##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933288.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645361
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.62962280026218	31.0	28.0	33.0	25.0	34.0
2	29.006780701033996	31.0	28.0	33.0	16.0	34.0
3	28.95013023718508	31.0	28.0	34.0	16.0	34.0
4	33.33315617150711	35.0	32.0	37.0	28.0	37.0
5	34.369154628184845	35.0	33.0	37.0	32.0	37.0
6	33.92704858211141	35.0	33.0	37.0	28.0	37.0
7	33.80499751301984	35.0	33.0	37.0	29.0	37.0
8	33.79623497546334	35.0	33.0	37.0	28.0	37.0
9	34.95364145028906	37.0	34.0	39.0	28.0	39.0
10	34.89534539583272	37.0	34.0	39.0	28.0	39.0
11	35.24143851270839	37.0	34.0	39.0	29.0	39.0
12	35.34569334062641	37.0	34.0	39.0	30.0	39.0
13	35.5528533642411	37.0	34.0	39.0	30.0	39.0
14	36.325154448440486	38.0	35.0	40.0	30.0	41.0
15	36.68443708250111	38.0	35.0	41.0	30.0	41.0
16	36.65008111739011	38.0	34.0	41.0	31.0	41.0
17	35.87683792482037	38.0	34.0	40.0	27.0	41.0
18	35.60832774214742	38.0	34.0	39.0	28.0	40.0
19	34.8609956288031	37.0	34.0	38.0	27.0	40.0
20	34.12858229734986	35.0	33.0	38.0	27.0	40.0
21	34.36307740938793	35.0	33.0	38.0	29.0	40.0
22	34.76995046183454	35.0	33.0	39.0	30.0	40.0
23	35.140671655089164	35.0	34.0	39.0	30.0	40.0
24	34.535565365741036	35.0	33.0	38.0	27.0	40.0
25	33.505050661567715	35.0	33.0	38.0	24.0	40.0
26	33.49441630343327	35.0	32.0	38.0	24.0	40.0
27	34.36962878141071	35.0	33.0	38.0	27.0	40.0
28	34.11418570381539	35.0	33.0	39.0	25.0	40.0
29	34.0032555422469	35.0	33.0	39.0	24.0	40.0
30	33.2419916914719	35.0	33.0	39.0	21.0	40.0
31	33.12365172360896	35.0	33.0	38.0	21.0	40.0
32	32.34680124767378	35.0	31.0	38.0	16.0	40.0
33	31.453164972782677	35.0	30.0	39.0	12.0	40.0
34	30.646188722281018	35.0	25.0	39.0	8.0	40.0
35	30.064272554430776	35.0	22.0	39.0	7.0	41.0
36	29.39745041922273	35.0	20.0	39.0	7.0	40.0
37	29.223194460154858	35.0	18.0	39.0	7.0	40.0
38	29.066203256781865	35.0	18.0	39.0	7.0	40.0
39	28.61227901902966	35.0	18.0	39.0	7.0	40.0
40	28.526167524842684	35.0	18.0	39.0	7.0	40.0
41	28.12445592466852	34.0	17.0	39.0	7.0	40.0
42	28.204028442995472	35.0	15.0	39.0	7.0	40.0
43	28.08431405058564	35.0	15.0	39.0	7.0	40.0
44	28.14479182968912	35.0	15.0	39.0	7.0	40.0
45	28.26322321925248	35.0	15.0	39.0	7.0	40.0
46	28.043949045572944	35.0	15.0	39.0	7.0	40.0
47	27.96195462694523	34.0	15.0	39.0	7.0	40.0
48	27.81951961770234	34.0	15.0	38.0	7.0	40.0
49	28.007721259884004	34.0	15.0	39.0	7.0	40.0
50	27.98390513216634	34.0	15.0	39.0	7.0	40.0
51	27.009049198820506	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	13.0
10	21.0
11	26.0
12	54.0
13	77.0
14	105.0
15	180.0
16	302.0
17	692.0
18	1408.0
19	2611.0
20	4136.0
21	6337.0
22	9332.0
23	13791.0
24	20192.0
25	29496.0
26	37789.0
27	38980.0
28	34393.0
29	30093.0
30	28725.0
31	29946.0
32	32968.0
33	38486.0
34	44890.0
35	53098.0
36	59557.0
37	64709.0
38	54092.0
39	8853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.967610376208043	2.2170537110237523	31.14241486547839	43.67292104728981
2	42.38139583891806	13.733243874358692	30.822129009964964	13.06323127675828
3	10.455078630409957	14.020060090399017	62.07502467611151	13.449836603079518
4	9.326717914469576	2.391839606049947	73.31834430651992	14.96309817296056
5	18.211047770162747	2.83577718517233	63.508795852243935	15.444379192420987
