##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933289.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	214854
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.095264691371817	31.0	25.0	31.0	16.0	33.0
2	28.111773576475187	31.0	28.0	33.0	16.0	34.0
3	28.322944883502284	31.0	28.0	33.0	16.0	34.0
4	31.61026092136986	35.0	28.0	35.0	19.0	37.0
5	33.45745948411479	35.0	33.0	37.0	28.0	37.0
6	32.110819440177984	35.0	32.0	37.0	17.0	37.0
7	31.818607054092546	33.0	31.0	35.0	25.0	37.0
8	32.383707075502436	35.0	32.0	35.0	25.0	37.0
9	32.64534521116665	34.0	31.0	37.0	25.0	39.0
10	30.773450808455976	34.0	27.0	38.0	15.0	39.0
11	31.7176594338481	35.0	27.0	39.0	15.0	39.0
12	32.68849544341739	35.0	31.0	39.0	22.0	39.0
13	32.89794930510951	35.0	31.0	39.0	23.0	39.0
14	33.07676375585281	36.0	31.0	39.0	18.0	41.0
15	33.628626881510236	36.0	31.0	39.0	23.0	41.0
16	33.802698576707904	36.0	32.0	39.0	24.0	41.0
17	31.74264384186471	34.0	27.0	39.0	17.0	41.0
18	32.50373742169101	35.0	29.0	39.0	20.0	39.0
19	32.41645489495192	35.0	30.0	37.0	18.0	39.0
20	32.05100207582824	34.0	30.0	37.0	23.0	39.0
21	32.66395785044728	35.0	31.0	37.0	23.0	39.0
22	33.16230091131652	35.0	31.0	38.0	25.0	39.0
23	34.01534995857652	35.0	32.0	38.0	27.0	40.0
24	32.446791774879685	35.0	31.0	37.0	22.0	39.0
25	31.562828711590196	35.0	30.0	37.0	18.0	39.0
26	31.14066761614864	33.0	27.0	37.0	20.0	39.0
27	32.59967233563257	35.0	31.0	38.0	23.0	39.0
28	32.61081478585458	35.0	31.0	38.0	22.0	39.0
29	32.91987116832826	35.0	31.0	38.0	22.0	40.0
30	31.90624330941011	35.0	30.0	38.0	19.0	40.0
31	32.21022647937669	35.0	30.0	37.0	21.0	40.0
32	32.17356437394696	35.0	30.0	38.0	20.0	40.0
33	32.33188583875562	35.0	31.0	38.0	18.0	40.0
34	31.672382175803104	35.0	30.0	38.0	15.0	40.0
35	31.236155715043704	35.0	30.0	38.0	14.0	40.0
36	30.43244715015778	35.0	27.0	38.0	10.0	40.0
37	30.44769936794288	35.0	27.0	38.0	9.0	40.0
38	30.401542442775092	35.0	26.0	38.0	10.0	40.0
39	29.813245273534587	35.0	25.0	38.0	8.0	40.0
40	29.58388021633295	34.0	24.0	38.0	8.0	40.0
41	29.053478175877572	33.0	23.0	37.0	8.0	40.0
42	29.643651037448684	34.0	24.0	38.0	8.0	40.0
43	29.428025542926825	34.0	23.0	38.0	8.0	40.0
44	29.47165982481127	34.0	23.0	38.0	7.0	40.0
45	29.706735736825937	35.0	24.0	38.0	7.0	40.0
46	29.26357433419904	34.0	23.0	38.0	7.0	40.0
47	29.27239893136735	34.0	23.0	38.0	7.0	40.0
48	29.067664553603844	34.0	23.0	38.0	7.0	40.0
49	28.984026362087743	34.0	23.0	37.0	7.0	40.0
50	29.02620384074767	34.0	23.0	38.0	7.0	40.0
51	27.295824141044616	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	9.0
10	14.0
11	13.0
12	15.0
13	28.0
14	52.0
15	113.0
16	267.0
17	447.0
18	784.0
19	1201.0
20	1724.0
21	2439.0
22	3206.0
23	4367.0
24	5831.0
25	8014.0
26	10826.0
27	12258.0
28	12383.0
29	12742.0
30	13924.0
31	16158.0
32	18103.0
33	19868.0
34	21804.0
35	22527.0
36	16012.0
37	8139.0
38	1557.0
39	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.75652303424651	2.57430627309708	35.517607305426004	34.1515633872304
2	35.786627198004226	15.85402180085081	35.31002448174109	13.049326519403875
3	13.261563666489803	15.698567399257172	57.664274344438546	13.37559458981448
4	11.639531961238795	2.8107459018682452	69.33219767842348	16.217524458469473
5	22.110828748824783	3.6801735131763893	57.438074227149606	16.77092351084923
