##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933292.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563646
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.516448267174788	31.0	28.0	33.0	25.0	34.0
2	28.84706358246133	31.0	28.0	33.0	16.0	34.0
3	28.81461945973182	31.0	28.0	34.0	16.0	34.0
4	33.252028755637404	35.0	32.0	37.0	28.0	37.0
5	34.30725845654897	35.0	33.0	37.0	30.0	37.0
6	33.88147880052373	35.0	33.0	37.0	28.0	37.0
7	33.760706187926466	35.0	33.0	37.0	29.0	37.0
8	33.70485907821576	35.0	33.0	37.0	28.0	37.0
9	34.861343822186264	37.0	34.0	39.0	28.0	39.0
10	34.60955457858301	37.0	33.0	39.0	27.0	39.0
11	34.805438165089434	37.0	34.0	39.0	27.0	39.0
12	34.957844107826546	37.0	34.0	39.0	27.0	39.0
13	35.0854916028855	37.0	34.0	39.0	27.0	39.0
14	35.97264772570017	38.0	34.0	40.0	27.0	41.0
15	36.23753739048978	38.0	34.0	40.0	27.0	41.0
16	36.2315886212268	38.0	34.0	41.0	30.0	41.0
17	35.46386739194459	38.0	33.0	40.0	27.0	41.0
18	35.2009967248947	37.0	33.0	39.0	27.0	40.0
19	34.57957122023398	37.0	33.0	37.0	27.0	40.0
20	33.73368923047445	35.0	32.0	37.0	27.0	39.0
21	33.89625225762269	35.0	33.0	38.0	27.0	40.0
22	34.343359129666496	35.0	33.0	38.0	28.0	40.0
23	34.6972958204263	35.0	33.0	38.0	29.0	40.0
24	34.204796982503204	35.0	33.0	38.0	27.0	40.0
25	33.37868271929545	35.0	33.0	38.0	24.0	40.0
26	33.17282478718912	35.0	32.0	38.0	23.0	40.0
27	34.076397242240695	35.0	33.0	38.0	25.0	40.0
28	33.827010215631795	35.0	33.0	38.0	24.0	40.0
29	33.875400517345994	35.0	33.0	39.0	24.0	40.0
30	33.13259918459459	35.0	33.0	38.0	21.0	40.0
31	32.93972103057593	35.0	33.0	38.0	21.0	40.0
32	32.20341136103157	35.0	31.0	38.0	16.0	40.0
33	31.38091461662107	35.0	30.0	39.0	12.0	40.0
34	30.646350723681177	35.0	25.0	39.0	8.0	40.0
35	30.008705818900516	35.0	23.0	39.0	7.0	40.0
36	29.328793249663796	35.0	20.0	39.0	7.0	40.0
37	29.24474758979927	35.0	18.0	39.0	7.0	40.0
38	28.974743012458173	35.0	18.0	39.0	7.0	40.0
39	28.610074408405275	35.0	18.0	39.0	7.0	40.0
40	28.42834687019867	35.0	18.0	38.0	7.0	40.0
41	28.063089243958085	34.0	18.0	38.0	7.0	40.0
42	28.21567969966965	34.0	17.0	39.0	7.0	40.0
43	28.054048108209763	34.0	17.0	38.0	7.0	40.0
44	28.054885513247676	34.0	15.0	39.0	7.0	40.0
45	28.17879129808426	35.0	17.0	39.0	7.0	40.0
46	27.94558464000454	34.0	15.0	38.0	7.0	40.0
47	27.832982049016582	34.0	15.0	38.0	7.0	40.0
48	27.652785613665316	34.0	15.0	38.0	7.0	40.0
49	27.789594887571276	34.0	15.0	38.0	7.0	40.0
50	27.795240274924332	34.0	15.0	38.0	7.0	40.0
51	26.67146045567608	33.0	15.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	21.0
10	25.0
11	32.0
12	48.0
13	84.0
14	117.0
15	192.0
16	356.0
17	646.0
18	1234.0
19	2354.0
20	3709.0
21	5713.0
22	8262.0
23	12213.0
24	17937.0
25	25829.0
26	33193.0
27	34256.0
28	30104.0
29	26940.0
30	26664.0
31	28905.0
32	32475.0
33	37637.0
34	41323.0
35	47301.0
36	50645.0
37	51386.0
38	38654.0
39	5383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.75728737540939	2.3993783332091416	29.953552407007233	43.88978188437424
2	42.87850885130028	14.035760033780068	30.04367989837593	13.042051216543719
3	10.873846350368849	14.284852549295124	61.28101680842231	13.56028429191372
4	9.421516341817382	2.4813446737846094	72.67593489530663	15.42120408909138
5	17.93856427615915	2.9218694002973495	63.06138959559724	16.078176727946264
6	12.333273011783993	11.532770568761244	65.85889015445865	10.275066264996115
7	48.78381111548738	1.0229825102990175	47.88342328340838	2.3097830908052215
8	48.2013533317011	8.36588922834545	40.570677340032574	2.8620800999208726
9	45.43472321279668	2.13786667518265	39.70311152744808	12.724298584572585
10	29.157485372024283	13.297885552279268	45.41751382960227	12.12711524609418
11	23.417357703239265	14.32778729912037	49.84138980849682	12.41346518914354
12	19.152446748491077	13.133598038485147	52.55585952885322	15.158095684170561
13	16.497411495867976	14.19295089471051	57.89289731498138	11.416740294440126
14	14.890551871209945	18.084932741472485	52.67455104799821	14.34996433931936