6	12.356649998992813	11.820515959284803	65.63551252709723	10.187321514625147
7	49.16023744849782	0.831937473754999	47.815253788189864	2.1925712895573173
8	48.91308895331451	8.43156001059872	40.103600930332014	2.5517501057547634
9	46.07328301524263	2.4920935724346527	39.20611874594219	12.228504666380521
10	27.045018214611666	18.703330384079607	44.32759339346505	9.924058007843673
11	18.826672203619367	15.015936816758371	53.75534003449233	12.402050945129934
12	15.03623553329067	14.175476981100502	54.86464165017719	15.923645835431643
13	14.518695737734385	14.88190330683137	59.6066387649703	10.992762190463942
14	13.14365138271448	19.361256722981402	52.94292651709663	14.552165377207485
15	11.075506576939107	15.330334494957084	60.14695650961245	13.447202418491354
16	12.91292780319852	17.181391500261096	52.20008646323531	17.70559423330508
17	12.962977310373574	17.092758936471213	56.27052146008204	13.673742293073179
18	13.364458032016188	15.919926986601299	55.18663197807119	15.528983003311325
19	12.64981924845164	18.15898388653792	52.62682436651735	16.56437249849309
20	15.064746707656646	17.559784368748655	56.33839045123582	11.037078472358882
21	14.679226045577593	20.85406462429555	53.17148076812822	11.295228561998634
22	13.678855710214902	14.203523299362683	55.47437790631909	16.643243084103315
23	13.374684866299638	20.056061646117445	54.49244686307354	12.076806624509382
24	14.820232397061488	16.172498802995534	53.04271562737755	15.964553172565433
25	12.132124500860758	23.332367465651007	50.92622578680769	13.60928224668054
26	12.886276053247716	16.591179200478493	54.16968177500655	16.352862971267243
27	15.014697200481592	18.152940757188613	51.1070548111832	15.725307231146598
28	11.539432968524594	18.5832425572664	54.84341322143731	15.033911252771704
29	14.709596644358738	18.77026966302581	51.617621765182584	14.902511927432863
30	13.169993228596088	22.41024790776015	52.22906249370507	12.190696369938687
31	17.68219647608083	17.029693458389954	49.22376778268287	16.064342282846344
32	16.297854998985066	21.070377664593927	49.785004051995706	12.846763284425306
33	15.01330263217021	21.607131512440326	44.57706616916733	18.80249968622213
34	16.509364526210913	23.252567167833195	43.51750415658833	16.720564149367565
35	14.355531245303016	25.17459220498295	41.34135778269837	19.12851876701567
36	20.486518398229826	25.008328671859626	38.242626994813754	16.262525935096793
37	16.10974322898347	25.34116564217547	42.519458101744604	16.029633027096462
38	19.746777385060454	26.307291577892062	37.21126625253153	16.734664784515953
39	19.21312257790601	23.134028861365962	39.231530879616216	18.421317681111812
40	18.02758456119908	25.775806099221988	38.325526333323516	17.871083006255414
41	20.091080805936524	25.51796591365143	35.98482089869081	18.40613238172124
42	17.318679002914646	23.98440562723809	39.82856106892111	18.86835430092615
43	21.123526212460934	23.706266725135233	36.2538176307524	18.91638943165143
44	19.489711959662888	24.49419782106449	36.33485754484699	19.68123267442563
45	17.983578183373336	22.911207835614487	37.02284457846074	22.082369402551443
46	21.065109295417603	24.47746300132794	36.30448694606584	18.152940757188613
47	15.932168197334514	23.481121418864788	42.82006504886412	17.76664533493657
