##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933287.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431682
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.955578411886528	31.0	28.0	31.0	19.0	33.0
2	29.328063250262925	31.0	30.0	33.0	16.0	34.0
3	29.48632789877734	31.0	30.0	33.0	16.0	34.0
4	32.48765989779513	35.0	32.0	37.0	19.0	37.0
5	33.81467144796401	35.0	33.0	37.0	30.0	37.0
6	33.22825135168944	35.0	33.0	37.0	26.0	37.0
7	32.30758289666931	35.0	32.0	35.0	25.0	37.0
8	33.338531604282785	35.0	33.0	37.0	28.0	37.0
9	33.22218901876845	35.0	31.0	38.0	25.0	39.0
10	31.965513966299266	35.0	28.0	39.0	15.0	39.0
11	33.03321426420374	37.0	31.0	39.0	17.0	39.0
12	33.67267340310692	37.0	31.0	39.0	25.0	39.0
13	33.93982607567607	37.0	32.0	39.0	26.0	39.0
14	33.943787324929	37.0	32.0	40.0	21.0	41.0
15	34.68733002534273	37.0	32.0	40.0	25.0	41.0
16	34.913524307244685	37.0	32.0	40.0	26.0	41.0
17	32.946632011527	36.0	30.0	40.0	18.0	41.0
18	33.553856774199524	36.0	31.0	39.0	24.0	39.0
19	33.30895427652763	37.0	32.0	37.0	23.0	39.0
20	32.871729652846305	35.0	32.0	37.0	25.0	39.0
21	32.99696535875946	35.0	32.0	37.0	25.0	39.0
22	32.99279098966369	35.0	31.0	37.0	25.0	39.0
23	34.10674755954615	35.0	33.0	38.0	27.0	40.0
24	32.27217488799626	35.0	31.0	37.0	21.0	39.0
25	31.323495072761894	34.0	29.0	37.0	18.0	39.0
26	30.416792917008355	33.0	25.0	37.0	18.0	39.0
27	32.44753313781904	34.0	30.0	37.0	22.0	39.0
28	32.53078655121131	35.0	31.0	37.0	21.0	39.0
29	32.569972804054835	35.0	31.0	38.0	21.0	40.0
30	31.503868588451684	35.0	29.0	37.0	18.0	40.0
31	31.961819116849902	35.0	30.0	37.0	21.0	40.0
32	31.709489855958786	35.0	30.0	37.0	18.0	40.0
33	31.330261627772295	35.0	30.0	38.0	15.0	40.0
34	30.140971826483383	35.0	25.0	38.0	9.0	40.0
35	29.446252102241928	35.0	23.0	38.0	7.0	40.0
36	28.793496138361107	34.0	21.0	38.0	7.0	40.0
37	28.797098327009234	35.0	21.0	38.0	7.0	40.0
38	28.87456970640425	35.0	21.0	38.0	7.0	40.0
39	28.412947493756977	34.0	20.0	38.0	7.0	40.0
40	28.363049652290343	34.0	19.0	38.0	7.0	40.0
41	27.948547773592598	33.0	18.0	38.0	7.0	40.0
42	28.214861865910553	34.0	18.0	38.0	7.0	40.0
43	28.218774468242827	34.0	18.0	38.0	7.0	40.0
44	28.140927812602797	34.0	18.0	38.0	7.0	40.0
45	28.34116085451791	34.0	18.0	38.0	7.0	40.0
46	28.073104739136678	34.0	18.0	38.0	7.0	40.0
47	28.126662682252213	34.0	18.0	38.0	7.0	40.0
48	27.85348705760259	34.0	18.0	38.0	7.0	40.0
49	27.616268456873346	33.0	18.0	37.0	7.0	40.0
50	27.836916989821212	34.0	18.0	38.0	7.0	40.0
51	26.527212160803554	31.0	16.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	11.0
11	24.0
12	32.0
13	67.0
14	80.0
15	184.0
16	388.0
17	754.0
18	1469.0
19	2317.0
20	3806.0
21	5534.0
22	7648.0
23	10622.0
24	14849.0
25	20487.0
26	25094.0
27	26035.0
28	24163.0
29	23623.0
30	24788.0
31	27660.0
32	31635.0
33	36251.0
34	40721.0
35	45166.0
36	34489.0
37	19472.0
38	4234.0
39	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.68064918157347	2.2416964339490644	34.653518098971006	44.424136285506464
2	44.41255368535172	8.971418775858155	33.618496949143115	12.997530589647008
3	11.215200077835073	8.95219165960128	66.19479153636242	13.637816726201232
4	10.182263796035045	2.518057273641245	71.40742491000319	15.892254020320514
5	15.575354080086731	3.021668728369494	65.97194230938514	15.431034882158626
6	13.117294675247058	8.07376726386553	67.47466885346157	11.334269207425837
7	51.41191895886323	0.8791193517450345	45.37437280220162	2.3345888871901073
8	53.06753582498228	5.538567741995265	38.92935077209613	2.46454566092633
9	49.342571615216755	2.598903822721355	38.72920344142216	9.32932112063973
10	26.237600826534347	20.400665304552888	43.64254242706437	9.719191441848398
11	17.18417724158061	16.002057069787483	54.592732613358905	12.221033075273002
12	14.778934493446567	15.132898754175528	55.26915646239593	14.819010289981977
13	14.104132208431205	15.886694372246238	58.57320898253807	11.435964436784484
