##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933295.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453309
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.554577561883836	31.0	28.0	31.0	16.0	33.0
2	28.82693924012098	31.0	30.0	33.0	16.0	34.0
3	29.02545724880821	31.0	30.0	33.0	16.0	34.0
4	32.04526713566243	35.0	30.0	37.0	19.0	37.0
5	33.67193239048861	35.0	33.0	37.0	28.0	37.0
6	32.706370268404115	35.0	33.0	37.0	17.0	37.0
7	32.13589847102087	35.0	32.0	35.0	25.0	37.0
8	32.86173007815861	35.0	32.0	35.0	27.0	37.0
9	33.03075165064007	35.0	31.0	37.0	25.0	39.0
10	31.470153912673254	35.0	27.0	39.0	15.0	39.0
11	32.324017392110015	35.0	28.0	39.0	17.0	39.0
12	33.16591331740601	35.0	31.0	39.0	23.0	39.0
13	33.370674308253314	35.0	31.0	39.0	25.0	39.0
14	33.58031276678822	36.0	32.0	40.0	20.0	41.0
15	34.128157614342534	37.0	32.0	40.0	25.0	41.0
16	34.29278924530508	37.0	32.0	40.0	25.0	41.0
17	32.26278984092529	35.0	27.0	39.0	18.0	41.0
18	32.960841280451085	36.0	30.0	39.0	22.0	39.0
19	32.75680165185337	36.0	30.0	37.0	20.0	39.0
20	32.466997125581	34.0	30.0	37.0	24.0	39.0
21	32.88946391975452	35.0	31.0	37.0	24.0	39.0
22	33.09618604528037	35.0	31.0	38.0	25.0	39.0
23	34.120079239547415	35.0	33.0	38.0	27.0	40.0
24	32.441947986914	35.0	31.0	37.0	22.0	39.0
25	31.61161591761911	35.0	30.0	37.0	18.0	39.0
26	30.797054547780874	33.0	25.0	37.0	19.0	39.0
27	32.481095676459105	35.0	30.0	37.0	22.0	39.0
28	32.51666302676541	35.0	31.0	38.0	21.0	39.0
29	32.73614245470529	35.0	31.0	38.0	22.0	40.0
30	31.653704206181654	35.0	30.0	38.0	18.0	40.0
31	31.96460912975476	35.0	30.0	37.0	21.0	40.0
32	31.845421114515705	35.0	30.0	37.0	18.0	40.0
33	31.80175332940665	35.0	30.0	38.0	16.0	40.0
34	30.934750909423816	35.0	29.0	38.0	12.0	40.0
35	30.50240343783159	35.0	28.0	38.0	10.0	40.0
36	29.713678748932846	35.0	25.0	38.0	8.0	40.0
37	29.90833846228511	35.0	25.0	38.0	8.0	40.0
38	29.77306208347948	35.0	24.0	38.0	8.0	40.0
39	29.42387201665972	34.0	23.0	38.0	7.0	40.0
40	29.358395708004917	34.0	23.0	38.0	8.0	40.0
41	28.72489846881487	33.0	22.0	38.0	7.0	40.0
42	29.1875210948823	34.0	23.0	38.0	7.0	40.0
43	28.957675669355783	34.0	22.0	38.0	7.0	40.0
44	29.049910767269125	34.0	22.0	38.0	7.0	40.0
45	29.292213479105865	35.0	23.0	38.0	7.0	40.0
46	29.06023043883973	34.0	23.0	38.0	7.0	40.0
47	29.152284644690486	34.0	23.0	38.0	7.0	40.0
48	28.896384144148914	34.0	23.0	38.0	7.0	40.0
49	28.739833093982252	34.0	22.0	37.0	7.0	40.0
50	28.804281406281365	34.0	22.0	38.0	7.0	40.0
51	27.358009657871342	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	14.0
10	18.0
11	17.0
12	40.0
13	54.0
14	95.0
15	212.0
16	441.0
17	855.0
18	1386.0
19	2298.0
20	3507.0
21	4942.0
22	7112.0
23	9825.0
24	13371.0
25	18216.0
26	23601.0
27	25918.0
28	25306.0
29	25657.0
30	28309.0
31	32616.0
32	36731.0
33	41148.0
34	45893.0
35	48943.0
36	35146.0
37	17967.0
38	3609.0
39	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.978806950667206	2.611243103490114	34.51751454306003	39.89243540278265
2	40.574751438864	11.592754611093095	34.49699873596156	13.335495214081345
3	13.066804321114295	11.382081538200211	61.90611701951649	13.644997121169004