6	13.998343060869242	14.065830750183848	59.790834706358744	12.144991482588177
7	53.810029136064486	1.1565993651502882	42.02528228471427	3.0080892140709503
8	54.302921984231155	11.020041516564737	30.606365252683215	4.070671246520893
9	50.56782745492288	2.6031630781833246	30.275908291211707	16.55310117568209
10	32.47414523350741	16.11326761428691	36.535042400886184	14.877544751319501
11	24.77915235462221	17.04227056512795	43.637074478483065	14.541502601766782
12	20.169510458264682	14.532193954964768	46.626080966609884	18.67221462016067
13	17.80651046757333	15.978292235657701	53.497258603516805	12.717938693252162
14	15.181472069405272	20.405950087035848	44.979846779673636	19.432731063885242
15	11.677231980786953	16.14445158107366	55.52328557997524	16.65503085816415
16	12.981373397749168	15.75116125368855	45.79993856293111	25.467526785631172
17	14.103996202072105	17.07066193787409	50.36815698101967	18.457184879034134
18	13.75631824401687	15.926163813566422	48.94765747903227	21.369860463384438
19	15.071630037141501	19.55467433699163	45.0222011226228	20.351494503244062
20	17.778584527167286	17.175384214396754	51.26132164167295	13.78470961676301
21	17.894477179852363	22.812235285356568	44.303108157167195	14.990179377623875
22	14.918037364908262	15.695774805216567	47.804555651744906	21.581632178130263
23	15.61432414569894	23.397283736863173	44.79181211427295	16.19658000316494
24	16.94173717966619	16.920327292021557	44.50882925149171	21.62910627682054
25	13.663697208336826	24.330010146425014	43.20096437580869	18.805328269429474
26	14.326938292980348	18.392489783760134	47.571839481694546	19.70873244156497
27	16.796522289554765	19.567241010174353	43.92517709700541	19.711059603265475
28	13.73304662701183	19.73479665261061	49.38190585234624	17.150250868031314
29	18.35572062889218	19.11996053133756	44.2435328176343	18.28078602213596
30	17.157232353132827	21.970268182114367	45.6025952507284	15.269904214024407
31	20.21838085397526	18.133709402664135	40.96176938758413	20.68614035577648
32	20.66659219749225	23.878075344187216	40.8160890651326	14.63924339318793
33	16.891935919275415	17.5942733204874	42.32176268535843	23.192028074878753
34	18.81603321325179	23.877144479507013	38.72769415510067	18.579128152140523
35	21.62910627682054	20.20674504547274	38.9422584638871	19.221890213819616
36	21.54812104964301	22.176454708778984	38.97111526897335	17.30430897260465
37	20.23234382417828	24.427750937846167	38.864996695430385	16.47490854254517
38	21.081757844862093	20.809014493563073	40.41349009094548	17.69573757062936
39	17.50444487884796	24.26159159243021	37.58924665121432	20.644716877507516
40	21.942342241708324	19.613318811844323	40.923138503355766	17.52120044309159
41	17.363418879797443	20.528824224822436	40.44048516667132	21.667271728708798
42	19.924693047371704	21.052435607435747	41.144684297243714	17.878187047948842
43	19.057592597764064	20.707550243421114	39.57664274344438	20.658214415370438
44	21.687285319333128	20.805756467182366	37.35932307520456	20.14763513827995
45	18.540031835572062	21.138540590354378	36.645815297830154	23.675612276243402
46	21.441537043759947	26.314613644614482	33.647965595241416	18.595883716384147
47	16.15701825425638	21.394062945069674	43.00129390190548	19.447624898768463
