##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933290.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	317306
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.178244344575898	31.0	28.0	31.0	25.0	34.0
2	28.09644948409422	31.0	25.0	33.0	16.0	34.0
3	28.13311125538124	31.0	25.0	33.0	16.0	34.0
4	32.847261003573834	35.0	32.0	37.0	28.0	37.0
5	34.07786490012795	35.0	33.0	37.0	30.0	37.0
6	33.23731665962825	35.0	32.0	37.0	28.0	37.0
7	33.339152111841564	35.0	32.0	35.0	28.0	37.0
8	33.094274927042036	35.0	32.0	37.0	27.0	37.0
9	34.4462789862152	37.0	34.0	39.0	28.0	39.0
10	34.13680800236995	35.0	32.0	39.0	27.0	39.0
11	34.506038335234756	37.0	33.0	39.0	27.0	39.0
12	34.609837191858965	37.0	33.0	39.0	27.0	39.0
13	34.7128828323448	37.0	33.0	39.0	27.0	39.0
14	35.56122481138081	38.0	34.0	40.0	27.0	41.0
15	35.69208587294284	38.0	33.0	40.0	27.0	41.0
16	35.73927060944325	37.0	34.0	40.0	29.0	41.0
17	35.015360566771506	37.0	33.0	40.0	27.0	41.0
18	34.90217959950332	37.0	33.0	39.0	27.0	40.0
19	34.36931227269576	37.0	32.0	38.0	27.0	40.0
20	33.61074483306335	35.0	32.0	38.0	25.0	40.0
21	34.36763880922516	35.0	33.0	38.0	27.0	40.0
22	35.020330532671935	36.0	33.0	39.0	29.0	40.0
23	35.2803004040264	36.0	34.0	39.0	30.0	40.0
24	34.93060326624772	36.0	33.0	39.0	29.0	40.0
25	33.863273937461	35.0	33.0	39.0	24.0	40.0
26	34.00080048911776	35.0	33.0	39.0	25.0	40.0
27	34.58089352234121	35.0	33.0	39.0	27.0	40.0
28	34.361446048924385	35.0	33.0	39.0	25.0	40.0
29	34.36723856466629	35.0	33.0	39.0	25.0	40.0
30	33.816149080067824	35.0	33.0	39.0	23.0	40.0
31	33.93252885227509	35.0	33.0	39.0	24.0	40.0
32	33.50300340995758	35.0	33.0	39.0	21.0	40.0
33	33.121412768746886	36.0	32.0	39.0	18.0	40.0
34	32.8524547282434	36.0	32.0	40.0	15.0	40.0
35	32.63479102191575	37.0	32.0	40.0	12.0	41.0
36	31.931712605497534	36.0	30.0	40.0	10.0	41.0
37	31.944406976231146	36.0	31.0	40.0	10.0	41.0
38	31.704729819165095	36.0	30.0	39.0	10.0	40.0
39	31.38802922100433	36.0	30.0	39.0	8.0	40.0
40	31.13302616401833	35.0	28.0	39.0	8.0	40.0
41	30.56766023964249	35.0	25.0	39.0	8.0	40.0
42	30.87300271662055	35.0	27.0	39.0	8.0	40.0
43	30.601488783697757	35.0	26.0	39.0	8.0	40.0
44	30.902683844616867	35.0	28.0	39.0	7.0	40.0
45	31.035111217562857	36.0	28.0	39.0	7.0	40.0
46	30.74056904061064	35.0	27.0	39.0	7.0	40.0
47	30.56444567704361	35.0	26.0	39.0	7.0	40.0
48	30.338783382602283	35.0	26.0	39.0	7.0	40.0
49	30.634630293785808	35.0	27.0	39.0	7.0	40.0
50	30.517513063099972	35.0	26.0	39.0	7.0	40.0
51	29.20510169993634	34.0	24.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	21.0
10	19.0
11	25.0
12	23.0
13	27.0
14	42.0
15	74.0
16	118.0
17	252.0
18	638.0
19	1072.0
20	1720.0
21	2400.0
22	3425.0
23	4910.0
24	6685.0
25	9581.0
26	12615.0
27	13908.0
28	13316.0
29	13141.0
30	14119.0
31	16602.0
32	20167.0
33	23867.0
34	27785.0
35	31826.0
36	34935.0
37	34622.0
38	25786.0
39	3580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.53480236743081	2.6567414420149635	33.15569198187239	29.65276420868184
2	30.407871266222507	23.051880519120342	33.37472345307054	13.165524761586608
3	12.65308566494173	23.16848720162871	50.82885290539731	13.349574228032246
4	11.092762191701386	2.8316514657775143	69.97031256893976	16.105273773581338
5	26.693160545341087	3.6545164604514255	51.320176737912305	18.332146256295186
6	13.787637170428546	19.94636092604615	55.40771368962453	10.858288213900776
7	54.683491645288775	1.1663819782796416	41.25512911826439	2.894997258167195
8	52.446219107107964	15.714168657384356	28.02657371748407	3.8130385180236113
9	49.63946474381197	2.8036028313363124	26.119896881874276	21.43703554297744
10	35.02486558716192	16.27986864414792	33.51433631888461	15.18092944980555
11	27.668874840059754	16.71761643334825	39.87160658796241	15.741902138629587
12	20.20068955519278	14.379494872457501	43.71269374042722	21.707121831922496
13	19.016658997938897	15.845272386907276	52.13736897505877	13.000699640095052
14	16.41790574398215	24.540033910483885	40.13854134494778	18.903519000586186