4	11.726658857423965	2.9785422305756115	69.18525773809918	16.109541173901246
5	18.853364923264262	3.3211341491124156	61.28909860602811	16.536402321595205
6	14.015825849475744	10.870730561272774	63.46862736014507	11.644816229106416
7	52.58796979543755	1.2958048483484774	43.23982096097805	2.876404395235921
8	53.571846135858756	8.078154195041352	34.507808139701616	3.8421915293982694
9	49.96503488790207	2.5516810828816547	34.97305370067658	12.510230328539693
10	29.026116843036426	16.298374839237695	41.05235060411331	13.623157713612569
11	22.290314112448684	16.341171254045253	47.63792468272194	13.730589950784122
12	18.202153497945112	14.548133833654306	50.45785545841799	16.791857209982595
13	16.575227935028867	15.30148309431315	55.276202325566004	12.847086645091979
14	14.814398125781752	17.948022210015687	49.308529060751056	17.92905060345151
15	11.909095120546912	15.623779805827812	56.5689187728459	15.89820630077938
16	13.0923939299683	15.034336401880394	49.96635848836004	21.90691117979127
17	13.89537820780086	15.928207911159936	52.79555446726184	17.380859413777358
18	14.041636058406077	15.014482395010909	52.26920268514414	18.67467886143889
19	14.704539287770594	17.762497545824154	49.117268794575004	18.415694371830252
20	16.97627887379249	16.399630274272077	53.23322501869585	13.390865833239578
21	17.327032995153417	19.621494389037057	48.135598454917066	14.915874160892459
22	15.081765418290836	15.781508860402067	50.22071037636579	18.916015344941307
23	15.20486026088165	20.115417959934614	48.699672850086806	15.98004892909693
24	15.852321484903234	16.072480361078203	49.21234742747221	18.862850726546352
25	14.075829070236859	20.621033334877534	47.28827356174265	18.014864033142956
26	14.646080267543773	17.660800910637114	49.94584268126157	17.747276140557545
27	15.430092938812157	18.620631842738618	48.03257821927207	17.916696999177162
28	13.082246326457229	18.8752043308207	51.88293195149446	16.159617391227616
29	16.854066431506986	17.075328308063593	48.511500984979335	17.55910427545008
30	16.633245755103033	19.99607331864137	48.51083918475036	14.859841741505242
31	17.160921137678713	19.245591858974784	45.828783456759076	17.764703546587427
32	19.321478285231485	20.364696046184832	45.69907061187843	14.61475505670525
33	18.173034287869864	18.299438131605594	43.66227010714546	19.865257473379085
34	19.247356659585403	22.74122066846235	41.834377874694745	16.1770447972575
35	20.035119532151356	20.795086795100033	40.71902388878226	18.450769783966344
36	18.12957607283332	25.25628213867362	39.61359690630453	17.000544882188528
37	19.40662991469395	22.487309980609254	42.52926811512677	15.57679198957003
38	20.685227957088873	23.72664120941786	38.689061986415446	16.89906884707782
39	20.882003225173115	22.118025452836807	40.49952681283628	16.5004445091538
40	23.411844900498334	21.341071984010906	39.01532949930401	16.23175361618675
41	19.92349589352958	22.67173164441915	40.21032011277076	17.19445234928051
42	19.213825447983606	22.103024647646528	41.031172996785855	17.65197690758401
43	19.0388895874558	21.531891050034304	39.69367473401146	19.73554462849844
44	20.724494770675193	21.698223507585336	38.40912048955569	19.168161232183785
