FastQCFastQC Report
Sat 14 Jan 2017
SRR2933290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317306
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG122943.8744933912374804No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC116423.669013507465979No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC109833.461327551322698No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67892.1395750474305557No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGATCGTT25770.81214978601098No Hit
GAACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCT23390.7371433253704626No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTC22170.6986946354622982No Hit
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC20650.650791349675077No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTA19640.6189608768822524No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCT18700.5893364764612078No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGT8840.27859542523620734No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTT6200.19539498150050738No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATG5710.17995247489804794No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTAT5440.1714433386068968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG302.1605174E-645.0000041
GTTCTCG253.8844028E-545.01
CGACGAA207.025559E-445.019
ATTAACG207.025559E-445.044
GCAATCC207.025559E-445.035
GATTCAG207.025559E-445.09
CGTTTGG850.045.02
CCGCAAC253.8844028E-545.012
CCCGATC207.025559E-445.041
TCCGGGT253.8844028E-545.04
CGTTTTT58550.044.116141
CGATGAA4750.042.63157719
TGATACC13900.042.2482034
CCGATGA4900.042.244918
GATACCT13900.042.086335
ACGGCTG14150.041.6607788
CGGCTGT14300.041.538469
TACGGCT14200.041.5140847
ACCGGTC556.002665E-1140.90909217
CGGGCGA1100.040.9090926
Produced by FastQC (version 0.11.5)