15	12.091167516529785	16.31264457652865	54.539466634731134	17.05672127221042
16	13.441598961255066	18.52155332707229	40.99103074004273	27.04581697162991
17	14.560707960139425	19.231908630785423	47.597587187131666	18.609796221943487
18	14.145020894656893	18.30661884742173	45.003876384310416	22.54448387361096
19	15.15949903247969	21.1527673602138	41.36921457520501	22.318519032101506
20	18.3460129969178	19.191569021701447	48.633180589084354	13.829237392296395
21	17.765185656747747	26.495874644664774	41.339275021587994	14.39966467699949
22	14.84623675568694	15.696204925214147	45.74669246720831	23.710865851890603
23	16.190365136492847	25.84823482694938	42.77952512716431	15.181874909393455
24	18.435201351376904	18.476801573244757	40.33393632644829	22.754060748930055
25	13.920631819127276	30.12297277706693	38.42852010362237	17.527875300183418
26	14.809994138150556	17.99997478774432	44.78831159826792	22.401719475837204
27	19.754747782897265	21.11589443628548	38.85240115220008	20.27695662861717
28	13.728073216390488	21.193422122493743	46.403471727606785	18.675032933508977
29	19.483400881168336	21.272210421485884	39.72537550503299	19.519013192312784
30	19.325509129988085	23.69857487724783	41.92199328093386	15.05392271183022
31	23.183614555035202	18.172048432743157	36.25270243865543	22.39163457356621
32	22.59963568290546	27.088992959477604	36.036822499416964	14.27454885819997
33	18.276048987412782	17.500772125330123	38.733273244123964	25.489905643133127
34	20.941614718914863	24.889538804813018	34.100836416582105	20.068010059690014
35	25.084303479921587	20.603770492836567	34.574826823318816	19.737099203923027
36	21.847995310520442	24.31123269021071	36.20511430606418	17.635657693204667
37	21.44554467926859	25.43916597858219	35.972531247439385	17.14275809470984
38	23.170062967608555	21.250149697768084	35.848045735031796	19.73174159959156
39	19.647280543071986	26.54125670488424	32.4358820822802	21.375580669763572
40	22.94787996445072	20.64001311037295	38.67717597524156	17.734930949934764
41	17.855004317598784	23.12657182656489	35.84836088822777	23.170062967608555
42	21.639363894789255	21.933086673431955	38.58326032284293	17.844289108935854
43	20.350702476473813	22.158106055353507	35.752239163457354	21.738952304715323
44	22.4899623707084	21.254876995707615	34.074363548120736	22.180797085463244
45	18.959931422664557	21.10297315525077	33.4843967652676	26.45269865681708
46	24.80161106313779	26.711754583903236	30.247458289474515	18.23917606348446
47	16.164837727619396	21.381568580486977	43.207503167289616	19.24609052460401
48	21.039627362861086	24.303669013507466	29.65276420868184	25.003939414949606
49	18.805506356639963	20.49409718063951	38.5173933048855	22.183003157835024
50	19.97062772213573	20.264665653974397	36.01381631611126	23.75089030777861
51	19.60725608718398	21.151506747429927	31.645793019987018	27.595444145399078
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	25.0
1	358.5
2	692.0
3	7403.5
4	14115.0
5	11045.0
6	7975.0
7	7901.5
8	7828.0
9	7407.0
10	6986.0
11	6507.5
12	6029.0
13	5550.0
14	5071.0
15	4638.0
16	4205.0
17	3887.5
18	3570.0
19	3350.0
20	3130.0
21	2955.5
22	2781.0
23	2789.0
24	2797.0
25	2744.0
26	2838.0
27	2985.0
28	3365.0
29	3745.0
30	4059.0
31	4373.0
32	4852.5
33	5332.0
34	5677.5
35	6023.0
36	6541.5
37	7060.0
38	7399.5
39	7739.0
40	8672.5
41	9606.0
42	10361.5
43	11117.0
44	12555.0
45	13993.0
46	37912.0
47	61831.0
48	42624.0
49	23417.0
50	21160.0
51	18903.0
52	16480.5
53	14058.0
54	13484.0
55	12910.0
56	12439.5
57	11969.0
58	10708.5
59	9448.0
60	8369.0
61	7290.0
62	6435.0
63	5580.0
64	4936.5
65	4293.0
66	3521.0
67	2749.0
68	2329.0
69	1909.0
70	1495.5
71	1082.0
72	942.5
73	803.0
74	682.0
75	406.0
76	251.0
77	206.0
78	161.0
79	134.5
80	108.0
81	89.0
82	70.0
83	49.5
84	29.0
85	20.0
86	11.0
87	7.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	317306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.44051408602937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.02924393243477	34.40774552734432