15	13.604283539668515	13.2576120472779	60.15087483988177	12.987229573171813
16	15.63179726282099	15.557282407752385	51.42820138881496	17.38271894061166
17	15.757585434829663	15.425816913452769	55.73374068120771	13.082856970509859
18	16.19420700226738	15.45455835755066	54.08004314764941	14.271191492532548
19	14.601540683336706	20.0478669235655	49.78781007937606	15.562782313721732
20	16.2662380288337	19.360556093718397	54.24113716765488	10.132068709793026
21	16.925871912512463	22.01949450541652	50.77584157432147	10.278792007749544
22	14.764231450236496	14.56960574545016	53.63100243770026	17.035160366613088
23	14.384737938351378	19.451038417730278	54.685565053242634	11.478658590675709
24	16.709246583848728	15.303577067875937	52.82606458663771	15.161111761637624
25	14.297094275484968	21.23087895594043	51.0781589863141	13.393867782260497
26	13.021293506917461	13.746748845906827	55.97449462960794	17.257463017567765
27	15.519847563896489	15.42670399506073	51.00967628617963	18.04377215486316
28	11.039553194735703	17.40400889920269	55.19936272057284	16.357075185488764
29	16.35459135698648	14.302949014097502	53.655485890079945	15.686973738836077
30	16.422719224477774	17.560135262203584	50.015967468943266	16.00117804437537
31	15.688393069408813	15.36123737239331	47.8259403952126	21.12442916298528
32	17.687165348463395	18.172221571695708	49.685263445495934	14.45534963434496
33	15.292577255937237	16.743133101272782	44.36578987520536	23.59849976758462
34	16.431767456878962	18.163528171937706	47.51546183242674	17.889242538756594
35	18.174173151233223	20.761790201651394	44.063472463212726	17.00056418390266
36	15.389269151204834	22.246055148089404	42.082619232638926	20.282056468066838
37	17.74003541229779	22.57374309406968	43.125117538313056	16.561103955319474
38	18.00225673561065	21.84491684497007	37.28741089265248	22.8654155267668
39	19.201236236928853	20.635469780677944	38.84299720037044	21.32029678202276
40	17.97564428737186	21.13294514642169	41.70259347178903	19.188817094417416
41	16.623022251555053	22.884221656855544	40.75554514713136	19.73721094445805
42	17.277511061907653	21.569744130181	43.578593656302004	17.574151151609342
43	19.741646352497845	19.38770079092196	39.712337176170855	21.158315680409334
44	20.627486046206307	20.367038886109366	38.31340948041856	20.692065587265766
45	17.70792305808965	19.8436607374132	39.874495694105875	22.573920510391275
46	22.18484651714019	22.258474290600837	37.344184115561895	18.212495076697074
47	18.606536726952733	20.255798852471234	42.699850615457215	18.437813805118815
48	20.78751556828222	21.216863066534668	35.62342321244185	22.37219815274126
49	18.18517296317192	19.502311734670343	40.81018937418167	21.50232592797607
50	19.024174747980116	18.74474404147284	40.27102117286382	21.960060037683228
51	19.481731441365678	18.713696185194255	38.29016794229002	23.514404431150048
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	970.5
2	1866.0
3	22489.5
4	43113.0
5	31961.0
6	20809.0
7	19831.0
8	18853.0
9	18345.0
10	17837.0
11	17165.0
12	16493.0
13	15853.0
14	15213.0
15	14375.0
16	13537.0
17	12628.0
18	11719.0
19	10688.5
20	9658.0
21	8840.5
22	8023.0
23	7419.0
24	6815.0
25	6637.5
26	6306.5
27	6153.0
28	6236.5
29	6320.0
30	6751.0
31	7182.0
32	7595.0
33	8008.0
34	8732.0
35	9456.0
36	10179.0
37	10902.0
38	12087.0
39	13272.0
40	14761.5
41	16251.0
42	17898.5
43	19546.0
44	21346.0
45	23146.0
46	48666.5
47	74187.0
48	52555.0
49	30923.0
50	28478.5
51	26034.0
52	23617.0
53	21200.0
54	20628.0
55	20056.0
56	19096.0
57	18136.0
58	17534.5
59	16933.0
60	15492.5
61	14052.0
62	12356.0
63	10660.0
64	9044.0
65	7428.0
66	6098.5
67	4769.0
68	3919.5
69	3070.0
70	2529.0
71	1988.0
72	1657.0
73	1326.0
74	1139.5
75	779.0
76	605.0
77	482.0
78	359.0
79	243.0
80	127.0
81	104.5
82	82.0
83	56.5
84	31.0
85	19.5
86	8.0
87	8.0
88	8.0
89	5.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	563646.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.06389213640759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.11066621895625	48.2572875872245