48	20.431083433401287	23.075669989853576	32.74781944948663	23.745427127258512
49	18.262168728531933	20.40129576363484	38.5512952982025	22.785240209630725
50	18.877004849804983	19.881407839742337	37.481266348310946	23.760320962141734
51	18.825807292393904	20.33380807432024	34.98422184367059	25.856162789615272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30.0
1	203.0
2	376.0
3	8263.5
4	16151.0
5	11341.5
6	6532.0
7	6577.5
8	6623.0
9	6409.5
10	6196.0
11	5516.0
12	4836.0
13	4318.0
14	3800.0
15	3314.0
16	2828.0
17	2554.0
18	2280.0
19	2090.0
20	1900.0
21	1757.0
22	1614.0
23	1635.5
24	1657.0
25	1615.5
26	1621.5
27	1669.0
28	1911.0
29	2153.0
30	2379.5
31	2606.0
32	2903.0
33	3200.0
34	3445.5
35	3691.0
36	3985.5
37	4280.0
38	4484.5
39	4689.0
40	5504.5
41	6320.0
42	6914.0
43	7508.0
44	8530.0
45	9552.0
46	18824.5
47	28097.0
48	23086.0
49	18075.0
50	16606.5
51	15138.0
52	12776.0
53	10414.0
54	9586.5
55	8759.0
56	8369.0
57	7979.0
58	7203.5
59	6428.0
60	5713.5
61	4999.0
62	4494.5
63	3990.0
64	3488.5
65	2987.0
66	2470.0
67	1953.0
68	1693.0
69	1433.0
70	1160.5
71	888.0
72	779.0
73	670.0
74	564.0
75	322.0
76	186.0
77	170.5
78	155.0
79	123.5
80	92.0
81	71.5
82	51.0
83	32.5
84	14.0
85	15.0
86	16.0
87	10.0
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	214854.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.889804239157755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.47699339335517	35.32119485790351
2	6.815554777887359	5.982667299654649
3	3.0265432295146293	3.985031695942361
4	2.0148676019894167	3.5372857847654684
5	1.4602487831260142	3.2045016615934543
6	1.1230235739509433	2.957357088999972
7	0.8663930688554491	2.6618075530360152
8	0.7455010127360842	2.6175914807264467
9	0.5864325178421829	2.3164567566812813
>10	2.766731354521257	18.316624312323718
>50	0.06999013775331658	2.003220791793497
>100	0.03817643877453632	3.2570955160248354
>500	0.003181369897878026	1.0695635175514535
>1k	0.004241826530504035	4.854924739590605
>5k	0.0010604566326260089	2.649240879853296
>10k+	0.0010604566326260089	5.26543606355944
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11313	5.26543606355944	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG	5692	2.649240879853296	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC	4809	2.2382641235443606	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC	3118	1.4512180364340437	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGATCGTT	1287	0.5990114217096261	No Hit
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	1217	0.5664311579025757	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCT	907	0.4221471324713526	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTA	737	0.3430236346542303	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTC	654	0.3043927504258706	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCT	440	0.2047902296443166	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGGATCGTTCGTATGCCG	433	0.20153220326361154	No Hit
CTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGCT	350	0.16290131903525185	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGT	344	0.16010872499464754	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC	322	0.14986921351243168	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	288	0.13404451394900724	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCAGGATCGTTCGTATGC	266	0.12380500246679141	No Hit
CCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC	239	0.11123832928407197	No Hit
GAATGACTCTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCT	231	0.10751487056326622	No Hit
GAATCTGTCTCTTATCCACATCTGACGCAGGATCGTTCGTATGCCGTCTTC	218	0.10146425014195687	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1950161505022015	0.0
2	0.0	0.0	0.0	1.1123832928407198	0.0
3	0.0	0.0	0.0	1.3390488424697702	0.0
4	4.6543234010071956E-4	0.0	0.0	2.134007279361799	0.0
5	4.6543234010071956E-4	0.0	0.0	4.132108315414189	0.0
6	4.6543234010071956E-4	0.0	0.0	4.460703547525296	0.0
7	4.6543234010071956E-4	0.0	0.0	4.8954173531793685	0.0
8	4.6543234010071956E-4	0.0	0.0	5.493497910208793	0.0
9	4.6543234010071956E-4	0.0	0.0	5.676412819868376	0.0
10	4.6543234010071956E-4	0.0	0.0	9.847152019510924	0.0
11	4.6543234010071956E-4	0.0	0.0	10.592309196012176	0.0
12	4.6543234010071956E-4	0.0	0.0	13.716756495108307	0.0
13	4.6543234010071956E-4	0.0	0.0	14.017425786813371	0.0
14	4.6543234010071956E-4	0.0	0.0	14.197082670092248	0.0
15	4.6543234010071956E-4	0.0	0.0	14.789578039040464	0.0
16	4.6543234010071956E-4	0.0	0.0	15.157269587720034	0.0
17	4.6543234010071956E-4	0.0	0.0	15.50448211343517	0.0
18	4.6543234010071956E-4	0.0	0.0	15.843316857028494	0.0
19	4.6543234010071956E-4	0.0	0.0	16.89612481033632	0.0
20	4.6543234010071956E-4	0.0	0.0	17.232166959889042	0.0
21	4.6543234010071956E-4	0.0	0.0	17.60032394090871	0.0
22	4.6543234010071956E-4	0.0	0.0	18.152792128608265	0.0
23	4.6543234010071956E-4	0.0	0.0	18.508382436445213	0.0
24	4.6543234010071956E-4	0.0	0.0	18.766231952861013	0.0
25	4.6543234010071956E-4	0.0	0.0	18.989639476109357	0.0
26	4.6543234010071956E-4	0.0	0.0	19.216305025738407	0.0
27	9.308646802014391E-4	0.0	0.0	19.46391503067199	0.0
28	9.308646802014391E-4	0.0	0.0	19.66823982797621	0.0
29	9.308646802014391E-4	0.0	0.0	19.86930659889972	0.0
30	9.308646802014391E-4	0.0	0.0	20.098764742569372	0.0
31	9.308646802014391E-4	0.0	0.0	20.326361156878626	0.0
32	9.308646802014391E-4	0.0	0.0	20.53673657460415	0.0
33	9.308646802014391E-4	0.0	0.0	20.744319398289072	0.0
34	9.308646802014391E-4	0.0	0.0	20.9579528423953	0.0
35	9.308646802014391E-4	0.0	0.0	21.173913448202036	0.0
36	9.308646802014391E-4	0.0	0.0	21.389874054008768	0.0
37	9.308646802014391E-4	0.0	0.0	21.61095441555661	0.0
38	9.308646802014391E-4	0.0	0.0	21.834361938804957	0.0
39	9.308646802014391E-4	0.0	0.0	22.112225045845086	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGGG	20	7.0204714E-4	45.000004	3
CCGTGCG	25	3.8801874E-5	45.000004	1
ACTTACA	20	7.0204714E-4	45.000004	28
AGACGAG	20	7.0204714E-4	45.000004	30
GCCGATG	20	7.0204714E-4	45.000004	9
CCCGTGT	25	3.8801874E-5	45.000004	18
CAAGGAT	20	7.0204714E-4	45.000004	35
ATTGGGC	20	7.0204714E-4	45.000004	4
CGTAACC	25	3.8801874E-5	45.000004	35
CCGGTGA	25	3.8801874E-5	45.000004	18
TGGGCGC	20	7.0204714E-4	45.000004	6
GTCGGGA	25	3.8801874E-5	45.000004	4
CCCGATG	20	7.0204714E-4	45.000004	17
CGTGCGG	30	2.1572396E-6	44.999996	2
CGTTTTT	5280	0.0	44.65909	1
TGATACC	715	0.0	43.74126	4
TACGGCT	645	0.0	43.255817	7
GGCGACT	120	0.0	43.124996	10
CCGATGA	105	0.0	42.857143	18
GATACCT	730	0.0	42.842464	5
>>END_MODULE