2	5.398773449831128	4.474558943900165
3	2.263811063100047	2.814404827455199
4	1.4797455830275772	2.4528567070877623
5	1.1660312688953944	2.4160467611705148
6	0.9862460473531932	2.4522325930578472
7	0.8175214030901573	2.3714955054271707
8	0.7324882088748922	2.4283750350184317
9	0.6268472939376195	2.3379186702790182
>10	3.4013238784007327	21.98403578464917
>50	0.05893379743120484	1.6661198401546444
>100	0.02831883772668285	2.0013737276557793
>500	0.0030614959704521995	0.8306811081981752
>1k	0.004592243955678299	4.13342352120604
>5k	7.653739926130499E-4	2.153300513003975
>10k+	0.0022961219778391494	11.075430934391783
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG	12294	3.8744933912374804	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC	11642	3.669013507465979	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC	10983	3.461327551322698	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6789	2.1395750474305557	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGATCGTT	2577	0.81214978601098	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCT	2339	0.7371433253704626	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTC	2217	0.6986946354622982	No Hit
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	2065	0.650791349675077	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTA	1964	0.6189608768822524	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCT	1870	0.5893364764612078	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGT	884	0.27859542523620734	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTT	620	0.19539498150050738	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATG	571	0.17995247489804794	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTAT	544	0.1714433386068968	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10778239302124763	0.0
2	0.0	0.0	0.0	0.8931441573748999	0.0
3	0.0	0.0	0.0	1.0043932355518017	0.0
4	0.0	0.0	0.0	1.9010040780823558	0.0
5	0.0	0.0	0.0	6.042432226305207	0.0
6	0.0	0.0	0.0	6.364518792585075	0.0
7	0.0	0.0	0.0	7.130656211984646	0.0
8	0.0	0.0	0.0	8.01403062028452	0.0
9	0.0	0.0	0.0	8.160892009605869	0.0
10	0.0	0.0	0.0	13.146615569828494	0.0
11	0.0	0.0	0.0	13.437186816511506	0.0
12	0.0	0.0	0.0	17.892507547919042	0.0
13	0.0	0.0	0.0	18.230666927193308	0.0
14	0.0	0.0	0.0	18.48278948396816	0.0
15	0.0	0.0	0.0	19.29683018915495	0.0
16	0.0	0.0	0.0	19.71472332700926	0.0
17	0.0	0.0	0.0	19.9157910660372	0.0
18	0.0	0.0	0.0	20.098264766502997	0.0
19	0.0	0.0	0.0	21.24731331900437	0.0
20	0.0	0.0	0.0	21.48746005433241	0.0
21	0.0	0.0	0.0	21.645666958708627	0.0
22	0.0	0.0	0.0	21.968698984576402	0.0
23	0.0	0.0	0.0	22.13068772730424	0.0
24	0.0	0.0	0.0	22.28479764013287	0.0
25	0.0	0.0	0.0	22.403610395013015	0.0
26	0.0	0.0	0.0	22.582932563519126	0.0
27	0.0	0.0	0.0	22.835370273489943	0.0
28	0.0	0.0	0.0	22.95166180280234	0.0
29	0.0	0.0	0.0	23.07709277479783	0.0
30	0.0	0.0	0.0	23.21922686617965	0.0
31	0.0	0.0	0.0	23.367033715088905	0.0
32	0.0	0.0	0.0	23.526186079053026	0.0
33	0.0	0.0	0.0	23.664538332083225	0.0
34	0.0	0.0	0.0	23.865921224307137	0.0
35	0.0	0.0	0.0	24.034528184150314	0.0
36	0.0	0.0	0.0	24.209123054716898	0.0
37	0.0	0.0	0.0	24.384348231675418	0.0
38	0.0	0.0	0.0	24.558312795850064	0.0
39	0.0	0.0	0.0	24.7814412585958	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGCG	30	2.1605174E-6	45.000004	1
GTTCTCG	25	3.8844028E-5	45.0	1
CGACGAA	20	7.025559E-4	45.0	19
ATTAACG	20	7.025559E-4	45.0	44
GCAATCC	20	7.025559E-4	45.0	35
GATTCAG	20	7.025559E-4	45.0	9
CGTTTGG	85	0.0	45.0	2
CCGCAAC	25	3.8844028E-5	45.0	12
CCCGATC	20	7.025559E-4	45.0	41
TCCGGGT	25	3.8844028E-5	45.0	4
CGTTTTT	5855	0.0	44.11614	1
CGATGAA	475	0.0	42.631577	19
TGATACC	1390	0.0	42.248203	4
CCGATGA	490	0.0	42.2449	18
GATACCT	1390	0.0	42.08633	5
ACGGCTG	1415	0.0	41.660778	8
CGGCTGT	1430	0.0	41.53846	9
TACGGCT	1420	0.0	41.514084	7
ACCGGTC	55	6.002665E-11	40.909092	17
CGGGCGA	110	0.0	40.909092	6
>>END_MODULE