2	9.540934618846219	11.079675971784079
3	3.57285071716435	6.22360855982752
4	1.5354321247655118	3.566126611006392
5	0.7193906133865794	2.088530948981122
6	0.3937412182667057	1.371728857625743
7	0.216006392398972	0.877952030831991
8	0.15327162785839796	0.7119637814033299
9	0.10192251392359866	0.53262160692582
>10	0.5601205206332338	6.025240341632932
>50	0.05139200743435135	2.0933079695229195
>100	0.039317980059155884	3.812355011571802
>500	0.0015479519708329033	0.5971634924764947
>1k	0.002167132759166065	2.685258353586357
>5k	3.095903941665807E-4	1.3199793874939496
>10k+	9.28771182499742E-4	8.757199488105032
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19831	3.518343073489389	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	14447	2.563133598038485	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	14438	2.561536851144158	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	7343	1.302768049449477	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	3416	0.6060541545580027	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC	2389	0.42384759228309965	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	2126	0.37718709970442443	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT	1957	0.34720374135538973	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTCCTTT	1932	0.3427683333155917	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT	1787	0.3170429666847631	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTA	1331	0.23614112403884707	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGT	831	0.1474329632428865	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	688	0.12206242925524176	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	642	0.11390127846201338	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	629	0.11159486628131841	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04435408039798029	0.0
2	0.0	0.0	0.0	0.4811530641572902	0.0
3	0.0	0.0	0.0	0.5810384532135419	0.0
4	0.0	0.0	0.0	1.3183806857495661	0.0
5	0.0	0.0	0.0	4.380586396426126	0.0
6	0.0	0.0	0.0	4.942109054264556	0.0
7	0.0	0.0	0.0	5.453955142057248	0.0
8	0.0	0.0	0.0	6.1174921848110335	0.0
9	0.0	0.0	0.0	6.335359427725913	0.0
10	0.0	0.0	0.0	9.705382456364456	0.0
11	0.0	0.0	0.0	9.987651824017203	0.0
12	0.0	0.0	0.0	11.691735592907605	0.0
13	0.0	0.0	0.0	11.88157105701096	0.0
14	0.0	0.0	0.0	12.023326697962906	0.0
15	0.0	0.0	0.0	12.363788619097802	0.0
16	0.0	0.0	0.0	12.600284575779833	0.0
17	0.0	0.0	0.0	12.733701649616957	0.0
18	0.0	0.0	0.0	12.834296703959577	0.0
19	0.0	0.0	0.0	13.3433041306068	0.0
20	0.0	0.0	0.0	13.450818421491503	0.0
21	0.0	0.0	0.0	13.534204092639706	0.0
22	0.0	0.0	0.0	13.710733332623668	0.0
23	0.0	0.0	0.0	13.815586378684493	0.0
24	0.0	0.0	0.0	13.910858943379354	0.0
25	0.0	0.0	0.0	13.984131884196819	0.0
26	0.0	0.0	0.0	14.085259187504214	0.0
27	0.0	0.0	0.0	14.219208510306114	0.0
28	0.0	0.0	0.0	14.30472317731342	0.0
29	0.0	0.0	0.0	14.394673252360525	0.0
30	0.0	0.0	0.0	14.491365147628121	0.0
31	0.0	0.0	0.0	14.58362163485592	0.0
32	0.0	0.0	0.0	14.703377651930467	0.0
33	0.0	0.0	0.0	14.804150122594677	0.0
34	0.0	0.0	0.0	14.935083367929517	0.0
35	0.0	0.0	0.0	15.05554905029043	0.0
36	0.0	0.0	0.0	15.185062965052532	0.0
37	0.0	0.0	0.0	15.32699602232607	0.0
38	0.0	0.0	0.0	15.50334784598844	0.0
39	0.0	0.0	0.0	15.732924566128386	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATTG	25	3.888266E-5	45.000004	1
CCGATGA	365	0.0	44.999996	18
CGTTTTT	17915	0.0	44.334354	1
TACGGCT	895	0.0	43.49162	7
TGATACC	1725	0.0	42.52174	4
GATACCT	1745	0.0	42.163326	5
CGATGAA	390	0.0	42.115383	19
ACGGCTG	925	0.0	41.837837	8
TACCTGT	1780	0.0	41.71348	7
ATACCTG	1780	0.0	41.71348	6
CGACCAC	1080	0.0	41.250004	12
ATGATCT	345	0.0	41.086956	3
ATGATAC	4350	0.0	41.01724	3
GCGACCA	1110	0.0	40.743244	11
GAATCTG	1730	0.0	40.70809	1
AATGATA	4300	0.0	40.604652	2
GAATGAT	4610	0.0	40.55857	1
CGGCTGT	960	0.0	40.54688	9
AATGATC	390	0.0	40.384617	2
ATACGGC	2620	0.0	40.276722	6
>>END_MODULE