45	18.207227299700644	22.54731320137037	38.09410358055984	21.15135591836915
46	20.10372615588925	23.872678459946748	38.25955363780556	17.764041746358444
47	17.30276698675738	21.233198546686697	43.32409019013521	18.13994427642072
48	20.035340132227685	22.04809522864095	37.63900562309595	20.277559016035422
49	18.1006774628344	20.61662133335098	41.28927508608918	19.993426117725438
50	18.92329514746012	20.09181375176756	40.0014118404885	20.983479260283826
51	18.804171106243203	20.466613281448197	38.00630475018144	22.722910862127158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84.0
1	508.0
2	932.0
3	21926.0
4	42920.0
5	29169.0
6	15418.0
7	14705.0
8	13992.0
9	13588.0
10	13184.0
11	12404.0
12	11624.0
13	10596.0
14	9568.0
15	8744.5
16	7921.0
17	7125.5
18	6330.0
19	5843.0
20	5356.0
21	4857.0
22	4358.0
23	4213.5
24	4069.0
25	3986.0
26	4040.5
27	4178.0
28	4605.0
29	5032.0
30	5480.0
31	5928.0
32	6515.0
33	7102.0
34	7878.5
35	8655.0
36	9244.0
37	9833.0
38	10351.5
39	10870.0
40	12328.0
41	13786.0
42	14878.5
43	15971.0
44	18043.5
45	20116.0
46	33572.5
47	47029.0
48	39499.0
49	31969.0
50	29803.0
51	27637.0
52	23857.0
53	20077.0
54	18325.0
55	16573.0
56	15388.0
57	14203.0
58	13698.5
59	13194.0
60	12358.5
61	11523.0
62	9996.0
63	8469.0
64	7551.5
65	6634.0
66	5651.0
67	4668.0
68	4048.5
69	3429.0
70	2987.0
71	2545.0
72	2113.5
73	1682.0
74	1362.0
75	865.0
76	688.0
77	546.5
78	405.0
79	316.5
80	228.0
81	168.0
82	108.0
83	76.0
84	44.0
85	30.5
86	17.0
87	13.0
88	9.0
89	6.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	453309.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.97976394364743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.14724350632937	34.87689370579714
2	6.652382649086933	5.718356718411446
3	2.712308900778688	3.4972318889316547
4	1.745418294348569	3.0007066509610087
5	1.2957190515302501	2.7844849486028442
6	0.9896668405508353	2.5521388313838154
7	0.7876077050567047	2.369583527047454
8	0.6957381209736289	2.3922128164834704
9	0.5848621711117713	2.2623514248557752
>10	3.281349097559377	22.138115664921347
>50	0.06777449992646895	1.9579870886383726
>100	0.03309917438269396	2.990836003807535
>500	0.0021015348814408864	0.6085544001707291
>1k	0.0031523023221613294	2.6033483041069894
>5k	0.0010507674407204432	3.5192283215810067
>10k+	5.253837203602216E-4	6.727969704299397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29795	6.572779274181628	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	8972	1.979223884811464	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	6613	1.4588283047545936	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	4162	0.9181375176755812	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1924	0.4244345468543532	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGATGGGTT	1804	0.39796253769503803	No Hit
GAACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	1445	0.31876711029342014	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTA	1192	0.2629552909825307	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTC	1002	0.22104127648028166	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGATGGGTTCGTATGCCG	735	0.1621410561008054	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCT	714	0.15750845449792525	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	660	0.1455960503762334	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGT	586	0.12927164472798908	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	489	0.10787343732420931	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	489	0.10787343732420931	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	486	0.10721163709522642	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1780242615963945	0.0
2	0.0	0.0	0.0	0.9106371150804419	0.0
3	0.0	0.0	0.0	1.1113831845385818	0.0
4	0.0	0.0	0.0	1.7449466037515249	0.0
5	0.0	0.0	0.0	3.074723863854457	0.0
6	0.0	0.0	0.0	3.379813769415564	0.0
7	0.0	0.0	0.0	3.747995296806373	0.0
8	0.0	0.0	0.0	4.252728271443982	0.0
9	0.0	0.0	0.0	4.417516528460719	0.0
10	0.0	0.0	0.0	7.154942875610235	0.0
11	0.0	0.0	0.0	7.925498942222634	0.0
12	0.0	0.0	0.0	10.141426708933642	0.0
13	0.0	0.0	0.0	10.389822394878548	0.0
14	2.206000763276264E-4	0.0	0.0	10.566964256169632	0.0
15	2.206000763276264E-4	0.0	0.0	11.068829429814983	0.0
16	2.206000763276264E-4	0.0	0.0	11.42311315239715	0.0
17	2.206000763276264E-4	0.0	0.0	11.75776346818616	0.0
18	2.206000763276264E-4	0.0	0.0	12.057338371839077	0.0
19	2.206000763276264E-4	0.0	0.0	12.82193823639063	0.0
20	2.206000763276264E-4	0.0	0.0	13.138278745844445	0.0
21	2.206000763276264E-4	0.0	0.0	13.473590861862439	0.0
22	2.206000763276264E-4	0.0	0.0	13.953616627951353	0.0
23	2.206000763276264E-4	0.0	0.0	14.303708949083296	0.0
24	2.206000763276264E-4	0.0	0.0	14.581003245027123	0.0
25	2.206000763276264E-4	0.0	0.0	14.825869329750788	0.0
26	2.206000763276264E-4	0.0	0.0	15.041836804475535	0.0
27	2.206000763276264E-4	0.0	0.0	15.2635398811848	0.0
28	2.206000763276264E-4	0.0	0.0	15.474654154230338	0.0
29	2.206000763276264E-4	0.0	0.0	15.693930630099999	0.0
30	4.412001526552528E-4	0.0	0.0	15.957106521158856	0.0
31	4.412001526552528E-4	0.0	0.0	16.191604402295123	0.0
32	4.412001526552528E-4	0.0	0.0	16.42610228343139	0.0
33	6.618002289828792E-4	0.0	0.0	16.659497164186018	0.0
34	6.618002289828792E-4	0.0	0.0	16.90193664807008	0.0
35	6.618002289828792E-4	0.0	0.0	17.149449933709676	0.0
36	6.618002289828792E-4	0.0	0.0	17.389462816754136	0.0
37	6.618002289828792E-4	0.0	0.0	17.614474894608314	0.0
38	6.618002289828792E-4	0.0	0.0	17.825147967501195	0.0
39	6.618002289828792E-4	0.0	0.0	18.10376586390299	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCAA	20	7.0287596E-4	45.000004	44
CGCATGT	20	7.0287596E-4	45.000004	38
GAGTAGA	20	7.0287596E-4	45.000004	9
CGCGCAT	20	7.0287596E-4	45.000004	36
ATCCGTC	20	7.0287596E-4	45.000004	6
TACGCGC	20	7.0287596E-4	45.000004	34
GTACTAT	20	7.0287596E-4	45.000004	40
GCGATCG	20	7.0287596E-4	45.000004	9
TTACACG	40	6.7993824E-9	45.000004	34
TGGCTAA	20	7.0287596E-4	45.000004	43
GACTAGT	20	7.0287596E-4	45.000004	3
GCGAGAC	25	3.887055E-5	45.0	21
ACCGCTC	25	3.887055E-5	45.0	30
CGAGACA	25	3.887055E-5	45.0	22
ACGAGTG	25	3.887055E-5	45.0	30
TGCGTTA	25	3.887055E-5	45.0	30
CGACCAA	30	2.162582E-6	44.999996	29
GTCAAGC	30	2.162582E-6	44.999996	16
CGTTTTT	13265	0.0	44.03317	1
TGATACC	1100	0.0	42.545456	4
>>END_MODULE