##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933291.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411654
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.858835332585134	31.0	28.0	31.0	16.0	33.0
2	29.322095740597685	31.0	30.0	33.0	16.0	34.0
3	29.47462189119989	31.0	30.0	33.0	16.0	34.0
4	32.40432984982534	35.0	32.0	37.0	19.0	37.0
5	33.73473839680897	35.0	33.0	37.0	28.0	37.0
6	33.269704654880066	35.0	33.0	37.0	26.0	37.0
7	32.29020488079795	35.0	32.0	35.0	25.0	37.0
8	33.302807697726735	35.0	33.0	37.0	28.0	37.0
9	33.12061099855704	35.0	31.0	38.0	25.0	39.0
10	31.42720099889713	35.0	27.0	39.0	15.0	39.0
11	32.159532034184046	35.0	27.0	39.0	15.0	39.0
12	33.095796469850896	35.0	31.0	39.0	22.0	39.0
13	33.27866363499444	35.0	31.0	39.0	22.0	39.0
14	33.80346844680241	37.0	32.0	40.0	21.0	41.0
15	34.17180933502407	37.0	32.0	40.0	23.0	41.0
16	34.48722227890413	37.0	32.0	40.0	25.0	41.0
17	32.62642170366376	36.0	29.0	40.0	18.0	41.0
18	33.19460274891049	36.0	30.0	39.0	23.0	39.0
19	33.005849572699404	36.0	31.0	37.0	23.0	39.0
20	32.46028460794745	35.0	31.0	36.0	24.0	39.0
21	32.47803981013181	35.0	31.0	36.0	23.0	39.0
22	32.446637224465206	34.0	30.0	37.0	24.0	39.0
23	33.499081753122766	35.0	32.0	38.0	26.0	39.0
24	31.814693893415345	34.0	30.0	36.0	21.0	39.0
25	31.348557283543947	34.0	29.0	37.0	18.0	39.0
26	29.96313408833632	33.0	25.0	36.0	18.0	39.0
27	32.0778979434185	34.0	30.0	37.0	22.0	39.0
28	32.19703926112706	35.0	31.0	37.0	21.0	39.0
29	32.36510030268138	35.0	31.0	38.0	21.0	40.0
30	31.518583567753502	34.0	30.0	37.0	18.0	40.0
31	31.566842542523574	34.0	30.0	37.0	20.0	39.0
32	31.429644798787333	35.0	30.0	37.0	18.0	39.0
33	31.127939969003094	35.0	30.0	37.0	15.0	40.0
34	30.018943092985857	35.0	25.0	37.0	10.0	39.0
35	29.200466896957153	34.0	23.0	37.0	8.0	39.0
36	28.447130357047424	34.0	21.0	37.0	7.0	40.0
37	28.525358675003766	34.0	20.0	38.0	7.0	40.0
38	28.353644079736867	34.0	20.0	37.0	7.0	40.0
39	27.93687660025167	33.0	18.0	37.0	7.0	39.0
40	27.738807347918396	33.0	18.0	37.0	7.0	39.0
41	27.503184713375795	33.0	18.0	37.0	7.0	40.0
42	27.874644725910596	33.0	18.0	37.0	7.0	40.0
43	27.80303847405831	33.0	18.0	37.0	7.0	40.0
44	27.72563852167111	33.0	18.0	37.0	7.0	40.0
45	27.905401137848777	34.0	18.0	38.0	7.0	40.0
46	27.555495634683496	33.0	18.0	37.0	7.0	40.0
47	27.52122413483168	33.0	17.0	37.0	7.0	40.0
48	27.282914777944583	33.0	17.0	37.0	7.0	40.0
49	27.04823954097373	33.0	15.0	37.0	7.0	39.0
50	27.219820528890768	33.0	15.0	37.0	7.0	40.0
51	25.92330209350571	31.0	15.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	14.0
10	18.0
11	32.0
12	41.0
13	72.0
14	130.0
15	243.0
16	461.0
17	811.0
18	1493.0
19	2513.0
20	3796.0
21	5636.0
22	7926.0
23	10766.0
24	14669.0
25	20211.0
26	25302.0
27	27025.0
28	25322.0
29	24296.0
30	25789.0
31	28716.0
32	31482.0
33	33889.0
34	37219.0
35	39620.0
36	27424.0
37	14015.0
38	2682.0
39	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.155538389035453	2.5820227666924165	32.70197787462286	46.56046096964927
2	46.72200440175487	8.301631953047949	32.33395035636724	12.64241328882994
3	11.748458657027504	8.322523284117246	66.48593236067182	13.443085698183427
4	10.206386917168302	2.604128710033183	71.13789736040461	16.051587012393902
5	14.747336355288665	2.9843023510035125	66.59719084473853	15.67117044896928
6	12.933191466620025	7.313180486525092	68.23740325613258	11.516224790722305
7	49.67448391124585	1.0783327746116884	46.58718243962162	2.6600008745208354
8	51.03120581847862	5.105015376991357	40.9086271480418	2.9551516564882157
9	47.45392975654312	2.047350444791014	41.08037332322776	9.41834647543811
10	28.597317164414775	13.867714148289584	45.74715659267249	11.787812094623154
11	22.066346980716816	14.723044109859252	51.37396940148766	11.836639507936276
12	19.49549864692193	13.253363261379702	53.305202913126074	13.945935178572297
13	15.807692868282587	14.613486082972592	57.54541435283029	12.03340669591453
14	15.203787646907355	14.483765492379522	55.773295048754534	14.539151811958586