14	12.908344568455481	17.461927993291358	54.43937898731011	15.190348450943056
15	11.495267349576773	16.164676775960082	58.72517269656831	13.614883177894841
16	13.304701145750808	16.408374683215886	53.3693783850149	16.91754578601841
17	13.184705408147664	16.50844834855287	56.13090191390885	14.175944329390616
18	13.319526873948877	15.818588683336346	55.417413744376645	15.444470698338128
19	13.12702405937704	18.010479936620012	53.13749472991694	15.725001274086017
20	15.143786398320985	17.673889576123162	55.77971747721703	11.402606548338824
21	15.138226750246709	19.347575298483605	53.23015553115488	12.284042420114808
22	14.10088908038788	15.45304182245264	54.652730482160486	15.793338614999003
23	13.337827382193373	19.72447310751896	53.97792819714512	12.959771313142546
24	13.730245875436085	16.761643987935564	54.059238050231414	15.44887208639693
25	12.63800668084377	20.4541769172678	52.05382665943913	14.853989742449302
26	13.123317627327523	18.009553328607634	53.58643631191479	15.280692732150056
27	13.61441987388865	18.718871762084127	52.21158167354674	15.455126690480492
28	12.009998100453574	18.67578448950848	54.642769446027394	14.671447964010545
29	14.134478620836635	18.22290482345801	52.58732122256661	15.055295333138746
30	13.242618408921384	20.44884892119662	53.14490759401597	13.163625075866031
31	15.676354353436095	18.217808479389923	50.757270398117136	15.348566769056852
32	15.149114394392168	20.444215881134724	50.90900246014427	13.497667264328836
33	14.350146635717959	20.786134237702754	47.41569025347362	17.448028873105667
34	15.454431734471207	23.277551530988088	45.20526683994237	16.062749894598337
35	13.77518636403649	25.198641592653853	42.96125388596235	18.064918157347307
36	18.541194675710358	24.390407753855847	40.63546777488985	16.43292979554394
37	16.14637626771559	24.965368025537316	42.95430432586951	15.93395138087759
38	18.35842124526851	25.183120908446494	40.53539410955286	15.923063736732132
39	17.807784433911998	23.363494424136285	41.020473404033524	17.80824773791819
40	17.527485510167207	24.932010137091655	40.189074364926036	17.351429987815102
41	18.728369494211016	24.663293813501603	38.938153548213734	17.670183144073647
42	17.02734883548538	23.9363698277899	41.00124628777665	18.035035048948068
43	19.323251838158644	23.66186220412248	38.95506414443965	18.05982181327922
44	18.556252055911525	24.443919366570764	38.69839372501054	18.30143485250717
45	17.349576771790346	23.180489341691338	39.2105763038533	20.259357582665018
46	18.66049545730422	24.453417098697653	38.63121464411303	18.2548727998851
47	15.817662075323966	23.64819473593988	42.92465286947336	17.60949031926279
48	17.45428347718923	23.551364198646226	38.974522912699626	20.019829411464922
49	16.38196635486307	22.535106861069025	41.16525590596782	19.917670878100083
50	17.522157514096023	22.2471634212221	40.47724945677606	19.75342960790582
51	16.892990673690356	21.792430539146874	39.71557767060012	21.599001116562654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	385.0
2	682.0
3	22325.5
4	43969.0
5	30031.0
6	16093.0
7	15185.0
8	14277.0
9	13798.0
10	13319.0
11	12926.5
12	12534.0
13	11866.5
14	11199.0
15	10490.0
16	9781.0
17	9020.0
18	8259.0
19	7538.5
20	6818.0
21	6342.0
22	5866.0
23	5543.0
24	5220.0
25	5202.5
26	5337.5
27	5490.0
28	6007.0
29	6524.0
30	7167.5
31	7811.0
32	8840.0
33	9869.0
34	10629.0
35	11389.0
36	12451.5
37	13514.0
38	14021.5
39	14529.0
40	15161.0
41	15793.0
42	16507.5
43	17222.0
44	18166.0
45	19110.0
46	29173.0
47	39236.0
48	32700.0
49	26164.0
50	24111.0
51	22058.0
52	19432.0
53	16806.0
54	15116.5
55	13427.0
56	12296.5
57	11166.0
58	9846.0
59	8526.0
60	7616.5
61	6707.0
62	5670.5
63	4634.0
64	3946.0
65	3258.0
66	2585.5
67	1913.0
68	1513.0
69	1113.0
70	904.0
71	695.0
72	615.5
73	536.0
74	471.5
75	332.0
76	257.0
77	196.0
78	135.0
79	96.0
80	57.0
81	38.0
82	19.0
83	19.5
84	20.0
85	11.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431682.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.393798247051805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92192798700525	41.87413370466557
