FastQCFastQC Report
Sat 14 Jan 2017
SRR2933289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214854
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113135.26543606355944No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG56922.649240879853296No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC48092.2382641235443606No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC31181.4512180364340437No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGATCGTT12870.5990114217096261No Hit
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC12170.5664311579025757No Hit
GAACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCT9070.4221471324713526No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTA7370.3430236346542303No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTC6540.3043927504258706No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCT4400.2047902296443166No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGGATCGTTCGTATGCCG4330.20153220326361154No Hit
CTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGCT3500.16290131903525185Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGT3440.16010872499464754No Hit
GAATCTTTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC3220.14986921351243168No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2880.13404451394900724No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCAGGATCGTTCGTATGC2660.12380500246679141No Hit
CCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC2390.11123832928407197No Hit
GAATGACTCTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCT2310.10751487056326622No Hit
GAATCTGTCTCTTATCCACATCTGACGCAGGATCGTTCGTATGCCGTCTTC2180.10146425014195687No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGG207.0204714E-445.0000043
CCGTGCG253.8801874E-545.0000041
ACTTACA207.0204714E-445.00000428
AGACGAG207.0204714E-445.00000430
GCCGATG207.0204714E-445.0000049
CCCGTGT253.8801874E-545.00000418
CAAGGAT207.0204714E-445.00000435
ATTGGGC207.0204714E-445.0000044
CGTAACC253.8801874E-545.00000435
CCGGTGA253.8801874E-545.00000418
TGGGCGC207.0204714E-445.0000046
GTCGGGA253.8801874E-545.0000044
CCCGATG207.0204714E-445.00000417
CGTGCGG302.1572396E-644.9999962
CGTTTTT52800.044.659091
TGATACC7150.043.741264
TACGGCT6450.043.2558177
GGCGACT1200.043.12499610
CCGATGA1050.042.85714318
GATACCT7300.042.8424645
Produced by FastQC (version 0.11.5)