.607963886855066
26	13.18239522023002	16.90060371461383	55.64958443774701	14.267416627409135
27	14.013141340356174	17.142581461742353	53.82519709172641	15.019080106175068
28	13.281153925469342	17.03209279534434	55.89326492117277	13.793488358013551
29	16.059451983782385	15.292274685419468	53.64678816636111	15.001485164437028
30	13.561727028668402	17.86094698625135	53.54954300449711	15.02778298058313
31	15.396519985394304	16.29424039987816	52.47265689296032	15.836582721767215
32	15.088324715595741	18.55528500967722	52.33624879626003	14.020141478467007
33	14.415365492346199	18.821290257888872	48.65814920132208	18.105195048442848
34	17.824243559399953	19.782011915370223	46.67086674953317	15.722877775696656
35	14.955322091489915	20.835059718745804	43.923596330414085	20.286021859350196
36	20.295859891289748	20.320833356982448	40.70353279943091	18.679773952296898
37	16.342862980810164	22.58471586061022	43.05974144895291	18.012679709626703
38	19.061565268720166	24.283668299401583	40.11873747779348	16.53602895408477
39	18.743721159903963	22.539687945194594	41.80539237666041	16.911198518241036
40	17.99849024048312	23.654223448192358	41.47279121993488	16.87449509138964
41	19.727145968015044	23.301189455900076	40.21560425381366	16.756060322271225
42	17.647348177409985	23.230809688947918	41.99628803487204	17.125554098770056
43	20.43775458272555	22.393820202398587	38.88028061094178	18.28814460393408
44	18.8676425237579	23.297405597461786	38.037047757969276	19.797904120811033
45	18.05581569582319	22.758394962927646	38.56735551809536	20.618433823153808
46	19.694415592523853	23.60314135927547	37.63974262194903	19.062700426251652
47	17.401964957687003	22.417469317637888	41.1341358897081	19.046429834967014
48	18.7283965332289	22.63996019380923	37.92447796943021	20.707165303531664
49	17.21750185882046	22.135950249829254	39.885462605073016	20.76108528627727
50	18.541663119299383	21.535451915672933	39.01082372706272	20.912061237964966
51	18.281333658745158	21.716887927788843	37.85807125383825	22.14370715962774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45.0
1	315.5
2	586.0
3	21050.0
4	41514.0
5	29673.0
6	17832.0
7	17237.0
8	16642.0
9	16771.5
10	16901.0
11	16775.5
12	16650.0
13	16277.0
14	15904.0
15	15197.0
16	14490.0
17	13888.5
18	13287.0
19	12624.0
20	11961.0
21	11050.5
22	10140.0
23	9670.5
24	9201.0
25	8832.5
26	8297.5
27	8131.0
28	8202.0
29	8273.0
30	8683.5
31	9094.0
32	9764.5
33	10435.0
34	11004.5
35	11574.0
36	12776.5
37	13979.0
38	14753.0
39	15527.0
40	16799.5
41	18072.0
42	19143.5
43	20215.0
44	21795.5
45	23376.0
46	35529.0
47	47682.0
48	40736.5
49	33791.0
50	30813.0
51	27835.0
52	24713.5
53	21592.0
54	19382.5
55	17173.0
56	15482.5
57	13792.0
58	12382.5
59	10973.0
60	9419.5
61	7866.0
62	6796.0
63	5726.0
64	4731.0
65	3736.0
66	3097.5
67	2459.0
68	1974.5
69	1490.0
70	1175.0
71	860.0
72	679.5
73	499.0
74	425.0
75	288.0
76	225.0
77	170.5
78	116.0
79	87.5
80	59.0
81	37.5
82	16.0
83	17.5
84	19.0
85	11.0
86	3.0
87	2.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	528561.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.27208030999748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.76991988005224	47.40405498622409
2	7.682213372430759	8.79952682448782
3	3.5372931329862087	6.077644091771662
4	1.8560614289098258	4.252019968672489
5	1.2111058403605748	3.468127547651892
6	0.8099577708542307	2.783277990004202
7	0.5676904031168012	2.2758967250964197
8	0.3917432694808433	1.7948761592486273
9	0.2668181179842528	1.375310581322086