15	14.274366336777975	12.836265407356665	60.07763801639241	12.811730239472954
16	16.011990652343957	13.576935970499498	54.27081966894528	16.140253708211265
17	15.962677394122249	13.492884801313727	57.62946552201606	12.914972282547964
18	16.027051844510197	14.33218188089998	55.802445743269836	13.838320531319992
19	14.3603608855981	20.072682398324808	51.554946629936794	14.012010086140304
20	15.424361235406433	19.652426552395944	54.43649278277388	10.48671942942374
21	17.22174447472878	20.421276120236897	51.08173368897181	11.275245716062518
22	15.066536460231164	15.580317451063271	53.67808888046758	15.675057208237986
23	13.768601786937573	18.540327556637372	55.23254966549578	12.458520990929275
24	14.665957333100128	14.994388491305804	55.58721644876522	14.752437726828841
25	14.32100744800245	16.74270139486073	54.53220422976578	14.404086927371045
26	12.64484251337288	14.04820553183013	57.346217940309096	15.960734014487896
27	13.153279210210517	14.871955574341559	53.5483682898745	18.426396925573417
28	11.492661312655773	16.65962191549214	56.51153638735443	15.336180384497661
29	15.23293834142265	13.532481161363668	54.781928512780155	16.452651984433526
30	14.836003051106026	15.759351299878052	51.285059783216	18.11958586579992
31	14.587736302817413	15.986969639551663	49.83991410262016	19.58537995501076
32	16.06300436774573	17.72945240420353	50.95298478819591	15.25455843985483
33	14.248859479077089	16.411355167203524	48.39112458520991	20.948660768509477
34	15.66995583669781	17.327172819892432	49.69537524231515	17.307496101094607
35	17.537786587765453	19.796722490246662	45.21418472795114	17.45130619403674
36	15.050260655793457	20.59496567505721	43.892929499045316	20.46184417010402
37	16.49151957712059	21.03975668886978	44.84372798515258	17.62499574885705
38	16.85298818911027	19.196218183231547	42.22332347068169	21.727470156976487
39	16.78764204890515	18.962526782200587	42.499526301214125	21.75030486768014
40	16.416213616289408	19.54068222342064	44.75481836688092	19.288285793409027
41	15.235610488419887	19.866441234629082	46.4431294242252	18.454818852725833
42	16.52115611654448	19.409018253193217	47.13715887614356	16.93266675411875
43	18.29619049007176	17.671636860081524	44.38509039144524	19.647082258401475
44	18.778148639391333	18.695555004931325	44.113260165090104	18.41303619058724
45	16.797358947076916	18.607859998931144	44.940168199507355	19.65461285448459
46	20.088229435399633	20.58622046670262	41.41171955088497	17.913830547012783
47	19.406589028650274	18.77013219839963	43.620856350235876	18.20242242271422
48	20.413016756790896	19.061882066006888	39.11513066798816	21.409970509214048
49	17.509364660613038	18.474252649069363	41.62913514747822	22.387247542839376
50	18.419352174398888	17.262069602141604	42.9608360419187	21.35774218154081
51	18.969085688466528	16.952343472916574	42.028985507246375	22.04958533137052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94.0
1	418.0
2	742.0
3	22372.0
4	44002.0
5	30081.0
6	16160.0
7	15647.5
8	15135.0
9	14685.0
10	14235.0
11	13280.0
12	12325.0
13	11565.0
14	10805.0
15	10047.0
16	9289.0
17	8427.5
18	7566.0
19	6778.5
20	5991.0
21	5394.5
22	4798.0
23	4463.5
24	4129.0
25	3858.0
26	3624.5
27	3662.0
28	3683.0
29	3704.0
30	4110.5
31	4517.0
32	4729.0
33	4941.0
34	5458.0
35	5975.0
36	6601.0
37	7227.0
38	8273.5
39	9320.0
40	10989.0
41	12658.0
42	14206.0
43	15754.0
44	17126.5
45	18499.0
46	27563.0
47	36627.0
48	31195.5
49	25764.0
50	23943.5
51	22123.0
52	19567.0
53	17011.0
54	15730.0
55	14449.0
56	13577.5
57	12706.0
58	12052.5
59	11399.0
60	10604.0
61	9809.0
62	8798.0
63	7787.0
64	6811.0
65	5835.0
66	5028.0
67	4221.0
68	3621.5
69	3022.0
70	2535.0
71	2048.0
72	1732.0
73	1416.0
74	1201.5
75	827.5
76	668.0
77	526.0
78	384.0
79	260.0
80	136.0
81	106.5
82	77.0
83	60.0
84	43.0
85	27.5
86	12.0
87	11.0
88	10.0
89	7.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411654.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.251524882449324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.88389221119411	47.45229217283427