2	8.384007497391162	8.785399946401645
3	3.9145666356964877	6.152970436059664
4	2.4081399662206406	5.046863981633056
5	1.6210595215995909	4.246673276057565
6	1.0829131708227462	3.404276051469726
7	0.7326609711095494	2.687082377266195
8	0.5168806487342832	2.166507234207143
9	0.3693228640500485	1.7415204864355425
>10	0.9595713431102068	7.278247734987092
>50	0.04831261515655515	1.7390139863973264
>100	0.03612158142533782	3.458865935933681
>500	0.0013545593034501682	0.45444781225146036
>1k	0.0022575988390836138	2.73354735583843
>5k	4.515197678167227E-4	1.4944023060866607
>10k+	4.515197678167227E-4	6.736047374309257
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28474	6.59605913612335	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	6317	1.4633457035502986	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	4262	0.9873008371903392	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	3942	0.9131721961999805	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	1214	0.28122553175717313	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	1100	0.2548172034043578	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGTACCGT	1037	0.24022312720938097	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTC	834	0.1931977705811222	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTA	549	0.12717694969908405	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAAGTACCGTCGTATGCCG	538	0.12462877766504048	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT	474	0.10980304946696874	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCT	437	0.10123192535245852	No Hit
GAATCTTTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	433	0.10030531734007903	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06092447681395101	0.0
2	0.0	0.0	0.0	0.430177769747175	0.0
3	0.0	0.0	0.0	0.5524900273812667	0.0
4	0.0	0.0	0.0	1.0384959298743057	0.0
5	0.0	0.0	0.0	2.3151301189301385	0.0
6	0.0	0.0	0.0	2.680213675807655	0.0
7	0.0	0.0	0.0	3.0193522083385456	0.0
8	0.0	0.0	0.0	3.456016234172377	0.0
9	0.0	0.0	0.0	3.6684411210103733	0.0
10	0.0	0.0	0.0	5.549687038143819	0.0
11	0.0	0.0	0.0	5.9200985910925175	0.0
12	0.0	0.0	0.0	7.324604685856719	0.0
13	0.0	0.0	0.0	7.484444567992179	0.0
14	0.0	0.0	0.0	7.584981537335353	0.0
15	0.0	0.0	0.0	7.835397352680909	0.0
16	0.0	0.0	0.0	8.009368007005156	0.0
17	0.0	0.0	0.0	8.168744585134428	0.0
18	0.0	0.0	0.0	8.329047771276079	0.0
19	0.0	0.0	0.0	8.75575076097683	0.0
20	0.0	0.0	0.0	8.918833771155619	0.0
21	0.0	0.0	0.0	9.076588785263226	0.0
22	0.0	0.0	0.0	9.304071052302389	0.0
23	0.0	0.0	0.0	9.468543974499747	0.0
24	0.0	0.0	0.0	9.602438832288582	0.0
25	0.0	0.0	0.0	9.728225869969098	0.0
26	0.0	0.0	0.0	9.840808743473206	0.0
27	0.0	0.0	0.0	9.954549876992786	0.0
28	0.0	0.0	0.0	10.066669446490703	0.0
29	0.0	0.0	0.0	10.18921335612789	0.0
30	0.0	0.0	0.0	10.333069250049805	0.0
31	0.0	0.0	0.0	10.457698027714846	0.0
32	0.0	0.0	0.0	10.578852025333463	0.0
33	0.0	0.0	0.0	10.70139593497065	0.0
34	0.0	0.0	0.0	10.838070616796623	0.0
35	0.0	0.0	0.0	10.984243030749488	0.0
36	0.0	0.0	0.0	11.107945200402147	0.0
37	0.0	0.0	0.0	11.255739178376675	0.0
38	0.0	0.0	0.0	11.407702892406911	0.0
39	0.0	0.0	0.0	11.605302051046836	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	25	3.886744E-5	45.0	4
GCACGGG	25	3.886744E-5	45.0	3
CCGCTGA	20	7.028386E-4	45.0	18
CCCGTTG	20	7.028386E-4	45.0	1
GTTCGCG	25	3.886744E-5	45.0	1
GCGATCG	25	3.886744E-5	45.0	9
CGATCGT	20	7.028386E-4	45.0	10
GTACGGG	20	7.028386E-4	45.0	3
CGTTTTT	15165	0.0	44.569733	1
TTCTTCG	105	0.0	42.857143	1
ACGGCTG	540	0.0	41.25	8
ACACGAC	105	0.0	40.714287	26
GACACGA	105	0.0	40.714287	25
GCACGCC	50	1.0786607E-9	40.5	25
GCGTTTG	50	1.0786607E-9	40.5	1
TACGGCT	550	0.0	40.5	7
TGGGCGA	400	0.0	40.5	6
CGGTAGG	50	1.0786607E-9	40.5	31
CGCCGGT	50	1.0786607E-9	40.5	28
GTTTTTT	16835	0.0	40.442528	2
>>END_MODULE