48	18.48500296733146	24.181659567280946	36.31207959576113	21.02125786962646
49	16.886672730456286	22.217642528755224	40.69117904552646	20.204505695262032
50	18.447349622924225	22.033559511653166	38.99476417075095	20.524326694671664
51	17.58488659835348	21.863267225630306	37.44415916053186	23.10768701548436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	1035.0
2	1989.0
3	25693.5
4	49398.0
5	37127.5
6	24857.0
7	23770.5
8	22684.0
9	21982.5
10	21281.0
11	20515.5
12	19750.0
13	18687.5
14	17625.0
15	16465.5
16	15306.0
17	14361.0
18	13416.0
19	12315.0
20	11214.0
21	10346.0
22	9478.0
23	8981.0
24	8484.0
25	8286.0
26	8351.0
27	8614.0
28	9192.0
29	9770.0
30	10686.5
31	11603.0
32	12692.0
33	13781.0
34	14833.5
35	15886.0
36	17155.5
37	18425.0
38	19141.0
39	19857.0
40	20778.0
41	21699.0
42	22675.0
43	23651.0
44	25245.0
45	26839.0
46	55371.0
47	83903.0
48	59495.5
49	35088.0
50	32293.0
51	29498.0
52	26081.0
53	22664.0
54	21046.5
55	19429.0
56	18280.0
57	17131.0
58	15408.5
59	13686.0
60	12240.0
61	10794.0
62	9018.5
63	7243.0
64	6054.5
65	4866.0
66	3847.5
67	2829.0
68	2226.5
69	1624.0
70	1297.5
71	971.0
72	830.0
73	689.0
74	608.5
75	436.5
76	345.0
77	261.5
78	178.0
79	122.0
80	66.0
81	45.0
82	24.0
83	20.0
84	16.0
85	11.5
86	7.0
87	5.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	645361.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.67583405450018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.41591584129249	40.7514360651399
2	7.442084406602367	7.542676688171299
3	3.864146371340946	5.874565208291183
4	2.5846797461621205	5.23923207602157
5	1.7449551700238246	4.421352931433473
6	1.1639967629339283	3.539190407904908
7	0.7659530423897664	2.7170716488778344
8	0.5438281528926023	2.2047156184120693
9	0.36655725857069654	1.671803532612102
>10	1.0248774243715684	7.845558934956733
>50	0.03979305572417383	1.4723938420039087
>100	0.03792775294139152	3.4419921469221073
>500	0.0018652993249863444	0.5967716153671019
>1k	0.0021761825458174017	2.6996511090102056
>5k	0.0	0.0
>10k+	0.0012435328833242296	9.981588174875618
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22303	3.4558952276322863	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	14726	2.281823661485587	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	14575	2.258425904261336	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	11754	1.8213062146612515	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	3351	0.5192442679368602	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTC	2784	0.43138646431996974	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	2569	0.3980717768814664	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT	2504	0.38799989463261647	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT	2326	0.3604184324742276	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA	1995	0.30912930902239216	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT	1607	0.24900791959848828	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGT	905	0.1402315913109097	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5495203459769028E-4	0.0	0.0	0.044161329860341735	0.0
2	1.5495203459769028E-4	0.0	0.0	0.502819352269505	0.0
3	1.5495203459769028E-4	0.0	0.0	0.5847889785716831	0.0