2	9.618179670190582	11.013109054235686
3	3.5724620344200417	6.135866971656136
4	1.4996941339126846	3.4343910409506138
5	0.7212011966231433	2.0644934126861054
6	0.3964729805217385	1.361920962573549
7	0.23512766221304854	0.9422992042619741
8	0.15612401235649156	0.7150670222540402
9	0.11484607926521095	0.5917601848233576
>10	0.6810594281450237	7.496973695327114
>50	0.07990717630085682	3.1865390228435473
>100	0.03628217734729676	3.7755764240768377
>500	0.002591584096235483	1.0398414241055214
>1k	0.0012957920481177416	1.8996579833480656
>5k	4.319306827059139E-4	1.5433175571563755
>10k+	4.319306827059139E-4	7.346893866866836
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29710	7.217226117078907	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	6241	1.5160790372497293	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	3926	0.9537135555587946	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	2397	0.5822851229430541	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	1359	0.33013161538573654	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	890	0.21620098432178478	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTCCTTT	822	0.19968225742978324	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC	695	0.16883110573442744	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTT	655	0.15911420756266184	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	591	0.14356717048783688	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTGCTCCTTTCGTATGCCG	552	0.1340931947703654	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	456	0.11077263915812793	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05077079294747531	0.0
2	0.0	0.0	0.0	0.35855354253815097	0.0
3	0.0	0.0	0.0	0.4778284675965738	0.0
4	0.0	0.0	0.0	1.0474816229163326	0.0
5	0.0	0.0	0.0	2.454245555733699	0.0
6	0.0	0.0	0.0	3.1419590238404096	0.0
7	0.0	0.0	0.0	3.7096687995258155	0.0
8	0.0	0.0	0.0	4.407342088258586	0.0
9	0.0	0.0	0.0	4.911892025827515	0.0
10	0.0	0.0	0.0	7.655458224625535	0.0
11	0.0	0.0	0.0	8.395885865314074	0.0
12	0.0	0.0	0.0	9.47761955428588	0.0
13	0.0	0.0	0.0	9.752364850092553	0.0
14	0.0	0.0	0.0	9.903462616663509	0.0
15	0.0	0.0	0.0	10.127680041977	0.0
16	0.0	0.0	0.0	10.292867310897016	0.0
17	0.0	0.0	0.0	10.437649093656322	0.0
18	0.0	0.0	0.0	10.609152346387987	0.0
19	0.0	0.0	0.0	10.972321415557726	0.0
20	0.0	0.0	0.0	11.104471230693738	0.0
21	0.0	0.0	0.0	11.25071054817881	0.0
22	0.0	0.0	0.0	11.4584092466003	0.0
23	0.0	0.0	0.0	11.642787389409552	0.0
24	0.0	0.0	0.0	11.794856845797684	0.0
25	0.0	0.0	0.0	11.910245011587401	0.0
26	0.0	0.0	0.0	12.037293455183237	0.0
27	0.0	0.0	0.0	12.157540070058836	0.0
28	0.0	0.0	0.0	12.269770243942729	0.0
29	0.0	0.0	0.0	12.388073479183975	0.0
30	0.0	0.0	0.0	12.52629635567734	0.0
31	0.0	0.0	0.0	12.648729272641587	0.0
32	2.4292245429414022E-4	0.0	0.0	12.799584116758249	0.0
33	2.4292245429414022E-4	0.0	0.0	12.936592380980143	0.0
34	2.4292245429414022E-4	0.0	0.0	13.089876449639746	0.0
35	2.4292245429414022E-4	0.0	0.0	13.24850481229382	0.0
36	2.4292245429414022E-4	0.0	0.0	13.391829060327362	0.0
37	2.4292245429414022E-4	0.0	0.0	13.538797145175318	0.0
38	2.4292245429414022E-4	0.0	0.0	13.729977116704806	0.0
39	2.4292245429414022E-4	0.0	0.0	14.003264877785714	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACTC	20	7.028004E-4	45.000004	9
GTATGGC	40	6.7975634E-9	45.000004	9
CGAGAGT	20	7.028004E-4	45.000004	9
GGCGCGA	20	7.028004E-4	45.000004	8
GATACGC	25	3.886428E-5	45.0	5
CCCCGTC	25	3.886428E-5	45.0	17
CGTACGG	25	3.886428E-5	45.0	2
ATACGCT	25	3.886428E-5	45.0	6
GGTATAT	30	2.1620945E-6	44.999996	8
CGATGAA	60	0.0	44.999996	19
CGTTTTT	14770	0.0	44.664864	1
GACGTGT	90	0.0	42.5	7
TGACGTG	90	0.0	42.5	6
AATGATA	1755	0.0	42.179485	2
CACCGGT	70	0.0	41.785713	16
GAATGAT	1830	0.0	41.68033	1
TACGGCG	605	0.0	41.652893	7
ATGATAC	1800	0.0	41.625	3
TACGGCT	320	0.0	41.48438	7
ATACGGC	940	0.0	41.409576	6
>>END_MODULE