>10	0.8451337682026957	7.272758894648142
>50	0.02885091550645215	1.182008043343403
>100	0.02885091525156884	3.2117110407621774
>500	0.001677378793695862	0.5944624288177898
>1k	0.002012854552435035	2.8622193930506192
>5k	3.3547575873917247E-4	1.3702993026271744
>10k+	3.3547575873917247E-4	5.275806022271393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27459	5.195048442847656	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCG	7132	1.3493239190935389	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTC	4856	0.9187208288163523	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGC	4577	0.8659360036022332	No Hit
GAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCT	1702	0.3220063530983179	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGTATTGT	1410	0.26676201989931153	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	1230	0.23270729395471856	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTC	1122	0.2122744583879628	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTA	713	0.134894553324971	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTT	679	0.12846199397988123	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCT	586	0.11086705224184154	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGCGTATTGTCGTATGCCG	566	0.10708319380355341	No Hit
GAATCTTTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTC	550	0.10405610705292294	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05902819163729447	0.0
2	0.0	0.0	0.0	0.42322456632252475	0.0
3	0.0	0.0	0.0	0.5552812258187797	0.0
4	0.0	0.0	0.0	1.0978865258692942	0.0
5	0.0	0.0	0.0	2.3541275273809457	0.0
6	0.0	0.0	0.0	2.7758385503281553	0.0
7	0.0	0.0	0.0	3.131899629371066	0.0
8	0.0	0.0	0.0	3.602422426172192	0.0
9	0.0	0.0	0.0	3.8042912738548624	0.0
10	0.0	0.0	0.0	5.626975883578243	0.0
11	0.0	0.0	0.0	6.0095239716891715	0.0
12	0.0	0.0	0.0	7.346361157936359	0.0
13	0.0	0.0	0.0	7.539527131210967	0.0
14	0.0	0.0	0.0	7.656637549875984	0.0
15	0.0	0.0	0.0	7.919048132571264	0.0
16	0.0	0.0	0.0	8.101051723452922	0.0
17	0.0	0.0	0.0	8.286271594007125	0.0
18	0.0	0.0	0.0	8.464112940606666	0.0
19	0.0	0.0	0.0	8.941257489674797	0.0
20	0.0	0.0	0.0	9.10585533174033	0.0
21	0.0	0.0	0.0	9.303751128062796	0.0
22	0.0	0.0	0.0	9.570323955040195	0.0
23	0.0	0.0	0.0	9.781084870052842	0.0
24	0.0	0.0	0.0	9.953439621916864	0.0
25	0.0	0.0	0.0	10.107442660355192	0.0
26	0.0	0.0	0.0	10.256337489901828	0.0
27	0.0	0.0	0.0	10.40542151237038	0.0
28	0.0	0.0	0.0	10.547316203806183	0.0
29	0.0	0.0	0.0	10.709265344964914	0.0
30	0.0	0.0	0.0	10.882755254360424	0.0
31	0.0	0.0	0.0	11.053218077005303	0.0
32	0.0	0.0	0.0	11.23257296698016	0.0
33	0.0	0.0	0.0	11.414198172017988	0.0
34	0.0	0.0	0.0	11.5916611327737	0.0
35	0.0	0.0	0.0	11.771205215670472	0.0
36	0.0	0.0	0.0	11.953965578239787	0.0
37	0.0	0.0	0.0	12.132942082370814	0.0
38	0.0	0.0	0.0	12.324783705192022	0.0
39	0.0	0.0	0.0	12.541409600784016	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	30	2.1632677E-6	45.000004	4
AGCGGGT	40	6.8030204E-9	45.0	4
GTAGATT	25	3.8879363E-5	45.0	9
GCGATAT	25	3.8879363E-5	45.0	9
CCGGTCT	25	3.8879363E-5	45.0	18
CGCGTTG	20	7.029826E-4	45.0	1
GGCGAAT	20	7.029826E-4	45.0	8
AGGGCGA	70	0.0	45.0	6
CTAGCGG	25	3.8879363E-5	45.0	2
CGTTTTT	19475	0.0	44.468548	1
ACGGCTG	755	0.0	43.211918	8
GCGATCT	85	0.0	42.352943	9
TACGGCT	770	0.0	42.077923	7
CCGGTGT	75	0.0	42.0	18
CACCGGT	135	0.0	41.666664	16
CGGCTGT	740	0.0	41.655403	9
ACCGGTG	105	0.0	40.714287	17
TGATACC	875	0.0	40.62857	4
GACCGAT	100	0.0	40.5	9
GATACCT	880	0.0	40.397728	5
>>END_MODULE