4	1.5495203459769028E-4	0.0	0.0	1.2185428000762364	0.0
5	1.5495203459769028E-4	0.0	0.0	3.900917470996853	0.0
6	1.5495203459769028E-4	1.5495203459769028E-4	0.0	4.27140778571993	0.0
7	1.5495203459769028E-4	1.5495203459769028E-4	0.0	4.757647270287483	0.0
8	1.5495203459769028E-4	1.5495203459769028E-4	0.0	5.341661488686177	0.0
9	1.5495203459769028E-4	1.5495203459769028E-4	0.0	5.45957998701502	0.0
10	1.5495203459769028E-4	1.5495203459769028E-4	0.0	8.141954657935637	0.0
11	1.5495203459769028E-4	1.5495203459769028E-4	0.0	8.28187634517735	0.0
12	1.5495203459769028E-4	1.5495203459769028E-4	0.0	10.479096195772598	0.0
13	1.5495203459769028E-4	1.5495203459769028E-4	0.0	10.647219153311093	0.0
14	1.5495203459769028E-4	1.5495203459769028E-4	0.0	10.771025828954647	0.0
15	1.5495203459769028E-4	1.5495203459769028E-4	0.0	11.187691849987836	0.0
16	1.5495203459769028E-4	1.5495203459769028E-4	0.0	11.408653451324142	0.0
17	1.5495203459769028E-4	1.5495203459769028E-4	0.0	11.52424766913402	0.0
18	1.5495203459769028E-4	1.5495203459769028E-4	0.0	11.607147007643784	0.0
19	1.5495203459769028E-4	1.5495203459769028E-4	0.0	12.150253888908688	0.0
20	1.5495203459769028E-4	1.5495203459769028E-4	0.0	12.269257051479714	0.0
21	1.5495203459769028E-4	1.5495203459769028E-4	0.0	12.341929555706031	0.0
22	1.5495203459769028E-4	1.5495203459769028E-4	0.0	12.49626178216533	0.0
23	1.5495203459769028E-4	1.5495203459769028E-4	0.0	12.58287996950544	0.0
24	1.5495203459769028E-4	1.5495203459769028E-4	0.0	12.667483780395779	0.0
25	1.5495203459769028E-4	1.5495203459769028E-4	0.0	12.7398463805529	0.0
26	1.5495203459769028E-4	1.5495203459769028E-4	0.0	12.824140287374043	0.0
27	1.5495203459769028E-4	1.5495203459769028E-4	0.0	12.933846327869208	0.0
28	1.5495203459769028E-4	1.5495203459769028E-4	0.0	13.006363880060928	0.0
29	1.5495203459769028E-4	1.5495203459769028E-4	0.0	13.08399484939437	0.0
30	1.5495203459769028E-4	1.5495203459769028E-4	0.0	13.18796766460942	0.0
31	1.5495203459769028E-4	1.5495203459769028E-4	0.0	13.274275947880334	0.0
32	1.5495203459769028E-4	1.5495203459769028E-4	0.0	13.374994770368833	0.0
33	1.5495203459769028E-4	1.5495203459769028E-4	0.0	13.461612957708942	0.0
34	1.5495203459769028E-4	1.5495203459769028E-4	0.0	13.587898865906059	0.0
35	1.5495203459769028E-4	1.5495203459769028E-4	0.0	13.692026633155706	0.0
36	1.5495203459769028E-4	1.5495203459769028E-4	0.0	13.793210311747998	0.0
37	1.5495203459769028E-4	1.5495203459769028E-4	0.0	13.904775776658335	0.0
38	1.5495203459769028E-4	1.5495203459769028E-4	0.0	14.02920226044028	0.0
39	1.5495203459769028E-4	1.5495203459769028E-4	0.0	14.181675062484407	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGCG	35	1.2108285E-7	45.000004	1
TCGCCTG	25	3.8888975E-5	45.0	1
CTCGTAA	20	7.030984E-4	45.0	33
ATCCGGC	20	7.030984E-4	45.0	6
CGAATAG	20	7.030984E-4	45.0	26
AACGACG	20	7.030984E-4	45.0	11
TCGTAAC	20	7.030984E-4	45.0	34
CGTTTTT	20465	0.0	44.54923	1
CCGATGA	370	0.0	43.175674	18
TACGGCT	1495	0.0	43.04348	7
CGATGAA	385	0.0	42.66234	19
TGATACC	1735	0.0	42.536022	4
CGGCTGT	1520	0.0	42.48355	9
ACGGCTG	1510	0.0	42.46689	8
GATACCT	1760	0.0	42.1875	5
ATACCTG	1785	0.0	41.596638	6
GCCGATT	65	0.0	41.53846	9
TTCCGCG	60	3.6379788E-12	41.249996	1
ACGGGAT	60	3.6379788E-12	41.249996	5
TTACGGG	60	3.6379788E-12	41.249996	3
>>END_MODULE